{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T14:56:01Z","timestamp":1775055361568,"version":"3.50.1"},"reference-count":54,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,22]],"date-time":"2022-01-22T00:00:00Z","timestamp":1642809600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005363","name":"University of Buenos Aires","doi-asserted-by":"publisher","award":["20020190100013BA"],"award-info":[{"award-number":["20020190100013BA"]}],"id":[{"id":"10.13039\/501100005363","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agencia de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","award":["PICT-2017- 1136"],"award-info":[{"award-number":["PICT-2017- 1136"]}]},{"name":"Ministry of Education and Training - Vietnam International Education Development","award":["933\/Q\u0110-BGD\u0110T"],"award-info":[{"award-number":["933\/Q\u0110-BGD\u0110T"]}]},{"name":"Friedrich-Loeffler-Institut","award":["Intramural"],"award-info":[{"award-number":["Intramural"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>New World arenaviruses are rodent-transmitted viruses and include a number of pathogens that are responsible for causing severe human disease. This includes Jun\u00edn virus (JUNV), which is the causative agent of Argentine hemorrhagic fever. The wild nature and mobility of the rodent reservoir host makes it difficult to control the disease, and currently passive immunization with high-titer neutralizing antibody-containing plasma from convalescent patients is the only specific therapy. However, dwindling supplies of naturally available convalescent plasma, and challenges in developing similar resources for other closely related viruses, have made the development of alternative antibody-based therapeutic approaches of critical importance. In this study, we sought to induce a neutralizing antibody response in rabbits against the receptor-binding subunit of the viral glycoprotein, GP1, and the specific peptide sequences in GP1 involved in cellular receptor contacts. While these specific receptor-interacting peptides did not efficiently induce the production of neutralizing antibodies when delivered as a particulate antigen (as part of hepatitis B virus core-like particles), we showed that recombinant JUNV GP1 purified from transfected mammalian cells induced virus-neutralizing antibodies at high titers in rabbits. Further, neutralization was observed across a range of unrelated JUNV strains, a feature that is critical for effectiveness in the field. These results underscore the potential of GP1 alone to induce a potent neutralizing antibody response and highlight the importance of epitope presentation. In addition, effective virus neutralization by rabbit antibodies supports the potential applicability of this species for the future development of immunotherapeutics (e.g., based on humanized monoclonal antibodies). Such information can be applied in the design of vaccines and immunogens for both prevention and specific therapies against this and likely also other closely related pathogenic New World arenaviruses.<\/jats:p>","DOI":"10.3390\/vaccines10020173","type":"journal-article","created":{"date-parts":[[2022,1,23]],"date-time":"2022-01-23T20:36:27Z","timestamp":1642970187000},"page":"173","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Immunization with GP1 but Not Core-like Particles Displaying Isolated Receptor-Binding Epitopes Elicits Virus-Neutralizing Antibodies against Jun\u00edn Virus"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1810-1347","authenticated-orcid":false,"given":"Gleyder","family":"Roman-Sosa","sequence":"first","affiliation":[{"name":"Department of Internal Medicine I, Ulm University Hospital, 89081 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0803-1306","authenticated-orcid":false,"given":"Anne","family":"Leske","sequence":"additional","affiliation":[{"name":"Laboratory for Arenavirus Biology, Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, 17493 Greifswald, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xenia","family":"Ficht","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Ulm University Hospital, 89081 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tung Huy","family":"Dau","sequence":"additional","affiliation":[{"name":"Laboratory for Immunogenetics, Institute of Immunology, Friedrich-Loeffler-Institut, 17493 Greifswald, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0554-8337","authenticated-orcid":false,"given":"Julia","family":"Holzerland","sequence":"additional","affiliation":[{"name":"Laboratory for Arenavirus Biology, Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, 17493 Greifswald, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5829-6305","authenticated-orcid":false,"given":"Thomas","family":"Hoenen","sequence":"additional","affiliation":[{"name":"Laboratory for Integrative Cell and Infection Biology, Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, 17493 Greifswald, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Beer","sequence":"additional","affiliation":[{"name":"National and OIE Reference Laboratory for BHV-1, Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3038-8205","authenticated-orcid":false,"given":"Robert","family":"Kammerer","sequence":"additional","affiliation":[{"name":"Laboratory for Immunogenetics, Institute of Immunology, Friedrich-Loeffler-Institut, 17493 Greifswald, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhold","family":"Schirmbeck","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Ulm University Hospital, 89081 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9953-7988","authenticated-orcid":false,"given":"Felix A.","family":"Rey","sequence":"additional","affiliation":[{"name":"Structural Virology Unit, CNRS UMR3569, Institut Pasteur, Universit\u00e9 de Paris, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0652-0803","authenticated-orcid":false,"given":"Sandra M.","family":"Cordo","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), University of Buenos Aires, Ciudad Universitaria, Pabell\u00f3n II, Piso 4, Buenos Aires 1428, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9528-5130","authenticated-orcid":false,"given":"Allison","family":"Groseth","sequence":"additional","affiliation":[{"name":"Laboratory for Arenavirus Biology, Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, 17493 Greifswald, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.coviro.2019.06.005","article-title":"A short history of Lassa fever: The first 10\u201315 years after discovery","volume":"37","author":"Monath","year":"2019","journal-title":"Curr. Opin. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1146\/annurev-virology-101416-042001","article-title":"New World Arenavirus Biology","volume":"4","author":"Sarute","year":"2017","journal-title":"Annu. Rev. Virol."},{"key":"ref_3","first-page":"65","article-title":"Human infection with arenaviruses in the Americas","volume":"262","author":"Peters","year":"2002","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"287","DOI":"10.7705\/biomedica.v22i3.1166","article-title":"Viral hemorrhagic fevers of South America","volume":"22","author":"Tesh","year":"2002","journal-title":"Biomedica"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"554","DOI":"10.4269\/ajtmh.1994.51.554","article-title":"Prevalence of infection with Junin virus in rodent populations in the epidemic area of Argentine hemorrhagic fever","volume":"51","author":"Mills","year":"1994","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"694","DOI":"10.4161\/hv.7.6.15198","article-title":"Argentine hemorrhagic fever vaccines","volume":"7","author":"Ambrosio","year":"2011","journal-title":"Hum. Vaccin."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"6045","DOI":"10.1128\/JVI.02392-07","article-title":"Site 1 protease is required for proteolytic processing of the glycoproteins of the South American hemorrhagic fever viruses Junin, Machupo, and Guanarito","volume":"82","author":"Rojek","year":"2008","journal-title":"J. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2162","DOI":"10.3390\/v4102162","article-title":"Envelope glycoprotein of arenaviruses","volume":"4","author":"Burri","year":"2012","journal-title":"Viruses"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4024","DOI":"10.1128\/JVI.06397-11","article-title":"An antibody recognizing the apical domain of human transferrin receptor 1 efficiently inhibits the entry of all new world hemorrhagic Fever arenaviruses","volume":"86","author":"Helguera","year":"2012","journal-title":"J. Virol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1038\/nature05539","article-title":"Transferrin receptor 1 is a cellular receptor for New World haemorrhagic fever arenaviruses","volume":"446","author":"Radoshitzky","year":"2007","journal-title":"Nature"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1038\/nsmb.1772","article-title":"Structural basis for receptor recognition by New World hemorrhagic fever arenaviruses","volume":"17","author":"Abraham","year":"2010","journal-title":"Nat. Struct. Mol. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.chom.2015.11.005","article-title":"Molecular Basis for Antibody-Mediated Neutralization of New World Hemorrhagic Fever Mammarenaviruses","volume":"18","author":"Mahmutovic","year":"2015","journal-title":"Cell Host Microbe"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1038\/s41586-020-2852-1","article-title":"SARS-CoV-2 neutralizing antibody structures inform therapeutic strategies","volume":"588","author":"Barnes","year":"2020","journal-title":"Nature"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"637982","DOI":"10.3389\/fimmu.2021.637982","article-title":"Induction of Potent and Durable Neutralizing Antibodies Against SARS-CoV-2 Using a Receptor Binding Domain-Based Immunogen","volume":"12","author":"Srivastava","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1107\/S0907444906029799","article-title":"A time- and cost-efficient system for high-level protein production in mammalian cells","volume":"62","author":"Aricescu","year":"2006","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1073\/pnas.96.5.1915","article-title":"Native display of complete foreign protein domains on the surface of hepatitis B virus capsids","volume":"96","author":"Kratz","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1099\/jgv.0.000377","article-title":"Analysis of the humoral immune response against the envelope glycoprotein Gc of Schmallenberg virus reveals a domain located at the amino terminus targeted by mAbs with neutralizing activity","volume":"97","author":"Brocchi","year":"2016","journal-title":"J. Gen. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.ymthe.2019.01.011","article-title":"Endogenously Expressed Antigens Bind Mammalian RNA via Cationic Domains that Enhance Priming of Effector CD8 T Cells by DNA Vaccination","volume":"27","author":"Krieger","year":"2019","journal-title":"Mol. Ther."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1038\/s41586-018-0702-1","article-title":"Protocadherin-1 is essential for cell entry by New World hantaviruses","volume":"563","author":"Jangra","year":"2018","journal-title":"Nature"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.antiviral.2018.07.009","article-title":"Lifecycle modelling systems support inosine monophosphate dehydrogenase (IMPDH) as a pro-viral factor and antiviral target for New World arenaviruses","volume":"157","author":"Dunham","year":"2018","journal-title":"Antiviral Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.antiviral.2019.01.006","article-title":"Assessing cross-reactivity of Jun\u00edn virus-directed neutralizing antibodies","volume":"163","author":"Leske","year":"2019","journal-title":"Antiviral Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"20","DOI":"10.3389\/fcimb.2017.00020","article-title":"Absence of an N-Linked Glycosylation Motif in the Glycoprotein of the Live-Attenuated Argentine Hemorrhagic Fever Vaccine, Candid #1, Results in Its Improper Processing, and Reduced Surface Expression","volume":"7","author":"Manning","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1002\/jmv.10308","article-title":"IgG subclasses in human immune response to wild and attenuated (vaccine) Junin virus infection","volume":"69","author":"Sottosanti","year":"2003","journal-title":"J. Med. Virol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0140-6736(84)90299-X","article-title":"Importance of dose of neutralising antibodies in treatment of Argentine haemorrhagic fever with immune plasma","volume":"2","author":"Enria","year":"1984","journal-title":"Lancet"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3515","DOI":"10.1128\/JVI.02969-15","article-title":"Glycoprotein-Specific Antibodies Produced by DNA Vaccination Protect Guinea Pigs from Lethal Argentine and Venezuelan Hemorrhagic Fever","volume":"90","author":"Golden","year":"2016","journal-title":"J. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e2023332118","DOI":"10.1073\/pnas.2023332118","article-title":"Therapy for Argentine hemorrhagic fever in nonhuman primates with a humanized monoclonal antibody","volume":"118","author":"Zeitlin","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"4713","DOI":"10.1016\/j.vaccine.2010.04.077","article-title":"TC83 replicon vectored vaccine provides protection against Junin virus in guinea pigs","volume":"28","author":"Seregin","year":"2010","journal-title":"Vaccine"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Borenstein-Katz, A., Shulman, A., Hamawi, H., Leitner, O., and Diskin, R. (2019). Differential Antibody-Based Immune Response against Isolated GP1 Receptor-Binding Domains from Lassa and Jun\u00edn Viruses. J. Virol., 93.","DOI":"10.1128\/JVI.00090-19"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"104666","DOI":"10.1016\/j.antiviral.2019.104666","article-title":"Development of horse neutralizing immunoglobulin and immunoglobulin fragments against Jun\u00edn virus","volume":"174","author":"Pan","year":"2020","journal-title":"Antiviral Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1016\/j.ijbiomac.2020.07.056","article-title":"Effect of N-terminal poly histidine-tag on immunogenicity of Streptococcus pneumoniae surface protein SP0845","volume":"163","author":"Singh","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1038\/s41541-020-00219-x","article-title":"Adjuvant formulated virus-like particles expressing native-like forms of the Lassa virus envelope surface glycoprotein are immunogenic and induce antibodies with broadly neutralizing activity","volume":"5","author":"Fehling","year":"2020","journal-title":"NPJ Vaccines"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.antiviral.2018.06.002","article-title":"Novel neutralizing monoclonal antibodies against Junin virus","volume":"156","author":"Pan","year":"2018","journal-title":"Antivir. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"7031","DOI":"10.1073\/pnas.1702127114","article-title":"Convergent immunological solutions to Argentine hemorrhagic fever virus neutralization","volume":"114","author":"Zeltina","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1099\/0022-1317-70-5-1125","article-title":"Junin virus monoclonal antibodies: Characterization and cross-reactivity with other arenaviruses","volume":"70","author":"Sanchez","year":"1989","journal-title":"J. Gen. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1884","DOI":"10.1038\/s41467-018-04271-z","article-title":"Vaccine-elicited receptor-binding site antibodies neutralize two New World hemorrhagic fever arenaviruses","volume":"9","author":"Clark","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Makdasi, E., Levy, Y., Alcalay, R., Noy-Porat, T., Zahavy, E., Mechaly, A., Epstein, E., Peretz, E., Cohen, H., and Bar-On, L. (2021). Neutralizing Monoclonal Anti-SARS-CoV-2 Antibodies Isolated from Immunized Rabbits Define Novel Vulnerable Spike-Protein Epitope. Viruses, 13.","DOI":"10.3390\/v13040566"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1080\/19420862.2017.1289302","article-title":"Humanization of rabbit monoclonal antibodies via grafting combined Kabat\/IMGT\/Paratome complementarity-determining regions: Rationale and examples","volume":"9","author":"Zhang","year":"2017","journal-title":"MAbs"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1038\/s41579-019-0233-2","article-title":"Therapeutic strategies to target the Ebola virus life cycle","volume":"17","author":"Hoenen","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"42500","DOI":"10.1038\/srep42500","article-title":"The N-terminal domain of Schmallenberg virus envelope protein Gc is highly immunogenic and can provide protection from infection","volume":"7","author":"Wernike","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"E690","DOI":"10.1073\/pnas.1115485109","article-title":"Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin","volume":"109","author":"Zakeri","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1080\/22221751.2020.1760735","article-title":"Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV","volume":"9","author":"Okba","year":"2020","journal-title":"Emerg. Microbes Infect."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e0186820","DOI":"10.1128\/JVI.01868-20","article-title":"Antibody-Based Inhibition of Pathogenic New World Hemorrhagic Fever Mammarenaviruses by Steric Occlusion of the Human Transferrin Receptor 1 Apical Domain","volume":"95","author":"Ferrero","year":"2021","journal-title":"J. Virol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/s41467-019-13924-6","article-title":"Rational design of universal immunotherapy for TfR1-tropic arenaviruses","volume":"11","author":"Amon","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1006\/biol.2001.0308","article-title":"Antigenicity and immunogenicity of synthetic peptides","volume":"29","year":"2001","journal-title":"Biologicals"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"4204","DOI":"10.3390\/v7082818","article-title":"Evaluation of Trichodysplasia Spinulosa-Associated Polyomavirus Capsid Protein as a New Carrier for Construction of Chimeric Virus-Like Particles Harboring Foreign Epitopes","volume":"7","author":"Gedvilaite","year":"2015","journal-title":"Viruses"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"640","DOI":"10.3390\/v6020640","article-title":"The use of chimeric virus-like particles harbouring a segment of hantavirus Gc glycoprotein to generate a broadly-reactive hantavirus-specific monoclonal antibody","volume":"6","author":"Zvirbliene","year":"2014","journal-title":"Viruses"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"598","DOI":"10.3389\/fimmu.2018.00598","article-title":"Distinct Immunologic Properties of Soluble Versus Particulate Antigens","volume":"9","author":"Snapper","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1126\/science.8248784","article-title":"The influence of antigen organization on B cell responsiveness","volume":"262","author":"Bachmann","year":"1993","journal-title":"Science"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.pep.2010.09.010","article-title":"Highly efficient production of phosphorylated hepatitis B core particles in yeast Pichia pastoris","volume":"75","author":"Freivalds","year":"2011","journal-title":"Protein Expr. Purif."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/S1097-2765(01)80009-5","article-title":"The crystal structure of the human hepatitis B virus capsid","volume":"3","author":"Wynne","year":"1999","journal-title":"Mol. Cell"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"9630","DOI":"10.1038\/s41598-017-08787-0","article-title":"Cell-permeable capsids as universal antigen carrier for the induction of an antigen-specific CD8+ T-cell response","volume":"7","author":"Akhras","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0065-3527(08)60808-8","article-title":"Core particles of hepatitis B virus as carrier for foreign epitopes","volume":"50","author":"Ulrich","year":"1998","journal-title":"Adv. Virus Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1128\/jvi.66.1.106-114.1992","article-title":"The position of heterologous epitopes inserted in hepatitis B virus core particles determines their immunogenicity","volume":"66","author":"Moriarty","year":"1992","journal-title":"J. Virol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1586\/erv.09.121","article-title":"Use of hepadnavirus core proteins as vaccine platforms","volume":"8","author":"Whitacre","year":"2009","journal-title":"Expert Rev. Vaccines"}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/10\/2\/173\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:06:02Z","timestamp":1760133962000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/10\/2\/173"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,22]]},"references-count":54,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["vaccines10020173"],"URL":"https:\/\/doi.org\/10.3390\/vaccines10020173","relation":{},"ISSN":["2076-393X"],"issn-type":[{"value":"2076-393X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,22]]}}}