{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:30:43Z","timestamp":1789587043860,"version":"build-2803163510"},"reference-count":40,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T00:00:00Z","timestamp":1651017600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["NRCT5-RSA63004"],"award-info":[{"award-number":["NRCT5-RSA63004"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["PHD\/0221\/2561"],"award-info":[{"award-number":["PHD\/0221\/2561"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["BBS\/E\/I\/00007039"],"award-info":[{"award-number":["BBS\/E\/I\/00007039"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Royal Golden Jubilee PhD-Program","award":["NRCT5-RSA63004"],"award-info":[{"award-number":["NRCT5-RSA63004"]}]},{"name":"Royal Golden Jubilee PhD-Program","award":["PHD\/0221\/2561"],"award-info":[{"award-number":["PHD\/0221\/2561"]}]},{"name":"Royal Golden Jubilee PhD-Program","award":["BBS\/E\/I\/00007039"],"award-info":[{"award-number":["BBS\/E\/I\/00007039"]}]},{"name":"Biotechnology and Biological Sciences Research Council (BBSRC)","award":["NRCT5-RSA63004"],"award-info":[{"award-number":["NRCT5-RSA63004"]}]},{"name":"Biotechnology and Biological Sciences Research Council (BBSRC)","award":["PHD\/0221\/2561"],"award-info":[{"award-number":["PHD\/0221\/2561"]}]},{"name":"Biotechnology and Biological Sciences Research Council (BBSRC)","award":["BBS\/E\/I\/00007039"],"award-info":[{"award-number":["BBS\/E\/I\/00007039"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Duck enteritis virus (DEV) and Pasteurella multocida, the causative agent of duck plague and fowl cholera, are acute contagious diseases and leading causes of morbidity and mortality in duck. The NHEJ-CRISPR\/Cas9-mediated gene editing strategy, accompanied with the Cre\u2013Lox system, have been employed in the present study to show that two new sites at UL55-LORF11 and UL44-44.5 loci in the genome of the attenuated Jansen strain of DEV can be used for the stable expression of the outer membrane protein H (ompH) gene of P. multocida that could be used as a bivalent vaccine candidate with the potential of protecting ducks simultaneously against major viral and bacterial pathogens. The two recombinant viruses, DEV-OmpH-V5-UL55-LORF11 and DEV-OmpH-V5-UL44-44.5, with the insertion of ompH-V5 gene at the UL55-LORF11 and UL44-44.5 loci respectively, showed similar growth kinetics and plaque size, compared to the wildtype virus, confirming that the insertion of the foreign gene into these did not have any detrimental effects on DEV. This is the first time the CRISPR\/Cas9 system has been applied to insert a highly immunogenic gene from bacteria into the DEV genome rapidly and efficiently. This approach offers an efficient way to introduce other antigens into the DEV genome for multivalent vector.<\/jats:p>","DOI":"10.3390\/vaccines10050686","type":"journal-article","created":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T22:20:20Z","timestamp":1651098020000},"page":"686","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["CRISPR\/Cas9 Editing of Duck Enteritis Virus Genome for the Construction of a Recombinant Vaccine Vector Expressing ompH Gene of Pasteurella multocida in Two Novel Insertion Sites"],"prefix":"10.3390","volume":"10","author":[{"given":"Nisachon","family":"Apinda","sequence":"first","affiliation":[{"name":"Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxiu","family":"Yao","sequence":"additional","affiliation":[{"name":"The Pirbright Institute, Ash Road, Woking GU24 0NF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoyao","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Pirbright Institute, Ash Road, Woking GU24 0NF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishwanatha R. A. P.","family":"Reddy","sequence":"additional","affiliation":[{"name":"The Pirbright Institute, Ash Road, Woking GU24 0NF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengxiang","family":"Chang","sequence":"additional","affiliation":[{"name":"The Pirbright Institute, Ash Road, Woking GU24 0NF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8255-516X","authenticated-orcid":false,"given":"Venugopal","family":"Nair","sequence":"additional","affiliation":[{"name":"The Pirbright Institute, Ash Road, Woking GU24 0NF, UK"},{"name":"Jenner Institute, University of Oxford, Oxford OX1 2JD, UK"},{"name":"Department of Zoology, University of Oxford, Oxford OX1 2JD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2329-8802","authenticated-orcid":false,"given":"Nattawooti","family":"Sthitmatee","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50200, Thailand"},{"name":"Excellence Center in Veterinary Bioscience, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,27]]},"reference":[{"key":"ref_1","unstructured":"Boulianne, M., and American Association of Avian Pathologists (2013). 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