{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T21:28:17Z","timestamp":1782422897447,"version":"3.54.5"},"reference-count":81,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T00:00:00Z","timestamp":1656892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Division of Worldwide Research and Development, Pfizer, Inc."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Human cytomegalovirus (HCMV) is the leading viral cause of congenital disease and permanent birth defects worldwide. Although the development of an effective vaccine is a public health priority, no vaccines are approved. Among the major antigenic targets are glycoproteins in the virion envelope, including gB, which facilitates cellular entry, and the pentameric complex (gH\/gL\/pUL128-131), required for the infection of specialized cell types. In this study, sera from rabbits immunized with the recombinant pentameric complex were tested for their ability to neutralize infection of epithelial cells, fibroblasts, and primary placental cell types. Sera from rhesus macaques immunized with recombinant gB or gB plus pentameric complex were tested for HCMV neutralizing activity on both cultured cells and cell column cytotrophoblasts in first-trimester chorionic villus explants. Sera from rabbits immunized with the pentameric complex potently blocked infection by pathogenic viral strains in amniotic epithelial cells and cytotrophoblasts but were less effective in fibroblasts and trophoblast progenitor cells. Sera from rhesus macaques immunized with the pentameric complex and gB more strongly reduced infection in fibroblasts, epithelial cells, and chorionic villus explants than sera from immunization with gB alone. These results suggest that the pentameric complex and gB together elicit antibodies that could have potential as prophylactic vaccine antigens.<\/jats:p>","DOI":"10.3390\/vaccines10071074","type":"journal-article","created":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T23:38:55Z","timestamp":1656977935000},"page":"1074","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Neutralizing Antibodies to Human Cytomegalovirus Recombinant Proteins Reduce Infection in an Ex Vivo Model of Developing Human Placentas"],"prefix":"10.3390","volume":"10","author":[{"given":"Takako","family":"Tabata","sequence":"first","affiliation":[{"name":"Cell and Tissue Biology, School of Dentistry, University of California, San Francisco, CA 94143, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Petitt","sequence":"additional","affiliation":[{"name":"Cell and Tissue Biology, School of Dentistry, University of California, San Francisco, CA 94143, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Li","sequence":"additional","affiliation":[{"name":"Vaccine Research and Development, Pfizer, Inc., New York, NY 10965, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyuan","family":"Chi","sequence":"additional","affiliation":[{"name":"Vaccine Research and Development, Pfizer, Inc., New York, NY 10965, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"Vaccine Research and Development, Pfizer, Inc., New York, NY 10965, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Yurgelonis","sequence":"additional","affiliation":[{"name":"Vaccine Research and Development, Pfizer, Inc., New York, NY 10965, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Wellnitz","sequence":"additional","affiliation":[{"name":"Vaccine Research and Development, Pfizer, Inc., New York, NY 10965, USA"},{"name":"RedVax Inc., a Wholly Owned Subsidiary of Pfizer, Inc., 8001 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Bredl","sequence":"additional","affiliation":[{"name":"RedVax Inc., a Wholly Owned Subsidiary of Pfizer, Inc., 8001 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago","family":"Vicente","sequence":"additional","affiliation":[{"name":"RedVax Inc., a Wholly Owned Subsidiary of Pfizer, Inc., 8001 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinzhen","family":"Yang","sequence":"additional","affiliation":[{"name":"Vaccine Research and Development, Pfizer, Inc., New York, NY 10965, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip R.","family":"Dormitzer","sequence":"additional","affiliation":[{"name":"Vaccine Research and Development, Pfizer, Inc., New York, NY 10965, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenore","family":"Pereira","sequence":"additional","affiliation":[{"name":"Cell and Tissue Biology, School of Dentistry, University of California, San Francisco, CA 94143, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.jcv.2011.07.005","article-title":"Intrauterine transmission and clinical outcome of 248 pregnancies with primary cytomegalovirus infection in relation to gestational age","volume":"52","author":"Enders","year":"2011","journal-title":"J. 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