{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T04:15:34Z","timestamp":1777954534744,"version":"3.51.4"},"reference-count":45,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T00:00:00Z","timestamp":1660262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Program of Guangzhou, China","award":["202206010161"],"award-info":[{"award-number":["202206010161"]}]},{"name":"Science and Technology Program of Guangzhou, China","award":["2019KZDXM026"],"award-info":[{"award-number":["2019KZDXM026"]}]},{"name":"Science and Technology Program of Guangzhou, China","award":["2020B0301030007"],"award-info":[{"award-number":["2020B0301030007"]}]},{"name":"Key Research Projects of Universities in Guangdong Province","award":["202206010161"],"award-info":[{"award-number":["202206010161"]}]},{"name":"Key Research Projects of Universities in Guangdong Province","award":["2019KZDXM026"],"award-info":[{"award-number":["2019KZDXM026"]}]},{"name":"Key Research Projects of Universities in Guangdong Province","award":["2020B0301030007"],"award-info":[{"award-number":["2020B0301030007"]}]},{"name":"Guangdong Major Project of Basic and Applied Basic Research","award":["202206010161"],"award-info":[{"award-number":["202206010161"]}]},{"name":"Guangdong Major Project of Basic and Applied Basic Research","award":["2019KZDXM026"],"award-info":[{"award-number":["2019KZDXM026"]}]},{"name":"Guangdong Major Project of Basic and Applied Basic Research","award":["2020B0301030007"],"award-info":[{"award-number":["2020B0301030007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Japanese encephalitis is a mosquito-borne zoonotic epidemic caused by the Japanese encephalitis virus (JEV). JEV is not only the leading cause of Asian viral encephalitis, but also one of the leading causes of viral encephalitis worldwide. To understand the genetic evolution and E protein characteristics of JEV, 263 suspected porcine JE samples collected from South China from 2011 to 2018 were inspected. It was found that 78 aborted porcine fetuses were JEV-nucleic-acid-positive, with a positive rate of 29.7%. Furthermore, four JEV variants were isolated from JEV-nucleic-acid-positive materials, namely, CH\/GD2011\/2011, CH\/GD2014\/2014, CH\/GD2015\/2015, and CH\/GD2018\/2018. The cell culture and virus titer determination of four JEV isolates showed that four JEV isolates could proliferate stably in Vero cells, and the virus titer was as high as 108.5 TCID 50\/mL. The whole-genome sequences of four JEV isolates were sequenced. Based on the phylogenetic analysis of the JEV E gene and whole genome, it was found that CH\/GD2011\/2011 and CH\/GD2015\/2015 belonged to the GIII type, while CH\/GD2014\/2014 and CH\/GD2018\/2018 belonged to the GI type, which was significantly different from that of the JEV classical strain CH\/BJ-1\/1995. Bioinformatics tools were used to analyze the E protein phosphorylation site, glycosylation site, B cell antigen epitope, and modeled 3D structures of E protein in four JEV isolates. The analysis of the prevalence of JEV and the biological function of E protein can provide a theoretical basis for the prevention and control of JEV and the design of antiviral drugs.<\/jats:p>","DOI":"10.3390\/vaccines10081303","type":"journal-article","created":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T01:51:11Z","timestamp":1660269071000},"page":"1303","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Genomic Characteristics and E Protein Bioinformatics Analysis of JEV Isolates from South China from 2011 to 2018"],"prefix":"10.3390","volume":"10","author":[{"given":"Yawei","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"},{"name":"Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongxing","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"},{"name":"Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feifan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"},{"name":"Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7920-4115","authenticated-orcid":false,"given":"Quanhui","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"},{"name":"Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuwan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinni","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weijun","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keke","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Ling","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuangqi","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"},{"name":"Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingqiu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"},{"name":"Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"},{"name":"Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinding","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"},{"name":"Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3091","DOI":"10.1128\/JVI.77.5.3091-3098.2003","article-title":"Origin and evolution of Japanese encephalitis virus in southeast Asia","volume":"77","author":"Solomon","year":"2003","journal-title":"J. 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