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The objective of next-generation influenza vaccines is not only to increase the breadth of response but also to improve the elicitation of an effective and robust immune response, especially in high-risk populations. To achieve this second objective, the administration of adjuvanted influenza vaccines has been considered. In this regard, the monitoring and characterization of the antibody response associated with the administration of adjuvanted vaccines has been evaluated in this study in order to shed light on the kinetic, magnitude and subclass usage of antibody secreting cells (ASCs) as well as of circulating antigen-specific serum antibodies. Specifically, we utilized the DBA\/2J mouse model to assess the kinetic, magnitude and IgG subclass usage of the antibody response following an intramuscular (IM) or intraperitoneal (IP) immunization regimen with AddaVax-adjuvanted bivalent H1N1 and H3N2 computationally optimized broadly reactive antigen (COBRA) influenza recombinant hemagglutinins (rHAs). While the serological evaluation revealed a homogeneous kinetic of the antibody response, the detection of the ASCs through a FluoroSpot platform revealed a different magnitude, subclass usage and kinetic of the antigen-specific IgG secreting cells peaking at day 5 and day 9 following the IP and IM immunization, respectively.<\/jats:p>","DOI":"10.3390\/vaccines10081315","type":"journal-article","created":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T03:40:32Z","timestamp":1660621232000},"page":"1315","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Kinetic of the Antibody Response Following AddaVax-Adjuvanted Immunization with Recombinant Influenza Antigens"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1947-7469","authenticated-orcid":false,"given":"Ted. M.","family":"Ross","sequence":"first","affiliation":[{"name":"Center for Vaccines and Immunology, University of Georgia, Athens, GA 30602, USA"},{"name":"Department of Infectious Diseases, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naveen","family":"Gokanapudi","sequence":"additional","affiliation":[{"name":"Center for Vaccines and Immunology, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Ge","sequence":"additional","affiliation":[{"name":"Center for Vaccines and Immunology, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Shi","sequence":"additional","affiliation":[{"name":"Center for Vaccines and Immunology, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Richardson","sequence":"additional","affiliation":[{"name":"Center for Vaccines and Immunology, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8913-6657","authenticated-orcid":false,"given":"Spencer R.","family":"Pierce","sequence":"additional","affiliation":[{"name":"Center for Vaccines and Immunology, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Center for Vaccines and Immunology, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2105-0117","authenticated-orcid":false,"given":"Subhan","family":"Ullah","sequence":"additional","affiliation":[{"name":"Center for Vaccines and Immunology, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana","family":"De Luca","sequence":"additional","affiliation":[{"name":"Center for Vaccines and Immunology, University of Georgia, Athens, GA 30602, USA"},{"name":"Department of Infectious Diseases, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe A.","family":"Sautto","sequence":"additional","affiliation":[{"name":"Center for Vaccines and Immunology, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,14]]},"reference":[{"key":"ref_1","unstructured":"WHO (2022, August 13). Influenza (Seasonal). Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/influenza-(seasonal)."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Guthmiller, J.J., Utset, H.A., and Wilson, P.C. (2021). B Cell Responses against Influenza Viruses: Short-Lived Humoral Immunity against a Life-Long Threat. Viruses, 13.","DOI":"10.3390\/v13060965"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"408","DOI":"10.3201\/eid1503.080238","article-title":"Prevalence and seasonality of influenza-like illness in children, Nicaragua, 2005\u20132007","volume":"15","author":"Gordon","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1080\/21645515.2017.1343226","article-title":"Influenza vaccination in the elderly","volume":"14","author":"Smetana","year":"2018","journal-title":"Hum. Vaccin. Immunother."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/S1473-3099(09)70175-6","article-title":"Influenza in immunosuppressed populations: A review of infection frequency, morbidity, mortality, and vaccine responses","volume":"9","author":"Kunisaki","year":"2009","journal-title":"Lancet Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Abreu, R.B., Kirchenbaum, G.A., Sautto, G.A., Clutter, E.F., and Ross, T.M. (2021). Impaired memory B-cell recall responses in the elderly following recurrent influenza vaccination. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0254421"},{"key":"ref_7","unstructured":"CDC (2022, August 13). Vaccine Effectiveness: How Well Do Flu Vaccines Work?, Available online: https:\/\/www.cdc.gov\/flu\/vaccines-work\/vaccineeffect.htm."},{"key":"ref_8","unstructured":"(2022, August 13). CDC, Available online: https:\/\/www.cdc.gov\/flu\/prevent\/adjuvant.htm."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"902","DOI":"10.3389\/fimmu.2020.00902","article-title":"IgA Responses Following Recurrent Influenza Virus Vaccination","volume":"11","author":"Abreu","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"12825","DOI":"10.1128\/JVI.05930-11","article-title":"The DBA.2 mouse is susceptible to disease following infection with a broad, but limited, range of influenza A and B viruses","volume":"85","author":"Pica","year":"2011","journal-title":"J. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1186\/1743-422X-9-212","article-title":"Immunization with live virus vaccine protects highly susceptible DBA\/2J mice from lethal influenza A H1N1 infection","volume":"9","author":"Dengler","year":"2012","journal-title":"Virol. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e0165221","DOI":"10.1128\/jvi.01652-21","article-title":"Bivalent H1 and H3 COBRA Recombinant Hemagglutinin Vaccines Elicit Seroprotective Antibodies against H1N1 and H3N2 Influenza Viruses from 2009 to 2019","volume":"96","author":"Allen","year":"2022","journal-title":"J. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"226","DOI":"10.4049\/immunohorizons.1800044","article-title":"Elicitation of Broadly Protective Antibodies following Infection with Influenza Viruses Expressing H1N1 Computationally Optimized Broadly Reactive Hemagglutinin Antigens","volume":"2","author":"Sautto","year":"2018","journal-title":"Immunohorizons"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"375","DOI":"10.4049\/jimmunol.1900379","article-title":"A Computationally Optimized Broadly Reactive Antigen Subtype-Specific Influenza Vaccine Strategy Elicits Unique Potent Broadly Neutralizing Antibodies against Hemagglutinin","volume":"204","author":"Sautto","year":"2020","journal-title":"J. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Forgacs, D., Silva-Moraes, V., Sautto, G.A., Hanley, H.B., Gattiker, J.L., Jefferson, A.M., Kolhe, R., and Ross, T.M. (2022). The Effect of Waning on Antibody Levels and Memory B Cell Recall following SARS-CoV-2 Infection or Vaccination. Vaccines, 10.","DOI":"10.1101\/2022.03.16.484099"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1172\/JCI106279","article-title":"Metabolic properties of IgG subclasses in man","volume":"49","author":"Morell","year":"1970","journal-title":"J. Clin. Investig."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1002\/eji.1830180221","article-title":"The half-lives of serum immunoglobulins in adult mice","volume":"18","author":"Vieira","year":"1988","journal-title":"Eur. J. Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2911","DOI":"10.1128\/JVI.06075-11","article-title":"Rapid and massive virus-specific plasmablast responses during acute dengue virus infection in humans","volume":"86","author":"Wrammert","year":"2012","journal-title":"J. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1038\/nature06890","article-title":"Rapid cloning of high-affinity human monoclonal antibodies against influenza virus","volume":"453","author":"Wrammert","year":"2008","journal-title":"Nature"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1146\/annurev.immunol.23.021704.115723","article-title":"Maintenance of serum antibody levels","volume":"23","author":"Manz","year":"2005","journal-title":"Annu. Rev. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1084\/jem.191.3.475","article-title":"bcl-2 transgene expression inhibits apoptosis in the germinal center and reveals differences in the selection of memory B cells and bone marrow antibody-forming cells","volume":"191","author":"Smith","year":"2000","journal-title":"J. Exp. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2419","DOI":"10.1084\/jem.20061254","article-title":"High affinity germinal center B cells are actively selected into the plasma cell compartment","volume":"203","author":"Phan","year":"2006","journal-title":"J. Exp. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"965","DOI":"10.3389\/fimmu.2019.00965","article-title":"Survival of Long-Lived Plasma Cells (LLPC): Piecing Together the Puzzle","volume":"10","author":"Lightman","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1038\/nri1572","article-title":"Regulation of plasma-cell development","volume":"5","author":"Calame","year":"2005","journal-title":"Nat. Rev. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1126\/science.aaz8432","article-title":"Influenza vaccine-induced human bone marrow plasma cells decline within a year after vaccination","volume":"370","author":"Davis","year":"2020","journal-title":"Science"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1016\/j.cell.2019.04.036","article-title":"Longitudinal Analysis of the Human B Cell Response to Ebola Virus Infection","volume":"177","author":"Davis","year":"2019","journal-title":"Cell"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.ebiom.2019.02.060","article-title":"Longitudinal analysis of acute and convalescent B cell responses in a human primary dengue serotype 2 infection model","volume":"41","author":"Nivarthi","year":"2019","journal-title":"eBioMedicine"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/s41586-021-03647-4","article-title":"SARS-CoV-2 infection induces long-lived bone marrow plasma cells in humans","volume":"595","author":"Turner","year":"2021","journal-title":"Nature"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Huang, Y., Franca, M.S., Allen, J.D., Shi, H., and Ross, T.M. (2021). Next Generation of Computationally Optimized Broadly Reactive HA Vaccines Elicited Cross-Reactive Immune Responses and Provided Protection against H1N1 Virus Infection. Vaccines, 9.","DOI":"10.3390\/vaccines9070793"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Ecker, J.W., Kirchenbaum, G.A., Pierce, S.R., Skarlupka, A.L., Abreu, R.B., Cooper, R.E., Taylor-Mulneix, D., Ross, T.M., and Sautto, G.A. (2020). High-Yield Expression and Purification of Recombinant Influenza Virus Proteins from Stably-Transfected Mammalian Cell Lines. Vaccines, 8.","DOI":"10.3390\/vaccines8030462"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1038\/s41541-021-00418-0","article-title":"\u201cWorld in motion\u201d\u2014Emulsion adjuvants rising to meet the pandemic challenges","volume":"6","author":"Lodaya","year":"2021","journal-title":"NPJ Vaccines"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3363","DOI":"10.1016\/j.vaccine.2013.05.007","article-title":"The adjuvant effect of MF59 is due to the oil-in-water emulsion formulation, none of the individual components induce a comparable adjuvant effect","volume":"31","author":"Calabro","year":"2013","journal-title":"Vaccine"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.4049\/jimmunol.1400623","article-title":"Vaccine adjuvant MF59 promotes retention of unprocessed antigen in lymph node macrophage compartments and follicular dendritic cells","volume":"194","author":"Cantisani","year":"2015","journal-title":"J. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e74","DOI":"10.1038\/cti.2016.19","article-title":"Local innate immune responses in the vaccine adjuvant-injected muscle","volume":"5","author":"Liang","year":"2016","journal-title":"Clin. Transl. Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"37229","DOI":"10.1038\/srep37229","article-title":"Investigating the effect of AS03 adjuvant on the plasma cell repertoire following pH1N1 influenza vaccination","volume":"6","author":"Galson","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1038\/s41541-018-0076-2","article-title":"AS03-adjuvanted H5N1 vaccine promotes antibody diversity and affinity maturation, NAI titers, cross-clade H5N1 neutralization, but not H1N1 cross-subtype neutralization","volume":"3","author":"Khurana","year":"2018","journal-title":"NPJ Vaccines"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1038\/s41591-020-1118-7","article-title":"A chimeric hemagglutinin-based universal influenza virus vaccine approach induces broad and long-lasting immunity in a randomized, placebo-controlled phase I trial","volume":"27","author":"Nachbagauer","year":"2021","journal-title":"Nat. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/s41541-021-00308-5","article-title":"Persistently high antibody responses after AS03-adjuvanted H1N1pdm09 vaccine: Dissecting the HA specific antibody response","volume":"6","author":"Madsen","year":"2021","journal-title":"NPJ Vaccines"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3076","DOI":"10.1016\/j.vaccine.2010.02.050","article-title":"AF03-adjuvanted and non-adjuvanted pandemic influenza A (H1N1) 2009 vaccines induce strong antibody responses in seasonal influenza vaccine-primed and unprimed mice","volume":"28","author":"Caillet","year":"2010","journal-title":"Vaccine"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5373","DOI":"10.1016\/j.vaccine.2012.06.021","article-title":"Advax, a polysaccharide adjuvant derived from delta inulin, provides improved influenza vaccine protection through broad-based enhancement of adaptive immune responses","volume":"30","author":"Saade","year":"2012","journal-title":"Vaccine"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1016\/j.vaccine.2022.01.034","article-title":"A phase 1 dose-sparing, randomized clinical trial of seasonal trivalent inactivated influenza vaccine combined with MAS-1, a novel water-in-oil adjuvant\/delivery system","volume":"40","author":"Gorse","year":"2022","journal-title":"Vaccine"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1007\/s12275-013-3428-7","article-title":"DBA\/2 mouse as an animal model for anti-influenza drug efficacy evaluation","volume":"51","author":"Kim","year":"2013","journal-title":"J. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4720","DOI":"10.1128\/JVI.03152-15","article-title":"Design and Characterization of a Computationally Optimized Broadly Reactive Hemagglutinin Vaccine for H1N1 Influenza Viruses","volume":"90","author":"Carter","year":"2016","journal-title":"J. Virol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3043","DOI":"10.1016\/j.vaccine.2011.01.100","article-title":"A computationally optimized broadly reactive antigen (COBRA) based H5N1 VLP vaccine elicits broadly reactive antibodies in mice and ferrets","volume":"29","author":"Giles","year":"2011","journal-title":"Vaccine"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.1093\/infdis\/jis232","article-title":"A computationally optimized hemagglutinin virus-like particle vaccine elicits broadly reactive antibodies that protect nonhuman primates from H5N1 infection","volume":"205","author":"Giles","year":"2012","journal-title":"J. Infect. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1186\/s13578-021-00656-8","article-title":"Differing susceptibility of C57BL\/6J and DBA\/2J mice-parents of the murine BXD family, to severe acute respiratory syndrome coronavirus infection","volume":"11","author":"Li","year":"2021","journal-title":"Cell Biosci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"13011","DOI":"10.1038\/s41598-017-13331-1","article-title":"Combined skin and muscle vaccination differentially impact the quality of effector T cell functions: The CUTHIVAC-001 randomized trial","volume":"7","author":"Haidari","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e132155","DOI":"10.1172\/jci.insight.132155","article-title":"Preexisting subtype immunodominance shapes memory B cell recall response to influenza vaccination","volume":"5","author":"Abreu","year":"2020","journal-title":"JCI Insight"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"950","DOI":"10.4161\/hv.24939","article-title":"Low immunogenicity predicted for emerging avian-origin H7N9: Implication for influenza vaccine design","volume":"9","author":"Ardito","year":"2013","journal-title":"Hum. Vaccin. Immunother."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"12578","DOI":"10.1073\/pnas.1712377114","article-title":"Contemporary H3N2 influenza viruses have a glycosylation site that alters binding of antibodies elicited by egg-adapted vaccine strains","volume":"114","author":"Zost","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1038\/ni.3533","article-title":"Defining antigen-specific plasmablast and memory B cell subsets in human blood after viral infection or vaccination","volume":"17","author":"Ellebedy","year":"2016","journal-title":"Nat. Immunol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Forgacs, D., Abreu, R.B., Sautto, G.A., Kirchenbaum, G.A., Drabek, E., Williamson, K.S., Kim, D., Emerling, D.E., and Ross, T.M. (2021). Convergent antibody evolution and clonotype expansion following influenza virus vaccination. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0247253"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.addr.2020.06.019","article-title":"Controlling timing and location in vaccines","volume":"158","author":"Irvine","year":"2020","journal-title":"Adv. Drug. Deliv. Rev."},{"key":"ref_54","unstructured":"Academic Press, Kolhe, P., and Ohtake, S. (2022). Conventional and nontraditional delivery methods and routes of vaccine administration. Practical Aspects of Vaccine Development, Elsevier."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.yrtph.2016.08.003","article-title":"Pharmacokinetics and biodistribution of squalene-containing emulsion adjuvant following intramuscular injection of H5N1 influenza vaccine in mice","volume":"81","author":"Tegenge","year":"2016","journal-title":"Regul. Toxicol. Pharmacol."}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/10\/8\/1315\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:08:35Z","timestamp":1760141315000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/10\/8\/1315"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,14]]},"references-count":55,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["vaccines10081315"],"URL":"https:\/\/doi.org\/10.3390\/vaccines10081315","relation":{},"ISSN":["2076-393X"],"issn-type":[{"value":"2076-393X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,14]]}}}