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scholarship","doi-asserted-by":"publisher","award":["2018\/23579-8"],"award-info":[{"award-number":["2018\/23579-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>The apicomplexan parasite Theileria parva is the causative agent of East Coast fever, usually a fatal disease for cattle, which is prevalent in large areas of eastern, central, and southern Africa. Protective immunity against T. parva is mediated by CD8+ T cells, with CD4+ T-cells thought to be important in facilitating the full maturation and development of the CD8+ T-cell response. T. parva has a large proteome, with &gt;4000 protein-coding genes, making T-cell antigen identification using conventional screening approaches laborious and expensive. To date, only a limited number of T-cell antigens have been described. Novel approaches for identifying candidate antigens for T. parva are required to replace and\/or complement those currently employed. In this study, we report on the use of immunopeptidomics to study the repertoire of T. parva peptides presented by both BoLA-I and BoLA-DR molecules on infected cells. The study reports on peptides identified from the analysis of 13 BoLA-I and 6 BoLA-DR datasets covering a range of different BoLA genotypes. This represents the most comprehensive immunopeptidomic dataset available for any eukaryotic pathogen to date. Examination of the immunopeptidome data suggested the presence of a large number of coprecipitated and non-MHC-binding peptides. As part of the work, a pipeline to curate the datasets to remove these peptides was developed and used to generate a final list of 74 BoLA-I and 15 BoLA-DR-presented peptides. Together, the data demonstrated the utility of immunopeptidomics as a method to identify novel T-cell antigens for T. parva and the importance of careful curation and the application of high-quality immunoinformatics to parse the data generated.<\/jats:p>","DOI":"10.3390\/vaccines10111907","type":"journal-article","created":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T02:33:10Z","timestamp":1668393190000},"page":"1907","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Immunopeptidomic Analysis of BoLA-I and BoLA-DR Presented Peptides from Theileria parva Infected Cells"],"prefix":"10.3390","volume":"10","author":[{"given":"Timothy","family":"Connelley","sequence":"first","affiliation":[{"name":"The Roslin Institute, The Royal (Dick) School of Veterinary Science, The University of Edinburgh, Edinburgh EH25 9RG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annalisa","family":"Nicastri","sequence":"additional","affiliation":[{"name":"The Jenner Institute, Nuffield Department of Medicine, The University of Oxford, Oxford OX3 7BN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara","family":"Sheldrake","sequence":"additional","affiliation":[{"name":"The Roslin Institute, The Royal (Dick) School of Veterinary Science, The University of Edinburgh, Edinburgh EH25 9RG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Vrettou","sequence":"additional","affiliation":[{"name":"The Roslin Institute, The Royal (Dick) School of Veterinary Science, The University of Edinburgh, Edinburgh EH25 9RG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8380-6565","authenticated-orcid":false,"given":"Andressa","family":"Fisch","sequence":"additional","affiliation":[{"name":"Ribeir\u00e3o Preto College of Nursing, University of S\u00e3o Paulo, Av Bandeirantes, Ribeir\u00e3o Preto 3900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birkir","family":"Reynisson","sequence":"additional","affiliation":[{"name":"Department of Health Technology, Technical University of Denmark, DK-2800 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soren","family":"Buus","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Immunology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, DK-2200 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Hill","sequence":"additional","affiliation":[{"name":"The Jenner Institute, Nuffield Department of Medicine, The University of Oxford, Oxford OX3 7BN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Morrison","sequence":"additional","affiliation":[{"name":"The Roslin Institute, The Royal (Dick) School of Veterinary Science, The University of Edinburgh, Edinburgh EH25 9RG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7885-4311","authenticated-orcid":false,"given":"Morten","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Health Technology, Technical University of Denmark, DK-2800 Copenhagen, Denmark"},{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas, Universidad Nacional de San Mart\u00edn, San Mart\u00edn CP1650, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Ternette","sequence":"additional","affiliation":[{"name":"The Jenner Institute, Nuffield Department of Medicine, The University of Oxford, Oxford OX3 7BN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,11]]},"reference":[{"key":"ref_1","unstructured":"(2022, September 30). 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