{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T02:06:05Z","timestamp":1769825165388,"version":"3.49.0"},"reference-count":33,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T00:00:00Z","timestamp":1669161600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Subacute thyroiditis (SAT) is a thyroid disease associated with viral infections. Its relationship with major histocompatibility complex (MHC) antigens was shown before. SAT cases triggered by different types of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines have been reported. In this study, human leukocyte antigen (HLA) genotypes of 27 SAT patients (13 vaccine-associated (V-SAT) and 14 non-SARS-CoV-2-infection non-vaccine-associated (non-V-SAT)) were compared with those of 362 healthy donors. HLA analyses were performed with low-resolution DNA-based sequence-specific oligonucleotide or sequence-specific primer methods. Statistical analyses were performed using IBM SPSS Statistics 25 and Stata\/MP 14.1 with the hapipf function. Allele and haplotype frequencies were estimated by PyPop and gene[RATE] tool programs. The allele frequencies of HLA-A*11, HLA-B*35, and HLA-C*04 were higher in the patient groups. Both the allele frequency of HLA-A*11 and the haplotype frequency of A*11-B*35-C*04 were higher in the V-SAT group. The A*11-B*35-C*04 haplotype, including all three loci of MHC class I genes, is shown to be associated with the disease for the first time, especially in the V-SAT group. This finding will contribute to a better understanding of the etiopathogenesis of vaccine-associated SAT and the role of HLA genotypes in the functioning mechanisms of the SARS-CoV-2 vaccines.<\/jats:p>","DOI":"10.3390\/vaccines10121986","type":"journal-article","created":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T03:48:12Z","timestamp":1669175292000},"page":"1986","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A Novel Finding of an HLA Allele\u2019s and a Haplotype\u2019s Relationship with SARS-CoV-2 Vaccine-Associated Subacute Thyroiditis"],"prefix":"10.3390","volume":"10","author":[{"given":"Melisa","family":"Sahin Tekin","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, Eskisehir Osmangazi University, 26040 Eskisehir, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goknur","family":"Yorulmaz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Endocrinology and Metabolism, Faculty of Medicine, Eskisehir Osmangazi University, 26040 Eskisehir, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emel","family":"Yantir","sequence":"additional","affiliation":[{"name":"Department of Immunology, Faculty of Medicine, Eskisehir Osmangazi University, 26040 Eskisehir, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eren","family":"Gunduz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Hematology, Faculty of Medicine, Eskisehir Osmangazi University, 26040 Eskisehir, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ertugrul","family":"Colak","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Faculty of Medicine, Eskisehir Osmangazi University, 26040 Eskisehir, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,23]]},"reference":[{"key":"ref_1","unstructured":"Jameson, J.L., and De Groot, L.J. (2016). Subacute and Riedel\u2019s thyroiditis. Endocrinology Adult and Pediatric, Elsevier Saunders. [7th ed.]."},{"key":"ref_2","unstructured":"Post, T.W. (2022). Subacute thyroiditis. UpToDate, UpToDate. Available online: https:\/\/www.uptodate.com\/contents\/subacute-thyroiditis?search=subacute%20thyroiditis&source=search_result&selectedTitle=1~42&usage_type=default&display_rank=1."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1080\/21645515.2015.1117716","article-title":"Subacute thyroiditis following seasonal influenza vaccination","volume":"12","author":"Altay","year":"2016","journal-title":"Hum. Vaccin Immunother."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1007\/BF03346633","article-title":"Subacute thyroiditis following the H1N1 vaccine","volume":"33","author":"Girgis","year":"2010","journal-title":"J. Endocrinol. Investig."},{"key":"ref_5","first-page":"135","article-title":"Subacute thyroiditis after hepatitis B vaccination","volume":"45","author":"Toft","year":"1998","journal-title":"Endocr. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"dgaa276","DOI":"10.1210\/clinem\/dgaa276","article-title":"Subacute Thyroiditis After Sars-COV-2 Infection","volume":"105","author":"Brancatella","year":"2020","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"707726","DOI":"10.3389\/fendo.2021.707726","article-title":"COVID-19-Associated Subacute Thyroiditis: Evidence-Based Data from a Systematic Review","volume":"12","author":"Trimboli","year":"2021","journal-title":"Front. Endocrinol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Popescu, M., Ghemigian, A., Vasile, C.M., Costache, A., Carsote, M., and Ghenea, A.E. (2022). The New Entity of Subacute Thyroiditis amid the COVID-19 Pandemic: From Infection to Vaccine. Diagnostics, 12.","DOI":"10.3390\/diagnostics12040960"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"A956","DOI":"10.1210\/jendso\/bvab048.1954","article-title":"Subacute Thyroiditis After mRNA Vaccine for COVID-19","volume":"5","author":"Franquemont","year":"2021","journal-title":"J. Endocr. Soc."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1007\/s40618-022-01767-w","article-title":"SARS-CoV-2 vaccine-associated subacute thyroiditis","volume":"45","author":"Yorulmaz","year":"2022","journal-title":"J. Endocrinol. Investig."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"142","DOI":"10.3390\/idr14010018","article-title":"Vaccine-Induced Subacute Thyroiditis (De Quervain\u2019s) after mRNA Vaccine against SARS-CoV-2: A Case Report and Systematic Review","volume":"14","author":"Pipitone","year":"2022","journal-title":"Infect. Dis. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1007\/s40618-022-01747-0","article-title":"SARS-CoV-2 vaccine-associated subacute thyroiditis: Insights from a systematic review","volume":"45","author":"Ippolito","year":"2022","journal-title":"J. Endocrinol. Investig."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1210\/jcem-45-2-270","article-title":"Subacute (de Quervain\u2019s) thyroiditis: Association with HLA-Bw35 antigen and abnormalities of the complement system, immunoglobulins and other serum proteins","volume":"45","author":"Nyulassy","year":"1977","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/j.1399-0039.1978.tb01224.x","article-title":"HLA antigens and De Quervain\u2019s thyroiditis","volume":"11","author":"Aiginger","year":"1978","journal-title":"Tissue Antigens"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3653","DOI":"10.1210\/jcem.80.12.8530615","article-title":"Clinical characteristics of subacute thyroiditis classified according to human leukocyte antigen typing","volume":"80","author":"Ohsako","year":"1995","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1399-0039.1979.tb00823.x","article-title":"The frequency of HLA-Dw1 determinant in subacute (de Quervain\u2019s) thyroiditis","volume":"14","author":"Buc","year":"1979","journal-title":"Tissue Antigens"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1111\/j.1399-0039.1985.tb00942.x","article-title":"Genetic analysis of subacute (de Quervain\u2019s) thyroiditis","volume":"26","author":"Goto","year":"1985","journal-title":"Tissue Antigens"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Stasiak, M., Tymoniuk, B., Michalak, R., Stasiak, B., Kowalski, M.L., and Lewi\u0144ski, A. (2020). Subacute Thyroiditis is Associated with HLA-B*18:01, -DRB1*01 and -C*04:01-The Significance of the New Molecular Background. J. Clin. Med., 9.","DOI":"10.3390\/jcm9020534"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1111\/j.1399-0039.2006.00769.x","article-title":"PyPop update\u2014A software pipeline for large-scale multilocus population genomics","volume":"69","author":"Lancaster","year":"2007","journal-title":"Tissue Antigens"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/tan.12356","article-title":"The HLA-net GENE[RATE] pipeline for effective HLA data analysis and its application to 145 population samples from Europe and neighbouring areas","volume":"83","author":"Nunes","year":"2014","journal-title":"Tissue Antigens"},{"key":"ref_21","first-page":"737","article-title":"Studies on the pathogenesis of subacute thyroiditis","volume":"61","author":"Kobayashi","year":"1985","journal-title":"Nihon Naibunpi Gakkai Zasshi"},{"key":"ref_22","unstructured":"Bech, K., Nerup, J., and Molholm Hansen, J. (1976, January 23\u201325). HLA antigens in de Quervain\u2019s subacute thyroiditis. Proceedings of the 1st International Symposium on HLA and Disease, Paris, France."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1111\/j.1399-0039.2004.00258.x","article-title":"Human leukocyte antigen-A, -B and -C alleles and human leukocyte antigen haplotypes in Turkey: Relationship to other populations","volume":"64","author":"Uyar","year":"2004","journal-title":"Tissue Antigens"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1089\/thy.2022.0010","article-title":"Association of Human Leukocyte Antigen Genotypes with Severe Acute Respiratory Syndrome Coronavirus 2 Vaccine-Induced Subacute Thyroiditis","volume":"32","author":"Malkan","year":"2022","journal-title":"Thyroid"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3","DOI":"10.3389\/fendo.2019.00003","article-title":"Sonographic Pattern of Subacute Thyroiditis Is HLA-Dependent","volume":"10","author":"Stasiak","year":"2019","journal-title":"Front. Endocrinol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Stasiak, M., Tymoniuk, B., Stasiak, B., and Lewi\u0144ski, A. (2019). The Risk of Recurrence of Subacute Thyroiditis Is HLA-Dependent. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20051089"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2100","DOI":"10.1210\/jc.2002-021799","article-title":"Clinical features and outcome of subacute thyroiditis in an incidence cohort: Olmsted County, Minnesota, study","volume":"88","author":"Fatourechi","year":"2003","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"725","DOI":"10.2169\/internalmedicine.47.0740","article-title":"Clinical characteristics of 852 patients with subacute thyroiditis before treatment","volume":"47","author":"Nishihara","year":"2008","journal-title":"Intern. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"783439","DOI":"10.3389\/fendo.2021.783439","article-title":"Risk Factors for Subacute Thyroiditis Recurrence: A Systematic Review and Meta-Analysis of Cohort Studies","volume":"12","author":"Zhang","year":"2021","journal-title":"Front. Endocrinol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"16899","DOI":"10.1038\/s41598-019-53475-w","article-title":"An Evaluation of the Results of the Steroid and Non-steroidal Anti-inflammatory Drug Treatments in Subacute Thyroiditis in relation to Persistent Hypothyroidism and Recurrence","volume":"9","author":"Sencar","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Bahadir, \u00c7.T., Yilmaz, M., and Kili\u00e7kan, E. (Arch. Endocrinol. Metab., 2022). Factors affecting recurrence in subacute granulomatous thyroiditis, Arch. Endocrinol. Metab., Epub ahead of print.","DOI":"10.20945\/2359-3997000000473"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1507\/endocrj1954.35.833","article-title":"Recurrence of subacute thyroiditis over 10 years after the first attack in three cases","volume":"35","author":"Yamamoto","year":"1988","journal-title":"Endocrinol. Jpn."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Stasiak, M., Zawadzka-Starczewska, K., and Lewi\u0144ski, A. (2022). Significance of HLA Haplotypes in Two Patients with Subacute Thyroiditis Triggered by mRNA-Based COVID-19 Vaccine. Vaccines, 10.","DOI":"10.3390\/vaccines10020280"}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/10\/12\/1986\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:24:51Z","timestamp":1760145891000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/10\/12\/1986"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,23]]},"references-count":33,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["vaccines10121986"],"URL":"https:\/\/doi.org\/10.3390\/vaccines10121986","relation":{},"ISSN":["2076-393X"],"issn-type":[{"value":"2076-393X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,23]]}}}