{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:54:11Z","timestamp":1760147651583,"version":"build-2065373602"},"reference-count":89,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T00:00:00Z","timestamp":1676937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health","award":["U19 AI144184","DE-AC52-07NA27344"],"award-info":[{"award-number":["U19 AI144184","DE-AC52-07NA27344"]}]},{"name":"U.S. Department of Energy by Lawrence Livermore National Laboratory","award":["U19 AI144184","DE-AC52-07NA27344"],"award-info":[{"award-number":["U19 AI144184","DE-AC52-07NA27344"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Chlamydia trachomatis is the most common bacterial sexually transmitted pathogen. The number of chlamydial infections continuous to increase and there is an urgent need for a safe and efficacious vaccine. To assess the ability of the Chlamydia muridarum polymorphic membrane protein G (PmpG) and the plasmid glycoprotein 3 (Pgp3) as single antigens, and in combination with the major outer-membrane protein (MOMP) to induce protection, BALB\/c mice were immunized utilizing CpG-1826 and Montanide ISA 720 VG as adjuvants. Following vaccination with MOMP, significant humoral and cell-mediated immune responses were observed, while immunization with PmpG, or Pgp3, elicited weaker immune responses. Weaker immune responses were induced with MOMP+Pgp3 compared with MOMP alone. Following the intranasal challenge with C. muridarum, mice vaccinated with MOMP showed robust protection against body-weight loss, inflammatory responses in the lungs and number of Chlamydia recovered from the lungs. PmpG and Pgp3 elicited weaker protective responses. Mice immunized with MOMP+PmpG, were no better protected than animals vaccinated with MOMP only, while Pgp3 antagonized the protection elicited by MOMP. In conclusion, PmpG and Pgp3 elicited limited protective immune responses in mice against a respiratory challenge with C. muridarum and failed to enhance the protection induced by MOMP alone. The virulence of Pgp3 may result from its antagonistic effect on the immune protection induced by MOMP.<\/jats:p>","DOI":"10.3390\/vaccines11030504","type":"journal-article","created":{"date-parts":[[2023,2,22]],"date-time":"2023-02-22T03:32:17Z","timestamp":1677036737000},"page":"504","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Polymorphic Membrane Protein G Has a Neutral Effect and the Plasmid Glycoprotein 3 an Antagonistic Effect on the Ability of the Major Outer Membrane Protein to Elicit Protective Immune Responses against a Chlamydia muridarum Respiratory Challenge"],"prefix":"10.3390","volume":"11","author":[{"given":"Anatoli","family":"Slepenkin","sequence":"first","affiliation":[{"name":"Department of Pathology, Laboratory Medicine, Medical Sciences I, Room D440, University of California, Irvine, CA 92697, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sukumar","family":"Pal","sequence":"additional","affiliation":[{"name":"Department of Pathology, Laboratory Medicine, Medical Sciences I, Room D440, University of California, Irvine, CA 92697, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Hoang-Phou","sequence":"additional","affiliation":[{"name":"Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abisola","family":"Abisoye-Ogunniyan","sequence":"additional","affiliation":[{"name":"Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy","family":"Rasley","sequence":"additional","affiliation":[{"name":"Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrik","family":"D\u2019haeseleer","sequence":"additional","affiliation":[{"name":"Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1389-4018","authenticated-orcid":false,"given":"Matthew A.","family":"Coleman","sequence":"additional","affiliation":[{"name":"Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA"},{"name":"Department of Radiation Oncology, School of Medicine, University of California, Davis, CA 95616, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis M.","family":"de la Maza","sequence":"additional","affiliation":[{"name":"Department of Pathology, Laboratory Medicine, Medical Sciences I, Room D440, University of California, Irvine, CA 92697, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,21]]},"reference":[{"key":"ref_1","unstructured":"CDC (2021). 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