{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T04:22:44Z","timestamp":1774585364932,"version":"3.50.1"},"reference-count":57,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T00:00:00Z","timestamp":1680220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Biotechnology, Ministry of Science and Technology, Government of India","award":["BT\/IN\/EU-INF\/17\/GK\/19-20"],"award-info":[{"award-number":["BT\/IN\/EU-INF\/17\/GK\/19-20"]}]},{"name":"Department of Biotechnology, Ministry of Science and Technology, Government of India","award":["H2020-SC1-2019-874653-INDIGO"],"award-info":[{"award-number":["H2020-SC1-2019-874653-INDIGO"]}]},{"name":"Department of Biotechnology, Ministry of Science and Technology, Government of India","award":["BT\/IN\/EU-INF\/15\/RV\/19-20"],"award-info":[{"award-number":["BT\/IN\/EU-INF\/15\/RV\/19-20"]}]},{"name":"Department of Biotechnology, Ministry of Science and Technology, Government of India","award":["H2020-SC1-2019-874650-ENDFLU"],"award-info":[{"award-number":["H2020-SC1-2019-874650-ENDFLU"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Immunogens mimicking the native-like structure of surface-exposed viral antigens are considered promising vaccine candidates. Influenza viruses are important zoonotic respiratory viruses with high pandemic potential. Recombinant soluble hemagglutinin (HA) glycoprotein-based protein subunit vaccines against Influenza have been shown to induce protective efficacy when administered intramuscularly. Here, we have expressed a recombinant soluble trimeric HA protein in Expi 293F cells and purified the protein derived from the Inf A\/Guangdong-Maonan\/ SWL1536\/2019 virus which was found to be highly virulent in the mouse. The trimeric HA protein was found to be in the oligomeric state, highly stable, and the efficacy study in the BALB\/c mouse challenge model through intradermal immunization with the prime-boost regimen conferred complete protection against a high lethal dose of homologous and mouse-adapted InfA\/PR8 virus challenge. Furthermore, the immunogen induced high hemagglutinin inhibition (HI) titers and showed cross-protection against other Inf A and Inf B subtypes. The results are promising and warrant trimeric HA as a suitable vaccine candidate.<\/jats:p>","DOI":"10.3390\/vaccines11040780","type":"journal-article","created":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T03:08:31Z","timestamp":1680491311000},"page":"780","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Intradermal Immunization of Soluble Influenza HA Derived from a Lethal Virus Induces High Magnitude and Breadth of Antibody Responses and Provides Complete Protection In Vivo"],"prefix":"10.3390","volume":"11","author":[{"given":"Sneha","family":"Raj","sequence":"first","affiliation":[{"name":"Translational Health Science & Technology Institute, NCR Biotech Science Cluster, Faridabad 121001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8530-4860","authenticated-orcid":false,"given":"Preeti","family":"Vishwakarma","sequence":"additional","affiliation":[{"name":"Translational Health Science & Technology Institute, NCR Biotech Science Cluster, Faridabad 121001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shikha","family":"Saxena","sequence":"additional","affiliation":[{"name":"Translational Health Science & Technology Institute, NCR Biotech Science Cluster, Faridabad 121001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Varun","family":"Kumar","sequence":"additional","affiliation":[{"name":"Translational Health Science & Technology Institute, NCR Biotech Science Cluster, Faridabad 121001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ritika","family":"Khatri","sequence":"additional","affiliation":[{"name":"Translational Health Science & Technology Institute, NCR Biotech Science Cluster, Faridabad 121001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit","family":"Kumar","sequence":"additional","affiliation":[{"name":"Translational Health Science & Technology Institute, NCR Biotech Science Cluster, Faridabad 121001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mrityunjay","family":"Singh","sequence":"additional","affiliation":[{"name":"Translational Health Science & Technology Institute, NCR Biotech Science Cluster, Faridabad 121001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surbhi","family":"Mishra","sequence":"additional","affiliation":[{"name":"Translational Health Science & Technology Institute, NCR Biotech Science Cluster, Faridabad 121001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shailendra","family":"Asthana","sequence":"additional","affiliation":[{"name":"Translational Health Science & Technology Institute, NCR Biotech Science Cluster, Faridabad 121001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shubbir","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Centralized Core Research Facility (CCRF), All India Institute of Medical Sciences, New Delhi 110029, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sweety","family":"Samal","sequence":"additional","affiliation":[{"name":"Translational Health Science & Technology Institute, NCR Biotech Science Cluster, Faridabad 121001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1186\/s13054-019-2539-x","article-title":"Influenza virus-related critical illness: Pathophysiology and epidemiology","volume":"23","author":"Kalil","year":"2019","journal-title":"Crit. 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