{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T16:26:34Z","timestamp":1788279994509,"version":"build-2803163510"},"reference-count":40,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,4]],"date-time":"2023-04-04T00:00:00Z","timestamp":1680566400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>The objectives of the present study were (a) to evaluate the importance of climate-related variables in the vaccination patterns applied in sheep and goat farms in Greece and (b) to assess potential interactions between these factors and previously established important health management- and human resources-related factors applied in the farms. Vaccination patterns against chlamydial abortion, clostridial infections, contagious agalactia, contagious ecthyma, foot-rot, paratuberculosis, pneumonia or staphylococcal mastitis were assessed. Climatic variables (2010\u20132019 and 2018\u20132019) were obtained for 444 locations with small ruminant farms throughout Greece. Patterns of vaccine administration in the farms were obtained through interviews with farmers. The following nine outcomes were considered: \u2018vaccination against chlamydial abortion\u2019, \u2018vaccination against clostridial infections\u2019, \u2018vaccination against contagious agalactia\u2019, \u2018vaccination against contagious ecthyma\u2019, \u2018vaccination against foot-rot\u2019, \u2018vaccination against paratuberculosis\u2019, \u2018vaccination against bacterial pneumonia\u2019, \u2018vaccination against staphylococcal mastitis\u2019 and \u2018total number of optional vaccines administered\u2019. Univariable and multivariable analyses were first performed to establish associations of each of the above outcomes with climatic variables. Then, the same approach was employed to assess the importance of climatic variables against health management- and human resources-related factors in the administration of vaccines in the farms of the study. Climatic variables had a higher association with vaccinations against infections in sheep flocks (26 associations) than in goat herds (9 associations) (p = 0.002) and in farms with semi-extensive or extensive management (32 associations) than in farms with intensive or semi-intensive management (8 associations) (p &lt; 0.0001). In 26 cases (38.8% of all analyses evaluated), the climatic variables overshadowed the management- and human resources-related variables assessed as significant predictors for vaccination. In most cases, these referred to sheep flocks (nine cases) and farms with semi-extensive or extensive management (eight cases). For all eight infections, there were changes in the climatic variables found to be significant predictors from the 10-year dataset to the 2-year dataset. The results indicated that, in some cases, climate factors overshadowed factors traditionally considered for the formulation of vaccination programs. This points out the significance of taking into account climate conditions in the health management of small ruminant farms. Future studies can be focused on formulating vaccination programs in accordance with climate factors and also on setting the optimum season(s) for vaccination of animals based on the circulation of the pathogens, the risks for the development of diseases and the stage within the annual production cycle of the animals.<\/jats:p>","DOI":"10.3390\/vaccines11040797","type":"journal-article","created":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T02:59:43Z","timestamp":1680663583000},"page":"797","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Relationship between Vaccine Application and Climate Factors in Sheep and Goat Farms in Greece"],"prefix":"10.3390","volume":"11","author":[{"given":"Eleni I.","family":"Katsarou","sequence":"first","affiliation":[{"name":"Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0214-6332","authenticated-orcid":false,"given":"George C.","family":"Fthenakis","sequence":"additional","affiliation":[{"name":"Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Quintana, A.R., Sese\u00f1a, S., Garz\u00f3n, A., and Arias, R. (2020). Factors affecting levels of airborne bacteria in dairy farms: A review. Animals, 10.","DOI":"10.3390\/ani10030526"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100372","DOI":"10.1016\/j.onehlt.2022.100372","article-title":"The association between natural drinking water sources and the emergence of zoonotic leptospirosis among grazing beef cattle herds during a human outbreak","volume":"14","author":"Zamir","year":"2022","journal-title":"One Health"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"170808","DOI":"10.1098\/rsos.170808","article-title":"Seasonality and pathogen transmission in pastoral cattle contact networks","volume":"4","author":"VanderWaal","year":"2017","journal-title":"R. Soc. Open. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1139\/y98-097","article-title":"Cold exposure and immune function","volume":"76","author":"Shephard","year":"1998","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/s41541-021-00354-z","article-title":"Vaccination as a preventative measure contributing to immune fitness","volume":"6","author":"Doherty","year":"2021","journal-title":"NPJ Vaccines"},{"key":"ref_6","unstructured":"Tizard, I.R. (2021). Vaccines for Veterinarians, Elsevier."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.prevetmed.2018.04.004","article-title":"Retrospective analysis of Bluetongue farm risk profile definition, based on biology, farm management practices and climatic data","volume":"155","author":"Cappai","year":"2018","journal-title":"Prev. Vet. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4249","DOI":"10.1038\/s41598-019-40450-8","article-title":"Livestock host composition rather than land use or climate explains spatial patterns in bluetongue disease in South India","volume":"9","author":"Chanda","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1186\/s13071-021-04945-6","article-title":"Modelling bluetongue risk in Kazakhstan","volume":"14","author":"Abdrakhmanov","year":"2021","journal-title":"Parasites Vectors"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"106804","DOI":"10.1016\/j.smallrumres.2022.106804","article-title":"Factors driving adoption of climatic risk mitigating technologies with special reference to goat farming in India: Evidence from meta-analysis","volume":"216","author":"Thirunavukkarasu","year":"2022","journal-title":"Small Rumin. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1111\/irv.12393","article-title":"Influenza activity in Kenya, 2007-2013: Timing, association with climatic factors, and implications for vaccination campaigns","volume":"10","author":"Emukule","year":"2016","journal-title":"Influenza Other Respir. Viruses"},{"key":"ref_12","unstructured":"Ministry of Agricultural Development & Food (2018). Greek Agriculture\u2014Animal Production."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Lianou, D.T., Michael, C.K., Petinaki, E., Mavrogianni, V.S., and Fthenakis, G.C. (2022). Administration of vaccines in dairy sheep and goat farms: Patterns of vaccination, associations with health and production parameters, predictors. Vaccines, 10.","DOI":"10.3390\/vaccines10091372"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Lianou, D.T., Chatziprodromidou, I.P., Vasileiou, N.G.C., Michael, C.K., Mavrogianni, V.S., Politis, A.P., Kordalis, N.G., Billinis, C., Giannakopoulos, A., and Papadopoulos, E. (2020). A detailed questionnaire for the evaluation of health management in dairy sheep and goats. Animals, 10.","DOI":"10.3390\/ani10091489"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Katsarou, E.I., Lianou, D.T., Papadopoulos, E., and Fthenakis, G.C. (2022). Long-term climatic changes in small ruminant farms in Greece and potential associations with animal health. Sustainability, 14.","DOI":"10.3390\/su14031673"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (2014). Scientific opinion on the welfare risks related to the farming of sheep for wool, meat and milk production. EFSA J., 12, 3933\u20134060.","DOI":"10.2903\/j.efsa.2014.3933"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Impoinvil, D.E., Ooi, M.H., Diggle, P.J., Caminade, C., Cardosa, M.J., Morse, A.P., Baylis, M., and Solomon, T. (2013). The effect of vaccination coverage and climate on japanese encephalitis in Sarawak, Malaysia. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002334"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.smallrumres.2008.08.004","article-title":"Influence of climatic factors on the development of pneumonia in lambs","volume":"80","author":"Lacasta","year":"2008","journal-title":"Small Rumin. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.cimid.2018.11.011","article-title":"Study of potential environmental factors predisposing ewes to subclinical mastitis in Greece","volume":"62","author":"Vasileiou","year":"2019","journal-title":"Comp. Immunol. Microbiol. Inf. Dis."},{"key":"ref_20","unstructured":"MetOffice United Kingdom (2022, December 17). Weather Lore, Available online: https:\/\/www.metoffice.gov.uk\/research\/library-and-archive\/archive-hidden-treasures\/weather-lore#:~:text=It%20consists%20of%20a%20wide,on%20astronomy%2C%20astrology%20and%20clouds."},{"key":"ref_21","unstructured":"Anagnostopoulos, L. (2014). Folk Culture in Vytina Peloponnese. Traditions and Customs, Private publication."},{"key":"ref_22","unstructured":"Carouzou, V. (2022, December 17). Cliche Imerominia and Weather Lore. Available online: https:\/\/www.slideshare.net\/vivicarouzou\/erasmus-cliche-imerominia-and-weather-lore."},{"key":"ref_23","unstructured":"Alc\u00f3n, M.B. (2005). Lo de Mora, Trafford Publishing."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Lianou, D.T., Michael, C.K., Vasileiou, N.G.C., Liagka, D.V., Mavrogianni, V.S., Caroprese, M., and Fthenakis, G.C. (2021). Association of breed of sheep or goats with somatic cell counts and total bacterial counts of bulk-tank milk. Appl. Sci., 11.","DOI":"10.3390\/app11167356"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"24","DOI":"10.3389\/fgene.2015.00024","article-title":"Recent advances in understanding the genetic resources of sheep breeds locally-adapted to the UK uplands: Opportunities they offer for sustainable productivity","volume":"6","author":"Bowles","year":"2015","journal-title":"Front. Genet."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"207","DOI":"10.5194\/aab-65-207-2022","article-title":"Status quo of genetic improvement in local goats: A review","volume":"65","year":"2022","journal-title":"Arch Anim. Breed."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1017\/S0022029918000407","article-title":"Association of subclinical mastitis prevalence with sheep breeds in Greece","volume":"85","author":"Vasileiou","year":"2018","journal-title":"J. Dairy Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2063","DOI":"10.12681\/jhvms.22967","article-title":"Genetic improvement of indigenous Greek sheep and goat breeds","volume":"71","author":"Argyriadou","year":"2020","journal-title":"J. Hell. Vet. Med. Soc."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Berihulay, H., Abied, A., He, X., Jiang, L., and Ma, Y. (2019). Adaptation mechanisms of small ruminants to environmental heat stress. Animals, 9.","DOI":"10.3390\/ani9030075"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Narayan, E. (2019). Comparative Endocrinology of Animals, Intechopen.","DOI":"10.5772\/intechopen.73427"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"692121","DOI":"10.3389\/fgene.2021.692121","article-title":"Breeding strategies for weather resilience in small ruminants in Atlantic and Mediterranean climates","volume":"12","author":"Kapsona","year":"2021","journal-title":"Front. Genet."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"21363","DOI":"10.1038\/s41598-021-00682-z","article-title":"Local adaptations of Mediterranean sheep and goats through an integrative approach","volume":"11","author":"Serranito","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"297","DOI":"10.3389\/fgene.2019.00297","article-title":"Livestock genomics for developing countries\u2013African examples in practice","volume":"10","author":"Marshall","year":"2019","journal-title":"Front. Genet."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.smallrumres.2016.10.004","article-title":"Patterns of heat tolerance in different sheep breeds in Brazil","volume":"144","author":"McManus","year":"2016","journal-title":"Small Rumin. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1017\/S0021859600027908","article-title":"Heat tolerance of different genetic groups of sheep exposed to elevated temperature conditions","volume":"94","author":"Singh","year":"1980","journal-title":"J. Agric. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Lianou, D.T., and Fthenakis, G.C. (2021). Dairy sheep and goat farmers: Socio-demographic characteristics and their associations with health management and performance on farms. Land, 10.","DOI":"10.3390\/land10121358"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.vetmic.2015.08.004","article-title":"Bluetongue in small ruminants: An opinionated review, with a brief appraisal of the 2014 outbreak of the disease in Greece and the south-east Europe","volume":"181","author":"Kyriakis","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.smallrumres.2016.02.010","article-title":"Experiences from the 2014 outbreak of bluetongue in Greece","volume":"142","author":"Vasileiou","year":"2016","journal-title":"Small Rumin. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1093\/af\/vfab065","article-title":"Opportunities for precision livestock management in the face of climate change: A focus on extensive systems","volume":"11","author":"Williams","year":"2021","journal-title":"Anim. Front."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"6","DOI":"10.2527\/af.2017.0102","article-title":"General introduction to precision livestock farming","volume":"7","author":"Berckmans","year":"2017","journal-title":"Anim. Front."}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/11\/4\/797\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:09:53Z","timestamp":1760123393000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/11\/4\/797"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,4]]},"references-count":40,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["vaccines11040797"],"URL":"https:\/\/doi.org\/10.3390\/vaccines11040797","relation":{},"ISSN":["2076-393X"],"issn-type":[{"value":"2076-393X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,4]]}}}