{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:41:09Z","timestamp":1790664069323,"version":"4.1.0"},"reference-count":53,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T00:00:00Z","timestamp":1684108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"French National Association for Research and Technology (ANRT)"},{"name":"Boehringer Ingelheim through an industrial (CIFRE) PhD grant"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Bordetella bronchiseptica (Bb) is a Gram-negative bacterium responsible for canine infectious respiratory disease complex (CIRDC). Several vaccines targeting this pathogen are currently licensed for use in dogs, but their mechanism of action and the correlates of protection are not fully understood. To investigate this, we used a rat model to examine the immune responses induced and the protection conferred by a canine mucosal vaccine after challenge. Wistar rats were vaccinated orally or intranasally on D0 and D21 with a live attenuated Bb vaccine strain. At D35, the rats of all groups were inoculated with 103 CFU of a pathogenic strain of B. bronchiseptica. Animals vaccinated via either the intranasal or the oral route had Bb-specific IgG and IgM in their serum and Bb-specific IgA in nasal lavages. Bacterial load in the trachea, lung, and nasal lavages was lower in vaccinated animals than in non-vaccinated control animals. Interestingly, coughing improved in the group vaccinated intranasally, but not in the orally vaccinated or control group. These results suggest that mucosal vaccination can induce mucosal immune responses and provide protection against a Bb challenge. This study also highlights the advantages of a rat model as a tool for studying candidate vaccines and routes of administration for dogs.<\/jats:p>","DOI":"10.3390\/vaccines11050982","type":"journal-article","created":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T02:37:51Z","timestamp":1684204671000},"page":"982","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Mucosal Vaccination with Live Attenuated Bordetella bronchiseptica Protects against Challenge in Wistar Rats"],"prefix":"10.3390","volume":"11","author":[{"given":"Beatriz Miguelena","family":"Chamorro","sequence":"first","affiliation":[{"name":"CIRI\u2014Centre International de Recherche en Infectiologie, Team GIMAP (Saint-Etienne), Universit\u00e9 Claude Bernard Lyon 1, Inserm, U1111, CNRS, UMR5308, ENS Lyon, UJM, F69007 Lyon, France"},{"name":"Boehringer Ingelheim, Global Innovation, F69800 Saint Priest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karelle De","family":"Luca","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim, Global Innovation, F69800 Saint Priest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gokul","family":"Swaminathan","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim, Global Innovation, F69800 Saint Priest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Rochereau","sequence":"additional","affiliation":[{"name":"CIRI\u2014Centre International de Recherche en Infectiologie, Team GIMAP (Saint-Etienne), Universit\u00e9 Claude Bernard Lyon 1, Inserm, U1111, CNRS, UMR5308, ENS Lyon, UJM, F69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jade","family":"Majorel","sequence":"additional","affiliation":[{"name":"CIRI\u2014Centre International de Recherche en Infectiologie, Team GIMAP (Saint-Etienne), Universit\u00e9 Claude Bernard Lyon 1, Inserm, U1111, CNRS, UMR5308, ENS Lyon, UJM, F69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7614-9897","authenticated-orcid":false,"given":"Herv\u00e9","family":"Poulet","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim, Global Innovation, F69800 Saint Priest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blandine","family":"Chanut","sequence":"additional","affiliation":[{"name":"CIRI\u2014Centre International de Recherche en Infectiologie, Team GIMAP (Saint-Etienne), Universit\u00e9 Claude Bernard Lyon 1, Inserm, U1111, CNRS, UMR5308, ENS Lyon, UJM, F69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauriane","family":"Piney","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim, Global Innovation, F69800 Saint Priest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egbert","family":"Mundt","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim, Global Innovation, F69800 Saint Priest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8830-4273","authenticated-orcid":false,"given":"St\u00e9phane","family":"Paul","sequence":"additional","affiliation":[{"name":"CIRI\u2014Centre International de Recherche en Infectiologie, Team GIMAP (Saint-Etienne), Universit\u00e9 Claude Bernard Lyon 1, Inserm, U1111, CNRS, UMR5308, ENS Lyon, UJM, F69007 Lyon, France"},{"name":"3CIC Inserm 1408 Vaccinology, F42023 Saint-Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1128\/CMR.18.2.326-382.2005","article-title":"Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due to Bordetella pertussis and Other Bordetella Subspecies","volume":"18","author":"Mattoo","year":"2005","journal-title":"Clin. 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