{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T07:01:19Z","timestamp":1788850879866,"version":"build-2803163510"},"reference-count":50,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,5,21]],"date-time":"2023-05-21T00:00:00Z","timestamp":1684627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Understanding the immunological mechanisms of protection and the viral proteins involved in the induction of a protective immune response to the African swine fever virus (ASFV) is still limited. In the last years, the CD2v protein (gp110-140) of the ASFV has been proven to be a serotype-specific protein. Current work is devoted to the investigation of the possibility of creating protection against virulent ASFV strain Mozambique-78 (seroimmunotype III) in pigs previously vaccinated with vaccine strain FK-32\/135 (seroimmunotype IV) and then immunized with the pUBB76A_CD2v plasmid, containing a chimeric nucleotide sequence from the CD2v protein gene (EP402R, nucleotides from 49 to 651) from the MK-200 strain (seroimmunotype III). Vaccination with the ASFV vaccine strain FK-32\/135 protects pigs from the disease caused by the strain with homologous seroimmunotype\u2014France-32 (seroimmunotype IV). Our attempt to create balanced protection against virulent strain Mozambique-78 (seroimmunotype III) by induction of both humoral factors of immunity (by vaccination with strain FK-32\/135 of seroimmunotype IV) and serotype-specific cellular immunity (by immunization with the plasmid pUBB76A_CD2v of seroimmunotype III) was unsuccessful.<\/jats:p>","DOI":"10.3390\/vaccines11051007","type":"journal-article","created":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T01:31:46Z","timestamp":1684719106000},"page":"1007","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Subsequent Immunization of Pigs with African Swine Fever Virus (ASFV) Seroimmunotype IV Vaccine Strain FK-32\/135 and by Recombinant Plasmid DNA Containing the CD2v Derived from MK-200 ASFV Seroimmunotype III Strain Does Not Protect from Challenge with ASFV Seroimmunotype III"],"prefix":"10.3390","volume":"11","author":[{"given":"Alexey","family":"Sereda","sequence":"first","affiliation":[{"name":"Federal Research Center for Virology and Microbiology (FRCVM), 601125 Volginsky, Petushki Area, Vladimir Region, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Kazakova","sequence":"additional","affiliation":[{"name":"Federal Research Center for Virology and Microbiology (FRCVM), 601125 Volginsky, Petushki Area, Vladimir Region, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanzhi","family":"Namsrayn","sequence":"additional","affiliation":[{"name":"Federal Research Center for Virology and Microbiology (FRCVM), 601125 Volginsky, Petushki Area, Vladimir Region, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail","family":"Vlasov","sequence":"additional","affiliation":[{"name":"Federal Research Center for Virology and Microbiology (FRCVM), 601125 Volginsky, Petushki Area, Vladimir Region, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5947-9402","authenticated-orcid":false,"given":"Irina","family":"Sindryakova","sequence":"additional","affiliation":[{"name":"Federal Research Center for Virology and Microbiology (FRCVM), 601125 Volginsky, Petushki Area, Vladimir Region, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4935-0891","authenticated-orcid":false,"given":"Denis","family":"Kolbasov","sequence":"additional","affiliation":[{"name":"Federal Research Center for Virology and Microbiology (FRCVM), 601125 Volginsky, Petushki Area, Vladimir Region, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,21]]},"reference":[{"key":"ref_1","first-page":"104","article-title":"Haemadsorbtion and cytopathic effect produced by ASFV in swine bone marrow and buffy coat cultures","volume":"21","author":"Malmquist","year":"1960","journal-title":"Am. 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