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China","award":["KJQN202203502"],"award-info":[{"award-number":["KJQN202203502"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Tilapia, as one of the fish widely cultured around the world, is suffering severe impact from the streptococcus disease with the deterioration of the breeding environment and the increasing of breeding density, which brings serious economic loss to tilapia farming. In this study, the surface immunogenic protein (Sip) of Streptococcus agalactiae (S. agalactiae) was selected as the potential candidate antigen and connected with bacterial nano cellulose (BNC) to construct the nanocarrier subunit vaccine (BNC-rSip), and the immersion immune effects against S. agalactiae and Streptococcus iniae (S. iniae) in Nile tilapia were evaluated on the basis of the serum antibody level, non-specific enzyme activity, the immune-related gene expression and relative percent survival (RPS). The results indicated that Sip possessed the expected immunogenicity according to the immunoinformatic analysis. Compared with the rSip group, BNC-rSip significantly induced serum antibody production and improved the innate immunity level of tilapia. After challenge, the RPS of BNC-rSip groups were 78.95% (S. agalactiae) and 67.86% (S. iniae), which were both higher than those of rSip groups,31.58% (S. agalactiae) and 35.71% (S. iniae), respectively. Our study indicated that BNC-rSip can induce protective immunity for tilapia through immersion immunization and may be an ideal candidate vaccine for controlling tilapia streptococcal disease.<\/jats:p>","DOI":"10.3390\/vaccines11071262","type":"journal-article","created":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T05:42:01Z","timestamp":1689831721000},"page":"1262","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Exploring the Immunoprotective Potential of a Nanocarrier Immersion Vaccine Encoding Sip against Streptococcus Infection in Tilapia (Oreochromis niloticus)"],"prefix":"10.3390","volume":"11","author":[{"given":"Ye","family":"Cao","sequence":"first","affiliation":[{"name":"Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"},{"name":"Northwest A&F University Shenzhen Research Institute, Shenzhen 518000, China"},{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Liu","sequence":"additional","affiliation":[{"name":"Northwest A&F University Shenzhen Research Institute, Shenzhen 518000, China"},{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaoyang","family":"Liu","sequence":"additional","affiliation":[{"name":"Northwest A&F University Shenzhen Research Institute, Shenzhen 518000, China"},{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Du","sequence":"additional","affiliation":[{"name":"Northwest A&F University Shenzhen Research Institute, Shenzhen 518000, China"},{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5042-4093","authenticated-orcid":false,"given":"Tianqiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Northwest A&F University Shenzhen Research Institute, Shenzhen 518000, China"},{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaoxue","family":"Wang","sequence":"additional","affiliation":[{"name":"Northwest A&F University Shenzhen Research Institute, Shenzhen 518000, China"},{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing 404155, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erlong","family":"Wang","sequence":"additional","affiliation":[{"name":"Northwest A&F University Shenzhen Research Institute, Shenzhen 518000, China"},{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.aquaculture.2016.10.022","article-title":"Additive genetic variation in resistance of Nile tilapia (Oreochromis niloticus) to Streptococcus iniae and S-agalactiae capsular type Ib: Is genetic resistance correlated?","volume":"468","author":"Shoemaker","year":"2017","journal-title":"Aquaculture"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.fsi.2017.01.023","article-title":"Transcriptomic profiling analysis of tilapia (Oreochromis niloticus) following Streptococcus agalactiae challenge","volume":"62","author":"Zhu","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1007\/s12562-011-0365-4","article-title":"Identification and molecular typing of Streptococcus agalactiae isolated from pond-cultured tilapia in China","volume":"77","author":"Ye","year":"2011","journal-title":"Fish. 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