{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T20:19:55Z","timestamp":1789676395399,"version":"4.0.1"},"reference-count":85,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T00:00:00Z","timestamp":1696464000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CAPES (Coordination for the Improvement of Higher Education Personnel, Brazil)"},{"name":"CNPq (National Council for Scientific and Technological Development)"},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa de Minas Gerais (FAPEMIG)"},{"name":"Departamento de Ci\u00eancia e Tecnologia do Minist\u00e9rio da Sa\u00fade do Brasil"},{"name":"QUATREE (GranvitaPet)"},{"name":"Universidade Federal de Minas Gerais (UFMG)"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Doen\u00e7as Tropicais (INCT-DT)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Dogs with visceral leishmaniasis play a key role in the transmission cycle of Leishmania infantum to humans in the urban environment. There is a consensus regarding the importance of developing a vaccine to control this disease. Despite many efforts to develop a protective vaccine against CVL, the ones currently available, Leish-tec\u00ae and LetiFend\u00ae, have limited effectiveness. This is due, in part, to the complexity of the immune response of the naturally infected dogs against the parasite and the complexity of the parasite transmission cycle. Thus, strategies, such as the development of a transmission-blocking vaccines (TBVs) already being applied to other vector-borne diseases like malaria and dengue, would be an attractive alternative to control leishmaniasis. TBVs induce the production of antibodies in the vertebrate host, which can inhibit parasite development in the vector and\/or interfere with aspects of vector biology, leading to an interruption of parasite transmission. To date, there are few TBV studies for CVL and other leishmaniasis forms. However, the few studies that exist show promising results, thus justifying the further development of this approach.<\/jats:p>","DOI":"10.3390\/vaccines11101565","type":"journal-article","created":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T09:14:22Z","timestamp":1696497262000},"page":"1565","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Transmission-Blocking Vaccines for Canine Visceral Leishmaniasis: New Progress and Yet New Challenges"],"prefix":"10.3390","volume":"11","author":[{"given":"Jaqueline Costa","family":"Leite","sequence":"first","affiliation":[{"name":"Laboratory of Biology of Cell Interactions, Department of Morphology, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3374-5418","authenticated-orcid":false,"given":"Ana Alice 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Trop. Med. Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1093\/intimm\/dxx075","article-title":"How to master the host immune system? Leishmania parasites have the solutions!","volume":"30","author":"Rossi","year":"2017","journal-title":"Int. Immunol."},{"key":"ref_3","first-page":"957","article-title":"Sur un protozaire nouveau (Piroplasma donovani Lav. & Mesn.). Parasite d\u2019 une fi\u00e8vrew de I\u2019Inde","volume":"137","author":"Laveran","year":"1903","journal-title":"Comp. R. H\u00e9bd. S\u00e9anc. Acad. Sci."},{"key":"ref_4","first-page":"121","article-title":"Culture des corps de Leishman isoles de la rate dans trois c\u00e3s d\u2019anemicsptenique infantile","volume":"1","author":"Nicolle","year":"1908","journal-title":"Bull. Soc. Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"272","DOI":"10.3389\/fimmu.2014.00272","article-title":"Immunotherapy and immunochemotherapy in visceral leishmaniasis: Promising treatments for this neglected disease","volume":"5","author":"Roatt","year":"2014","journal-title":"Front. Immunol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1590\/0037-8682-0120-2016","article-title":"Recent updates and perspectives on approaches for the development of vaccines against visceral leishmaniasis","volume":"49","author":"Duarte","year":"2016","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Scarpini, S., Dondi, A., Totaro, C., Biagi, C., Melchionda, F., Zama, D., Pierantoni, L., Gennari, M., Campagna, C., and Prete, A. (2022). Visceral Leishmaniasis: Epidemiology, Diagnosis, and Treatment Regimens in Different Geographical Areas with a Focus on Pediatrics. Microorganisms, 10.","DOI":"10.3390\/microorganisms10101887"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.vetpar.2012.06.025","article-title":"Temporal distribution of positive results of tests for detecting Leishmania infection in stray dogs of an endemic area of visceral leishmaniasis in the Brazilian tropics: A 13 years survey and association with human disease","volume":"21","author":"Fraga","year":"2012","journal-title":"Vet. Parasitol."},{"key":"ref_9","first-page":"329","article-title":"Estudos sobre o parasite. Leishmaniose visceral americana, nova entidade m\u00f3rbida do homem na Am\u00e9rica do Sul","volume":"32","author":"Cunha","year":"1937","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/S0169-4758(00)01637-9","article-title":"The strange case of Leishmania chagasi","volume":"16","author":"Stothard","year":"2000","journal-title":"Parasitol. Today"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.actatropica.2016.09.014","article-title":"Dispersal of Lutzomyia longipalpis and expansion of canine and human visceral leishmaniasis in S\u00e3o Paulo State, Brazil","volume":"164","author":"Oliveira","year":"2016","journal-title":"Acta Trop."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1590\/S0074-02761912000100006","article-title":"Contribui\u00e7\u00e3o para o conhecimento das esp\u00e9cies do genero Phlebotomus existentes no Brazil","volume":"4","author":"Lutz","year":"1912","journal-title":"Mem\u00f3rias Do Inst. Oswaldo Cruz"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1590\/S0001-37652003000300005","article-title":"Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae): A review","volume":"75","author":"Soares","year":"2003","journal-title":"Acad. Bras. Ci\u00eanc"},{"key":"ref_14","unstructured":"WHO (2023, May 09). Leishmaniasis: Key Facts. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/leishmaniasis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.pt.2018.11.005","article-title":"Culling Dogs for Zoonotic Visceral Leishmaniasis Control: The Wind of Change","volume":"35","author":"Bowman","year":"2019","journal-title":"Trends Parasitol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"53","DOI":"10.4269\/ajtmh.1998.59.53","article-title":"Studies on Control of Visceral Leishmaniasis: Impact of Dog Control on Canine and Human Visceral Leishmaniasis in Jacobina, Bahia, Brazil","volume":"59","author":"Ashford","year":"1998","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1590\/S0001-37652004000300012","article-title":"Improving methods for epidemiological control of canine visceral leishmaniasis based on a mathematical model. Impact on the incidence of the canine and human disease","volume":"76","author":"Palatnik","year":"2004","journal-title":"Acad. Bras. Cienc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.vetpar.2010.01.044","article-title":"Relationship between dog culling and incidence of human visceral leishmaniasis in an endemic area","volume":"170","author":"Nunes","year":"2010","journal-title":"Vet. Parasitol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Romero, G.A., and Boelaert, M. (2010). Control of visceral leishmaniasis in latin america-a systematic review. PLoS Negl. Trop. Dis., 19.","DOI":"10.1371\/journal.pntd.0000584"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1590\/S0037-86822011005000014","article-title":"How effective is dog culling in controlling zoonotic visceral leishmaniasis? A critical evaluation of the science, politics and ethics behind this public health policy","volume":"44","author":"Costa","year":"2011","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.rvsc.2019.01.022","article-title":"Current status and management of canine leishmaniasis in Latin America","volume":"123","author":"Marcondes","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1590\/1678-5150-pvb-6165","article-title":"Evaluation of the Euthanasia of Seropositive Dogs for Canine Visceral Leishmaniasis as the Only Method of Controling the Disease in the Enzootic Area in the Midwestern Minas Gerais","volume":"40","author":"Vaz","year":"2020","journal-title":"Pesqui. Vet. Bras."},{"key":"ref_23","first-page":"69","article-title":"Vaccines for Canine Leishmaniasis","volume":"3","year":"2012","journal-title":"Front. Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jim.2015.03.017","article-title":"Vaccines for visceral leishmaniasis: A review","volume":"422","author":"Jain","year":"2015","journal-title":"J. Immunol. Methods"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1111\/j.0105-2896.2004.00190.x","article-title":"Does the Leishmania major paradigm of pathogenesis and protection hold for new world cutaneous leishmaniases or the visceral disease?","volume":"201","author":"Alexander","year":"2004","journal-title":"Immunol. Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.vetpar.2019.05.006","article-title":"Canine visceral leishmaniasis biomarkers and their employment in vaccines","volume":"271","author":"Giunchetti","year":"2019","journal-title":"Vet. Parasitol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1128\/IAI.67.1.237-243.1999","article-title":"Compensation for decreased expression of B7 molecules on Leishmania infantum-infected canine macrophages results in restoration of parasite-specific T-cell proliferation and gamma interferon production","volume":"67","author":"Pinelli","year":"1999","journal-title":"Infect. Immun."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1002\/eji.1830250619","article-title":"Leishmania infantum-specific T cell lines derived from asymptomatic dogs that lyse infected macrophages in a major histocompatibility complex-restricted manner","volume":"25","author":"Pinelli","year":"1995","journal-title":"Eur. J. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.vetimm.2006.10.001","article-title":"Analysis of the cytokine profile in spleen cells from dogs naturally infected by Leishmania chagasi","volume":"115","author":"Lage","year":"2007","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.pt.2010.04.005","article-title":"Immunity to Leishmania and the rational search for vaccines against canine leishmaniasis","volume":"26","author":"Reis","year":"2010","journal-title":"Trends Parasitol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.jcpa.2011.03.001","article-title":"Cytokine gene expression in the tissues of dogs infected by Leishmania infantum","volume":"145","author":"Barbosa","year":"2011","journal-title":"J. Comp. Pathol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Gon\u00e7alves, A.A.M., Leite, J.C., Resende, L.A., Mariano, R.M.S., Silveira, P., Melo-J\u00fanior, O.A.O., Ribeiro, H.S., De Oliveira, D.S., Soares, D.F., and Santos, T.A.P. (2019). An Overview of Immunotherapeutic Approaches Against Canine Visceral Leishmaniasis: What Has Been Tested on Dogs and a New Perspective on Improving Treatment Efficacy. Front. Cell Infect. Microbiol., 9.","DOI":"10.3389\/fcimb.2019.00427"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4804","DOI":"10.1128\/IAI.71.8.4804-4807.2003","article-title":"Both interleukin-4 (IL-4) and IL-4 receptor alpha signaling contribute to the development of hepatic granulomas with optimal antileishmanial activity","volume":"71","author":"Alexander","year":"2003","journal-title":"Infect. Immun."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.vetpar.2014.08.022","article-title":"Immunological profile of resistance and susceptibility in naturally infected dogs by Leishmania infantum","volume":"205","author":"Leal","year":"2014","journal-title":"Vet. Parasitol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1111\/j.1365-3024.2006.00840.x","article-title":"Immunology of canine leishmaniasis","volume":"28","author":"Barbieri","year":"2006","journal-title":"Parasite Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.vetimm.2008.11.020","article-title":"Expression of IFN-gamma, TNF-alpha, IL-10 and TGF-beta in lymph nodes associates with parasite load and clinical form of disease in dogs naturally infected with Leishmania (Leishmania) chagasi","volume":"128","author":"Alves","year":"2009","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Do Nascimento, P.R., Martins, D.R., Monteiro, G.R., Queiroz, P.V., Freire-Neto, F.P., Queiroz, J.W., Lima, A.L.M., and Jeroimo, S.M.B. (2013). Association of pro-inflammatory cytokines and iron regulatory protein 2 (IRP2) with Leishmania burden in canine visceral leishmaniasis. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0073873"},{"key":"ref_38","first-page":"233","article-title":"Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation\/migration molecules in neutrophils and monocytes","volume":"15","author":"Moreira","year":"2016","journal-title":"Vet. Parasitol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1177\/0300985814521248","article-title":"Pathologic Mechanisms Underlying the Clinical Findings in Canine Leishmaniosis due to Leishmania infantum\/chagasi","volume":"51","author":"Koutinas","year":"2014","journal-title":"Vet. Pathol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1016\/j.vaccine.2008.11.094","article-title":"A prime\/boost DNA\/ Modified vaccinia virus Ankara vaccine expressing recombinant Leishmania DNA encoding TRYP is safe and immunogenic in outbred dogs, the reservoir of zoonotic visceral leishmaniasis","volume":"27","author":"Carson","year":"2009","journal-title":"Vaccine"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.vetpar.2018.07.011","article-title":"Comparative analysis of the Leishmania infantum-specific antibody repertoires and the autoantibody repertoires between asymptomatic and symptomatic dogs","volume":"15","author":"Chaabouni","year":"2018","journal-title":"Vet. Parasitol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.rvsc.2011.12.009","article-title":"Profile of anti-Leishmania antibodies related to clinical picture in canine visceral leishmaniasis","volume":"93","author":"Braga","year":"2012","journal-title":"Res. Vet. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1186\/s13071-016-1553-y","article-title":"Possibilities and challenges for developing a successful vaccine for leishmaniasis","volume":"9","author":"Srivastava","year":"2016","journal-title":"Parasit. Vectors"},{"key":"ref_44","unstructured":"Brasil Minist\u00e9rio da Agricultura e Pecu\u00e1ria (2023, July 31). Mapa Suspende Fabrica\u00e7\u00e3o e Venda e Determina o Recolhimento de lotes de Vacina Contra Leishmaniose, Available online: https:\/\/www.gov.br\/agricultura\/pt-br\/assuntos\/noticias\/mapa-suspende-fabricacao-e-venda-e-determina-o-recolhimento-de-lotes-de-vacina-contra-leishmaniose-apos-fiscalizacao."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1186\/s13071-022-05251-5","article-title":"Use of preventive measures and serological screening tools for Leishmania infantum infection in dogs from Europe","volume":"15","author":"Baxarias","year":"2022","journal-title":"Parasit. Vectors"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.1016\/j.vaccine.2016.03.019","article-title":"Field randomized trial to evaluate the efficacy of the Leish-Tec\u00ae vaccine against canine visceral leishmaniasis in an endemic area of Brazil","volume":"34","author":"Feres","year":"2016","journal-title":"Vaccine"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"5888","DOI":"10.1016\/j.vaccine.2008.05.095","article-title":"Protective immunity against challenge with Leishmania (Leishmania) chagasi in beagle dogs vaccinated with recombinant A2 protein","volume":"26","author":"Fernandes","year":"2008","journal-title":"Vaccine"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Grimaldi, G., Teva, A., Dos-Santos, C.B., Santos, F.N., Pinto, I.D.S., Fux, B., Leite, G.R., and Falqueto, A. (2017). Field trial of efficacy of the Leish-tec\u00ae vaccine against canine leishmaniasis caused by Leishmania infantum in an endemic area with high transmission rates. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0185438"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Aguiar-Soares, R.D.d.O., Roatt, B.M., Mathias, F.A.S., Reis, L.E.S., Cardoso, J.M.d.O., Brito, R.C.F., Ker, H.G., Corr\u00eaa-Oliveira, R., Giunchetti, R.C., and Reis, A.B. (2020). Phase I and II Clinical Trial Comparing the LBSap, Leishmune\u00ae, and Leish-Tec\u00ae Vaccines against Canine Visceral Leishmaniasis. Vaccines, 8.","DOI":"10.3390\/vaccines8040690"},{"key":"ref_50","unstructured":"European Medicines Agency (2022, May 18). LetiFend_: Summary of Product Characteristics. Available online: http:\/\/www.ema.europa.eu\/docs\/en_GB\/document_library\/EPAR_-_Summary_for_the_public\/veterinary\/003865\/WC500207742.pdf."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0165-2427(02)00315-X","article-title":"A Leishmania infantum multi-component antigenic protein mixed with live BCG confers protection to dogs experimentally infected with L. infantum","volume":"92","author":"Molano","year":"2003","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"5964","DOI":"10.1016\/j.vaccine.2009.07.069","article-title":"The Chimerical Multi-Component Q protein from Leishmania in the absence of adjuvant protects dogs against an experimental Leishmania infantum infection","volume":"27","author":"Iniesta","year":"2009","journal-title":"Vaccine"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.1016\/j.vaccine.2018.02.111","article-title":"A large-scale field randomized trial demonstrates safety and efficacy of the vaccine LetiFend\u00ae against canine leishmaniosis","volume":"36","author":"Cotrina","year":"2018","journal-title":"Vaccine"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Morales-Yuste, M., Mart\u00edn-S\u00e1nchez, J., and Corpas-Lopez, V. (2022). Canine Leishmaniasis: Update on Epidemiology, Diagnosis, Treatment, and Prevention. Vet. Sci., 9.","DOI":"10.3390\/vetsci9080387"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1007\/s00281-020-00788-y","article-title":"Leishmaniasis immunopathology\u2014Impact on design and use of vaccines, diagnostics and drugs","volume":"42","author":"Kaye","year":"2020","journal-title":"Semin. Immunopathol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"170","DOI":"10.2174\/1872208313666190114150511","article-title":"Recent Patents applications in red biotechnology: A mini-review","volume":"13","author":"Graciano","year":"2019","journal-title":"Recent. Pat. Biotechnol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/S0074-02762010000100001","article-title":"Transmission blocking vaccines to control insect-borne diseases\u2014A review","volume":"105","year":"2010","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.pt.2019.04.008","article-title":"Transmission-Blocking Vaccines for Malaria: Time to Talk about Vaccine Introduction","volume":"35","author":"Coelho","year":"2019","journal-title":"Trends Parasitol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"105104","DOI":"10.1016\/j.actatropica.2019.105104","article-title":"Genetic diversity and natural selection of transmission-blocking vaccine candidate antigens Pvs25 and Pvs28 in Plasmodium vivax Myanmar isolates","volume":"198","author":"Kang","year":"2019","journal-title":"Acta Trop."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1128\/iai.45.3.775-777.1984","article-title":"A surface protein expressed during the transformation of zygotes of Plasmodium gallinaceum is a target of transmission-blocking antibodies","volume":"45","author":"Grotendorst","year":"1984","journal-title":"Infect. Immun."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/S0264-410X(01)00402-9","article-title":"Antibodies to Plasmodium vivax transmission-blocking vaccine candidate antigens Pvs25 and Pvs28 do not show synergism","volume":"20","author":"Hisaeda","year":"2001","journal-title":"Vaccine"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"5228","DOI":"10.1073\/pnas.091447398","article-title":"Anti-mosquito midgut antibodies block development of Plasmodium falciparum and Plasmodium vivax in multiple species of Anopheles mosquitoes and reduce vector fecundity and survivorship","volume":"98","author":"Lal","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"6995","DOI":"10.1128\/IAI.71.12.6995-7001.2003","article-title":"Monoclonal antibody MG96 completely blocks Plasmodium yoelii development in Anopheles stephensi","volume":"71","author":"Dinglasan","year":"2003","journal-title":"Infect. Immun."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"13461","DOI":"10.1073\/pnas.0702239104","article-title":"Disruption of Plasmodium falciparum development by antibodies against a conserved mosquito midgut antigen","volume":"104","author":"Dinglasan","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1128\/IAI.00864-06","article-title":"Carboxypeptidases B of Anopheles gambiae as targets for a Plasmodium falciparum transmission-blocking vaccine","volume":"75","author":"Lavazec","year":"2007","journal-title":"Infect. Immun."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1365-2915.1990.tb00259.x","article-title":"Anti-mosquito antibodies reduce the susceptibility of Aedes aegypti to arbovirus infection","volume":"4","author":"Ramasamy","year":"1990","journal-title":"Med. Vet. Entomol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1111\/j.1365-2915.1990.tb00274.x","article-title":"Effect of anti-mosquito antibodies on the infectivity of the rodent malaria parasite Plasmodium berghei to Anopheles farauti","volume":"4","author":"Ramasamy","year":"1990","journal-title":"Med. Vet. Entomol."},{"key":"ref_68","first-page":"275","article-title":"Historical Perspective and Biotechnological Trends to Block Arboviruses Transmission by Controlling Aedes aegypti Mosquitos Using Different Approaches","volume":"23","author":"Silveira","year":"2020","journal-title":"Front. Med."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1186\/s13071-016-1802-0","article-title":"Arbovirosis and potential transmission blocking vaccines","volume":"9","author":"Troupin","year":"2016","journal-title":"Parasites Vectors"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0001-706X(95)00132-X","article-title":"The effect of immune sera from hamsters immunized with sandfly gut and whole body extract antigens on the fecundity and mortality of Phlebotomus duboscqi (Diptera: Psychodidae)","volume":"60","author":"Ingonga","year":"1996","journal-title":"Acta Trop."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"84","DOI":"10.4314\/eamj.v78i2.9094","article-title":"Transmission Blocking Vaccine Studies in Leishmaniasis: I. Lipophosphoglycan Is a Promising Transmission Blocking Vaccine Molecule against Cutaneous Leishmaniasis","volume":"78","author":"Tonui","year":"2001","journal-title":"East. Afr. Med. J."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.cell.2004.10.009","article-title":"A Role for Insect Galectins in Parasite Survival","volume":"119","author":"Kamhawi","year":"2004","journal-title":"Cell"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1590\/S1519-69842006000100022","article-title":"Considerations on the effect of anti-sandfly antibodies on biological parameters of Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae: Phlebotominae)","volume":"66","author":"Vilela","year":"2006","journal-title":"Braz. J. Biol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"2423","DOI":"10.1016\/j.vaccine.2005.11.061","article-title":"The FML-vaccine (Leishmune\u00ae) against canine visceral leishmaniasis: A transmission blocking vaccine","volume":"24","author":"Saraiva","year":"2006","journal-title":"Vaccine"},{"key":"ref_75","unstructured":"MAPA (Minist\u00e9rio da Agricultura, Pecu\u00e1ria e Abastecimento) (2022, May 14). Nota t\u00e9cnica n\u00b0038\/2014\/DFIP\/DAS. Suspens\u00e3o da Licen\u00e7a de Fabrica\u00e7\u00e3o e Comercializa\u00e7\u00e3o do Produto Leishmune Vacina Contra Leishmanioses Visceral Canina. Bras\u00edlia, Available online: www.agricultura.gov.br\/assuntos\/politica-agricola\/arquivos\/notatecnica-dfip-38-14-leishmune.pdf\/view."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Coutinho-Abreu, I.V., Sharma, N.K., Robles-Murguia, M., and Ramalho-Ortigao, M. (2010). Targeting the Midgut Secreted Ppchit1 Reduces Leishmania Major Development in Its Natural Vector, the Sand Fly Phlebotomus Papatasi. PLoS Neglected Trop. Dis., 4.","DOI":"10.1371\/journal.pntd.0000901"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/j.vetpar.2013.05.008","article-title":"Vaccination with LiESP\/QA-21 (CaniLeish\u00ae) Reduces the Intensity of Infection in Phlebotomus Perniciosus Fed on Leishmania Infantum Infected Dogs-A Preliminary Xenodiagnosis Study","volume":"197","author":"Bongiorno","year":"2013","journal-title":"Vet. Parasitol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.molimm.2022.05.118","article-title":"How to get away with murder: The multiple strategies employed by pathogenic protozoa to avoid complement killing","volume":"149","author":"Horta","year":"2022","journal-title":"Mol. Immunol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1111\/j.1462-5822.2010.01439.x","article-title":"Proteophosphoglycan confers resistance of Leishmania major to midgut digestive enzymes induced by blood feeding in vector sand flies","volume":"12","author":"Secundino","year":"2010","journal-title":"Cell. Microbiol."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Quintela-Carvalho, G., Goicochea, A.M.C., Man\u00e7ur-Santos, V., Viana, S.M., Luz, Y.D.S., Dias, B.R.S., L\u00e1zaro-Souza, M., Suarez, M., de Oliveira, C.I., and Saraiva, E.M. (2022). Leishmania infantum Defective in Lipophosphoglycan Biosynthesis Interferes with Activation of Human Neutrophils. Front. Cell Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.788196"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"e200140","DOI":"10.1590\/0074-02760200140","article-title":"Lipophosphoglycans from dermotropic Leishmania infantum are more pro-inflammatory than those from viscerotropic strains","volume":"115","author":"Cardoso","year":"2020","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/s41541-019-0144-2","article-title":"Peptide-based vaccine successfully induces protective immunity against canine visceral leishmaniasis","volume":"4","author":"Petitdidier","year":"2019","journal-title":"NPJ Vaccines"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1051\/parasite\/2017041","article-title":"Laboratory colonization and mass rearing of phlebotomine sand flies (Diptera, Psychodidae)","volume":"24","author":"Lawyer","year":"2017","journal-title":"Parasite"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"101631","DOI":"10.1016\/j.ttbdis.2020.101631","article-title":"Vaccine approaches applied to controlling dog ticks","volume":"12","author":"Ribeiro","year":"2021","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_85","unstructured":"Giunchetti, R.C., Filho, O.A.M., Mendon\u00e7a, L.Z., Gontijo, N.F., Bartholomeu, D.C., and Borges, W.C. (2013). Imunobiol\u00f3gico para Controle do Vetor da Leishmaniose, Processos de Obten\u00e7\u00e3o e uso. (BR1020130228052)."}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/11\/10\/1565\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:01:26Z","timestamp":1760130086000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/11\/10\/1565"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,5]]},"references-count":85,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["vaccines11101565"],"URL":"https:\/\/doi.org\/10.3390\/vaccines11101565","relation":{},"ISSN":["2076-393X"],"issn-type":[{"value":"2076-393X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,5]]}}}