{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:34:37Z","timestamp":1760150077256,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T00:00:00Z","timestamp":1697846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008771","name":"Animal and Plant Quarantine Agency, Republic of Korea","doi-asserted-by":"publisher","award":["B-1543083-2023-25-02"],"award-info":[{"award-number":["B-1543083-2023-25-02"]}],"id":[{"id":"10.13039\/501100008771","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>A chimeric pestivirus (KD26_E2LOM) was prepared by inserting the E2 gene of the classical swine fever virus (CSFV) LOM strain into the backbone of the bovine viral diarrhea virus (BVDV) KD26 strain. KD26_E2LOM was obtained by transfecting the cDNA pACKD26_E2LOM into PK-15 cells. KD26_E2LOM chimeric pestivirus proliferated to titers of 106.5 TCID50\/mL and 108.0 TCID50\/mL at 96 h post-inoculation into PK-15 cells or MDBK cells, respectively. It also reacted with antibodies specific for CSFV E2 and BVDV Erns, but not with an anti-BVDV E2 antibody. Piglets (55\u201360 days old) inoculated with a high dose (107.0 TCID50\/mL) of KD26_E2LOM produced high levels of CSFV E2 antibodies. In addition, no co-habiting pigs were infected with KD26_E2LOM; however, some inoculated pigs excreted the virus, and the virus was detected in some organs. When pregnant sows were inoculated during the first trimester (55\u201360 days) with a high dose (107.0 TCID50\/mL) of KD26_E2LOM, anti-CSFV E2 antibodies were produced at high levels; chimeric pestivirus was detected in one fetus and in the ileum of one sow. When 5-day-old calves that did not consume colostrum received a high dose (107.0 TCID50\/mL) of KD26_E2LOM, one calf secreted the virus in both feces and nasal fluid on Day 2. A high dose of KD26_E2LOM does not induce specific clinical signs in most animals, does not spread from animal to animal, and generates CSFV E2 antibodies with DVIA functions. Therefore, chimeric pestivirus KD26_E2LOM is a potential CSFV live marker vaccine.<\/jats:p>","DOI":"10.3390\/vaccines11101622","type":"journal-article","created":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T12:59:01Z","timestamp":1697893141000},"page":"1622","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Safety and Immunogenicity of Chimeric Pestivirus KD26_E2LOM in Piglets and Calves"],"prefix":"10.3390","volume":"11","author":[{"given":"Gyu-Nam","family":"Park","sequence":"first","affiliation":[{"name":"Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7707-3861","authenticated-orcid":false,"given":"Jihye","family":"Shin","sequence":"additional","affiliation":[{"name":"Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SeEun","family":"Choe","sequence":"additional","affiliation":[{"name":"Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ki-Sun","family":"Kim","sequence":"additional","affiliation":[{"name":"Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8013-6834","authenticated-orcid":false,"given":"Jae-Jo","family":"Kim","sequence":"additional","affiliation":[{"name":"Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong-In","family":"Lim","sequence":"additional","affiliation":[{"name":"Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byung-Hyun","family":"An","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Seoul University, Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bang-Hun","family":"Hyun","sequence":"additional","affiliation":[{"name":"Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5690-4039","authenticated-orcid":false,"given":"Dong-Jun","family":"An","sequence":"additional","affiliation":[{"name":"Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Blome, S., Staubach, C., Henke, J., Carlson, J., and Beer, M. 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