{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T06:54:40Z","timestamp":1774853680675,"version":"3.50.1"},"reference-count":61,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,30]],"date-time":"2024-01-30T00:00:00Z","timestamp":1706572800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PATENT CO. DOO","award":["nr73380"],"award-info":[{"award-number":["nr73380"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Maintaining respiratory tract health is crucial for layers, impacting gut health, laying performance, and egg quality. Viral diseases and standard vaccinations can compromise tracheal epithelium function, leading to oxidative stress. This study assessed the impact of a blend of feed additives, predominantly lysozyme (L), essential oils (EO), and vitamins (VIT) (referred to as L + EO + VIT), on young layers during an oral vaccination schedule. The supplementation significantly enhanced antibody titers for Newcastle Disease Virus (NDV) and Infectious Bronchitis Virus (IBV) after vaccination, trachea functionality and intestinal health in the jejunum, increased egg production, and exhibited a trend toward higher egg weight. Although feed intake showed no significant difference, egg quality remained consistent across experimental groups. Moreover, L + EO + VIT supplementation elevated total phenolic content in eggs, improving oxidative stability in both fresh and stored eggs, particularly under iron-induced oxidation. Notably, it substantially reduced yolk lipid peroxidation and albumen protein carbonyls. In conclusion, water supplementation with L + EO + VIT may enhance humoral immune response to IBV and NDV, positively impacting hen productivity. These findings indicate improved tracheal function and enhanced oxidative stability, emphasizing the potential of this blend in promoting overall health and performance in layers.<\/jats:p>","DOI":"10.3390\/vaccines12020147","type":"journal-article","created":{"date-parts":[[2024,1,30]],"date-time":"2024-01-30T11:35:34Z","timestamp":1706614534000},"page":"147","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of Dietary Supplementation of Essential Oils, Lysozyme, and Vitamins\u2019 Blend on Layer Hen Performance, Viral Vaccinal Response, and Egg Quality Characteristics"],"prefix":"10.3390","volume":"12","author":[{"given":"Tilemachos","family":"Mantzios","sequence":"first","affiliation":[{"name":"Unit of Avian Medicine, Clinic of Farm Animals, Aristotle University of Thessaloniki, Stavrou Voutyra 11, 54627 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ioanna","family":"Stylianaki","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, Faculty of Veterinary Medicine, Aristotle University, 54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6086-1097","authenticated-orcid":false,"given":"Soumela","family":"Savvidou","sequence":"additional","affiliation":[{"name":"Research Institute of Animal Science, Hellenic Agricultural Organisation-Demeter, 58100 Giannitsa, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0181-8416","authenticated-orcid":false,"given":"Stella","family":"Dokou","sequence":"additional","affiliation":[{"name":"Laboratory of Nutrition, Faculty of Veterinary Medicine, Aristotle University, 54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0958-4121","authenticated-orcid":false,"given":"Georgios \u0391.","family":"Papadopoulos","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Husbandry, Faculty of Veterinary Medicine, Aristotle University, 54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3296-4050","authenticated-orcid":false,"given":"Ioannis","family":"Panitsidis","sequence":"additional","affiliation":[{"name":"Laboratory of Nutrition, Faculty of Veterinary Medicine, Aristotle University, 54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2936-2083","authenticated-orcid":false,"given":"Apostolos","family":"Patsias","sequence":"additional","affiliation":[{"name":"Agricultural Poultry Cooperation of Ioannina \u201cPINDOS\u201d, Rodotopi, 45500 Ioannina, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4408-255X","authenticated-orcid":false,"given":"Jog","family":"Raj","sequence":"additional","affiliation":[{"name":"PATENT CO. 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(2011). Improving the Safety and Quality of Eggs and Egg Products, Woodhead Publishing."},{"key":"ref_4","first-page":"1","article-title":"Respiratory Diseases in Poultry: A Constant Challenge Mini Review","volume":"4","author":"Furian","year":"2018","journal-title":"Approaches Poult. Dairy Vet. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.vetmic.2016.12.019","article-title":"Newcastle Disease Vaccines\u2014A Solved Problem or a Continuous Challenge?","volume":"206","author":"Dimitrov","year":"2017","journal-title":"Vet. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1038\/ismej.2007.31","article-title":"The Effects of Subtherapeutic Antibiotic Use in Farm Animals on the Proliferation and Persistence of Antibiotic Resistance among Soil Bacteria","volume":"1","author":"Ghosh","year":"2007","journal-title":"ISME J."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Abreu, R., Semedo-Lemsaddek, T., Cunha, E., Tavares, L., and Oliveira, M. (2023). Antimicrobial Drug Resistance in Poultry Production: Current Status and Innovative Strategies for Bacterial Control. Microorganisms, 11.","DOI":"10.3390\/microorganisms11040953"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Stevanovi\u0107, Z.D., Bo\u0161njak-Neum\u00fcller, J., Paji\u0107-Lijakovi\u0107, I., Raj, J., and Vasiljevi\u0107, M. (2018). Essential Oils as Feed Additives\u2014Future Perspectives. Molecules, 23.","DOI":"10.3390\/molecules23071717"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1080\/00071668.2018.1483577","article-title":"Effect of Herbal Feed Additives on Performance Parameters, Intestinal Microbiota, Intestinal Morphology and Meat Lipid Oxidation of Broiler Chickens","volume":"59","author":"Giannenas","year":"2018","journal-title":"Br. Poult. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.14737\/journal.aavs\/2014.2.1.1.7","article-title":"Essential Oils as a Feed Additive in Poultry Nutrition","volume":"2","author":"Gopi","year":"2014","journal-title":"Adv. Anim. Vet. Sci."},{"key":"ref_11","first-page":"231","article-title":"The Effect of Feeding Thyme, Sage and Rosemary Oil on Laying Hen Performance, Cholesterol and Some Proteins Ratio of Egg Yolk and Escherichia Coli Count in Feces","volume":"72","author":"Erhan","year":"2008","journal-title":"Arch. Fur Geflugelkd."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1515\/aoas-2016-0038","article-title":"The Effect of Dietary Essential Oil Mixture Supplementation on Performance, Egg Quality and Bone Characteristics in Laying Hens","volume":"16","author":"Olgun","year":"2016","journal-title":"Ann. Anim. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.aninu.2017.03.005","article-title":"Effects of Essential Oils on Performance, Egg Quality, Nutrient Digestibility and Yolk Fatty Acid Profile in Laying Hens","volume":"3","author":"Ding","year":"2017","journal-title":"Anim. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Marchese, A., Arciola, C.R., Barbieri, R., Silva, A.S., Nabavi, S.F., Tsetegho Sokeng, A.J., Izadi, M., Jafari, N.J., Suntar, I., and Daglia, M. (2017). Update on Monoterpenes as Antimicrobial Agents: A Particular Focus on p-Cymene. Materials, 10.","DOI":"10.3390\/ma10080947"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"866","DOI":"10.3923\/ijps.2005.866.871","article-title":"Oregano Herb Versus Oregano Essential Oil as Feed Supplements to Increase the Oxidative Stability of Turkey Meat","volume":"4","author":"Palatos","year":"2005","journal-title":"Int. J. Poult. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Dilawar, M.A., Mun, H.S., Rathnayake, D., Yang, E.J., Seo, Y.S., Park, H.S., and Yang, C.J. (2021). Egg Quality Parameters, Production Performance and Immunity of Laying Hens Supplemented with Plant Extracts. Animals, 11.","DOI":"10.3390\/ani11040975"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1111\/jpn.13869","article-title":"The Role of Phytogenic Feed Additives in Stress Mitigation in Broiler Chickens","volume":"108","author":"Oni","year":"2024","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Memar, M.Y., Raei, P., Alizadeh, N., Akbari Aghdam, M., and Kafil, H.S. (2017). Carvacrol and Thymol: Strong Antimicrobial Agents against Resistant Isolates. Rev. Res. Med. Microbiol., 28.","DOI":"10.1097\/MRM.0000000000000100"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"225","DOI":"10.5713\/ajas.2013.13376","article-title":"Dietary Supplementation of Benzoic Acid and Essential Oil Compounds Affects Buffering Capacity of the Feeds, Performance of Turkey Poults and Their Antioxidant Status, pH in the Digestive Tract, Intestinal Microbiota and Morphology","volume":"27","author":"Giannenas","year":"2014","journal-title":"Asian-Australas J. Anim. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"103916","DOI":"10.1016\/j.micpath.2019.103916","article-title":"Combination of Herbal Components (Curcumin, Carvacrol, Thymol, Cinnamaldehyde) in Broiler Chicken Feed: Impacts on Response Parameters, Performance, Fatty Acid Profiles, Meat Quality and Control of Coccidia and Bacteria","volume":"139","author":"Galli","year":"2020","journal-title":"Microb. Pathog."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1080\/03079450903447404","article-title":"Exogenous Lysozyme Influences Clostridium Perfringens Colonization and Intestinal Barrier Function in Broiler Chickens","volume":"39","author":"Liu","year":"2010","journal-title":"Avian Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5059","DOI":"10.1016\/S0021-9258(17)37654-8","article-title":"Enhanced Bactericidal Action of Lysozyme to Escherichia Coli by Inserting a Hydrophobic Pentapeptide into Its C Terminus","volume":"269","author":"Ibrahim","year":"1994","journal-title":"J. Biol. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1637\/9370-041410-ResNote.1","article-title":"Effect of a Radiant Energy\u2013Treated Lysozyme Antimicrobial Blend on the Control of Clostridial Necrotic Enteritis in Broiler Chickens","volume":"54","author":"Zhang","year":"2010","journal-title":"Avian Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0304-4165(00)00041-6","article-title":"The Mode of Actions of Lysozyme as an Immunoglobulin Production Stimulating Factor","volume":"1475","author":"Sugahara","year":"2000","journal-title":"Biochim. Et Biophys. Acta (BBA)-Gen. Subj."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Dom\u00ednguez-Bello, M.G., Pacheco, M.A., Ruiz, M.C., Michelangeli, F., Leippe, M., and de Pedro, M.A. (2004). Resistance of Rumen Bacteria Murein to Bovine Gastric Lysozyme. BMC Ecol., 4.","DOI":"10.1186\/1472-6785-4-7"},{"key":"ref_26","unstructured":"Suarez, D.L., Miller, P.J., Koch, G., Mundt, E., and Rautenschlein, S. (2020). Diseases of Poultry, John Wiley & Sons, Ltd."},{"key":"ref_27","unstructured":"Jackwood, M.W., and de Wit, S. (2013). Diseases of Poultry, John Wiley & Sons, Ltd."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1111\/j.1365-2621.1999.tb15870.x","article-title":"Volatiles Production and Lipid Oxidation in Irradiated Cooked Sausage as Related to Packaging and Storage","volume":"64","author":"Ahn","year":"1999","journal-title":"J. Food Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1007\/BF02533546","article-title":"Relative Susceptibility of Microsomes from Lung, Heart, Liver, Kidney, Brain and Testes to Lipid Peroxidation: Correlation with Vitamin E Content","volume":"15","author":"Kornbrust","year":"1980","journal-title":"Lipids"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.neulet.2003.10.080","article-title":"Thiol Redox State (TRS) and Oxidative Stress in the Mouse Hippocampus after Pentylenetetrazol-Induced Epileptic Seizure","volume":"357","author":"Patsoukis","year":"2004","journal-title":"Neurosci. Lett."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1006\/abio.1999.4019","article-title":"Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E","volume":"269","author":"Prieto","year":"1999","journal-title":"Anal. Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1016\/j.ijbiomac.2019.10.234","article-title":"Effects of Inulin Supplementation in Laying Hens Diet on the Antioxidant Capacity of Refrigerated Stored Eggs","volume":"153","author":"Shang","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1590\/1516-635x1702145-150","article-title":"Determining the Best Sectioning Method and Intestinal Segment for Morphometric Analysis in Broilers","volume":"17","author":"Gava","year":"2015","journal-title":"Braz. J. Poult. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1016\/S0041-1345(00)01238-0","article-title":"An Evaluation of Methods for Grading Histologic Injury Following Ischemia\/Reperfusion of the Small Bowel","volume":"32","author":"Quaedackers","year":"2000","journal-title":"Transplant. Proc."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1637\/6040","article-title":"Evaluation of the Protection Conferred by Commercial Vaccines Against the California 99 Isolate of Infectious Bronchitis Virus","volume":"47","author":"Alvarado","year":"2003","journal-title":"Avian Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.3382\/ps.2011-02023","article-title":"Performance, Egg Quality, and Immune Response of Laying Hens Fed Diets Supplemented with Mannan-Oligosaccharide or an Essential Oil Mixture under Moderate and Hot Environmental Conditions","volume":"91","author":"Bozkurt","year":"2012","journal-title":"Poult. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"100755","DOI":"10.1016\/j.animal.2023.100755","article-title":"Review: Current Challenges in Poultry Nutrition, Health, and Welfare","volume":"17","author":"Korver","year":"2023","journal-title":"Animal"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"278","DOI":"10.3382\/ps.2010-00962","article-title":"Hen Welfare in Different Housing Systems1","volume":"90","author":"Lay","year":"2011","journal-title":"Poult. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1111\/jam.12334","article-title":"Modulation by Essential Oil of Vaccine Response and Production Improvement in Chicken Challenged with Velogenic Newcastle Disease Virus","volume":"115","author":"Barbour","year":"2013","journal-title":"J. Appl. Microbiol."},{"key":"ref_40","unstructured":"De Wit, J. (2013, January 19\u201323). Underestimation of the Difficulties of Vaccination against Viral Respiratory Diseases by Mass Application Methods. Proceedings of the XVIIIth International Congress of the World Veterinary Poultry Association, Nantes, France."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/S0034-5288(18)32889-3","article-title":"Comparison of Duration of Immunity in Chickens Infected with a Live Infectious Bronchitis Vaccine by Three Different Routes","volume":"26","author":"Gough","year":"1979","journal-title":"Res. Vet. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Abo Ghanima, M.M., Elsadek, M.F., Taha, A.E., Abd El-Hack, M.E., Alagawany, M., Ahmed, B.M., Elshafie, M.M., and El-Sabrout, K. (2020). Effect of Housing System and Rosemary and Cinnamon Essential Oils on Layers Performance, Egg Quality, Haematological Traits, Blood Chemistry, Immunity, and Antioxidant. Animals, 10.","DOI":"10.3390\/ani10020245"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"957847","DOI":"10.3389\/fvets.2022.957847","article-title":"The Association of Curcuma and Scutellaria Plant Extracts Improves Laying Hen Thermal Tolerance and Egg Oxidative Stability and Quality under Heat Stress Conditions","volume":"9","author":"Giannenas","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Sidiropoulou, E., Marug\u00e1n-Hern\u00e1ndez, V., Skoufos, I., Giannenas, I., Bonos, E., Aguiar-Martins, K., Lazari, D., Papagrigoriou, T., Fotou, K., and Grigoriadou, K. (2022). In Vitro Antioxidant, Antimicrobial, Anticoccidial, and Anti-Inflammatory Study of Essential Oils of Oregano, Thyme, and Sage from Epirus, Greece. Life, 12.","DOI":"10.3390\/life12111783"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1080\/10412905.2016.1153002","article-title":"Beneficial Impacts of Thymol Essential Oil on Health and Production of Animals, Fish and Poultry: A Review","volume":"28","author":"Alagawany","year":"2016","journal-title":"J. Essent. Oil Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"423","DOI":"10.3109\/13880209.2014.923003","article-title":"Evaluation of P-Cymene, a Natural Antioxidant","volume":"53","year":"2015","journal-title":"Pharm. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"128468","DOI":"10.1016\/j.foodchem.2020.128468","article-title":"Synergic Antioxidant Activity of \u03b3-Terpinene with Phenols and Polyphenols Enabled by Hydroperoxyl Radicals","volume":"345","author":"Guo","year":"2021","journal-title":"Food Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.foodcont.2015.09.033","article-title":"Antioxidant Properties of Coriander Essential Oil and Linalool and Their Potential to Control Campylobacter spp.","volume":"61","author":"Duarte","year":"2016","journal-title":"Food Control"},{"key":"ref_49","unstructured":"Florou-Paneri, P., Christaki, E., and Giannenas, I. (2019). Feed Additives: Aromatic Plants and Herbs in Animal Nutrition and Health, Academic Press."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Abdel-Latif, M.A., El-Far, A.H., Elbestawy, A.R., Ghanem, R., Mousa, S.A., and El-Hamid, H.S.A. (2017). Exogenous Dietary Lysozyme Improves the Growth Performance and Gut Microbiota in Broiler Chickens Targeting the Antioxidant and Non-Specific Immunity mRNA Expression. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0185153"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1017\/S0043933910000310","article-title":"Antioxidant Properties of Vitamins in Nutrition of Broiler Breeders and Laying Hens","volume":"66","author":"Rocha","year":"2010","journal-title":"World\u2019s Poult. Sci. J."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Obianwuna, U.E., Oleforuh-Okoleh, V.U., Wang, J., Zhang, H.-J., Qi, G.-H., Qiu, K., and Wu, S.-G. (2022). Potential Implications of Natural Antioxidants of Plant Origin on Oxidative Stability of Chicken Albumen during Storage: A Review. Antioxidants, 11.","DOI":"10.3390\/antiox11040630"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1111\/j.1365-2621.1994.tb05561.x","article-title":"Oxidative Stability and Sensory Quality of Stored Eggs From Hens Fed 1.5% Menhaden Oil","volume":"59","author":"Marshall","year":"1994","journal-title":"J. Food Sci."},{"key":"ref_54","unstructured":"(2023, December 02). VM174\/VM136: Investigating Vaccination Failure in Poultry Flocks. Available online: https:\/\/edis.ifas.ufl.edu\/publication\/VM136."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Bhuiyan, M.S.A., Amin, Z., Bakar, A.M.S.A., Saallah, S., Yusuf, N.H.M., Shaarani, S.M., and Siddiquee, S. (2021). Factor Influences for Diagnosis and Vaccination of Avian Infectious Bronchitis Virus (Gammacoronavirus) in Chickens. Vet. Sci., 8.","DOI":"10.3390\/vetsci8030047"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"833618","DOI":"10.3389\/fnut.2022.833618","article-title":"An Overview of Antimicrobial Activity of Lysozyme and Its Functionality in Cheese","volume":"9","author":"Khorshidian","year":"2022","journal-title":"Front. Nutr."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Ferraboschi, P., Ciceri, S., and Grisenti, P. (2021). Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic. Antibiotics, 10.","DOI":"10.3390\/antibiotics10121534"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Ragland, S.A., and Criss, A.K. (2017). From Bacterial Killing to Immune Modulation: Recent Insights into the Functions of Lysozyme. PLoS Pathog., 13.","DOI":"10.1371\/journal.ppat.1006512"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Khalil, K., Islam, M., Sujan, K., Mustari, A., Ahmad, N., and Miah, M. (2020). Dietary Acidifier and Lysozyme Improve Growth Performances and Hemato-Biochemical Profile in Broiler Chicken. J. Adv. Biotechnol. Exp. Ther., 3.","DOI":"10.5455\/jabet.2020.d130"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Bello, M.B., Yusoff, K., Ideris, A., Hair-Bejo, M., Peeters, B.P.H., and Omar, A.R. (2018). Diagnostic and Vaccination Approaches for Newcastle Disease Virus in Poultry: The Current and Emerging Perspectives. BioMed Res. Int., 2018.","DOI":"10.1155\/2018\/7278459"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1637\/10043-122011-Reg.1","article-title":"Characterization of Live LaSota Vaccine Strain\u2013Induced Protection in Chickens upon Early Challenge with a Virulent Newcastle Disease Virus of Heterologous Genotype","volume":"56","author":"Cornax","year":"2012","journal-title":"Avian Dis."}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/12\/2\/147\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:51:53Z","timestamp":1760104313000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/12\/2\/147"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,30]]},"references-count":61,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["vaccines12020147"],"URL":"https:\/\/doi.org\/10.3390\/vaccines12020147","relation":{},"ISSN":["2076-393X"],"issn-type":[{"value":"2076-393X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,30]]}}}