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Council","award":["0273"],"award-info":[{"award-number":["0273"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Age alters the host\u2019s susceptibility to immune induction. Humoral immunity with circulating antibodies, particularly immunoglobulin G (IgG), plays an essential role in immune response. IgG glycosylation in the fragment crystallizable (Fc) region, including sialylation, is important in regulating the effector function by interacting with Fc gamma receptors (Fc\u03b3Rs). Glycosylation is fundamentally changed with age and inflammatory responses. We aimed to explore the regulation of humoral immunity by comparing responses to antigen-induced immune challenges in young and adult mice using a local antigen-induced arthritis mouse model. This study examines the differences in immune response between healthy and immune-challenged states across these groups. Our initial assessment of the arthritis model indicated that adult mice presented more severe knee swelling than their younger counterparts. In contrast, we found that neither histological assessment, bone mineral density, nor the number of osteoclasts differs. Our data revealed an age-associated but not immune challenge increase in total IgG; the only subtype affected by immune challenge was IgG1 and partially IgG3. Interestingly, the sialylation of IgG2b and IgG3 is affected by age and immune challenges but not stimulated further by immune challenges in adult mice. This suggests a shift in IgG towards a pro-inflammatory and potentially pathogenic state with age and inflammation.<\/jats:p>","DOI":"10.3390\/vaccines12050479","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T08:02:22Z","timestamp":1714723342000},"page":"479","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Local Immune Activation and Age Impact on Humoral Immunity in Mice, with a Focus on IgG Sialylation"],"prefix":"10.3390","volume":"12","author":[{"given":"Priti","family":"Gupta","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, 413 90 Gothenburg, Sweden"},{"name":"Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Osteoporosis Centre and Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, 413 45 Gothenburg, Sweden"},{"name":"SciLifeLab, University of Gothenburg, 413 90 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5835-3912","authenticated-orcid":false,"given":"Tibor","family":"S\u00e1ghy","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, 413 90 Gothenburg, Sweden"},{"name":"Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Osteoporosis Centre and Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, 413 45 Gothenburg, Sweden"},{"name":"SciLifeLab, University of Gothenburg, 413 90 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1639-8079","authenticated-orcid":false,"given":"Miriam","family":"Bollmann","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, 413 90 Gothenburg, Sweden"},{"name":"SciLifeLab, University of Gothenburg, 413 90 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9039-0628","authenticated-orcid":false,"given":"Tao","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, 413 90 Gothenburg, Sweden"},{"name":"Department of Rheumatology, Sahlgrenska University Hospital, 413 46 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claes","family":"Ohlsson","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Osteoporosis Centre and Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, 413 45 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Carlsten","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, 413 90 Gothenburg, Sweden"},{"name":"Department of Rheumatology, Sahlgrenska University Hospital, 413 46 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1865-3494","authenticated-orcid":false,"given":"Carmen","family":"Corciulo","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, 405 30 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8914-0178","authenticated-orcid":false,"given":"Cecilia","family":"Engdahl","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, 413 90 Gothenburg, Sweden"},{"name":"Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Osteoporosis Centre and Centre for Bone and Arthritis Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, 413 45 Gothenburg, Sweden"},{"name":"SciLifeLab, University of Gothenburg, 413 90 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Forthal, D.N. 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