{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:17:11Z","timestamp":1760145431364,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T00:00:00Z","timestamp":1722297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Project of China","award":["2021YFC2302405","2022B1111020002","SKLPBS2218"],"award-info":[{"award-number":["2021YFC2302405","2022B1111020002","SKLPBS2218"]}]},{"name":"Key-Area Research and Development Program of Guangdong Province","award":["2021YFC2302405","2022B1111020002","SKLPBS2218"],"award-info":[{"award-number":["2021YFC2302405","2022B1111020002","SKLPBS2218"]}]},{"name":"foundation of State Key Laboratory of Pathogen and Biosecurity","award":["2021YFC2302405","2022B1111020002","SKLPBS2218"],"award-info":[{"award-number":["2021YFC2302405","2022B1111020002","SKLPBS2218"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Yellow fever (YF), caused by the yellow fever virus (YFV), continually spreads and causes epidemics worldwide, posing a great threat to human health. The live-attenuated YF 17D vaccine (YF-17D) has been licensed for preventing YFV infection and administrated via the intramuscular (i.m.) route. In this study, we sought to determine the immunogenicity and protective efficacy of aerosolized YF-17D via the intratracheal (i.t.) route in mice. YF-17D stocks in liquids were successfully aerosolized into particles of 6 \u03bcm. Further in vitro phenotype results showed the aerosolization process did not abolish the infectivity of YF-17D. Meanwhile, a single i.t. immunization with aerosolized YF-17D induced robust humoral and cellular immune responses in A129 mice, which is comparable to that received i.p. immunization. Notably, the aerosolized YF-17D also triggered specific secretory IgA (SIgA) production in bronchoalveolar lavage. Additionally, all immunized animals survived a lethal dose of YFV challenge in mice. In conclusion, our results support further development of aerosolized YF-17D in the future.<\/jats:p>","DOI":"10.3390\/vaccines12080856","type":"journal-article","created":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T08:02:27Z","timestamp":1722412947000},"page":"856","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Immunogenicity and Protective Efficacy of Aerosolized Live-Attenuated Yellow Fever 17D Vaccine in Mice"],"prefix":"10.3390","volume":"12","author":[{"given":"Feng","family":"Zhu","sequence":"first","affiliation":[{"name":"School of Life Sciences, Southwest Forestry University, Kunming 650224, China"},{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng-Xu","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suo-Qun","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0632-2807","authenticated-orcid":false,"given":"Cheng-Feng","family":"Qin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Hua","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Southwest Forestry University, Kunming 650224, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-Qiang","family":"Deng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Hansen, C.A., and Barrett, A.D.T. 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