{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:09:21Z","timestamp":1792591761721,"version":"4.5.0"},"reference-count":42,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,11,30]],"date-time":"2024-11-30T00:00:00Z","timestamp":1732924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["075-15-2021-1049"],"award-info":[{"award-number":["075-15-2021-1049"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Background\/Objectives: IgA1 protease is one of the virulence factors of Neisseria meningitidis, Haemophilus influenzae and other pathogens causing bacterial meningitis. The aim of this research is to create recombinant proteins based on fragments of the mature IgA1 protease A28\u2013P1004 from N.\u00a0meningitidis serogroup B strain H44\/76. These proteins are potential components of an antimeningococcal vaccine for protection against infections caused by pathogenic strains of N.\u00a0meningitidis and other bacteria producing serine-type IgA1 proteases. Methods: To obtain promising antigens for creating a vaccine, we designed and obtained several recombinant proteins. These proteins consisted of single or directly connected fragments selected from various regions of the IgA1 protease A28\u2013P1004. The choice of these fragments was based on our calculated data on the distribution of linear and conformational B-cell epitopes and MHC-II T-cell epitopes in the structure of IgA1 protease, taking into account the physicochemical properties of potential compounds and the results of a comparative analysis of the spatial structures of the original IgA1 protease and potential recombinant proteins. We studied the immunogenic and protective effects of the obtained proteins on the BALB\/c mice against meningococci of serogroups A, B and C. Results: Proteins MA28\u2013P1004-LEH6, MW140\u2013K833-LEH6, MW329\u2013P1004-LEH6, M(W140\u2013H328)-(W412\u2013D604)-(Y866\u2013P1004)-LEH6 and M(W140\u2013Q299)-(Y866\u2013P1004)-LEH6 have shown the following antibody titers, 103\/titer: 11 \u00b1 1, 6 \u00b1 2, 6 \u00b1 1, 9 \u00b1 1 and 22 \u00b1 3, respectively. Also, the last two proteins have shown the best average degree of protection from N.\u00a0meningitidis serogroups A, B and C, %: 62 \u00b1 6, 63 \u00b1 5, 67 \u00b1 4 respectively for M(W140\u2013H328)-(W412\u2013D604)-(Y866\u2013P1004)-LEH6 and 70 \u00b1 5, 66 \u00b1 6, 83 \u00b1 3 respectively for M(W140\u2013Q299)-(Y866\u2013P1004)-LEH6. Conclusions: We selected two recombinant proteins consisting of two (M(W140\u2013Q299)-(Y866\u2013P1004)-LEH6) or three (M(W140\u2013H328)-(W412\u2013D604)-(Y866\u2013P1004)-LEH6) linked fragments of IgA1 protease A28\u2013P1004 as candidate active component for an antimeningococcal vaccine.<\/jats:p>","DOI":"10.3390\/vaccines12121355","type":"journal-article","created":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T06:58:58Z","timestamp":1733122738000},"page":"1355","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Protective Antimicrobial Effect of the Potential Vaccine Created on the Basis of the Structure of the IgA1 Protease from Neisseria meningitidis"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2603-5506","authenticated-orcid":false,"given":"Yuri","family":"Prokopenko","sequence":"first","affiliation":[{"name":"Laboratory of Antibiotic Resistance, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexei","family":"Zinchenko","sequence":"additional","affiliation":[{"name":"Laboratory of Proteolytic Enzyme Chemistry, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7100-6432","authenticated-orcid":false,"given":"David","family":"Karlinsky","sequence":"additional","affiliation":[{"name":"Laboratory of Proteolytic Enzyme Chemistry, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Kotelnikova","sequence":"additional","affiliation":[{"name":"Laboratory of Proteolytic Enzyme Chemistry, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Razgulyaeva","sequence":"additional","affiliation":[{"name":"Laboratory \u201cPolymers for Biology\u201d, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Gordeeva","sequence":"additional","affiliation":[{"name":"Laboratory of Proteolytic Enzyme Chemistry, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Nokel","sequence":"additional","affiliation":[{"name":"Laboratory of Proteolytic Enzyme Chemistry, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4941-8913","authenticated-orcid":false,"given":"Oxana","family":"Serova","sequence":"additional","affiliation":[{"name":"Laboratory of Proteolytic Enzyme Chemistry, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Kaliberda","sequence":"additional","affiliation":[{"name":"Laboratory of Proteolytic Enzyme Chemistry, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larisa","family":"Zhigis","sequence":"additional","affiliation":[{"name":"Laboratory \u201cPolymers for Biology\u201d, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lev","family":"Rumsh","sequence":"additional","affiliation":[{"name":"Laboratory of Proteolytic Enzyme Chemistry, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0384-6568","authenticated-orcid":false,"given":"Ivan","family":"Smirnov","sequence":"additional","affiliation":[{"name":"Laboratory of Proteolytic Enzyme Chemistry, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia"},{"name":"Laboratory of Protein Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia"},{"name":"Laboratory of Biotechnology of Recombinant Hormonal Drugs, Endocrinology Research Centre, 117292 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Pizza, M., Bekkat-Berkani, R., and Rappuoli, R. 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