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Galicia","award":["PID2022-139720OB-I00"],"award-info":[{"award-number":["PID2022-139720OB-I00"]}]},{"name":"Centro de investigaci\u00f3n do Sistema universitario de Galicia","award":["ED431C 2021\/29"],"award-info":[{"award-number":["ED431C 2021\/29"]}]},{"name":"Centro de investigaci\u00f3n do Sistema universitario de Galicia","award":["ED431G 2023\/03"],"award-info":[{"award-number":["ED431G 2023\/03"]}]},{"name":"European Union","award":["E-RTA2014-00006-C02-00"],"award-info":[{"award-number":["E-RTA2014-00006-C02-00"]}]},{"name":"European Union","award":["2024ICT283"],"award-info":[{"award-number":["2024ICT283"]}]},{"name":"European Union","award":["PID2022-139720OB-I00"],"award-info":[{"award-number":["PID2022-139720OB-I00"]}]},{"name":"European Union","award":["ED431C 2021\/29"],"award-info":[{"award-number":["ED431C 2021\/29"]}]},{"name":"European Union","award":["ED431G 2023\/03"],"award-info":[{"award-number":["ED431G 2023\/03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Background\/Objectives: Crimean\u2013Congo hemorrhagic fever virus (CCHFV) is an emerging, widely distributed zoonotic tick-borne pathogen. The virus causes severe disease in humans, and numerous wild and domestic animals act as reservoirs of it. Unfortunately, there are no effective therapies or safe vaccines commercialized nowadays for this particular virus. As CCHF (Crimean\u2013Congo hemorrhagic fever) is a serious threat to public health, there is an urgent need to investigate the development of safe and effective vaccination strategies further. Methods: In this work, we have employed two immunization platforms based on protein nanoparticles and a modified vaccinia Ankara (MVA) viral vector using the nucleoprotein (NP) as the target antigen. The humoral and cellular immune responses were characterized by ELISA, ICS, and cytokine measurement. Results: This work shows that a single dose of the vaccine candidates was not as immunogenic as the heterologous vaccination using nanoparticles and MVA. A prime with NP nanoparticles (NS-NP) and a boost with MVA-expressing NP were capable of triggering significant levels of humoral and cellular immune responses against CCHFV in mice. Conclusions: Our study shows that the NS-NP\/MVA-NP vaccination strategy effectively elicits a robust humoral and cellular immune response in a mouse model, emphasizing its potential as a protective approach against CCHFV lineages.<\/jats:p>","DOI":"10.3390\/vaccines13030291","type":"journal-article","created":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T06:59:59Z","timestamp":1741589999000},"page":"291","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Prime-Boost Vaccination Based on Nanospheres and MVA Encoding the Nucleoprotein of Crimean-Congo Hemorrhagic Fever Virus Elicits Broad Immune Responses"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4667-4081","authenticated-orcid":false,"given":"Eva","family":"Calvo-Pinilla","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal, INIA, CSIC, Valdeolmos, 28130 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3548-037X","authenticated-orcid":false,"given":"Sandra","family":"Moreno","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal, INIA, CSIC, Valdeolmos, 28130 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8822-7709","authenticated-orcid":false,"given":"Natalia","family":"Barreiro-Pi\u00f1eiro","sequence":"additional","affiliation":[{"name":"Centro Singular de Investigaci\u00f3n en Qu\u00edmica Biol\u00f3xica e Materiais Moleculares (CIQUS), Departamento de Bioqu\u00edmica e Biolox\u00eda Molecular, Universidade de Santiago de Compostela, 15705 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3966-3499","authenticated-orcid":false,"given":"Juana M.","family":"S\u00e1nchez-Puig","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda, Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA-CSIC), Ctra. de la Coru\u00f1a km 7.5, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8819-5767","authenticated-orcid":false,"given":"Rafael","family":"Blasco","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda, Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA-CSIC), Ctra. de la Coru\u00f1a km 7.5, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8877-7775","authenticated-orcid":false,"given":"Jos\u00e9","family":"Mart\u00ednez-Costas","sequence":"additional","affiliation":[{"name":"Centro Singular de Investigaci\u00f3n en Qu\u00edmica Biol\u00f3xica e Materiais Moleculares (CIQUS), Departamento de Bioqu\u00edmica e Biolox\u00eda Molecular, Universidade de Santiago de Compostela, 15705 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7865-538X","authenticated-orcid":false,"given":"Alejandro","family":"Brun","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal, INIA, CSIC, Valdeolmos, 28130 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1869-9051","authenticated-orcid":false,"given":"Gema","family":"Lorenzo","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal, INIA, CSIC, Valdeolmos, 28130 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"120","DOI":"10.4269\/ajtmh.1987.36.120","article-title":"Epidemiologic and Clinical Features of Crimean-Congo Hemorrhagic Fever in Southern Africa","volume":"36","author":"Swanepoel","year":"1987","journal-title":"Am. 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