{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:00:10Z","timestamp":1760058010203,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of National Science and Technology of China","award":["2012ZX10002001-003-003","2012ZX10004-904","2018ZX10715005"],"award-info":[{"award-number":["2012ZX10002001-003-003","2012ZX10004-904","2018ZX10715005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Background: Various factors influence the immunologic responses to HBV vaccines in adults, including unchangeable individual characteristics. Personalized vaccination regimens accounting for host factors can enhance immune efficiency, particularly for adults at higher risk. Methods: In this two-center controlled trial, HBV vaccine-na\u00efve participants aged 25\u201355 were randomly administered the two types of HBV vaccines (yeast cell-derived (YDV) or Chinese hamster ovary (CHO) cell-derived) at 0\u20131\u20136 months. Antibody titers were measured eight weeks after the final dose. Results: Overall, 289 participants with YDV and 293 participants with CHO completed the three-dose series and antibody testing. The seroprotection rates (SPRs) were comparable (97.23% vs. 98.98%; p = 0.1398), but the geometric mean concentration (GMC) was significantly higher for the CHO (1627.83 mIU\/mL vs. 600.76 mIU\/mL; p &lt; 0.0001). The GMC of both regimens declined significantly in individuals aged \u226545 years and males. Unlike the YDV, the GMC of CHO was minimally affected by BMI or smoking or drinking status. Conclusion: The CHO regimen may be advantageous for HBV vaccine-na\u00efve adults aged 25\u201355 with BMI \u2265 25 or those who smoke or drink, in terms of immunogenicity and durability, providing insights for personalized immunization strategies.<\/jats:p>","DOI":"10.3390\/vaccines13030295","type":"journal-article","created":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T12:02:59Z","timestamp":1741608179000},"page":"295","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of Yeast and CHO Cell-Derived Hepatitis B Vaccines and Influencing Factors in Vaccine-Na\u00efve Adults in China: Insights for Personalized Immunization Strategies"],"prefix":"10.3390","volume":"13","author":[{"given":"Qian","family":"Qiu","sequence":"first","affiliation":[{"name":"Beijing Center for Disease Prevention and Control, Beijing 100013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huai","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Center for Disease Prevention and Control, Beijing 100013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Center for Disease Prevention and Control, Beijing 100013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,10]]},"reference":[{"key":"ref_1","unstructured":"WHO (2024, December 25). Hepatitis B. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/hepatitis-b."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Mahmood, F., Xu, R., Awan, M.U.N., Song, Y., Han, Q., Xia, X., Wei, J., Xu, J., Peng, J., and Zhang, J. (2023). HBV Vaccines: Advances and Development. Vaccines, 11.","DOI":"10.3390\/vaccines11121862"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e00084-18","DOI":"10.1128\/CMR.00084-18","article-title":"Factors That Influence the Immune Response to Vaccination","volume":"32","author":"Zimmermann","year":"2019","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Manfr\u00e3o-Netto, J.H.C., Gomes, A.M.V., and Parachin, N.S. (2019). Advances in Using Hansenula polymorpha as Chassis for Recombinant Protein Production. Front. Bioeng. Biotechnol., 7.","DOI":"10.3389\/fbioe.2019.00094"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/S0264-410X(96)00239-3","article-title":"Comparison between hepatitis B surface antigen (HBsAg) particles derived from mammalian cells (CHO) and yeast cells (Hansenula polymorpha): Composition, structure and immunogenicity","volume":"15","author":"Diminsky","year":"1997","journal-title":"Vaccine"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.jchromb.2006.03.064","article-title":"Molecular characterization of recombinant Hepatitis B surface antigen from Chinese hamster ovary and Hansenula polymorpha cells by high-performance size exclusion chromatography and multi-angle laser light scattering","volume":"838","author":"Zhou","year":"2006","journal-title":"J. Chromatogr. B Analyt. Technol. Biomed. Life Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1016\/S1473-3099(20)30780-5","article-title":"Immunogenicity and safety of a tri-antigenic versus a mono-antigenic hepatitis B vaccine in adults (PROTECT): A randomised, double-blind, phase 3 trial","volume":"21","author":"Vesikari","year":"2021","journal-title":"Lancet Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Yang, S., Tian, G., Cui, Y., Ding, C., Deng, M., Yu, C., Xu, K., Ren, J., Yao, J., and Li, Y. (2016). Factors influencing immunologic response to hepatitis B vaccine in adults. Sci. Rep., 6.","DOI":"10.1038\/srep27251"},{"key":"ref_9","unstructured":"WHO (2024). Obesity and Overweight, WHO. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/obesity-and-overweight."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1086\/314578","article-title":"What level of hepatitis B antibody is protective?","volume":"179","author":"Jack","year":"1999","journal-title":"J. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1080\/21645515.2018.1438090","article-title":"Comparison of immunogenicity between hepatitis B vaccines with different dosages and schedules among healthy young adults in China: A 2-year follow-up study","volume":"14","author":"Wang","year":"2018","journal-title":"Hum. Vacc. Immunother."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1080\/21645515.2018.1509172","article-title":"Suitable hepatitis B vaccine for adult immunization in China: A systematic review and meta-analysis","volume":"15","author":"Wu","year":"2019","journal-title":"Hum. Vacc. Immunother."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1056\/NEJMoa1007644","article-title":"Nucleic Acid Testing to Detect HBV Infection in Blood Donors","volume":"364","author":"Stramer","year":"2011","journal-title":"N. Engl. J. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1016\/S0140-6736(22)01468-4","article-title":"Hepatitis B","volume":"401","author":"Jeng","year":"2023","journal-title":"Lancet"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1002\/hep.32474","article-title":"Protection and antibody levels 35 years after primary series with hepatitis B vaccine and response to a booster dose","volume":"76","author":"Bruce","year":"2022","journal-title":"Hepatology"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Cocchio, S., Baldo, V., Volpin, A., Fonzo, M., Floreani, A., Furlan, P., Mason, P., Trevisan, A., and Scapellato, M.L. (2021). Persistence of Anti-Hbs after up to 30 Years in Health Care Workers Vaccinated against Hepatitis B Virus. Vaccines, 9.","DOI":"10.3390\/vaccines9040323"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12979-020-00179-9","article-title":"The SENIEUR protocol and the efficacy of hepatitis B vaccination in healthy elderly persons by age, gender, and vaccine route","volume":"17","author":"Edelman","year":"2020","journal-title":"Immun. Ageing"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1038\/s41577-019-0180-1","article-title":"Mechanisms underlying T cell ageing","volume":"19","author":"Goronzy","year":"2019","journal-title":"Nat. Rev. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Huang, Y.T., Zheng, H.J., Zhu, Y.W., Hong, Y., Zha, J., Lin, Z.J., Li, Z.F., Wang, C.Y., Fang, Z.H., and Yu, X.X. (2023). Loss of CD28 expression associates with severe T-cell exhaustion in acute myeloid leukemia. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1139517"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Trevisan, A., Giuliani, A., Scapellato, M.L., Anticoli, S., Carsetti, R., Zaffina, S., Brugaletta, R., Vonesch, N., Tomao, P., and Ruggieri, A. (2020). Sex Disparity in Response to Hepatitis B Vaccine Related to the Age of Vaccination. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17010327"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Fathi, A., Addo, M.M., and Dahlke, C. (2021). Sex Differences in Immunity: Implications for the Development of Novel Vaccines Against Emerging Pathogens. Front. Immunol., 11.","DOI":"10.3389\/fimmu.2020.601170"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/S0091-6749(99)70503-8","article-title":"Estrogen enhances immunoglobulin production by human PBMCs","volume":"103","author":"Kanda","year":"1999","journal-title":"J. Allergy Clin. Immun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1046\/j.1365-2249.1996.d01-842.x","article-title":"Testosterone inhibits immunoglobulin production by human peripheral blood mononuclear cells","volume":"106","author":"Kanda","year":"1996","journal-title":"Clin. Exp. Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1093\/trstmh\/tru167","article-title":"Sex-based differences in immune function and responses to vaccination","volume":"109","author":"Klein","year":"2015","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_25","first-page":"103","article-title":"Contribution of sex-based immunological differences to the enhanced immune response in female mice following vaccination with hepatitis B vaccine","volume":"20","author":"Li","year":"2019","journal-title":"Mol. Med. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"416","DOI":"10.3345\/kjp.2019.00563","article-title":"Association between Body Mass Index and Hepatitis B antibody seropositivity in children","volume":"62","author":"Kwon","year":"2019","journal-title":"Korean J. Pediatr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.ijid.2020.01.047","article-title":"Immune response to different types of hepatitis B vaccine booster doses 2\u201332 years after the primary immunization schedule and its influencing factors","volume":"93","author":"Zhao","year":"2020","journal-title":"Int. J. Infect. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/s41541-020-00266-4","article-title":"Reduced immune responses to hepatitis B primary vaccination in obese individuals with nonalcoholic fatty liver disease (NAFLD)","volume":"6","author":"Joshi","year":"2021","journal-title":"npj Vaccines"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3603","DOI":"10.1016\/j.vaccine.2013.05.003","article-title":"Young and elderly patients with type 2 diabetes have optimal B cell responses to the seasonal influenza vaccine","volume":"31","author":"Frasca","year":"2013","journal-title":"Vaccine"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1080\/21645515.2016.1274475","article-title":"Influences of obesity on the immunogenicity of Hepatitis B vaccine","volume":"13","author":"Liu","year":"2017","journal-title":"Hum. Vacc. Immunother."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Young, K.M., Gray, C.M., and Bekker, L.G. (2013). Is Obesity a Risk Factor for Vaccine Non-Responsiveness?. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0082779"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4835","DOI":"10.1016\/j.vaccine.2016.08.027","article-title":"Hepatitis B vaccine response in obesity: A meta-analysis","volume":"34","author":"Fan","year":"2016","journal-title":"Vaccine"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"ckad160-1425","DOI":"10.1093\/eurpub\/ckad160.1425","article-title":"Effect of smoking on long-term immunity after hepatitis B vaccine in infancy. A 20-year cohort study","volume":"33","author":"Fonzo","year":"2023","journal-title":"Eur. J. Public Health"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"268","DOI":"10.18632\/oncotarget.13613","article-title":"Impacts of cigarette smoking on immune responsiveness: Up and down or upside down?","volume":"8","author":"Qiu","year":"2017","journal-title":"Oncotarget"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Morojele, N.K., Shenoi, S.V., Shuper, P.A., Braithwaite, R.S., and Rehm, J. (2021). Alcohol Use and the Risk of Communicable Diseases. Nutrients, 13.","DOI":"10.3390\/nu13103317"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ruiz-Cortes, K., Villageliu, D.N., and Samuelson, D.R. (2022). Innate lymphocytes: Role in alcohol-induced immune dysfunction. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.934617"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"10052","DOI":"10.12998\/wjcc.v9.i33.10052","article-title":"Effects of alcohol consumption on viral hepatitis B and C","volume":"9","author":"Xu","year":"2021","journal-title":"World J. Clin. Cases"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Rastogi, I., Jeon, D., Moseman, J.E., Muralidhar, A., Potluri, H.K., and McNeel, D.G. (2022). Role of B cells as antigen presenting cells. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.954936"}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/13\/3\/295\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:50:15Z","timestamp":1760028615000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/13\/3\/295"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,10]]},"references-count":38,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["vaccines13030295"],"URL":"https:\/\/doi.org\/10.3390\/vaccines13030295","relation":{},"ISSN":["2076-393X"],"issn-type":[{"type":"electronic","value":"2076-393X"}],"subject":[],"published":{"date-parts":[[2025,3,10]]}}}