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In this study, we investigated whether combining RSV and VZV vaccines could reduce the number of vaccination injections, thereby minimizing discomfort for elderly individuals and reducing manufacturing costs. Methods: In this study, we developed two types of combined RSV and VZV mRNA vaccines. Using RSV and VZV mRNA vaccines administered alone as controls, we evaluated the immune response elicited by the combined mRNA vaccines in C57BL\/6J mice. Results: The results demonstrated that RSV mRNA, VZV mRNA, and a mixture of both could be effectively encapsulated in lipid nanoparticles (LNPs) with uniform particle sizes. Compared to the administration of either the RSV or VZV mRNA vaccine alone, the delivery of two kinds of mRNA LNP combination formulation\u2014whether directly mixed or encapsulated two mRNAs in the same LNP formulation\u2014elicited comparable IgG titers, neutralization titers, cell-mediated immunity (CMI), and CD4+ T-cell responses. Conclusions: In conclusion, this study establishes the feasibility of combining RSV and VZV mRNA-LNP vaccines, laying a solid foundation for clinical trials of combined RSV and VZV vaccines.<\/jats:p>","DOI":"10.3390\/vaccines13040361","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T06:06:35Z","timestamp":1743141995000},"page":"361","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Immunogenicity Evaluation of Combination Respiratory Syncytial Virus and Varicella\u2013Zoster Virus mRNA Vaccines in C57BL\/6J Mice"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8559-7464","authenticated-orcid":false,"given":"Ning","family":"Luan","sequence":"first","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luxia","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingping","family":"Hu","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haihao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Gao","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhentao","family":"Lei","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Cao","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cunbao","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e70016","DOI":"10.1002\/mco2.70016","article-title":"Vaccine and therapeutic agents against the respiratory syncytial virus: Resolved and unresolved issue","volume":"5","author":"Li","year":"2024","journal-title":"MedComm"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.medmal.2018.01.008","article-title":"Respiratory syncytial virus infection in elderly adults","volume":"48","author":"Haber","year":"2018","journal-title":"Med. Mal. Infect."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1858","DOI":"10.21037\/tp-24-271","article-title":"Prevention of respiratory syncytial virus from 1991 to 2024: A systematic review and bibliometrics analysis","volume":"13","author":"Li","year":"2024","journal-title":"Transl. Pediatr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1007\/s40121-023-00802-4","article-title":"Incidence of Respiratory Syncytial Virus Infection in Older Adults: Limitations of Current Data","volume":"12","author":"Rozenbaum","year":"2023","journal-title":"Infect. Dis. Ther."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"15016","DOI":"10.1038\/nrdp.2015.16","article-title":"Varicella zoster virus infection","volume":"1","author":"Gershon","year":"2015","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"6776","DOI":"10.1016\/j.vaccine.2019.09.031","article-title":"VZV-specific cell-mediated immunity, but not humoral immunity, correlates inversely with the incidence of herpes zoster and the severity of skin symptoms and zoster-associated pain: The SHEZ study","volume":"37","author":"Asada","year":"2019","journal-title":"Vaccine"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1056\/NEJMoa2035389","article-title":"Efficacy and Safety of the mRNA-1273 SARS-CoV-2 Vaccine","volume":"384","author":"Baden","year":"2021","journal-title":"N. Engl. J. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2603","DOI":"10.1056\/NEJMoa2034577","article-title":"Safety and Efficacy of the BNT162b2 mRNA COVID-19 Vaccine","volume":"383","author":"Polack","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1093\/infdis\/jit365","article-title":"Safety and immunogenicity of an AS01-adjuvanted varicella-zoster virus subunit candidate vaccine against herpes zoster in adults \u226550 years of age","volume":"208","author":"Chlibek","year":"2013","journal-title":"J. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1056\/NEJMoa1603800","article-title":"Efficacy of the Herpes Zoster Subunit Vaccine in Adults 70 Years of Age or Older","volume":"375","author":"Cunningham","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/j.ymthe.2019.01.020","article-title":"mRNA as a Transformative Technology for Vaccine Development to Control Infectious Diseases","volume":"27","author":"Maruggi","year":"2019","journal-title":"Mol. Ther."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5793","DOI":"10.1016\/j.vaccine.2020.06.062","article-title":"Immunogenicity generated by mRNA vaccine encoding VZV gE antigen is comparable to adjuvanted subunit vaccine and better than live attenuated vaccine in nonhuman primates","volume":"38","author":"Monslow","year":"2020","journal-title":"Vaccine"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2309985","DOI":"10.1080\/22221751.2024.2309985","article-title":"Herpes zoster mRNA vaccine induces superior vaccine immunity over licensed vaccine in mice and rhesus macaques","volume":"13","author":"Huang","year":"2024","journal-title":"Emerg. Microbes Infect."},{"key":"ref_14","unstructured":"(2023, May 04). FDA Approves First Respiratory Syncytial Virus (Rsv) Vaccine, Available online: https:\/\/www.fda.gov\/news-events\/press-announcements\/fda-approves-first-respiratory-syncytial-virus-rsv-vaccine."},{"key":"ref_15","unstructured":"(2023, June 02). US FDA Approves ABRYSVO, Pfizer\u2019s Vaccine for the Prevention of Respiratory Syncytial Virus (RSV) in Older Adults. Available online: https:\/\/www.pfizer.com\/news\/press-release\/press-release-detail\/us-fda-approves-abrysvotm-pfizers-vaccine-prevention."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"eade0424","DOI":"10.1126\/scitranslmed.ade0424","article-title":"A prefusion-stabilized RSV F subunit vaccine elicits B cell responses with greater breadth and potency than a postfusion F vaccine","volume":"14","author":"Chang","year":"2022","journal-title":"Sci. Transl. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.anai.2020.03.017","article-title":"The journey to a respiratory syncytial virus vaccine","volume":"125","author":"Mejias","year":"2020","journal-title":"Ann. Allergy Asthma Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"11377","DOI":"10.1128\/JVI.74.23.11377-11387.2000","article-title":"Glycoprotein E of varicella-zoster virus enhances cell-cell contact in polarized epithelial cells","volume":"74","author":"Mo","year":"2000","journal-title":"J. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Cao, H., Wang, Y., Luan, N., Lin, K., and Liu, C. (2021). Effects of Varicella-Zoster Virus Glycoprotein E Carboxyl-Terminal Mutation on mRNA Vaccine Efficacy. Vaccines, 9.","DOI":"10.3390\/vaccines9121440"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"12406","DOI":"10.1128\/JVI.78.22.12406-12415.2004","article-title":"Functions of the C-terminal domain of varicella-zoster virus glycoprotein E in viral replication in vitro and skin and T-cell tropism in vivo","volume":"78","author":"Moffat","year":"2004","journal-title":"J. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Wang, Y., Cao, H., Lin, K., Hu, J., Luan, N., and Liu, C. (2023). Evaluation of the Immunological Efficacy of an LNP-mRNA Vaccine Prepared from Varicella Zoster Virus Glycoprotein gE with a Double-Mutated Carboxyl Terminus in Different Untranslated Regions in Mice. Vaccines, 11.","DOI":"10.3390\/vaccines11091475"},{"key":"ref_22","unstructured":"(2022). Shaw Christine, and August Allison. (Patent WO 2022\/221336 A1), Respiratory Syncytial Virus mRNA Vaccines."},{"key":"ref_23","unstructured":"World Health Organization (2021, June 07). Messenger RNA Encoding the Full-Length SARS-CoV-2 Spike Glycoprotein. Available online: https:\/\/web.archive.org\/web\/20210105162941\/https:\/\/mednet-communities.net\/inn\/db\/media\/docs\/11889.doc."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.virs.2022.06.002","article-title":"LNP-CpG ODN-adjuvanted varicella-zoster virus glycoprotein E induced comparable levels of immunity with Shingrix in VZV-primed mice","volume":"37","author":"Luan","year":"2022","journal-title":"Virol. Sin."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Xu, S., Yang, K., Li, R., and Zhang, L. (2020). mRNA Vaccine Era-Mechanisms, Drug Platform and Clinical Prospection. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21186582"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Liu, B., Cao, B., Wang, C., Han, B., Sun, T., Miao, Y., Lu, Q., and Cui, F. (2022). Immunogenicity and Safety of Childhood Combination Vaccines: A Systematic Review and Meta-Analysis. Vaccines, 10.","DOI":"10.3390\/vaccines10030472"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1971","DOI":"10.1038\/s41564-023-01505-9","article-title":"Immune imprinting and next-generation coronavirus vaccines","volume":"8","author":"Huang","year":"2023","journal-title":"Nat. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2494","DOI":"10.1080\/21645515.2017.1362515","article-title":"Effect of combination vaccines on completion and compliance of childhood vaccinations in the United States","volume":"13","author":"Kurosky","year":"2017","journal-title":"Hum. Vaccin. Immunother."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1128\/CMR.00090-16","article-title":"The Human Immune Response to Respiratory Syncytial Virus Infection","volume":"30","author":"Russell","year":"2017","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"301","DOI":"10.3109\/08916939409009532","article-title":"Human Th1 and Th2 cells: Functional properties, regulation of development and role in autoimmunity","volume":"18","author":"Zancuoghi","year":"1994","journal-title":"Autoimmunity"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1016\/j.vaccine.2014.01.019","article-title":"Safety and immunogenicity of three different formulations of an adjuvanted varicella-zoster virus subunit candidate vaccine in older adults: A phase II, randomized, controlled study","volume":"32","author":"Chlibek","year":"2014","journal-title":"Vaccine"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1056\/NEJMoa2213836","article-title":"Efficacy and Safety of a Bivalent RSV Prefusion F Vaccine in Older Adults","volume":"388","author":"Walsh","year":"2023","journal-title":"N. Engl. J. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1732","DOI":"10.1093\/cid\/ciae010","article-title":"Efficacy and Safety of Respiratory Syncytial Virus (RSV) Prefusion F Protein Vaccine (RSVPreF3 OA) in Older Adults Over 2 RSV Seasons","volume":"78","author":"Ison","year":"2024","journal-title":"Clin. Infect. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Xia, X. (2021). Detailed Dissection and Critical Evaluation of the Pfizer\/BioNTech and Moderna mRNA Vaccines. Vaccines, 9.","DOI":"10.3390\/vaccines9070734"}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/13\/4\/361\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:04:09Z","timestamp":1760029449000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/13\/4\/361"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,28]]},"references-count":34,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["vaccines13040361"],"URL":"https:\/\/doi.org\/10.3390\/vaccines13040361","relation":{},"ISSN":["2076-393X"],"issn-type":[{"value":"2076-393X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,28]]}}}