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Among these are inactivated virus vaccines like CoronaVac (Sinovac Biotech), which are used in several countries to reduce the pandemic\u2019s effects. However, its use was associated with low protection, particularly against novel virus variants that quickly appeared in the following months. Vaccines play a crucial role in activating the immune system to combat infections, with Memory B-cells being a key part of this mechanism, eliciting protective neutralizing antibodies. This work focused on studying B-cell memory repertoire after two consecutive doses of CoronaVac. Methodology: Memory B-cells were isolated from five CoronaVac vaccinated and five pre-pandemic individuals and subsequently stimulated in vitro before high-throughput Illumina sequencing of the Heavy Chain Variable repertoire. Results: We observed a shift in the VH repertoire with increased HCDR3 length and enrichment of IGVH 3-23, 3-30, 3-7, 3-72, and 3-74 for IgA BCRs and IGHV 4-39 and 4-59 for IgG BCRs. A high expansion of IgA-specific clonal populations was observed in vaccinated individuals relative to pre-pandemic controls, accompanied by shared IgA variable heavy chain (VH) sequences among memory B cells across different vaccine recipients of IgA clones was also observed in vaccinated individuals compared to pre-pandemic controls, with several IgA VH sharing between memory B cells from different vaccines. Moreover, a high convergence was observed among vaccinees and SARS-CoV-2 neutralizing antibody sequences found in the CoV-abDab database. Conclusion: These data show the ability of CoronaVac to elicit antibodies with characteristics similar to those previously identified as neutralizing antibodies, supporting its protective efficacy. Furthermore, this analysis of the immunological repertoire in the context of viral infections reinforces the importance of immunization in generating convergent antibodies for the antiviral response.<\/jats:p>","DOI":"10.3390\/vaccines13040393","type":"journal-article","created":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T05:03:07Z","timestamp":1744174987000},"page":"393","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Naive and Memory B Cell BCR Repertoires in Individuals Immunized with an Inactivated SARS-CoV-2 Vaccine"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4930-0971","authenticated-orcid":false,"given":"Renato Kaylan Alves de Oliveira","family":"Fran\u00e7a","sequence":"first","affiliation":[{"name":"Department of Cellular Biology, Institute of Biological Science, University of Bras\u00edlia, Brasilia 70910-900, DF, Brazil"},{"name":"Molecular Pathology Post-Graduation Program, University of Bras\u00edlia, Brasilia 70910-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6834-5756","authenticated-orcid":false,"given":"Pedro Henrique Arag\u00e3o","family":"Barros","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology, Institute of Biological Science, University of Bras\u00edlia, Brasilia 70910-900, DF, Brazil"},{"name":"Molecular Biology Post-Graduation Program, University of Bras\u00edlia, Brasilia 70910-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacyelle Medeiros","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology, Institute of Biological Science, University of Bras\u00edlia, Brasilia 70910-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4531-5781","authenticated-orcid":false,"given":"Hitallo Guilherme Costa","family":"Fontinele","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology, Institute of Biological Science, University of Bras\u00edlia, Brasilia 70910-900, DF, Brazil"},{"name":"Molecular Pathology Post-Graduation Program, University of Bras\u00edlia, Brasilia 70910-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1946-7191","authenticated-orcid":false,"given":"Andrea Queiroz","family":"Maranh\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology, Institute of Biological Science, University of Bras\u00edlia, Brasilia 70910-900, DF, Brazil"},{"name":"Molecular Pathology Post-Graduation Program, University of Bras\u00edlia, Brasilia 70910-900, DF, Brazil"},{"name":"Molecular Biology Post-Graduation Program, University of Bras\u00edlia, Brasilia 70910-900, DF, Brazil"},{"name":"III-Immunology Investigation Institute, National Institute of Science and Technology (iii-INCT), Brasilia 70067-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7136-7059","authenticated-orcid":false,"given":"Marcelo de Macedo","family":"Brigido","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology, Institute of Biological Science, University of Bras\u00edlia, Brasilia 70910-900, DF, Brazil"},{"name":"Molecular Pathology Post-Graduation Program, University of Bras\u00edlia, Brasilia 70910-900, DF, Brazil"},{"name":"Molecular Biology Post-Graduation Program, University of Bras\u00edlia, Brasilia 70910-900, DF, Brazil"},{"name":"III-Immunology Investigation Institute, National Institute of Science and Technology (iii-INCT), Brasilia 70067-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Focosi, D., Franchini, M., Maggi, F., and Shoham, S. (2024). COVID-19 therapeutics. Clin. Microbiol. Rev., 37.","DOI":"10.1128\/cmr.00119-23"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Georgieva, E., Ananiev, J., Yovchev, Y., Arabadzhiev, G., Abrashev, H., Abrasheva, D., Atanasov, V., Kostandieva, R., Mitev, M., and Petkova-Parlapanska, K. (2023). COVID-19 Complications: Oxidative Stress, Inflammation, and Mitochondrial and Endothelial Dysfunction. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241914876"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1038\/s41591-022-01701-w","article-title":"Vaccine effectiveness of heterologous CoronaVac plus BNT162b2 in Brazil","volume":"28","author":"Katikireddi","year":"2022","journal-title":"Nat. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e527","DOI":"10.1016\/S2666-5247(21)00129-4","article-title":"Neutralisation of SARS-CoV-2 lineage P.1 by antibodies elicited through natural SARS-CoV-2 infection or vaccination with an inactivated SARS-CoV-2 vaccine: An immunological study","volume":"2","author":"Souza","year":"2021","journal-title":"Lancet Microbe"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1038\/s41591-022-01704-7","article-title":"Neutralizing antibodies against the SARS-CoV-2 Omicron variant BA.1 following homologous and heterologous CoronaVac or BNT162b2 vaccination","volume":"28","author":"Cheng","year":"2022","journal-title":"Nat. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"245","DOI":"10.3934\/microbiol.2023015","article-title":"SARS-CoV-2 infection and immune responses","volume":"9","author":"Harne","year":"2023","journal-title":"AIMS Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Galson, J.D., Schaetzle, S., Bashford-Rogers, R.J.M., Raybould, M.I.J., Kovaltsuk, A., Kilpatrick, G.J., Minter, R., Finch, D.K., Dias, J., and James, L.K. (2020). Deep Sequencing of B Cell Receptor Repertoires From COVID-19 Patients Reveals Strong Convergent Immune Signatures. Front. Immunol., 11.","DOI":"10.3389\/fimmu.2020.605170"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e0160021","DOI":"10.1128\/jvi.01600-21","article-title":"Analysis of B Cell Receptor Repertoires Reveals Key Signatures of the Systemic B Cell Response after SARS-CoV-2 Infection","volume":"96","author":"Zhang","year":"2022","journal-title":"J. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"eabd2223","DOI":"10.1126\/scitranslmed.abd2223","article-title":"IgA dominates the early neutralizing antibody response to SARS-CoV-2","volume":"13","author":"Sterlin","year":"2021","journal-title":"Sci. Transl. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1016\/j.cell.2020.08.046","article-title":"Longitudinal Isolation of Potent Near-Germline SARS-CoV-2-Neutralizing Antibodies from COVID-19 Patients","volume":"182","author":"Kreer","year":"2020","journal-title":"Cell"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1080\/22221751.2021.1925594","article-title":"Germline IGHV3-53-encoded RBD-targeting neutralizing antibodies are commonly present in the antibody repertoires of COVID-19 patients","volume":"10","author":"Yan","year":"2021","journal-title":"Emerg. Microbes Infect."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e13309","DOI":"10.1111\/irv.13309","article-title":"Cross-Reactive Antibody Responses to Coronaviruses Elicited by SARS-CoV-2 Infection or Vaccination","volume":"18","author":"Lee","year":"2024","journal-title":"Influenza Other Respir. Viruses"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/j.1365-2249.2010.04206.x","article-title":"Reference values for B cell subpopulations from infancy to adulthood","volume":"162","author":"Morbach","year":"2010","journal-title":"Clin. Exp. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1002\/eji.200839129","article-title":"Clonal dissection of the human memory B-cell repertoire following infection and vaccination","volume":"39","author":"Pinna","year":"2009","journal-title":"Eur. J. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Waltari, E., McGeever, A., Friedland, N., Kim, P.S., and McCutcheon, K.M. (2019). Functional Enrichment and Analysis of Antigen-Specific Memory B Cell Antibody Repertoires in PBMCs. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.01452"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Petrova, V.N., Muir, L., McKay, P.F., Vassiliou, G.S., Smith, K.G.C., Lyons, P.A., Russell, C.A., Anderson, C.A., Kellam, P., and Bashford-Rogers, R.J.M. (2018). Combined Influence of B-Cell Receptor Rearrangement and Somatic Hypermutation on B-Cell Class-Switch Fate in Health and in Chronic Lymphocytic Leukemia. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.01784"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.jim.2013.02.009","article-title":"Optimization of a human IgG B-cell ELISpot assay for the analysis of vaccine-induced B-cell responses","volume":"391","author":"Jahnmatz","year":"2013","journal-title":"J. Immunol. Methods"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"106944","DOI":"10.1016\/j.imlet.2024.106944","article-title":"Rescuing pathogen-specific memory B-cell from PBMC of prior Zika virus-infected individuals","volume":"271","author":"Silva","year":"2025","journal-title":"Immunol. Lett."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"110393","DOI":"10.1016\/j.celrep.2022.110393","article-title":"B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination","volume":"38","author":"Kotagiri","year":"2022","journal-title":"Cell Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"112780","DOI":"10.1016\/j.celrep.2023.112780","article-title":"Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine","volume":"42","author":"Hoehn","year":"2023","journal-title":"Cell Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1038\/s41435-024-00270-x","article-title":"Comparative single-cell transcriptomic profile of hybrid immunity induced by adenovirus vector-based COVID-19 vaccines","volume":"25","author":"Wan","year":"2024","journal-title":"Genes Immun."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.jim.2008.02.010","article-title":"Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning","volume":"334","author":"Tiller","year":"2008","journal-title":"J. Immunol. Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1946","DOI":"10.1172\/JCI44447","article-title":"The majority of intestinal IgA+ and IgG+ plasmablasts in the human gut are antigen-specific","volume":"121","author":"Benckert","year":"2011","journal-title":"J. Clin. Investig."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1866","DOI":"10.1136\/annrheumdis-2015-208356","article-title":"Single cell cloning and recombinant monoclonal antibodies generation from RA synovial B cells reveal frequent targeting of citrullinated histones of NETs","volume":"75","author":"Corsiero","year":"2016","journal-title":"Ann. Rheum. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2698","DOI":"10.1002\/eji.201545526","article-title":"Direct high-throughput amplification and sequencing of immunoglobulin genes from single human B cells","volume":"45","author":"Murugan","year":"2015","journal-title":"Eur. J. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3047","DOI":"10.1093\/bioinformatics\/btw354","article-title":"MultiQC: Summarize analysis results for multiple tools and samples in a single report","volume":"32","author":"Ewels","year":"2016","journal-title":"Bioinformatics"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3356","DOI":"10.1093\/bioinformatics\/btv359","article-title":"Change-O: A toolkit for analyzing large-scale B cell immunoglobulin repertoire sequencing data","volume":"31","author":"Gupta","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1930","DOI":"10.1093\/bioinformatics\/btu138","article-title":"pRESTO: A toolkit for processing high-throughput sequencing raw reads of lymphocyte receptor repertoires","volume":"30","author":"Heiden","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Gabernet, G., Marquez, S., Bjornson, R., Peltzer, A., Meng, H., Aron, E., Lee, N.Y., Jensen, C.G., Ladd, D., and Polster, M. (2024). nf-core\/airrflow: An adaptive immune receptor repertoire analysis workflow employing the Immcantation framework. PLoS Comput. Biol., 20.","DOI":"10.1101\/2024.01.18.576147"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"W34","DOI":"10.1093\/nar\/gkt382","article-title":"IgBLAST: An immunoglobulin variable domain sequence analysis tool","volume":"41","author":"Ye","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_32","unstructured":"Csardi, G., and Nepusz, T. (2025, March 30). The Igraph Software Package for Complex Network Research, InterJournal, Complex Systems 1695. Available online: http:\/\/igraph.org."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1038\/s41375-023-01952-7","article-title":"Clonotype definitions for immunogenetic studies: Proposals from the EuroClonality NGS Working Group","volume":"37","author":"Sofou","year":"2023","journal-title":"Leukemia"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1093\/bioinformatics\/btaa739","article-title":"CoV-AbDab: The coronavirus antibody database","volume":"37","author":"Raybould","year":"2021","journal-title":"Bioinformatics"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1038\/s41586-022-04980-y","article-title":"BA.2.12.1, BA.4 and BA.5 escape antibodies elicited by Omicron infection","volume":"608","author":"Cao","year":"2022","journal-title":"Nature"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/s41421-021-00292-z","article-title":"Three epitope-distinct human antibodies from RenMab mice neutralize SARS-CoV-2 and cooperatively minimize the escape of mutants","volume":"7","author":"Nie","year":"2021","journal-title":"Cell Discov."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Han, X., Wang, Y., Li, S., Hu, C., Li, T., Gu, C., Wang, K., Shen, M., Wang, J., and Hu, J. (2021). A Rapid and Efficient Screening System for Neutralizing Antibodies and Its Application for SARS-CoV-2. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.653189"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e20220826","DOI":"10.1084\/jem.20220826","article-title":"Humoral immunity to SARS-CoV-2 elicited by combination COVID-19 vaccination regimens","volume":"219","author":"Wang","year":"2022","journal-title":"J. Exp. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"109822","DOI":"10.1016\/j.celrep.2021.109822","article-title":"Landscape of human antibody recognition of the SARS-CoV-2 receptor binding domain","volume":"37","author":"Wheatley","year":"2021","journal-title":"Cell Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"eabf1555","DOI":"10.1126\/scitranslmed.abf1555","article-title":"Enhanced SARS-CoV-2 neutralization by dimeric IgA","volume":"13","author":"Wang","year":"2021","journal-title":"Sci. Transl. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1038\/s41586-021-04060-7","article-title":"Anti-SARS-CoV-2 receptor-binding domain antibody evolution after mRNA vaccination","volume":"600","author":"Cho","year":"2021","journal-title":"Nature"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1080\/07853890.2022.2031272","article-title":"Comparison of the deep immune profiling of B cell subsets between healthy adults and Sj\u00f6gren\u2019s syndrome","volume":"54","author":"Feng","year":"2022","journal-title":"Ann. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"103659","DOI":"10.1016\/j.isci.2021.103659","article-title":"Persistence of functional memory B cells recognizing SARS-CoV-2 variants despite loss of specific IgG","volume":"25","author":"Winklmeier","year":"2022","journal-title":"iScience"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"97","DOI":"10.12688\/wellcomeopenres.11386.1","article-title":"Optimisation of ex vivo memory B cell expansion\/differentiation for interrogation of rare peripheral memory B cell subset responses","volume":"2","author":"Muir","year":"2017","journal-title":"Wellcome Open Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"4500","DOI":"10.1182\/blood-2002-11-3569","article-title":"A role for Toll-like receptors in acquired immunity: Up-regulation of TLR9 by BCR triggering in naive B cells and constitutive expression in memory B cells","volume":"101","author":"Bernasconi","year":"2003","journal-title":"Blood"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Marsman, C., Verhoeven, D., Koers, J., Rispens, T., Brinke, A.T., van Ham, S.M., and Kuijpers, T.W. (2022). Optimized Protocols for In-Vitro T-Cell-Dependent and T-Cell-Independent Activation for B-Cell Differentiation Studies Using Limited Cells. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.815449"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"126563","DOI":"10.1016\/j.vaccine.2024.126563","article-title":"Assessing human B cell responses to influenza virus vaccines and adjuvants in a PBMC-derived in vitro culture system","volume":"44","author":"Gong","year":"2025","journal-title":"Vaccine"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1038\/s41421-021-00250-9","article-title":"Immune memory in convalescent patients with asymptomatic or mild COVID-19","volume":"7","author":"Long","year":"2021","journal-title":"Cell Discov."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1016\/j.immuni.2016.10.027","article-title":"Identification of a CD4-Binding-Site Antibody to HIV that Evolved Near-Pan Neutralization Breadth","volume":"45","author":"Huang","year":"2016","journal-title":"Immunity"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e01090","DOI":"10.1002\/cti2.1090","article-title":"Distinguishing naive- from memory-derived human B cells during acute responses","volume":"8","author":"Auladell","year":"2019","journal-title":"Clin. Transl. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Marti, Z., Ruder, J., Thomas, O.G., Bronge, M., Soto, L.D.L.P., Gr\u00f6nlund, H., Olsson, T., and Martin, R. (2024). Enhanced and cross-reactive in vitro memory B cell response against Epstein-Barr virus nuclear antigen 1 in multiple sclerosis. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1334720"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1002\/jmr.679","article-title":"Length of the antibody heavy chain complementarity determining region 3 as a specificity-determining factor","volume":"17","author":"Barrios","year":"2004","journal-title":"J. Mol. Recognit."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Jin, X., Jin, X., Zhou, W., Zhou, W., Luo, M., Luo, M., Wang, P., Wang, P., Xu, Z., and Xu, Z. (2021). Global characterization of B cell receptor repertoire in COVID-19 patients by single-cell V(D)J sequencing. Brief. Bioinform., 22.","DOI":"10.1093\/bib\/bbab192"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Liu, H., Kaku, C.I., Song, G., Yuan, M., Andrabi, R., Burton, D.R., Walker, L.M., and Wilson, I.A. (2022). Human antibodies to SARS-CoV-2 with a recurring YYDRxG motif retain binding and neutralization to variants of concern including Omicron. Commun. Biol., 5.","DOI":"10.1038\/s42003-022-03700-6"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2734","DOI":"10.1038\/s41467-024-46982-6","article-title":"The SARS-CoV-2 neutralizing antibody response to SD1 and its evasion by BA.2.86","volume":"15","author":"Zhou","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/j.immuni.2022.03.019","article-title":"A large-scale systematic survey reveals recurring molecular features of public antibody responses to SARS-CoV-2","volume":"55","author":"Wang","year":"2022","journal-title":"Immunity"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1038\/s41467-023-37972-1","article-title":"Vaccination of SARS-CoV-2-infected individuals expands a broad range of clonally diverse affinity-matured B cell lineages","volume":"14","author":"Chernyshev","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"114307","DOI":"10.1016\/j.celrep.2024.114307","article-title":"Deep repertoire mining uncovers ultra-broad coronavirus neutralizing antibodies targeting multiple spike epitopes","volume":"43","author":"Hurtado","year":"2024","journal-title":"Cell Rep."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e159062","DOI":"10.1172\/JCI159062","article-title":"An antibody targeting the N-terminal domain of SARS-CoV-2 disrupts the spike trimer","volume":"132","author":"Suryadevara","year":"2022","journal-title":"J. Clin. Investig."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Quinti, I., Mortari, E.P., Salinas, A.F., Milito, C., and Carsetti, R. (2021). IgA Antibodies and IgA Deficiency in SARS-CoV-2 Infection. Front. Cell Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.655896"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e20220638","DOI":"10.1084\/jem.20220638","article-title":"Potent human broadly SARS-CoV-2-neutralizing IgA and IgG antibodies effective against Omicron BA.1 and BA.2","volume":"219","author":"Planchais","year":"2022","journal-title":"J. Exp. Med."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.chom.2020.09.002","article-title":"Human B Cell Clonal Expansion and Convergent Antibody Responses to SARS-CoV-2","volume":"28","author":"Nielsen","year":"2020","journal-title":"Cell Host Microbe"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1038\/s41586-020-2456-9","article-title":"Convergent antibody responses to SARS-CoV-2 in convalescent individuals","volume":"584","author":"Robbiani","year":"2020","journal-title":"Nature"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2096970","DOI":"10.1080\/21645515.2022.2096970","article-title":"CoronaVac: A review of efficacy, safety, and immunogenicity of the inactivated vaccine against SARS-CoV-2","volume":"18","author":"Jin","year":"2022","journal-title":"Hum. Vaccin. Immunother."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"M\u00e9ndez, C., Pe\u00f1aloza, H.F., Schultz, B.M., Pi\u00f1a-Iturbe, A., R\u00edos, M., Moreno-Tapia, D., Pereira-S\u00e1nchez, P., Leighton, D., Orellana, C., and Covarrubias, C. (2023). Humoral and cellular response induced by a second booster of an inactivated SARS-CoV-2 vaccine in adults. EBioMedicine, 91.","DOI":"10.1016\/j.ebiom.2023.104563"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1186\/s12931-023-02542-y","article-title":"Effectiveness of the booster dose of inactivated COVID-19 vaccine against Omicron BA.5 infection: A matched cohort study of adult close contacts","volume":"24","author":"Zeng","year":"2023","journal-title":"Respir. Res."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1038\/s41586-022-04466-x","article-title":"Memory B cell repertoire from triple vaccinees against diverse SARS-CoV-2 variants","volume":"603","author":"Wang","year":"2022","journal-title":"Nature"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1007\/s13238-020-00807-6","article-title":"Single cell RNA and immune repertoire profiling of COVID-19 patients reveal novel neutralizing antibody","volume":"12","author":"Li","year":"2021","journal-title":"Protein Cell"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2007","DOI":"10.1080\/22221751.2022.2105261","article-title":"Comparative global B cell receptor repertoire difference induced by SARS-CoV-2 infection or vaccination via single-cell V(D)J sequencing","volume":"11","author":"He","year":"2022","journal-title":"Emerg. Microbes Infect."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Fraley, E.R., Khanal, S., Pierce, S.H., LeMaster, C.A., McLennan, R., Pastinen, T., and Bradley, T. (2022). Effects of Prior Infection with SARS-CoV-2 on B Cell Receptor Repertoire Response during Vaccination. 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