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Allergen-specific immunotherapy (AIT) has emerged as a disease-modifying therapy for FA. However, due to long-term treatment duration and unexpected adverse reactions, only a minority of patients benefit from AIT. Therefore, effective prophylactic interventions are urgently needed for FA patients. Methods: In this proof-of-concept study, using a well-established mRNA platform, we developed mRNA vaccine candidates encoding for the major egg white allergen Gal d2 and comprehensively evaluated their prophylactic efficacy against anaphylaxis in a Gal d2-induced allergic mouse model. Results: Two vaccine formulations, Gal d2 mRNA vaccine and Gal d2-IL-10 mRNA vaccine, both demonstrated potent ability in inducing allergen-specific IgG and Th1-type T cells. Importantly, the two vaccine formulations showed promise in preventing the onset of allergic disease, which is indicated by prevention of body temperature decline during anaphylaxis. Conclusions: We provided preliminary proof-of-concept evidence showing that the mRNA platform is unique and holds promise for the development of anti-allergy vaccines. This is largely attributed to the capacities of mRNA vaccines in eliciting an allergen-blocking antibody, shifting Th2 towards Th1 immunity, as well as in generating peripheral tolerance. However, further investigations are required to better understand the mode of action.<\/jats:p>","DOI":"10.3390\/vaccines13050448","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T06:18:02Z","timestamp":1745475482000},"page":"448","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Leveraging an mRNA Platform for the Development of Vaccines Against Egg Allergy"],"prefix":"10.3390","volume":"13","author":[{"given":"Xianyu","family":"Shao","sequence":"first","affiliation":[{"name":"Center for Infectious Medicine and Vaccine Research, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China"},{"name":"Institute of Translational Medicine, China Pharmaceutical University, Nanjing 211198, China"},{"name":"Innovation Center for Nucleic Acid Medicine, Institute for Innovative Drug Development and Life Sciences, Wuxi 214000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijing","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Infectious Medicine and Vaccine Research, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China"},{"name":"Institute of Translational Medicine, China Pharmaceutical University, Nanjing 211198, China"},{"name":"Innovation Center for Nucleic Acid Medicine, Institute for Innovative Drug Development and Life Sciences, Wuxi 214000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changzhen","family":"Weng","sequence":"additional","affiliation":[{"name":"Center for Infectious Medicine and Vaccine Research, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China"},{"name":"Institute of Translational Medicine, China Pharmaceutical University, Nanjing 211198, 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