{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T23:38:10Z","timestamp":1787614690828,"version":"build-2736575974"},"reference-count":50,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Investigator-Initiated Studies Program of Merck Sharp &amp; Dohme doo","award":["101128"],"award-info":[{"award-number":["101128"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Background\/Objectives: Measles, mumps, and rubella (MMR) continue to pose a significant public health challenge due to insufficient immunization coverage. This study aimed to provide the first seroprevalence data against MMR and to explore self-reported immunity among future healthcare workers (HCWs) in Serbia, including women of childbearing age. Methods: We included 1296 future health care workers (HCWs) aged 19 to 29, born in Serbia. All HCWs supplied a blood sample for serology and filled in a questionnaire. Antibodies were measured using an enzyme immunoassay against measles, mumps, and rubella (MMR). Results: Anti-measles, -mumps, and -rubella seronegativity rates were 25.6%, 26.5%, and 4.4%, respectively, among future HCWs in Serbia. The mumps seronegativity rate was significantly higher in the oldest (27\u201329-year) age group, accompanied by significantly lower anti-mumps IgG GMCs in the same age group compared to younger participants (p = 0.035 and p &lt; 0.001, respectively). Anti-mumps seronegativity also increased significantly across birth cohorts, from the youngest to the oldest (p = 0.004). Furthermore, anti-mumps IgG antibody GMCs were significantly higher among females, those who attended nursery\/kindergarten, and unvaccinated individuals (p = 0.050, p = 0.020, and p = 0.005, respectively). Finally, older age and unvaccinated status were identified as independent factors associated with anti-measles and anti-mumps seronegativity among future HCWs in Serbia. Conclusions: The cross-sectional seroprevalence data revealed insufficient seroprotection in this population of particular importance, i.e., future HCWs, and women of childbearing age. These results strongly support the national recommendations for the mandatory vaccination of these populations. Identified immunity gaps should be closed promptly by strategic, targeted serologic screening, followed by vaccination of those lacking MMR antibodies.<\/jats:p>","DOI":"10.3390\/vaccines13070700","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T07:42:06Z","timestamp":1751355726000},"page":"700","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Seroprevalence of Measles-, Mumps-, and Rubella-Specific Antibodies in Future Healthcare Workers in Serbia: A Cross-Sectional Study"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6829-096X","authenticated-orcid":false,"given":"Ana","family":"Banko","sequence":"first","affiliation":[{"name":"Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7542-5690","authenticated-orcid":false,"given":"Andja","family":"Cirkovic","sequence":"additional","affiliation":[{"name":"Institute for Medical Statistics and Informatics, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2052-6232","authenticated-orcid":false,"given":"Vladimir","family":"Petrovic","sequence":"additional","affiliation":[{"name":"Institute of Public Health of Vojvodina, 21000 Novi Sad, Serbia"},{"name":"Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6923-189X","authenticated-orcid":false,"given":"Mioljub","family":"Ristic","sequence":"additional","affiliation":[{"name":"Institute of Public Health of Vojvodina, 21000 Novi Sad, Serbia"},{"name":"Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9561-7825","authenticated-orcid":false,"given":"Vladimir","family":"Vukovic","sequence":"additional","affiliation":[{"name":"Institute of Public Health of Vojvodina, 21000 Novi Sad, Serbia"},{"name":"Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dobrila","family":"Stankovic-Djordjevic","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Medicine, University of Nis, 18000 Nis, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9446-8701","authenticated-orcid":false,"given":"Danijela","family":"Miljanovic","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,27]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2024). Meeting of the Strategic Advisory Group of Experts on Immunization, March 2024: Conclusions and Recommendations. Wkly. Epidemiol. Rec., 99, 285\u2013306."},{"key":"ref_2","unstructured":"World Health Organization (2012). Global Measles and Rubella Strategic Plan: 2012\u20132020, World Health Organization."},{"key":"ref_3","unstructured":"European Centre for Disease Prevention and Control (ECDC) (2025, May 13). Measles on the Rise Again in Europe: Time to Check Your Vaccination Status. Available online: https:\/\/www.ecdc.europa.eu\/en\/news-events\/measles-rise-again-europe-time-check-your-vaccination-status."},{"key":"ref_4","unstructured":"Institute of Public Health \u201cDr Milan Jovanovic Batut\u201d (2023). Report on Communicable Diseases in the Republic of Serbia for the Year 2022, Institute of Public Health \u201cDr Milan Jovanovic Batut\u201d."},{"key":"ref_5","unstructured":"(2024, December 28). IZJZS-Batut. Available online: https:\/\/www.batut.org.rs\/index.php?content=2776."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Pati\u0107, A., \u0160trbac, M., Petrovi\u0107, V., Milo\u0161evi\u0107, V., Risti\u0107, M., Cvjetkovi\u0107, I.H., and Medi\u0107, S. (2020). Seroepidemiological Study of Rubella in Vojvodina, Serbia: 24 Years after the Introduction of the MMR Vaccine in the National Immunization Programme. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0227413"},{"key":"ref_7","unstructured":"Institute of Public Health of Serbia \u201cDr Milan Jovanovi\u0107 Batut\u201d (2023). Report on the Implementation of Immunization in the Territory of the Republic of Serbia in 2022, Institute of Public Health \u201cDr Milan Jovanovic Batut\u201d."},{"key":"ref_8","unstructured":"UNICEF (2018). Knowledge, Attitudes and Practices in Relation to Immunization of Children In Serbia, UNICEF Belgrade."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2000417","DOI":"10.2807\/1560-7917.ES.2021.26.26.2000417","article-title":"Trend in Mandatory Immunisation Coverage: Linear and Joinpoint Regression Approach, Serbia, 2000 to 2017","volume":"26","author":"Veljkovic","year":"2021","journal-title":"Eurosurveillance"},{"key":"ref_10","unstructured":"Institute of Public Health of Serbia \u201cDr Milan Jovanovic Batut\u201d (2024). The Current Epidemiological Situation of Measles (Morbilli) in the Republic of Serbia, Institute of Public Health \u201cDr Milan Jovanovic Batut\u201d."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Risti\u0107, M., Milo\u0161evi\u0107, V., Medi\u0107, S., Malba\u0161a, J.D., Raj\u010devi\u0107, S., Boban, J., and Petrovi\u0107, V. (2019). Sero-Epidemiological Study in Prediction of the Risk Groups for Measles Outbreaks in Vojvodina, Serbia. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0216219"},{"key":"ref_12","unstructured":"Institute of Public Helath of Serbia \u201cDr Milan Jovanovic Batut\u201d (2025). Mandatory Immunization Schedule in the Republic of Serbia, Institut of Public Health \u201cDr Milan Jovanovic Batut\u201d."},{"key":"ref_13","unstructured":"(2025, May 14). Official Gazette of the Republic of Serbia Rulebook on Immunization and Chemoprophylaxis: 11\/2006-114, 25\/2013-36, 63\/2013-264, 99\/2013-18, 118\/2013-130, 65\/2014-38, 32\/2015-105. Available online: https:\/\/pravno-informacioni-sistem.rs\/SlGlasnikPortal\/reg\/viewAct\/7648144d-9226-46bc-90f5-16fe5dc2201d."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"20592","DOI":"10.1074\/jbc.RA117.000147","article-title":"Data Visualization, Bar Naked: A Free Tool for Creating Interactive Graphics","volume":"292","author":"Weissgerber","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2400684","DOI":"10.2807\/1560-7917.ES.2025.30.16.2400684","article-title":"Seroprevalence against Measles, Austria, Stratified by Birth Years 1922 to 2024","volume":"30","author":"Springer","year":"2025","journal-title":"Eurosurveillance"},{"key":"ref_16","first-page":"18-00224","article-title":"Measles Outbreak in a Tertiary Level Hospital, Porto, Portugal, 2018: Challenges in the Post-Elimination Era","volume":"23","author":"Almeida","year":"2018","journal-title":"Eurosurveillance"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1016\/j.vaccine.2019.02.068","article-title":"Hospital Outbreak of Measles\u2014Evaluation and Costs of 10 Occupational Cases among Healthcare Worker in Germany, February to March 2017","volume":"37","author":"Hiller","year":"2019","journal-title":"Vaccine"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.ajic.2018.09.003","article-title":"Mumps Outbreak with High Complication Rates among Residents in a University Teaching Hospital","volume":"47","year":"2019","journal-title":"Am. J. Infect. Control."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1900114","DOI":"10.2807\/1560-7917.ES.2019.24.17.1900114","article-title":"Measles Outbreak in Gothenburg Urban Area, Sweden, 2017 to 2018: Low Viral Load in Breakthrough Infections","volume":"24","author":"Sundell","year":"2019","journal-title":"Eurosurveillance"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1016\/j.vaccine.2017.02.018","article-title":"Immunity against Vaccine-Preventable Diseases in Finnish Pediatric Healthcare Workers in 2015","volume":"35","author":"Koivisto","year":"2017","journal-title":"Vaccine"},{"key":"ref_21","first-page":"2001167","article-title":"Immunity to Vaccine-Preventable Diseases among Paediatric Healthcare Workers in Denmark, 2019","volume":"26","author":"Kirkby","year":"2019","journal-title":"Eurosurveillance"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1017\/S0950268813002859","article-title":"Healthcare Workers and Measles-Mumps-Rubella (MMR) Status: How Worried Should We Be about Further Outbreaks?","volume":"142","author":"Basu","year":"2014","journal-title":"Epidemiol. Infect."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1053\/jhin.2002.1222","article-title":"Susceptibility of Healthcare Workers to Measles, Mumps Rubella and Varicella","volume":"51","author":"Fedeli","year":"2002","journal-title":"J. Hosp. Infect."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/S1473-3099(20)30442-4","article-title":"Immunogenicity and Persistence of Trivalent Measles, Mumps, and Rubella Vaccines: A Systematic Review and Meta-Analysis","volume":"21","author":"Schenk","year":"2021","journal-title":"Lancet Infect. Dis."},{"key":"ref_25","first-page":"100128","article-title":"Seroprevalence of Measles-, Mumps-, and Rubella-Specific Antibodies in the German Adult Population\u2014Cross-Sectional Analysis of the German Health Interview and Examination Survey for Adults (DEGS1)","volume":"7","author":"Friedrich","year":"2021","journal-title":"Lancet Reg. Health-Eur."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Perfetto, B., Paduano, G., Grimaldi, E., Sansone, V., Donnarumma, G., and Di Giuseppe, G. (2024). Seroprevalence for Measles, Varicella, Mumps and Rubella in the Trainee Obstetric Population: A Survey in Southern Italy. Vaccines, 12.","DOI":"10.3390\/vaccines12030335"},{"key":"ref_27","first-page":"CD004407","article-title":"Vaccines for Measles, Mumps, Rubella, and Varicella in Children","volume":"11","author":"Rivetti","year":"2021","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1093\/infdis\/jis568","article-title":"Waning Antibody Levels and Avidity: Implications for MMR Vaccine-Induced Protection","volume":"206","author":"Kontio","year":"2012","journal-title":"J. Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1080\/21645515.2020.1871296","article-title":"Long-Term Immunogenicity after Measles Vaccine vs. Wild Infection: An Italian Retrospective Cohort Study","volume":"17","author":"Bianchi","year":"2021","journal-title":"Hum. Vaccines Immunother."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1213\/ANE.0000000000001196","article-title":"Resurgence of Vaccine-Preventable Diseases in the United States: Anesthetic and Critical Care Implications","volume":"122","author":"Porteous","year":"2016","journal-title":"Anesth. Analg."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2522","DOI":"10.1080\/21645515.2021.1876486","article-title":"Seroprevalence against the Measles Virus after Vaccination or Natural Infection in an Adult Population in Madinah, Saudi Arabia","volume":"17","author":"Mahallawi","year":"2021","journal-title":"Hum. Vaccines Immunother."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1093\/infdis\/jiw480","article-title":"Measles Outbreak among Previously Immunized Healthcare Workers, The Netherlands, 2014","volume":"214","author":"Lochlainn","year":"2016","journal-title":"J. Infect. Dis."},{"key":"ref_33","unstructured":"(2024, December 28). Vaccine Scheduler|ECDC Vaccine Scheduler. Available online: https:\/\/vaccine-schedule.ecdc.europa.eu\/Scheduler\/ByDisease?SelectedDiseaseId=3&SelectedCountryIdByDisease=-1."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"7949","DOI":"10.1016\/j.vaccine.2011.08.081","article-title":"Sero-Epidemiology of Measles-Specific IgG Antibodies and Predictive Factors for Low or Missing Titres in a German Population-Based Cross-Sectional Study in Children and Adolescents (KiGGS)","volume":"29","author":"Mankertz","year":"2011","journal-title":"Vaccine"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1700642","DOI":"10.2807\/1560-7917.ES.2018.23.38.1700642","article-title":"Large Outbreak of Mumps Virus Genotype g among Vaccinated Students in Norway, 2015 to 2016","volume":"23","author":"Veneti","year":"2018","journal-title":"Eurosurveillance"},{"key":"ref_36","first-page":"46958019894098","article-title":"Measles Outbreak in Unvaccinated and Partially Vaccinated Children and Adults in the United States and Canada (2018\u20132019): A Narrative Review of Cases","volume":"56","author":"Sanyaolu","year":"2019","journal-title":"Inq. J. Health Care Organ. Provis. Financ."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e194515","DOI":"10.1001\/jamapediatrics.2019.4515","article-title":"Clinical Practices for Measles-Mumps-Rubella Vaccination among US Pediatric International Travelers","volume":"174","author":"Hyle","year":"2020","journal-title":"JAMA Pediatr."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Medi\u0107, S., Petrovi\u0107, V., Lon\u00e8arevi\u0107, G., Kanazir, M., Lazarevi\u0107, I.B., Adrovi\u0107, S.R., Ban\u00e8evi\u0107, M., Muller, C.P., and H\u00fcbschen, J.M. (2019). Epidemiological, Clinical and Laboratory Characteristics of the Measles Resurgence in the Republic of Serbia in 2014-2015. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0224009"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e766","DOI":"10.1016\/S2468-2667(24)00181-6","article-title":"Long-Term Waning of Vaccine-Induced Immunity to Measles in England: A Mathematical Modelling Study","volume":"9","author":"Robert","year":"2024","journal-title":"Lancet Public Health"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2490","DOI":"10.1016\/S0140-6736(17)31463-0","article-title":"Measles","volume":"390","author":"Moss","year":"2017","journal-title":"Lancet"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e001417","DOI":"10.1136\/bmjph-2024-001417","article-title":"Seroprevalence of Measles Antibodies in Women Born between 1985 and 1999 in Metropolitan City in India: A Cross-Sectional Study","volume":"2","author":"Hadaye","year":"2024","journal-title":"BMJ Public Health"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1093\/infdis\/jit143","article-title":"Waning of Maternal Antibodies against Measles, Mumps, Rubella, and Varicella in Communities with Contrasting Vaccination Coverage","volume":"208","author":"Waaijenborg","year":"2013","journal-title":"J. Infect. Dis."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1097\/GRF.0b013e31824df256","article-title":"Measles, Mumps, and Rubella","volume":"55","author":"White","year":"2012","journal-title":"Clin. Obstet. Gynecol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1086\/313928","article-title":"Preparing for Elimination of Congenital Rubella Syndrome (CRS): Summary of a Workshop on CRS Elimination in the United States","volume":"31","author":"Reef","year":"2000","journal-title":"Clin. Infect. Dis."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.ijid.2020.09.1477","article-title":"Prevalence of Herpes-, Measles Morbillivirus-, Parvovirus B19- and Rubella Viruses Immunoglobulin G among Women with Chronic Hepatitis B of Reproductive Age in Denmark: A Cross-Sectional Study","volume":"101","author":"Hallager","year":"2020","journal-title":"Int. J. Infect. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2851","DOI":"10.1080\/21645515.2019.1621146","article-title":"Measles in Pregnancy: A Threat for Italian Women?","volume":"15","author":"Marchi","year":"2019","journal-title":"Hum. Vaccines Immunother."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1093\/oxfordjournals.epirev.a036121","article-title":"Herd Immunity: History, Theory, Practice","volume":"15","author":"Fine","year":"1993","journal-title":"Epidemiol. Rev."},{"key":"ref_48","unstructured":"(2025, May 13). Official Gazette of the Republic of Serbia Rulebook on the Program of Mandatory and Recommended Immunization of the Population Against Certain Infectious Diseases No 88\/2018, 11\/2018, 14\/2018, 45\/2018, 48\/2018, 58\/2018, 104\/2018, 6\/2021, 52\/2021 i 66\/2022. Available online: https:\/\/www.paragraf.rs\/propisi\/pravilnik-o-programu-obavezne-i-preporucene-imunizacije-stanovnistva-protiv-odredjenih-zaraznih-bolesti.html."},{"key":"ref_49","unstructured":"(2025, May 13). Official Gazette of the Republic of Serbia Rulebook on the Program of Mandatory and Recommended Immunization of the Population Against Certain Communicable Diseases No 23\/2023. Available online: https:\/\/www.paragraf.rs\/propisi\/pravilnik-o-programu-obavezne-i-preporucene-imunizacije-stanovnistva-protiv-odredjenih-zaraznih-bolesti.html."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.jiph.2011.11.003","article-title":"Prevaccination Screening of Health-Care Workers for Immunity to Measles, Rubella, Mumps, and Varicella in a Developing Country: What Do We Save?","volume":"5","author":"Alp","year":"2012","journal-title":"J. Infect. Public Health"}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/13\/7\/700\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:00:36Z","timestamp":1760032836000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/13\/7\/700"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,27]]},"references-count":50,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["vaccines13070700"],"URL":"https:\/\/doi.org\/10.3390\/vaccines13070700","relation":{},"ISSN":["2076-393X"],"issn-type":[{"value":"2076-393X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,27]]}}}