{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:38:51Z","timestamp":1760060331333,"version":"build-2065373602"},"reference-count":17,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Beijing Minhai Biotechnology Co., Ltd."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Background\/Objectives: This study aimed to develop a new attenuated live mumps vaccine strain and determine its biological properties and effectiveness. Methods: Plaque purification and amplification were performed in chicken embryo cells. Candidate live attenuated mumps MuV-365 strain sequencing was performed. After evaluating the potential neurotoxicity of the MuV-365 mumps strain, a preclinical safety evaluation of measles\u2013mumps\u2013rubella (MMR) live attenuated vaccine containing the MuV-365 strain was performed to support the registration and application of the MMR vaccine. Finally, mumps neutralization antibody titers and the concentration of anti-serum mumps-specific IgG were determined to evaluate the immunogenicity and efficacy of the MuV-365 strain and MMR vaccine in mice and rhesus monkeys. Results: The plaque of the PL-KUM main seed virus was screened, and strains whose sequences were highly homologous to RIT4385 (JL-5 derived) were selected to amplify. The candidate live attenuated mumps MuV-365 strain was then developed. Safety evaluation results indicated that the MuV-365 strain had no potential neurotoxicity, and the MMR vaccine containing the MuV-365 strain also showed no significant safety hazard. The immunogenicity of MuV-365 strain in BALB\/c mice was not inferior to S79 and PL-KUM. After two doses of the MuV-365 strain, the concentration of anti-serum mumps-specific IgG of the MuV-365 strain was significantly higher than that of the S79 strain (p &lt; 0.01). In rhesus monkeys, the MMR vaccine had good immunogenicity against measles and rubella after one dose, while immunogenicity against mumps improved after two doses. Conclusions: The developed MuV-365 strain was genetically stable, with adequate safety and immunogenicity.<\/jats:p>","DOI":"10.3390\/vaccines13080879","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T08:40:48Z","timestamp":1755765648000},"page":"879","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and Biological Properties of a New Live Attenuated Mumps Vaccine Strain"],"prefix":"10.3390","volume":"13","author":[{"given":"Xue","family":"Song","sequence":"first","affiliation":[{"name":"Beijing Minhai Biotechnology Co., Ltd., Beijing 102609, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiumei","family":"Ren","sequence":"additional","affiliation":[{"name":"Beijing Minhai Biotechnology Co., Ltd., Beijing 102609, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Song","sequence":"additional","affiliation":[{"name":"Beijing Minhai Biotechnology Co., Ltd., Beijing 102609, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengbao","family":"Yang","sequence":"additional","affiliation":[{"name":"Beijing Minhai Biotechnology Co., Ltd., Beijing 102609, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kailang","family":"Lu","sequence":"additional","affiliation":[{"name":"Beijing Minhai Biotechnology Co., Ltd., Beijing 102609, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Minhai Biotechnology Co., Ltd., Beijing 102609, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiankai","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Minhai Biotechnology Co., Ltd., Beijing 102609, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Su, S.B., Chang, H.L., and Chen, A.K. 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Vaccine Immunol."}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/13\/8\/879\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:31:40Z","timestamp":1760034700000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/13\/8\/879"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,20]]},"references-count":17,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["vaccines13080879"],"URL":"https:\/\/doi.org\/10.3390\/vaccines13080879","relation":{},"ISSN":["2076-393X"],"issn-type":[{"type":"electronic","value":"2076-393X"}],"subject":[],"published":{"date-parts":[[2025,8,20]]}}}