{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T04:25:00Z","timestamp":1769747100866,"version":"3.49.0"},"reference-count":27,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yunnan Province Major Science and Technology Special Project","award":["202302AA310003"],"award-info":[{"award-number":["202302AA310003"]}]},{"name":"Yunnan Province Major Science and Technology Special Project","award":["202402AA310022"],"award-info":[{"award-number":["202402AA310022"]}]},{"name":"Yunnan Province Major Science and Technology Special Project","award":["202002AA100009"],"award-info":[{"award-number":["202002AA100009"]}]},{"name":"Yunnan Province Major Science and Technology Special Project","award":["2021-I2M-1-043"],"award-info":[{"award-number":["2021-I2M-1-043"]}]},{"name":"Yunnan Province Major Science and Technology Special Project","award":["202401AT070147"],"award-info":[{"award-number":["202401AT070147"]}]},{"name":"CAMS Innovation Fund for Medical Sciences","award":["202302AA310003"],"award-info":[{"award-number":["202302AA310003"]}]},{"name":"CAMS Innovation Fund for Medical Sciences","award":["202402AA310022"],"award-info":[{"award-number":["202402AA310022"]}]},{"name":"CAMS Innovation Fund for Medical Sciences","award":["202002AA100009"],"award-info":[{"award-number":["202002AA100009"]}]},{"name":"CAMS Innovation Fund for Medical Sciences","award":["2021-I2M-1-043"],"award-info":[{"award-number":["2021-I2M-1-043"]}]},{"name":"CAMS Innovation Fund for Medical Sciences","award":["202401AT070147"],"award-info":[{"award-number":["202401AT070147"]}]},{"name":"Special Project in Basic Research of Yunnan Province","award":["202302AA310003"],"award-info":[{"award-number":["202302AA310003"]}]},{"name":"Special Project in Basic Research of Yunnan Province","award":["202402AA310022"],"award-info":[{"award-number":["202402AA310022"]}]},{"name":"Special Project in Basic Research of Yunnan Province","award":["202002AA100009"],"award-info":[{"award-number":["202002AA100009"]}]},{"name":"Special Project in Basic Research of Yunnan Province","award":["2021-I2M-1-043"],"award-info":[{"award-number":["2021-I2M-1-043"]}]},{"name":"Special Project in Basic Research of Yunnan Province","award":["202401AT070147"],"award-info":[{"award-number":["202401AT070147"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Background: Combination vaccines protecting against diphtheria, tetanus, pertussis, poliomyelitis, and Haemophilus influenzae type b reduce injection burden and improve compliance. While widely used globally, no domestically produced pentavalent vaccine is currently licensed in China. Recent updates to China\u2019s immunization schedule\u2014including earlier initiation and an added booster for pertussis\u2014highlight the need for compatible combination vaccines. This study evaluated the immunogenicity and feasibility of a novel DTacP-sIPV\/Hib candidate vaccine in preclinical models. Methods: The vaccine was assessed in NIH mice and Wistar rats. Two Hib dosages were tested in mice alongside a DTacP-wIPV\/Hib vaccine (Pentaxim\u00ae). In rats, two sIPV formulations (Formulations A and B) were administered using different intervals (1-month vs. 2-month) and injection methods (mixed vs. separate). Antibody titers were measured by ELISA and poliovirus neutralization assays. Results: The candidate vaccine elicited robust immune responses in both models. In mice, after three doses, the high-dose Hib group achieved &gt;90% seroconversion for pertactin antigen, whereas the low-dose group reached 100% for all antigens. In rats, antibody responses after three doses were comparable to those induced by Pentaxim\u00ae, with no significant differences between immunization schedules or administration routes. Compared with Formulation A (containing a higher type I sIPV antigen content), Formulation B exhibited reduced type I poliovirus neutralization after the first dose (p &lt; 0.05) and delayed seroconversion, while responses to other antigens remained similar. Conclusion: The candidate DTacP-sIPV\/Hib vaccine showed robust immunogenicity and flexibility across schedules and administration methods. A formulation including DT 12.5 Lf, TT 3.5 Lf, PT 25 \u03bcg, FHA 25 \u03bcg, PRN 8 \u03bcg, PRP 10 \u03bcg, and sIPV I\/II\/III at 30\/32\/45 DU is proposed for further development.<\/jats:p>","DOI":"10.3390\/vaccines13100993","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T08:09:42Z","timestamp":1758614982000},"page":"993","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Preclinical Immunogenicity Evaluation of a DTacP-sIPV\/Hib Combination Vaccine in Rodent Models Under Varying Formulations and Immunization Schedules"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3813-7460","authenticated-orcid":false,"given":"Yixian","family":"Fu","sequence":"first","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China"},{"name":"State Key Laboratory of Respiratory Health and Multimorbidity, Beijing 100005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China"},{"name":"State Key Laboratory of Respiratory Health and Multimorbidity, Beijing 100005, China"},{"name":"School of Life Sciences, Yunnan University, Kunming 650091, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lukui","family":"Cai","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China"},{"name":"State Key Laboratory of Respiratory Health and Multimorbidity, Beijing 100005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Medical 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyan","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China"},{"name":"State Key Laboratory of Respiratory Health and Multimorbidity, Beijing 100005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Gao","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China"},{"name":"State Key Laboratory of Respiratory Health and Multimorbidity, Beijing 100005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China"},{"name":"State Key Laboratory of 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