{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:10:20Z","timestamp":1760159420906,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["AGL2017-83226-R","PID2021-122567OB-I00"],"award-info":[{"award-number":["AGL2017-83226-R","PID2021-122567OB-I00"]}]},{"name":"Next Generation EU\/PRTR","award":["AGL2017-83226-R","PID2021-122567OB-I00"],"award-info":[{"award-number":["AGL2017-83226-R","PID2021-122567OB-I00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Introduction\/Background: Rift Valley fever virus (RVFV) and peste des petits ruminants virus (PPRV) are significant pathogens affecting small ruminants, causing substantial economic losses in the affected regions. The development of effective vaccines against both viruses is crucial for disease control. Recombinant viruses expressing heterologous antigens have shown promise as multivalent vaccine candidates. Unlike conventional PPRV vaccines, our recombinant RVFV-vectored vaccines offer a novel dual-protection strategy against RVF and PPR, combining safety, immunogenicity, and a DIVA strategy. Methods: Recombinant RVFVs (ZH548 strain) were generated to express either the hemagglutinin (H) or fusion (F) proteins from the PPRV strain Nigeria 75\/1. The stability of these recombinant viruses was assessed through consecutive passages in cell culture. Immunogenicity studies were carried out in both mice and sheep to assess the induction of cellular and humoral immune responses capable of providing protection against RVFV and PPRV. These studies included intracellular cytokine staining (ICS), IFN-\u03b3 ELISAs, standard ELISAs for antibody detection, and virus neutralization assays. Results: The recombinant RVFVs expressing PPRV H or F proteins demonstrated stability in cell culture, maintaining high viral titers and consistent transgene expression over four passages. Immunization of mice resulted in the production of serum antibodies capable of neutralizing both RVFV and PPRV in vitro as well as cell-mediated immune responses specific to PPRV and RVFV antigens. In mice vaccinated with a high dose (105 pfu), RVFV neutralizing titers reached \u22651:160 and PPRV neutralizing titers ranged from 1:40 to 1:80 by day 30 post-immunization. In sheep, neutralizing antibody titers against RVFV exceeded 1:160 as early as 2 days post-inoculation, while PPRV-specific neutralization titers reached up to 1:80 by day 21 in responsive individuals. In mice, administration of rZH548\u0394NSs:FPPRV elicited a detectable CD8+ IFN\u03b3+ T-cell response against PPRV, with levels ranging from 1.29% to 1.56% for the low and high doses, respectively. In sheep, rZH548\u0394NSs:FPPRV also induced a robust IFN\u03b3 production against PPRV at 14 and 21 days post-infection (dpi). Conclusions: The successful generation and characterization of recombinant RVFVs expressing PPRV antigens demonstrate the potential of using rationally attenuated RVFV as a vector for multivalent vaccine development. Notably, the strategy proved more effective for the recombinant virus expressing the F protein, as it consistently induced more robust cellular and humoral immune responses. These results suggest that this approach could be a viable strategy for simultaneous immunization against Rift Valley fever and other prevalent ruminant diseases, such as peste des petits ruminants. Even though challenge studies were not performed in target species, the strong immune response observed supports including them in future studies.<\/jats:p>","DOI":"10.3390\/vaccines13101039","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T07:56:29Z","timestamp":1759996589000},"page":"1039","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Single Dose of Live-Attenuated Rift Valley Fever Virus Vector Expressing Peste Des Petits Ruminants Virus (PPRV) H or F Antigens Induces Immunity in Sheep"],"prefix":"10.3390","volume":"13","author":[{"given":"Sandra","family":"Moreno","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal (CISA), Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria, Consejo Superior de Investigaciones Cient\u00edficas (INIA-CSIC), Valdeolmos, 28130 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1869-9051","authenticated-orcid":false,"given":"Gema","family":"Lorenzo","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal (CISA), Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria, Consejo Superior de Investigaciones Cient\u00edficas (INIA-CSIC), Valdeolmos, 28130 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1486-1656","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal (CISA), Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria, Consejo Superior de Investigaciones Cient\u00edficas (INIA-CSIC), Valdeolmos, 28130 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9661-3226","authenticated-orcid":false,"given":"Celia","family":"Alonso","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal (CISA), Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria, Consejo Superior de Investigaciones Cient\u00edficas (INIA-CSIC), Valdeolmos, 28130 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9737-337X","authenticated-orcid":false,"given":"Friedemann","family":"Weber","sequence":"additional","affiliation":[{"name":"Institute for Virology, FB10-Veterinary Medicine, Justus-Liebig University, 35392 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bel\u00e9n","family":"Borrego","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal (CISA), Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria, Consejo Superior de Investigaciones Cient\u00edficas (INIA-CSIC), Valdeolmos, 28130 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Brun","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal (CISA), Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria, Consejo Superior de Investigaciones Cient\u00edficas (INIA-CSIC), Valdeolmos, 28130 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"883","DOI":"10.2460\/javma.234.7.883","article-title":"Rift Valley Fever Virus","volume":"234","author":"Bird","year":"2009","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1007\/s11262-025-02164-0","article-title":"Recent Advances in Treatment and Detection of Rift Valley Fever Virus: A Comprehensive Overview","volume":"61","author":"Yekanipour","year":"2025","journal-title":"Virus Genes"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"002025","DOI":"10.1099\/jgv.0.002025","article-title":"Tissue Distribution and Transmission of Rift Valley Fever Phlebovirus in European Culex Pipiens and Aedes Albopictus Mosquitoes Following Intrathoracic Inoculation","volume":"105","author":"Gardela","year":"2024","journal-title":"J. Gen. Virol."},{"key":"ref_4","first-page":"197","article-title":"Rift Valley Fever Virus (Family Bunyaviridae, Genus Phlebovirus). Isolations from Diptera Collected during an Inter-Epizootic Period in Kenya","volume":"95","author":"Linthicum","year":"1985","journal-title":"Epidemiol. Infect."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1099\/jgv.0.000765","article-title":"Rift Valley Fever Virus: Strategies for Maintenance, Survival and Vertical Transmission in Mosquitoes","volume":"98","author":"Lumley","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.smallrumres.2016.02.018","article-title":"Emergence of PPR and Its Threat to Europe","volume":"142","author":"Parida","year":"2016","journal-title":"Small Rumin. Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Mahapatra, M., Selvaraj, M., and Parida, S. (2020). Comparison of Immunogenicity and Protective Efficacy of PPR Live Attenuated Vaccines (Nigeria 75\/1 and Sungri 96) Administered by Intranasal and Subcutaneous Routes. Vaccines, 8.","DOI":"10.3390\/vaccines8020168"},{"key":"ref_8","first-page":"101","article-title":"Preliminary Results on Innocuity and Immunogenicity of an Inactivated Vaccine against Peste Des Petits Ruminants","volume":"52","author":"Ronchi","year":"2016","journal-title":"Vet. Ital."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1586\/erv.10.74","article-title":"Vaccines against Peste Des Petits Ruminants Virus","volume":"9","author":"Sen","year":"2010","journal-title":"Expert Rev. Vaccines"},{"key":"ref_10","unstructured":"Njeumi, F., Ferrari, G., Raizman, E., Diallo, A., Domenech, J., Leboucq, N., and Munstermann, S. (2015). Global Strategy for the Control and Eradication of PPR, FAO."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1007\/s12088-010-0026-9","article-title":"MVA Recombinants Expressing the Fusion and Hemagglutinin Genes of PPRV Protects Goats against Virulent Challenge","volume":"50","author":"Chandran","year":"2010","journal-title":"Indian J. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"705539","DOI":"10.3389\/fimmu.2021.705539","article-title":"Immunization With Bovine Herpesvirus-4-Based Vector Delivering PPRV-H Protein Protects Sheep from PPRV Challenge","volume":"12","author":"Rojas","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5323","DOI":"10.1016\/j.vaccine.2014.07.051","article-title":"Evaluation of Nonspreading Rift Valley Fever Virus as a Vaccine Vector Using Influenza Virus Hemagglutinin as a Model Antigen","volume":"32","author":"Oreshkova","year":"2014","journal-title":"Vaccine"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e0008942","DOI":"10.1371\/journal.pntd.0008942","article-title":"Recombinant Rift Valley Fever Viruses Encoding Bluetongue Virus (BTV) Antigens: Immunity and Efficacy Studies upon a BTV-4 Challenge","volume":"14","author":"Moreno","year":"2020","journal-title":"PLoS Negl. Trop. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1016\/j.vaccine.2023.03.055","article-title":"Efficacy of Multivalent Recombinant Herpesvirus of Turkey Vaccines against High Pathogenicity Avian Influenza, Infectious Bursal Disease, and Newcastle Disease Viruses","volume":"41","author":"Criado","year":"2023","journal-title":"Vaccine"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Boshra, H., Blyth, G.A.D., Truong, T., Kroeker, A., Kara, P., Mather, A., Wallace, D., and Babiuk, S. (2024). The Development of a Multivalent Capripoxvirus-Vectored Vaccine Candidate to Protect against Sheeppox, Goatpox, Peste Des Petits Ruminants, and Rift Valley Fever. Vaccines, 12.","DOI":"10.3390\/vaccines12070805"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/1297-9716-45-24","article-title":"Recombinant Adenovirus Expressing the Haemagglutinin of Peste Des Petits Ruminants Virus (PPRV) Protects Goats against Challenge with Pathogenic Virus; a DIVA Vaccine for PPR","volume":"45","author":"Herbert","year":"2014","journal-title":"Vet. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s13567-016-0306-4","article-title":"Determination of the Minimum Fully Protective Dose of Adenovirus-Based DIVA Vaccine against Peste Des Petits Ruminants Virus Challenge in East African Goats","volume":"47","author":"Holzer","year":"2016","journal-title":"Vet. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/0168-1702(87)90020-7","article-title":"Reactivity with Monoclonal Antibodies of Viruses from an Episode of Foot-and-Mouth Disease","volume":"8","author":"Mateu","year":"1987","journal-title":"Virus Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2157","DOI":"10.1099\/vir.0.2008\/002097-0","article-title":"T7 RNA Polymerase-Dependent and -Independent Systems for cDNA-Based Rescue of Rift Valley Fever Virus","volume":"89","author":"Habjan","year":"2008","journal-title":"J. Gen. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"275","DOI":"10.4161\/mabs.2.3.11676","article-title":"Development and Characterization of Monoclonal Antibodies against Rift Valley Fever Virus Nucleocapsid Protein Generated by DNA Immunization","volume":"2","author":"Lorenzo","year":"2010","journal-title":"MAbs"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.vaccine.2013.11.033","article-title":"Two Replication-Defective Adenoviral Vaccine Vectors for the Induction of Immune Responses to PPRV","volume":"32","author":"Rojas","year":"2014","journal-title":"Vaccine"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/0042-6822(89)90346-2","article-title":"Immunization with a Vaccinia Recombinant Expressing the F Protein Protects Rabbits from Challenge with a Lethal Dose of Rinderpest Virus","volume":"170","author":"Barrett","year":"1989","journal-title":"Virology"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Munir, M., Abubakar, M., Zohari, S., Berg, M., Munir, M., Abubakar, M., Zohari, S., and Berg, M. (2012). Serodiagnosis of Peste Des Petits Ruminants Virus. Serological Diagnosis of Certain Human, Animal and Plant Diseases, IntechOpen.","DOI":"10.5772\/36464"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/s13567-017-0482-x","article-title":"Vaccination with Recombinant Adenovirus Expressing Peste Des Petits Ruminants Virus-F or -H Proteins Elicits T Cell Responses to Epitopes That Arises during PPRV Infection","volume":"48","author":"Rojas","year":"2017","journal-title":"Vet. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.molimm.2015.02.001","article-title":"Understanding Rift Valley Fever: Contributions of Animal Models to Disease Characterization and Control","volume":"66","author":"Lorenzo","year":"2015","journal-title":"Mol. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Nair, N., Osterhaus, A.D.M.E., Rimmelzwaan, G.F., and Prajeeth, C.K. (2023). Rift Valley Fever Virus\u2014Infection, Pathogenesis and Host Immune Responses. Pathogens, 12.","DOI":"10.3390\/pathogens12091174"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5591","DOI":"10.1016\/j.vaccine.2007.02.013","article-title":"The Threat of Peste Des Petits Ruminants: Progress in Vaccine Development for Disease Control","volume":"25","author":"Diallo","year":"2007","journal-title":"Vaccine"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Wilson, W.C., Faburay, B., Trujillo, J.D., Ragan, I., Sunwoo, S.-Y., Morozov, I., Shivanna, V., Balogh, A., Urbaniak, K., and McVey, D.S. (2021). Preliminary Evaluation of a Recombinant Rift Valley Fever Virus Glycoprotein Subunit Vaccine Providing Full Protection against Heterologous Virulent Challenge in Cattle. Vaccines, 9.","DOI":"10.3390\/vaccines9070748"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e37170","DOI":"10.1371\/journal.pone.0037170","article-title":"A Novel Recombinant Peste Des Petits Ruminants-Canine Adenovirus Vaccine Elicits Long-Lasting Neutralizing Antibody Response against PPR in Goats","volume":"7","author":"Qin","year":"2012","journal-title":"PLoS ONE"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"907675","DOI":"10.3389\/fimmu.2022.907675","article-title":"Single-Dose of a Replication-Competent Adenovirus-Vectored Vaccine Provides Sterilizing Protection against Rift Valley Fever Virus Challenge","volume":"13","author":"Bian","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.virol.2017.11.015","article-title":"Comparative Evaluation of Three Capripoxvirus-Vectored Peste Des Petits Ruminants Vaccines","volume":"514","author":"Fakri","year":"2018","journal-title":"Virology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.1128\/CVI.00220-10","article-title":"Protection of Sheep against Rift Valley Fever Virus and Sheep Poxvirus with a Recombinant Capripoxvirus Vaccine","volume":"17","author":"Soi","year":"2010","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"256","DOI":"10.3389\/fvets.2020.00256","article-title":"Protection of Cattle Elicited Using a Bivalent Lumpy Skin Disease Virus-Vectored Recombinant Rift Valley Fever Vaccine","volume":"7","author":"Wallace","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"421","DOI":"10.3389\/fimmu.2018.00421","article-title":"Bovine Herpesvirus-4-Based Vector Delivering Peste Des Petits Ruminants Virus Hemagglutinin ORF Induces Both Neutralizing Antibodies and Cytotoxic T Cell Responses","volume":"9","author":"Macchi","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1186\/s12985-017-0811-8","article-title":"An Equine Herpesvirus Type 1 (EHV-1) Vector Expressing Rift Valley Fever Virus (RVFV) Gn and Gc Induces Neutralizing Antibodies in Sheep","volume":"14","author":"Said","year":"2017","journal-title":"Virol. J."},{"key":"ref_37","first-page":"1000003","article-title":"Evaluation of a Combined Peste Des Petits Ruminants and Rift Valley Fever Live Vaccine in Sheep and Goats","volume":"12","author":"Safini","year":"2021","journal-title":"J. Vaccines Vaccin."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1186\/s12985-016-0573-8","article-title":"Rift Valley Fever Virus NSs Protein Functions and the Similarity to Other Bunyavirus NSs Proteins","volume":"13","author":"Ly","year":"2016","journal-title":"Virol. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1016\/j.vaccine.2015.01.077","article-title":"Four-Segmented Rift Valley Fever Virus Induces Sterile Immunity in Sheep after a Single Vaccination","volume":"33","author":"Kant","year":"2015","journal-title":"Vaccine"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Prajapati, M., Alfred, N., Dou, Y., Yin, X., Prajapati, R., Li, Y., and Zhang, Z. (2019). Host Cellular Receptors for the Peste Des Petits Ruminant Virus. Viruses, 11.","DOI":"10.3390\/v11080729"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.virusres.2018.09.015","article-title":"Genetic Fusion of Peste Des Petits Ruminants Virus Haemagglutinin and Fusion Protein Domains to the Amino Terminal Subunit of Glycoprotein B of Bovine Herpesvirus 1 Interferes with Transport and Function of gB for BHV-1 Infectious Replication","volume":"258","author":"Osman","year":"2018","journal-title":"Virus Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"630307","DOI":"10.3389\/fimmu.2021.630307","article-title":"A Mouse Model of PPRV Infection for Elucidating Protective and Pathological Roles of Immune Cells","volume":"12","author":"Sharma","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e1010759","DOI":"10.1371\/journal.ppat.1010759","article-title":"Extracellular Vesicles Derived from PPRV-Infected Cells Enhance Signaling Lymphocyte Activation Molecular (SLAM) Receptor Expression and Facilitate Virus Infection","volume":"18","author":"Chen","year":"2022","journal-title":"PLoS Pathog."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1089\/vbz.2014.1650","article-title":"A Glycoprotein Subunit Vaccine Elicits a Strong Rift Valley Fever Virus Neutralizing Antibody Response in Sheep","volume":"14","author":"Faburay","year":"2014","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Ura, T., Takeuchi, M., Kawagoe, T., Mizuki, N., Okuda, K., and Shimada, M. (2022). Current Vaccine Platforms in Enhancing T-Cell Response. Vaccines, 10.","DOI":"10.3390\/vaccines10081367"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetimm.2013.05.002","article-title":"Recombinant Adenovirus Expressing F and H Fusion Proteins of Peste Des Petits Ruminants Virus Induces Both Humoral and Cell-Mediated Immune Responses in Goats","volume":"154","author":"Wang","year":"2013","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s13028-020-0505-x","article-title":"Paradigm Shift in the Diagnosis of Peste Des Petits Ruminants: Scoping Review","volume":"62","author":"Kinimi","year":"2020","journal-title":"Acta Vet. Scand."}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/13\/10\/1039\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T04:37:13Z","timestamp":1760157433000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/13\/10\/1039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,9]]},"references-count":47,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["vaccines13101039"],"URL":"https:\/\/doi.org\/10.3390\/vaccines13101039","relation":{},"ISSN":["2076-393X"],"issn-type":[{"type":"electronic","value":"2076-393X"}],"subject":[],"published":{"date-parts":[[2025,10,9]]}}}