{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T23:39:28Z","timestamp":1783640368023,"version":"3.55.0"},"reference-count":102,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Oropouche virus (OROV) is an emerging arthropod-borne virus in the Americas that has evolved from a pathogen historically restricted to forest environments into an increasingly important regional and international public health concern. Despite decades of documented circulation, the true burden of OROV infection remains substantially underestimated, largely because of frequent misdiagnosis and the high proportion of asymptomatic or subclinical infections. This review synthesizes current evidence on the historical emergence, epidemiology, transmission dynamics, and clinical features of OROV, with a particular focus on populations at increased risk due to biological susceptibility, environmental exposure, and limited access to healthcare. Drawing on seroepidemiological data, we demonstrate that OROV transmission is far more widespread than routine surveillance suggests and examine how factors such as age, pregnancy, immune status, underlying health conditions, occupational exposure, and healthcare accessibility interact to influence disease risk and detection. Although multiple vaccine platforms have shown promise in preclinical studies, progress toward clinical development remains constrained by limited immunological evidence, shortcomings of available animal models, diagnostic uncertainty, and structural barriers in endemic regions. We propose that future OROV vaccine development prioritize population-specific needs rather than focusing solely on technological platforms, and that effective prevention will require integrating vaccination with strengthened surveillance, improved diagnostics, and equitable delivery systems.<\/jats:p>","DOI":"10.3390\/vaccines14030267","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:10:43Z","timestamp":1773655843000},"page":"267","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Oropouche Virus (OROV) Vaccine Development for Vulnerable Populations: Epidemiological Context, Challenges and Future Directions"],"prefix":"10.3390","volume":"14","author":[{"given":"Wenrui","family":"Wu","sequence":"first","affiliation":[{"name":"Division of Natural and Applied Sciences, Duke Kunshan University, No. 8 Duke Avenue, Kunshan 215316, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8360-9537","authenticated-orcid":false,"given":"Yiu-Wing","family":"Kam","sequence":"additional","affiliation":[{"name":"Division of Natural and Applied Sciences, Duke Kunshan University, No. 8 Duke Avenue, Kunshan 215316, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e439","DOI":"10.1016\/S1473-3099(23)00740-5","article-title":"Emergence of Oropouche Fever in Latin America: A Narrative Review","volume":"24","author":"Wesselmann","year":"2024","journal-title":"Lancet Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1038\/s41541-022-00456-2","article-title":"Baseline Mapping of Oropouche Virology, Epidemiology, Therapeutics, and Vaccine Research and Development","volume":"7","author":"Files","year":"2022","journal-title":"NPJ Vaccines"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"199318","DOI":"10.1016\/j.virusres.2024.199318","article-title":"Oropouche Virus: A Neglected Global Arboviral Threat","volume":"341","author":"Zhang","year":"2024","journal-title":"Virus Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1038\/nature03959","article-title":"Genome Sequencing in Microfabricated High-Density Picolitre Reactors","volume":"437","author":"Margulies","year":"2005","journal-title":"Nature"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.virol.2019.06.013","article-title":"Evolutionary History of Simbu Serogroup Orthobunyaviruses in the Australian Episystem","volume":"535","author":"Wang","year":"2019","journal-title":"Virology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"101596","DOI":"10.1016\/j.nmni.2025.101596","article-title":"The Emergence of Oropouche Fever: A Potential New Threat?","volume":"65","author":"Srivastava","year":"2025","journal-title":"New Microbes New Infect."},{"key":"ref_7","unstructured":"Brasil, Minist\u00e9rio Da Sa\u00fade, Secretaria de Vigil\u00e2ncia Em Sa\u00fade e Ambiente (2024). NOTA T\u00c9CNICA N\u00b0 117\/2024-CGARB\/DEDT\/SVSA\/MS."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.4269\/ajtmh.16-0672","article-title":"Oropouche Virus: Clinical, Epidemiological, and Molecular Aspects of a Neglected Orthobunyavirus","volume":"96","author":"Pinheiro","year":"2017","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1590\/0074-02760150123","article-title":"Detection of Oropouche Virus Segment S in Patients and in Culex Quinquefasciatus in the State of Mato Grosso, Brazil","volume":"110","author":"Cardoso","year":"2015","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"574","DOI":"10.4269\/ajtmh.1961.10.574","article-title":"Oropouche Virus: A New Human Disease Agent from Trinidad, West Indies","volume":"10","author":"Anderson","year":"1961","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"149","DOI":"10.4269\/ajtmh.1981.30.149","article-title":"Oropouche Virus. I. A Review of Clinical, Epidemiological, and Ecological Findings","volume":"30","author":"Pinheiro","year":"1981","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"148","DOI":"10.4269\/ajtmh.1997.56.148","article-title":"Oropouche Virus Transmission in the Amazon River Basin of Peru","volume":"56","author":"Watts","year":"1997","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1111\/j.1749-6632.1994.tb19863.x","article-title":"The Emerging Epidemiology of Venezuelan Hemorrhagic Fever and Oropouche Fever in Tropical South America","volume":"740","author":"Tesh","year":"1994","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Forshey, B.M., Guevara, C., Laguna-Torres, V.A., Cespedes, M., Vargas, J., Gianella, A., Vallejo, E., Madrid, C., Aguayo, N., and Gotuzzo, E. (2010). Arboviral Etiologies of Acute Febrile Illnesses in Western South America, 2000\u20132007. PLoS Negl. Trop. Dis., 4.","DOI":"10.1371\/journal.pntd.0000787"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"710","DOI":"10.4269\/ajtmh.1998.59.710","article-title":"Epidemiology of Endemic Oropouche Virus Transmission in Upper Amazonian Peru","volume":"59","author":"Baisley","year":"1998","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1590\/S0036-46651989000400011","article-title":"1st register of an epidemic caused by Oropouche virus in the states of Maranh\u00e3o and Goi\u00e1s, Brazil","volume":"31","author":"Vasconcelos","year":"1989","journal-title":"Rev. Inst. Med. Trop."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.3201\/eid1110.050464","article-title":"Oropouche Virus Isolation, Southeast Brazil","volume":"11","author":"Nunes","year":"2005","journal-title":"Emerg. Infect. Dis."},{"key":"ref_18","first-page":"002027","article-title":"Oropouche Virus: An Emerging Orthobunyavirus","volume":"105","year":"2024","journal-title":"J. Gen. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"de Lima, R.C., Dias, H.G., de Souza, T.M.A., Familiar-Macedo, D., Ribeiro, E.D., e Corr\u00eaa, V.C., Pauvolid-Corr\u00eaa, A., de Azeredo, E.L., and dos Santos, F.B. (2024). Oropouche Virus Exposure in Febrile Patients during Chikungunya Virus Introduction in the State of Amap\u00e1, Amazon Region, Brazil. Pathogens, 13.","DOI":"10.3390\/pathogens13060469"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1590\/0074-02760160415","article-title":"Vegetation Loss and the 2016 Oropouche Fever Outbreak in Peru","volume":"112","author":"Escobar","year":"2017","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"164","DOI":"10.7883\/yoken.JJID.2019.296","article-title":"Oropouche Virus Detection in Febrile Patients\u2019 Saliva and Urine Samples in Salvador, Bahia, Brazil","volume":"73","author":"Fonseca","year":"2020","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2645","DOI":"10.1080\/22221751.2022.2136536","article-title":"Oropouche Virus as an Emerging Cause of Acute Febrile Illness in Colombia","volume":"11","author":"Ciuoderis","year":"2022","journal-title":"Emerg. Microbes Infect."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40794-024-00236-x","article-title":"Addressing the Emerging Threat of Oropouche Virus: Implications and Public Health Responses for Healthcare Systems","volume":"11","author":"Okesanya","year":"2025","journal-title":"Trop. Dis. Travel. Med. Vaccines"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2711","DOI":"10.3201\/eid2710.204760","article-title":"Outbreak of Oropouche Virus in French Guiana","volume":"27","author":"Gaillet","year":"2021","journal-title":"Emerg. Infect. Dis."},{"key":"ref_25","unstructured":"Pan American Health Organization (2024). Epidemiological Update: Oropouche in the Americas Region, 15 October 2024, PAHO. Available online: https:\/\/www.paho.org\/en\/documents\/epidemiological-update-oropouche-americas-region-15-october-2024."},{"key":"ref_26","unstructured":"Pan American Health Organization (2024). Epidemiological Alert Oropouche in the Region of the Americas: Vertical Transmission Event Under Investigation in Brazil, 17 July 2024, PAHO. Available online: https:\/\/www.paho.org\/en\/documents\/epidemiological-alert-oropouche-region-americas-vertical-transmission-event-under."},{"key":"ref_27","unstructured":"(2026, January 29). Minist\u00e9rio da Sa\u00fade Oropouche: Painel de Monitoramento Epidemiol\u00f3gico. Available online: https:\/\/app.powerbi.com\/view?r=eyJrIjoiMzc0Mzg3NjMtMzBiNy00ODhhLWJhNmItZmYzYWM4ZjUxN2Q0IiwidCI6IjlhNTU0YWQzLWI1MmItNDg2Mi1hMzZmLTg0ZDg5MWU1YzcwNSJ9."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Lorenz, C., Azevedo, T.S., Virginio, F., Aguiar, B.S., Chiaravalloti-Neto, F., and Suesdek, L. (2017). Impact of Environmental Factors on Neglected Emerging Arboviral Diseases. PLoS Neglected Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005959"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/s40249-023-01091-2","article-title":"Transmission Risk of Oropouche Fever across the Americas","volume":"12","author":"Escobar","year":"2023","journal-title":"Infect. Dis. Poverty"},{"key":"ref_30","unstructured":"Pan American Health Organization (2024). Epidemiological Update: Oropouche in the Americas Region, 6 September 2024, PAHO. Available online: https:\/\/www.paho.org\/en\/documents\/epidemiological-update-oropouche-americas-region-6-september-2024."},{"key":"ref_31","unstructured":"Pan American Health Organization (2025). Epidemiological Update: Oropouche in the Region of the Americas, 13 August 2025, PAHO. Available online: https:\/\/www.paho.org\/en\/documents\/epidemiological-update-oropouche-region-americas-13-august-2025."},{"key":"ref_32","unstructured":"Pan American Health Organization (2025). Epidemiological Update Oropouche in the Americas Region, Pan American Health Organization."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"116882","DOI":"10.1016\/j.diagmicrobio.2025.116882","article-title":"Epidemiology, Transmission Dynamics, Treatment Strategies, and Future Perspectives on Oropouche Virus","volume":"113","author":"Yoosuf","year":"2025","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Lorenz, C., de Azevedo, T.S., Sallum, M.A.M., and Chiaravalloti-Neto, F. (2025). Oropouche Fever Outbreak in Brazil: Key Factors behind the Largest Epidemic in History. PLoS ONE, 20.","DOI":"10.1371\/journal.pone.0327845"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"747","DOI":"10.4269\/ajtmh.13-0405","article-title":"Vector Competence of Culex Neavei and Culex Quinquefasciatus (Diptera: Culicidae) from Senegal for Lineages 1, 2, Koutango and a Putative New Lineage of West Nile Virus","volume":"90","author":"Fall","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1099\/jgv.0.000949","article-title":"Culex Quinquefasciatus Mosquitoes Do Not Support Replication of Zika Virus","volume":"99","author":"Marques","year":"2018","journal-title":"J. Gen. Virol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.envint.2015.09.007","article-title":"Impact of Climate Change on Human Infectious Diseases: Empirical Evidence and Human Adaptation","volume":"86","author":"Wu","year":"2016","journal-title":"Environ. Int."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"096002","DOI":"10.1289\/EHP8887","article-title":"The Complex Epidemiological Relationship between Flooding Events and Human Outbreaks of Mosquito-Borne Diseases: A Scoping Review","volume":"129","author":"Coalson","year":"2021","journal-title":"Environ. Health Perspect."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"100177","DOI":"10.1016\/j.imj.2025.100177","article-title":"Mysterious Oropouche Virus: Transmission, Symptoms, and Control","volume":"4","author":"Porwal","year":"2025","journal-title":"Infect. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3910","DOI":"10.1038\/s41598-022-07512-w","article-title":"Predicting the Possibility of African Horse Sickness (AHS) Introduction into China Using Spatial Risk Analysis and Habitat Connectivity of Culicoides","volume":"12","author":"Gao","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"117","DOI":"10.3897\/zookeys.706.13535","article-title":"A Species Checklist of the Subgenus Culicoides (Avaritia) in China, with a Description of a New Species (Diptera, Ceratopogonidae)","volume":"706","author":"Chang","year":"2017","journal-title":"ZooKeys"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1051\/parasite\/2022027","article-title":"Diversity and Seasonal Abundance of Culicoides (Diptera, Ceratopogonidae) in Shizong County, Yunnan Province, China","volume":"29","author":"Duan","year":"2022","journal-title":"Parasite"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"9127","DOI":"10.1038\/s41467-025-64446-3","article-title":"Global Invasion Patterns and Dynamics of Disease Vector Mosquitoes","volume":"16","author":"Pabst","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"taaf018","DOI":"10.1093\/jtm\/taaf018","article-title":"Travel-Associated International Spread of Oropouche Virus beyond the Amazon","volume":"32","author":"Pereira","year":"2025","journal-title":"J. Travel. Med."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Sakkas, H., Bozidis, P., Franks, A., and Papadopoulou, C. (2018). Oropouche Fever: A Review. Viruses, 10.","DOI":"10.3390\/v10040175"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2063","DOI":"10.3201\/eid1512.090917","article-title":"Oropouche Fever Outbreak, Manaus, Brazil, 2007\u20132008","volume":"15","author":"Bastos","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"732","DOI":"10.4269\/ajtmh.2012.11-0485","article-title":"Identification of Oropouche Orthobunyavirus in the Cerebrospinal Fluid of Three Patients in the Amazonas, Brazil","volume":"86","author":"Bastos","year":"2012","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.micinf.2017.11.013","article-title":"Oropouche Fever, an Emergent Disease from the Americas","volume":"20","author":"Escobar","year":"2018","journal-title":"Microbes Infect."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Wang, Z., Huang, L., Zhang, X., Zhang, X., Huang, L., Zhu, X., Long, X., Cao, D., and Li, Y. (2025). Clinical Presentation of Oropouche Virus Infection: A Systematic Review and Meta-Analysis. PLoS Negl. Trop. Dis., 19.","DOI":"10.1371\/journal.pntd.0012962"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1522","DOI":"10.1002\/jmv.23953","article-title":"Detection of Herpesvirus, Enterovirus, and Arbovirus Infection in Patients with Suspected Central Nervous System Viral Infection in the Western Brazilian Amazon","volume":"86","author":"Bastos","year":"2014","journal-title":"J. Med. Virol."},{"key":"ref_51","first-page":"100406","article-title":"Re-Emergence of Oropouche Virus as a Novel Global Threat","volume":"8","author":"Salvato","year":"2025","journal-title":"Curr. Res. Microb. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Godinho, I.P., D\u00f3ria, \u00cd.F., Rocha, V.D.M., Miranda, B.A., Salom\u00e3o, L.F.C., Stancioli, B., Paim, A.A.O., Coelho dos Reis, J.G.A., Pereira, S.H., and da Fonseca, F.G. (2025). Insights into the Expansion of Oropouche Virus in Brazil: Epidemiological and Environmental Aspects. Exp. Biol. Med., 250.","DOI":"10.3389\/ebm.2025.10647"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"116699","DOI":"10.1016\/j.diagmicrobio.2025.116699","article-title":"Potential Therapeutics and Vaccines: Current Progress and Challenges in Developing Antiviral Treatments or Vaccines for Oropouche Virus","volume":"111","author":"Vijukumar","year":"2025","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Bai, F., Denyoh, P.M.D., Urquhart, C., Shrestha, S., and Yee, D.A. (2025). A Comprehensive Review of the Neglected and Emerging Oropouche Virus. Viruses, 17.","DOI":"10.3390\/v17030439"},{"key":"ref_55","unstructured":"National Academies of Sciences, Engineering, and Medicine (2021). The Future of Nursing 2020\u20132030: Charting a Path to Achieve Health Equity, National Academies Press (US)."},{"key":"ref_56","unstructured":"National Research Council, Woolf, S.H., and Aron, L. (2013). Physical and Social Environmental Factors. U.S. Health in International Perspective: Shorter Lives, Poorer Health, National Academies Press (US)."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.immuni.2017.03.009","article-title":"Protecting the Newborn and Young Infant from Infectious Diseases: Lessons from Immune Ontogeny","volume":"46","author":"Kollmann","year":"2017","journal-title":"Immunity"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Pieren, D.K.J., Boer, M.C., and de Wit, J. (2022). The Adaptive Immune System in Early Life: The Shift Makes It Count. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.1031924"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Palacios-Pedrero, M.\u00c1., Osterhaus, A.D.M.E., Becker, T., Elbahesh, H., Rimmelzwaan, G.F., and Saletti, G. (2021). Aging and Options to Halt Declining Immunity to Virus Infections. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.681449"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e37","DOI":"10.4110\/in.2019.19.e37","article-title":"Aging and the Immune System: The Impact of Immunosenescence on Viral Infection, Immunity and Vaccine Immunogenicity","volume":"19","author":"Oh","year":"2019","journal-title":"Immune Netw."},{"key":"ref_61","first-page":"17","article-title":"Immunosenescence: Implications for Vaccination Programs in the Elderly","volume":"5","author":"Loukov","year":"2015","journal-title":"Vaccine: Dev. Ther."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.yfpn.2022.11.004","article-title":"Immunosenescence and Infectious Disease Risk Among Aging Adults","volume":"5","author":"Gray","year":"2023","journal-title":"Adv. Fam. Pract. Nurs."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Schwartz, D.A., Dashraath, P., and Baud, D. (2024). Oropouche Virus (OROV) in Pregnancy: An Emerging Cause of Placental and Fetal Infection Associated with Stillbirth and Microcephaly Following Vertical Transmission. Viruses, 16.","DOI":"10.3390\/v16091435"},{"key":"ref_64","unstructured":"Pan American Health Organization (2024). Epidemiological Alert Oropouche in the Region of the Americas: Vertical Transmission Event Under Investigation in Brazil, Pan American Health Organization."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1016\/S1473-3099(24)00516-4","article-title":"Oropouche Fever, the Mysterious Threat","volume":"24","author":"Diseases","year":"2024","journal-title":"Lancet Infect. Dis."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Agrati, C., Bartolini, B., Bordoni, V., Locatelli, F., Capobianchi, M.R., Di Caro, A., Castilletti, C., and Ippolito, G. (2023). Emerging Viral Infections in Immunocompromised Patients: A Great Challenge to Better Define the Role of Immune Response. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1147871"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Mej\u00eda, M.F.\u00c1., Shu, P.-Y., and Ji, D.-D. (2023). Accuracy of Dengue, Chikungunya, and Zika Diagnoses by Primary Healthcare Physicians in Tegucigalpa, Honduras. BMC Infect. Dis., 23.","DOI":"10.1186\/s12879-023-08346-1"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S1473-3099(24)00617-0","article-title":"Newborns with Microcephaly in Brazil and Potential Vertical Transmission of Oropouche Virus: A Case Series","volume":"25","author":"Chiang","year":"2025","journal-title":"Lancet Infect. Dis."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s12979-025-00503-1","article-title":"Delayed Viral Clearance and Altered Inflammatory Responses Affect Severity of SARS-CoV-2 Infection in Aged Mice","volume":"22","author":"Lacasse","year":"2025","journal-title":"Immun. Ageing"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Gomes Mour\u00e3o, M.P., de Melo, G.C., Nascimento, J., Mwangi, V.I., Sacchetto, L., Gardinassi, L.G., Linhares Abreu Netto, R., Mota, J., Pinto, S.D., and Tavares, M. (2025). Clinical and Laboratory Profiles of Oropouche Virus Disease from the 2024 Outbreak in Manaus, Brazilian Amazon. PLoS Negl. Trop. Dis., 19.","DOI":"10.1371\/journal.pntd.0013604"},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Louis, N. (2025). Oropouche Virus: Critical Research Priorities for This Re-Emerging Pathogen. Caribb. Med. J.","DOI":"10.48107\/CMJ.2025.03.005"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1186\/s12985-025-02945-x","article-title":"Exploring the Challenge of Diagnosing and Tracking Oropouche Virus during Brazil\u2019s Largest Recorded Dengue Epidemic in 2024","volume":"22","author":"Farias","year":"2025","journal-title":"Virol. J"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"2516","DOI":"10.1097\/MS9.0000000000003131","article-title":"The Oropouche Virus (OROV) and Need of Advanced Diagnostics to Mitigate It","volume":"87","author":"Patel","year":"2025","journal-title":"Ann. Med. Surg."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"e01629-23","DOI":"10.1128\/spectrum.01629-23","article-title":"Outbreak of Oropouche Virus in Frontier Regions in Western Amazon","volume":"12","author":"Moreira","year":"2024","journal-title":"Microbiol. Spectr."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"e01467-25","DOI":"10.1128\/mbio.01467-25","article-title":"Effective Treatment of Advanced Oropouche Virus, Rift Valley Fever Virus, and Dabie Bandavirus Infections with 4\u2032-Fluorouridine","volume":"16","author":"Westover","year":"2025","journal-title":"mBio"},{"key":"ref_76","unstructured":"World Health Organization (2026). Oropouche Virus Research and Development Roadmap, WHO R&D Blueprint."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1636","DOI":"10.1099\/vir.0.000108","article-title":"Genetic Analysis of Members of the Species Oropouche Virus and Identification of a Novel M Segment Sequence","volume":"96","author":"Hughes","year":"2015","journal-title":"J. General. Virol."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Deiana, M., Malag\u00f2, S., Mori, A., Accordini, S., Matucci, A., Passarelli Mantovani, R., Gianesini, N., Huits, R., Piubelli, C., and Gobbi, F.G. (2024). Full Genome Characterization of the First Oropouche Virus Isolate Imported in Europe from Cuba. Viruses, 16.","DOI":"10.3390\/v16101586"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"2261","DOI":"10.1016\/j.vaccine.2023.02.050","article-title":"Immunogenicity of Adjuvanted Plant-Produced SARS-CoV-2 Beta Spike VLP Vaccine in New Zealand White Rabbits","volume":"41","author":"Abolnik","year":"2023","journal-title":"Vaccine"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/S1473-3099(24)00619-4","article-title":"Re-Emergence of Oropouche Virus between 2023 and 2024 in Brazil: An Observational Epidemiological Study","volume":"25","author":"Scachetti","year":"2025","journal-title":"Lancet Infect. Dis."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"2585624","DOI":"10.1080\/21505594.2025.2585624","article-title":"Orthobunyaviruses: Epidemiology, Innate Immune Escape, and Intervention Strategies","volume":"16","author":"Li","year":"2025","journal-title":"Virulence"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"e00463-21","DOI":"10.1128\/mBio.00463-21","article-title":"Vesicular Stomatitis Virus Chimeras Expressing the Oropouche Virus Glycoproteins Elicit Protective Immune Responses in Mice","volume":"12","author":"Stubbs","year":"2021","journal-title":"mBio"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"eadx9405","DOI":"10.1126\/sciadv.adx9405","article-title":"Oropouche Virus Efficiently Replicates and Is Immunostimulatory in Vivo in Nonhuman Primate Species","volume":"11","author":"Yee","year":"2025","journal-title":"Sci. Adv."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"100456","DOI":"10.1016\/j.ijregi.2024.100456","article-title":"Oropouche Virus: A Re-Emerging Arbovirus of Clinical Significance","volume":"13","author":"Desai","year":"2024","journal-title":"IJID Reg."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/microbiolspec.DMIH2-0026-2016","article-title":"Overview of Infections in the Immunocompromised Host","volume":"4","author":"Dropulic","year":"2016","journal-title":"Microbiol. Spectr."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1002\/ana.27139","article-title":"Oropouche Virus: An Emerging Neuroinvasive Arbovirus","volume":"97","author":"Pastula","year":"2024","journal-title":"Ann. Neurol."},{"key":"ref_87","unstructured":"(2026, January 06). From Warehouse to Remote Indigenous Communities: The Journey of Vaccines in Brazil. Available online: https:\/\/www.who.int\/news-room\/feature-stories\/detail\/from-warehouse-to-remote-indigenous-communities-the-journey-of-vaccines-in-brazil."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Hoyt, J., Okello, G., Bange, T., Kariuki, S., Jalloh, M.F., Webster, J., and Hill, J. (2023). RTS,S\/AS01 Malaria Vaccine Pilot Implementation in Western Kenya: A Qualitative Longitudinal Study to Understand Immunisation Barriers and Optimise Uptake. BMC Public Health, 23.","DOI":"10.1186\/s12889-023-17194-2"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"e014719","DOI":"10.1136\/bmjgh-2023-014719","article-title":"Policy Uptake and Implementation of the RTS,S\/AS01 Malaria Vaccine in Sub-Saharan African Countries: Status 2 Years Following the WHO Recommendation","volume":"9","author":"Osoro","year":"2024","journal-title":"BMJ Glob. Health"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/s13187-014-0680-4","article-title":"Promotora Outreach, Education and Navigation Support for HPV Vaccination to Hispanic Women with Unvaccinated Daughters","volume":"30","author":"Mojica","year":"2015","journal-title":"J. Cancer Educ."},{"key":"ref_91","unstructured":"World Health Organization (2025, November 23). Malaria: The Malaria Vaccine Implementation Programme (MVIP). Available online: https:\/\/www.who.int\/news-room\/questions-and-answers\/item\/malaria-vaccine-implementation-programme."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Ghattas, M., Dwivedi, G., Lavertu, M., and Alameh, M.-G. (2021). Vaccine Technologies and Platforms for Infectious Diseases: Current Progress, Challenges, and Opportunities. Vaccines, 9.","DOI":"10.3390\/vaccines9121490"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"3132","DOI":"10.1080\/21645515.2016.1222341","article-title":"The Safety of Maternal Immunization","volume":"12","author":"Regan","year":"2016","journal-title":"Hum. Vaccin. Immunother."},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Cinicola, B., Conti, M.G., Terrin, G., Sgrulletti, M., Elfeky, R., Carsetti, R., Fernandez Salinas, A., Piano Mortari, E., Brindisi, G., and De Curtis, M. (2021). The Protective Role of Maternal Immunization in Early Life. Front. Pediatr., 9.","DOI":"10.3389\/fped.2021.638871"},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Niewiesk, S. (2014). Maternal Antibodies: Clinical Significance, Mechanism of Interference with Immune Responses, and Possible Vaccination Strategies. Front. Immunol., 5.","DOI":"10.3389\/fimmu.2014.00446"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"2517931","DOI":"10.1080\/21645515.2025.2517931","article-title":"Recent Developments of Vaccines for Older Adults: Adjuvants and Beyond","volume":"21","author":"Pangrazzi","year":"2025","journal-title":"Hum. Vaccines Immunother."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1038\/s41541-024-00874-4","article-title":"Insights into Vaccines for Elderly Individuals: From the Impacts of Immunosenescence to Delivery Strategies","volume":"9","author":"Hou","year":"2024","journal-title":"NPJ Vaccines"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.smim.2018.10.010","article-title":"Vaccination in the Elderly: The Challenge of Immune Changes with Aging","volume":"40","author":"Ciabattini","year":"2018","journal-title":"Semin. Immunol."},{"key":"ref_99","doi-asserted-by":"crossref","unstructured":"Song, R., Yang, C., Li, Q., Wang, J., Chen, J., Sun, K., Lv, H., Yang, Y., Liang, J., and Ye, Q. (2023). Durability of Immune Response after SARS-CoV-2 Vaccination in Patients with Chronic Liver Disease. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1200198"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"108021","DOI":"10.1016\/j.intimp.2021.108021","article-title":"Administration of COVID-19 Vaccines in Immunocompromised Patients","volume":"99","author":"Negahdaripour","year":"2021","journal-title":"Int. Immunopharmacol."},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Leong, Y.Z., and Leong, W.Y. (2026). Sustainable Cold-Chain Logistics for Vaccine and Blood Supply in East Malaysia. Eng. Proc., 129.","DOI":"10.3390\/engproc2026129015"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"75","DOI":"10.2471\/BLT.12.110700","article-title":"Vaccine Presentation and Packaging Advisory Group: A Forum for Reaching Consensus on Vaccine Product Attributes","volume":"91","author":"Mansoor","year":"2013","journal-title":"Bull. World Health Organ."}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/14\/3\/267\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:47:34Z","timestamp":1773658054000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/14\/3\/267"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,16]]},"references-count":102,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,3]]}},"alternative-id":["vaccines14030267"],"URL":"https:\/\/doi.org\/10.3390\/vaccines14030267","relation":{},"ISSN":["2076-393X"],"issn-type":[{"value":"2076-393X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,16]]}}}