{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:42:07Z","timestamp":1792093327008,"version":"4.4.0"},"reference-count":64,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005046","name":"Heilongjiang Provincial Natural Science Foundation of China","doi-asserted-by":"crossref","award":["LH2022C070"],"award-info":[{"award-number":["LH2022C070"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Porcine enteric coronaviruses (PECs), including porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV), remain major causes of neonatal diarrhea, dehydration, mortality, and economic loss in swine production. Despite substantial progress in vaccine development, durable field protection is still inconsistent. In this narrative review, this narrative review synthesizes current knowledge on PEC vaccine design from three connected perspectives: antigenic breadth, mucosal immunity, and translational evaluation. The economic and virological context of PEC vaccine development is first summarized, including the recurrent production burden of PECs, coronavirus genome organization, structural proteins, and the central role of the spike protein in receptor engagement, membrane fusion, and neutralizing antibody induction. Key issues are then discussed, including how spike diversity, conformational stability, epitope accessibility, glycan shielding, and antigen matching influence protective breadth; why intestinal secretory IgA, mucosal immune-cell trafficking, local memory responses, and lactogenic immunity should be prioritized as biologically relevant endpoints; and how delivery route, adjuvant selection, and platform design shape response quality. Current evidence on recombinant protein, viral-vectored, nanoparticle, virus-like particle, probiotic, plant-derived, and mRNA-based approaches is compared with attention to both promise and current evidentiary and translational limitations. The available literature suggests that future progress in PEC vaccinology is likely to depend less on platform novelty alone than on integrated vaccine designs that align antigen selection, mucosal delivery, maternal\u2013neonatal protection, heterologous challenge, manufacturability, and field applicability.<\/jats:p>","DOI":"10.3390\/vaccines14060498","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T12:45:08Z","timestamp":1780404308000},"page":"498","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Next-Generation Vaccine Design for Porcine Enteric Coronaviruses: Aligning Antigenic Breadth, Mucosal Immunity, and Translational Evaluation"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2872-0088","authenticated-orcid":false,"given":"Fanzhi","family":"Kong","sequence":"first","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Sartu District, Daqing 163319, China"},{"name":"XL Future AgriTech, National University Science Park, Northeast Petroleum University, No. 38 Huoju Xinjie, Daqing High-Tech Zone, Daqing 163000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nannan","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Sartu District, Daqing 163319, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuxuan","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Sartu District, Daqing 163319, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufeng","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Sartu District, Daqing 163319, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kong, F., Jia, H., Xiao, Q., Fang, L., and Wang, Q. (2023). Prevention and Control of Swine Enteric Coronaviruses in China: A Review of Vaccine Development and Application. Vaccines, 12.","DOI":"10.3390\/vaccines12010011"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s11259-021-09808-0","article-title":"Porcine enteric coronaviruses: An updated overview of the pathogenesis, prevalence, and diagnosis","volume":"45","author":"Liu","year":"2021","journal-title":"Vet. Res. Commun."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Park, J.E. (2024). Porcine Epidemic Diarrhea: Insights and Progress on Vaccines. Vaccines, 12.","DOI":"10.20944\/preprints202401.1467.v1"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1007\/s12250-021-00364-0","article-title":"Porcine Coronaviruses: Overview of the State of the Art","volume":"36","year":"2021","journal-title":"Virol. Sin."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ibrahim, Y.M., Liu, C., Yu, Y., Yang, L., Chen, Q., Ma, W., Werid, G.M., Li, S., Luo, J., and Gao, S. (2026). Swine Enteric Coronaviruses: An Updated Overview of Epidemiology, Diagnosis, Prevention, and Control. Animals, 16.","DOI":"10.3390\/ani16030458"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Kong, F., Wang, Q., Kenney, S.P., Jung, K., Vlasova, A.N., and Saif, L.J. (2022). Porcine Deltacoronaviruses: Origin, Evolution, Cross-Species Transmission and Zoonotic Potential. Pathogens, 11.","DOI":"10.3390\/pathogens11010079"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5111","DOI":"10.2527\/jas.2015-9136","article-title":"Assessment of the economic impacts of porcine epidemic diarrhea virus in the United States","volume":"93","author":"Schulz","year":"2015","journal-title":"J. Anim. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1038\/s43016-021-00362-1","article-title":"African swine fever outbreaks in China led to gross domestic product and economic losses","volume":"2","author":"You","year":"2021","journal-title":"Nat. Food"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"106627","DOI":"10.1016\/j.prevetmed.2025.106627","article-title":"Economic impact of productivity losses attributable to porcine reproductive and respiratory syndrome virus in United States pork production, 2016\u20132020","volume":"244","author":"Osemeke","year":"2025","journal-title":"Prev. Vet. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e01301-20","DOI":"10.1128\/JVI.01301-20","article-title":"Cryo-electron Microscopy Structure of the Swine Acute Diarrhea Syndrome Coronavirus Spike Glycoprotein Provides Insights into Evolution of Unique Coronavirus Spike Proteins","volume":"94","author":"Guan","year":"2020","journal-title":"J. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.str.2020.12.003","article-title":"Structure and immune recognition of the porcine epidemic diarrhea virus spike protein","volume":"29","author":"Kirchdoerfer","year":"2021","journal-title":"Structure"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e01556-17","DOI":"10.1128\/JVI.01556-17","article-title":"Cryo-Electron Microscopy Structure of Porcine Deltacoronavirus Spike Protein in the Prefusion State","volume":"92","author":"Shang","year":"2018","journal-title":"J. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e00923-19","DOI":"10.1128\/JVI.00923-19","article-title":"The 3.1-Angstrom Cryo-electron Microscopy Structure of the Porcine Epidemic Diarrhea Virus Spike Protein in the Prefusion Conformation","volume":"93","author":"Wrapp","year":"2019","journal-title":"J. Virol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0165-2427(96)05702-9","article-title":"Mucosal immunity: An overview and studies of enteric and respiratory coronavirus infections in a swine model of enteric disease","volume":"54","author":"Saif","year":"1996","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/0165-2427(94)90124-4","article-title":"Immunity to transmissible gastroenteritis virus and porcine respiratory coronavirus infections in swine","volume":"43","author":"Saif","year":"1994","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3980","DOI":"10.4049\/jimmunol.152.8.3980","article-title":"Contribution of antibody-secreting cells induced in mucosal lymphoid tissues of pigs inoculated with respiratory or enteric strains of coronavirus to immunity against enteric coronavirus challenge","volume":"152","author":"VanCott","year":"1994","journal-title":"J. Immunol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.coviro.2018.12.001","article-title":"Emerging and re-emerging coronaviruses in pigs","volume":"34","author":"Wang","year":"2019","journal-title":"Curr. Opin. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3230453","DOI":"10.1155\/tbed\/3230453","article-title":"Design of Mucosal Vaccines Against Swine Enteric Coronaviruses: From Antigen Delivery to Immune Activation","volume":"2025","author":"Pan","year":"2025","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/978-3-032-22340-1_2","article-title":"Basic Virology","volume":"1511","author":"Veenstra","year":"2026","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Luo, H., Liang, Z., Lin, J., Wang, Y., Liu, Y., Mei, K., Zhao, M., and Huang, S. (2024). Research progress of porcine epidemic diarrhea virus S protein. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1396894"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1038\/s41392-023-01472-x","article-title":"Targetable elements in SARS-CoV-2 S2 subunit for the design of pan-coronavirus fusion inhibitors and vaccines","volume":"8","author":"Guo","year":"2023","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1038\/s41467-024-45404-x","article-title":"Prefusion-stabilized SARS-CoV-2 S2-only antigen provides protection against SARS-CoV-2 challenge","volume":"15","author":"Hsieh","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1126\/science.abb9983","article-title":"Site-specific glycan analysis of the SARS-CoV-2 spike","volume":"369","author":"Watanabe","year":"2020","journal-title":"Science"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5330","DOI":"10.1038\/s41467-024-49693-0","article-title":"Neutralizing antibodies reveal cryptic vulnerabilities and interdomain crosstalk in the porcine deltacoronavirus spike protein","volume":"15","author":"Du","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e0095823","DOI":"10.1128\/jvi.00958-23","article-title":"A novel recombinant S-based subunit vaccine induces protective immunity against porcine deltacoronavirus challenge in piglets","volume":"97","author":"Li","year":"2023","journal-title":"J. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Wang, Y., Song, J., Deng, X., Wang, J., Zhang, M., Liu, Y., Tang, P., Liu, H., Zhou, Y., and Tong, G. (2024). Nanoparticle vaccines based on the receptor binding domain of porcine deltacoronavirus elicit robust protective immune responses in mice. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1328266"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Chen, Y., Zhou, J., Wang, X., Ma, L., Li, J., Yang, L., Yuan, H., Pang, D., and Ouyang, H. (2022). Porcine Epidemic Diarrhea Virus: An Updated Overview of Virus Epidemiology, Virulence Variation Patterns and Virus-Host Interactions. Viruses, 14.","DOI":"10.3390\/v14112434"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.3892\/etm.2020.8828","article-title":"Comparison of oral and nasal immunization with inactivated porcine epidemic diarrhea virus on intestinal immunity in piglets","volume":"20","author":"Zhang","year":"2020","journal-title":"Exp. Ther. Med."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Langel, S.N., Paim, F.C., Alhamo, M.A., Buckley, A., Van Geelen, A., Lager, K.M., Vlasova, A.N., and Saif, L.J. (2019). Stage of Gestation at Porcine Epidemic Diarrhea Virus Infection of Pregnant Swine Impacts Maternal Immunity and Lactogenic Immune Protection of Neonatal Suckling Piglets. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.00727"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e0257421","DOI":"10.1128\/spectrum.02574-21","article-title":"Systemic Homologous Neutralizing Antibodies Are Inadequate for the Evaluation of Vaccine Protective Efficacy against Coinfection by High Virulent PEDV and PRRSV","volume":"10","author":"Qiu","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s13567-020-00880-z","article-title":"Systemic and intestinal porcine epidemic diarrhea virus-specific antibody response and distribution of antibody-secreting cells in experimentally infected conventional pigs","volume":"52","author":"Suda","year":"2021","journal-title":"Vet. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1111\/j.0105-2896.2005.00292.x","article-title":"Involvement of intestinal dendritic cells in oral tolerance, immunity to pathogens, and inflammatory bowel disease","volume":"206","author":"Kelsall","year":"2005","journal-title":"Immunol. Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.addr.2017.04.008","article-title":"Current state and challenges in developing oral vaccines","volume":"114","author":"Sharpe","year":"2017","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1862531","DOI":"10.1155\/2019\/1862531","article-title":"Manipulation of Intestinal Antiviral Innate Immunity and Immune Evasion Strategies of Porcine Epidemic Diarrhea Virus","volume":"2019","author":"Du","year":"2019","journal-title":"BioMed Res. Int."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1942","DOI":"10.3748\/wjg.v29.i13.1942","article-title":"Interferon-lambda: New role in intestinal symptoms of COVID-19","volume":"29","author":"Pan","year":"2023","journal-title":"World J. Gastroenterol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"9","DOI":"10.4049\/jimmunol.1601775","article-title":"Inducing Mucosal IgA: A Challenge for Vaccine Adjuvants and Delivery Systems","volume":"199","author":"Boyaka","year":"2017","journal-title":"J. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/nri2322","article-title":"The regulation of IgA class switching","volume":"8","author":"Cerutti","year":"2008","journal-title":"Nat. Rev. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1038\/mi.2011.37","article-title":"Regulation of the polymeric immunoglobulin receptor and IgA transport: New advances in environmental factors that stimulate pIgR expression and its role in mucosal immunity","volume":"4","author":"Johansen","year":"2011","journal-title":"Mucosal. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Wei, H., and Wang, J.Y. (2021). Role of Polymeric Immunoglobulin Receptor in IgA and IgM Transcytosis. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22052284"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Su, K., Wang, Y., Yuan, C., Zhang, Y., Li, Y., Li, T., and Song, Q. (2023). Intranasally inoculated bacterium-like particles displaying porcine epidemic diarrhea virus S1 protein induced intestinal mucosal immune response in mice. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1269409"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Clancy AM, R. (2023). The Common Mucosal System Fifty Years on: From Cell Traffic in the Rabbit to Immune Resilience to SARS-CoV-2 Infection by Shifting Risk within Normal and Disease Populations. Vaccines, 11.","DOI":"10.20944\/preprints202307.0352.v1"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1126\/science.1132742","article-title":"Generation of gut-homing IgA-secreting B cells by intestinal dendritic cells","volume":"314","author":"Mora","year":"2006","journal-title":"Science"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1053\/j.gastro.2015.10.046","article-title":"Leukocyte Trafficking to the Small Intestine and Colon","volume":"150","author":"Habtezion","year":"2016","journal-title":"Gastroenterology"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Huang, Y., Xu, Z., Gu, S., Nie, M., Wang, Y., Zhao, J., Li, F., Deng, H., Huang, J., and Sun, X. (2022). The recombinant pseudorabies virus expressing porcine deltacoronavirus spike protein is safe and effective for mice. BMC Vet. Res., 18.","DOI":"10.1186\/s12917-021-03115-1"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1016\/j.virs.2025.12.007","article-title":"An adenovirus-vectored recombinant vaccine confers passive immunity protection from swine acute diarrhea syndrome coronavirus challenge in neonatal mice","volume":"40","author":"Zhang","year":"2025","journal-title":"Virol. Sin."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Niu, X., and Wang, Q. (2022). Prevention and Control of Porcine Epidemic Diarrhea: The Development of Recombination-Resistant Live Attenuated Vaccines. Viruses, 14.","DOI":"10.3390\/v14061317"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1017\/S1466252317000123","article-title":"Historical and contemporary aspects of maternal immunity in swine","volume":"19","author":"Poonsuk","year":"2018","journal-title":"Anim. Health Res. Rev."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Li, L., Yin, S., Zhou, J., Zhang, L., Teng, Z., Qiao, L., Wang, Y., Yu, J., Zang, H., and Ding, Y. (2024). Spike 1 trimer, a nanoparticle vaccine against porcine epidemic diarrhea virus induces protective immunity challenge in piglets. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1386136"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s13567-025-01695-6","article-title":"Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein","volume":"57","author":"Su","year":"2025","journal-title":"Vet. Res."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Yang, M., Xie, D., Ji, W., Zhu, S.J., and Zhou, Y. (2025). Oral Delivery of Lactococcus lactis Expressing Full-Length S Protein via Alginate-Chitosan Capsules Induces Immune Protection Against PEDV Infection in Mice. Vaccines, 13.","DOI":"10.3390\/vaccines13040421"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e0053524","DOI":"10.1128\/jvi.00535-24","article-title":"A spike-based mRNA vaccine that induces durable and broad protection against porcine deltacoronavirus in piglets","volume":"98","author":"Li","year":"2024","journal-title":"J. Virol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e0084925","DOI":"10.1128\/jvi.00849-25","article-title":"Single self-cleaving mRNA vaccine expressing multiple viral structural proteins elicits robust immune responses and protects nursing piglets against PDCoV infection","volume":"99","author":"Yu","year":"2025","journal-title":"J. Virol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"110150","DOI":"10.1016\/j.virol.2024.110150","article-title":"A novel virus-like particles vaccine induces broad immune protective against deltacoronavirus in piglets","volume":"597","author":"Zhang","year":"2024","journal-title":"Virology"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/s41392-023-01408-5","article-title":"Viral vectored vaccines: Design, development, preventive and therapeutic applications in human diseases","volume":"8","author":"Wang","year":"2023","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1111\/jphp.12353","article-title":"Recombinant protein subunit vaccine synthesis in microbes: A role for yeast?","volume":"67","author":"Bill","year":"2015","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Cid, R., and Bolivar, J. (2021). Platforms for Production of Protein-Based Vaccines: From Classical to Next-Generation Strategies. Biomolecules, 11.","DOI":"10.3390\/biom11081072"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.virusres.2016.05.016","article-title":"Lactogenic immunity and vaccines for porcine epidemic diarrhea virus (PEDV): Historical and current concepts","volume":"226","author":"Langel","year":"2016","journal-title":"Virus Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"e0130924","DOI":"10.1128\/jvi.01309-24","article-title":"Efficacy evaluation of a bivalent subunit vaccine against epidemic PEDV heterologous strains with low cross-protection","volume":"98","author":"Song","year":"2024","journal-title":"J. Virol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Won, H., Lim, J., Noh, Y.H., Yoon, I., and Yoo, H.S. (2020). Efficacy of Porcine Epidemic Diarrhea Vaccines: A Systematic Review and Meta-Analysis. Vaccines, 8.","DOI":"10.3390\/vaccines8040642"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Hou, X., Jiang, X., Jiang, Y., Tang, L., Xu, Y., Qiao, X., Min, L., Wen, C., Ma, G., and Li, Y. (2018). Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets. Viruses, 10.","DOI":"10.3390\/v10030106"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Sohn, E.J., Kang, H., Min, K., Park, M., Kim, J.H., Seo, H.W., Lee, S.J., Kim, H., Tark, D., and Cho, H.S. (2023). A Plant-Derived Maternal Vaccine against Porcine Epidemic Diarrhea Protects Piglets through Maternally Derived Immunity. Vaccines, 11.","DOI":"10.3390\/vaccines11050965"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e02958-23","DOI":"10.1128\/mbio.02958-23","article-title":"PEDV-spike-protein-expressing mRNA vaccine protects piglets against PEDV challenge","volume":"15","author":"Zhao","year":"2024","journal-title":"mBio"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Zheng, D., Wang, X., Ju, N., Wang, Z., Sui, L., Wang, L., Qiao, X., Cui, W., Jiang, Y., and Zhou, H. (2021). Immune Responses in Pregnant Sows Induced by Recombinant Lactobacillus johnsonii Expressing the COE Protein of Porcine Epidemic Diarrhea Virus Provide Protection for Piglets against PEDV Infection. Viruses, 14.","DOI":"10.3390\/v14010007"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2014296","DOI":"10.1155\/tbed\/2014296","article-title":"Next-Generation Vaccines for Co-Circulating PEDV and TGEV: Integrating Nucleic Acid Platforms, Mucosal Delivery, and AI-Driven Antigen Design","volume":"2025","author":"Hu","year":"2025","journal-title":"Transbound. Emerg. Dis."}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/14\/6\/498\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T04:44:43Z","timestamp":1780548283000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/14\/6\/498"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,2]]},"references-count":64,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["vaccines14060498"],"URL":"https:\/\/doi.org\/10.3390\/vaccines14060498","relation":{},"ISSN":["2076-393X"],"issn-type":[{"value":"2076-393X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,2]]}}}