{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T09:08:13Z","timestamp":1789549693403,"version":"build-2803163510"},"reference-count":20,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Development Programme of Jilin Province","award":["20220204136YY"],"award-info":[{"award-number":["20220204136YY"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Background: Rabies is a nearly 100% fatal zoonosis, and a rabies virus-neutralizing antibody (RVNA) titre of \u22650.5 IU\/mL is the recognized correlate of protection, making post-vaccination serological monitoring essential. However, the gold-standard methods (RFFIT and FAVN) are time-consuming, require live virus, and are unsuitable for large-scale surveillance, while current ELISA kits often show poor concordance with neutralization tests. Methods: In this study, we established a chemiluminescence immunoassay (CLIA) employing magnetic beads coated with recombinant RABV glycoprotein (short-chain) and an acridinium ester-labeled rabbit anti-dog IgG. Results: The assay exhibited a detection limit of 0.24 IU\/mL. Its performance was validated with 250 canine anti-RABV serum samples against the FAVN. The CLIA and FAVN results were strongly correlated (r = 0.9636, p &lt; 0.0001), with a Deming slope of 0.96 (95% confidence interval: 0.92\u20131.00). The assay achieved a diagnostic sensitivity of 94.4% and a specificity of 97.1%. It demonstrated high accuracy (relative bias within \u00b110%), good repeatability (intra- and inter-assay coefficients of variation &lt; 8%), and acceptable specificity, showing no cross-reactivity with canine parvovirus, canine distemper virus, or canine parainfluenza virus antibodies. Conclusions: This CLIA kit eliminates the need for live virus, yields quantitative results within 30 min, and offers simple operation and high-throughput capacity, making it a reliable and practical tool for rabies immune monitoring and scientific prevention and control.<\/jats:p>","DOI":"10.3390\/vaccines14090814","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T07:58:59Z","timestamp":1789545539000},"page":"814","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Recombinant RABV-G-Based Chemiluminescence Immunoassay for Quantitative Detection of Rabies Virus Antibodies"],"prefix":"10.3390","volume":"14","author":[{"given":"Yan","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Public Health, Beihua University, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Jilin Tongchuang Investment Co., Ltd., Changchun 130616, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Yanan","family":"Han","sequence":"additional","affiliation":[{"name":"College of Basic Medicine, Beihua University, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"software","vocabulary":"credit"}]},{"given":"Na","family":"Feng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jilin Province for Zoonoses Prevention and Control, State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Changchun 130122, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"}]},{"given":"Ye","family":"Feng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Weiyao","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Public Health, Beihua University, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e0202122","DOI":"10.1128\/mbio.02021-22","article-title":"Decoding the Role of Temperature in RNA Virus Infections","volume":"13","author":"Bisht","year":"2022","journal-title":"mBio"},{"key":"ref_2","unstructured":"World Health Organization (2013). 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