{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:21:54Z","timestamp":1790083314763,"version":"4.0.1"},"reference-count":53,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,1,19]],"date-time":"2020-01-19T00:00:00Z","timestamp":1579392000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Pharmaq AS","award":["Agreement PHARMAQ-CSIC"],"award-info":[{"award-number":["Agreement PHARMAQ-CSIC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Infestation with the salmon louse Lepeophtheirus salmonis (Copepoda, Caligidae) affects Atlantic salmon (Salmo salar L.) production in European aquaculture. Furthermore, high levels of salmon lice in farms significantly increase challenge pressure against wild salmon populations. Currently, available control methods for salmon louse have limitations, and vaccination appears as an attractive, environmentally sound strategy. In this study, we addressed one of the main limitations for vaccine development, the identification of candidate protective antigens. Based on recent advances in tick vaccine research, herein, we targeted the salmon louse midgut function and blood digestion for the identification of candidate target proteins for the control of ectoparasite infestations. The results of this translational approach resulted in the identification and subsequent evaluation of the new candidate protective antigens, putative Toll-like receptor 6 (P30), and potassium chloride, and amino acid transporter (P33). Vaccination with these antigens provided protection in Atlantic salmon by reducing adult female (P33) or chalimus II (P30) sea lice infestations. These results support the development of vaccines for the control of sea lice infestations.<\/jats:p>","DOI":"10.3390\/vaccines8010032","type":"journal-article","created":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T04:27:09Z","timestamp":1579494429000},"page":"32","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Vaccination with Ectoparasite Proteins Involved in Midgut Function and Blood Digestion Reduces Salmon Louse Infestations"],"prefix":"10.3390","volume":"8","author":[{"given":"Marinela","family":"Contreras","sequence":"first","affiliation":[{"name":"SaBio, Instituto de Investigaci\u00f3n en Recursos Cineg\u00e9ticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s\/n, 13005 Ciudad Real, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marius","family":"Karlsen","sequence":"additional","affiliation":[{"name":"Pharmaq AS, P.O. Box 267, Skoyen, N-0213 Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4172-9079","authenticated-orcid":false,"given":"Margarita","family":"Villar","sequence":"additional","affiliation":[{"name":"SaBio, Instituto de Investigaci\u00f3n en Recursos Cineg\u00e9ticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s\/n, 13005 Ciudad Real, Spain"},{"name":"Biochemistry Section, Faculty of Science and Chemical Technologies, and Regional Centre for Biomedical Research (CRIB), University of Castilla-La Mancha, 13071 Ciudad Real, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Olsen","sequence":"additional","affiliation":[{"name":"Pharmaq AS, P.O. Box 267, Skoyen, N-0213 Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Leknes","sequence":"additional","affiliation":[{"name":"Pharmaq AS, P.O. Box 267, Skoyen, N-0213 Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anette","family":"Furevik","sequence":"additional","affiliation":[{"name":"Pharmaq AS, P.O. Box 267, Skoyen, N-0213 Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Yttredal","sequence":"additional","affiliation":[{"name":"Pharmaq AS, P.O. Box 267, Skoyen, N-0213 Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3913-7410","authenticated-orcid":false,"given":"Haitham","family":"Tartor","sequence":"additional","affiliation":[{"name":"Norwegian Veterinary Institute, 0106 Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soren","family":"Grove","sequence":"additional","affiliation":[{"name":"Norwegian Veterinary Institute, 0106 Oslo, Norway"},{"name":"Institute of Marine Research, 5005 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6999-9735","authenticated-orcid":false,"given":"Pilar","family":"Alberdi","sequence":"additional","affiliation":[{"name":"SaBio, Instituto de Investigaci\u00f3n en Recursos Cineg\u00e9ticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s\/n, 13005 Ciudad Real, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bjorn","family":"Brudeseth","sequence":"additional","affiliation":[{"name":"Pharmaq AS, P.O. Box 267, Skoyen, N-0213 Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7383-9649","authenticated-orcid":false,"given":"Jos\u00e9","family":"de la Fuente","sequence":"additional","affiliation":[{"name":"SaBio, Instituto de Investigaci\u00f3n en Recursos Cineg\u00e9ticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s\/n, 13005 Ciudad Real, Spain"},{"name":"Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1038\/nature06536","article-title":"Global trends in emerging infectious diseases","volume":"451","author":"Jones","year":"2008","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1111\/jfd.12061","article-title":"Salmon lice--impact on wild salmonids and salmon aquaculture","volume":"36","author":"Torrissen","year":"2013","journal-title":"J. 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