{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T01:01:21Z","timestamp":1773795681708,"version":"3.50.1"},"reference-count":63,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,3,26]],"date-time":"2020-03-26T00:00:00Z","timestamp":1585180800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Group B Streptococcus (GBS) is the primary etiological agent of sepsis and meningitis in newborns and is associated with premature birth and stillbirth. The development of a licensed vaccine is one of the pending challenges for the World Health Organization. Previously, we showed that oral immunization with surface immune protein (SIP) decreases vaginal colonization of GBS and generates functional opsonizing antibodies, which was determined by opsonophagocytic assays (OPA) in vitro. We also showed that the protein has an adjuvant vaccine profile. Therefore, an oral vaccine based on SIP may be an attractive alternative to employ in the development of new vaccines against GBS. Lactococcus lactis is a highlighted oral vaccine probiotic inducer of the mucosal immune response. This bacterium could serve as an antigen-delivering vehicle for the development of an edible vaccine and has been used in clinical trials. In this study, we showed that an oral vaccine with a recombinant L. lactis strain secreting SIP from GBS (rL. lactis-SIP) can induce protective humoral and cellular immunity in an experimental model of GBS vaginal colonization in C57BL\/6 mice. Mice immunized with rL. lactis-SIP were protected against clinical symptoms and bacterial colonization after GBS vaginal colonization. Our rL. lactis-SIP vaccine also induces an increase of immunoglobulin G (IgG) and immunoglobulin A (IgA) specifically against SIP. The adoptive transfer of serum from vaccinated mice to na\u00efve mice generated protection against GBS vaginal colonization. Moreover, the rL. lactis-SIP strain induces the activation of SIP-specific T cells, which could decrease GBS vaginal colonization and generate protective antibodies when transferred to other mice. Our experimental observations strongly support the notion that rL. lactis-SIP induces protective humoral and cellular immunity and could be considered as a novel alternative in the development of vaccines for GBS.<\/jats:p>","DOI":"10.3390\/vaccines8020146","type":"journal-article","created":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T10:29:38Z","timestamp":1585650578000},"page":"146","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Mucosal Vaccination with Lactococcus lactis-Secreting Surface Immunological Protein Induces Humoral and Cellular Immune Protection against Group B Streptococcus in a Murine Model"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5677-920X","authenticated-orcid":false,"given":"Diego A.","family":"Diaz-Dinamarca","sequence":"first","affiliation":[{"name":"Secci\u00f3n de Biotecnolog\u00eda, Instituto de Salud P\u00fablica de Chile, Santiago 780050, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Hernandez","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Biotecnolog\u00eda, Instituto de Salud P\u00fablica de Chile, Santiago 780050, Chile"},{"name":"Millennium Institute of Immunology and Immunotherapy, Departamento de Gen\u00e9tica Molecular y Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile, Santiago 8380453, Chile"},{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Facultad de Ciencias Qu\u00edmicas y Farmaceuticas, Universidad de Chile, Independencia, Santiago 8380492, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel F.","family":"Escobar","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Biotecnolog\u00eda, Instituto de Salud P\u00fablica de Chile, Santiago 780050, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel A.","family":"Soto","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Biotecnolog\u00eda, Instituto de Salud P\u00fablica de Chile, Santiago 780050, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo A.","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Biotecnolog\u00eda, Instituto de Salud P\u00fablica de Chile, Santiago 780050, Chile"},{"name":"Millennium Institute of Immunology and Immunotherapy, Departamento de Gen\u00e9tica Molecular y Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile, Santiago 8380453, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas F.","family":"Badilla","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Biotecnolog\u00eda, Instituto de Salud P\u00fablica de Chile, Santiago 780050, Chile"},{"name":"Millennium Institute of Immunology and Immunotherapy, Departamento de Gen\u00e9tica Molecular y Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile, Santiago 8380453, Chile"},{"name":"Escuela de Biotecnolog\u00eda, Facultad de Ciencias, Universidad Santo Tomas, Santiago 8320000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo A.","family":"Manzo","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Biotecnolog\u00eda, Instituto de Salud P\u00fablica de Chile, Santiago 780050, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4086-6867","authenticated-orcid":false,"given":"Flavio","family":"Carri\u00f3n","sequence":"additional","affiliation":[{"name":"Programa de Inmunolog\u00eda Traslacional, Facultad de Medicina, Cl\u00ednica Alemana Universidad del Desarrollo, Santiago 7610315, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexis M.","family":"Kalergis","sequence":"additional","affiliation":[{"name":"Millennium Institute of Immunology and Immunotherapy, Departamento de Gen\u00e9tica Molecular y Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile, Santiago 8380453, Chile"},{"name":"Departamento de Endocrinolog\u00eda, Facultad de Medicina, Pontificia Universidad Cat\u00f3lica de Chile, Santiago 8330077, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7346-9043","authenticated-orcid":false,"given":"Abel E.","family":"Vasquez","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Biotecnolog\u00eda, Instituto de Salud P\u00fablica de Chile, Santiago 780050, Chile"},{"name":"Escuela de Biotecnolog\u00eda, Facultad de Ciencias, Universidad Santo Tomas, Santiago 8320000, Chile"},{"name":"Facultad de Medicina y Ciencia, Universidad San Sebasti\u00e1n, Providencia, Santiago 8320000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1086\/338258","article-title":"Group B Streptococcus colonization in male and nonpregnant female university students: A cross-sectional prevalence study","volume":"34","author":"Bliss","year":"2002","journal-title":"Clin. 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