{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T18:01:06Z","timestamp":1773252066770,"version":"3.50.1"},"reference-count":50,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T00:00:00Z","timestamp":1593043200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["F4605, P29398"],"award-info":[{"award-number":["F4605, P29398"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Respiratory syncytial virus (RSV) infections are a major cause of serious respiratory disease in infants. RSV occurs as two major subgroups A and B, which mainly differ regarding the surface glycoprotein G. The G protein is important for virus attachment and G-specific antibodies can protect against infection. We expressed the surface-exposed part of A2 strain-derived G (A2-G) in baculovirus-infected insect cells and synthesized overlapping peptides spanning complete A2-G. The investigation of the natural IgG response of adult subjects during a period of one year showed that IgG antibodies (i) recognize G significantly stronger than the fusion protein F0, (ii) target mainly non-conformational, sequential peptide epitopes from the exposed conserved region but also buried peptides, and (iii) exhibit a scattered but constant recognition profile during the observation period. The IgG subclass reactivity profile (IgG1 &gt; IgG2 &gt; IgG4 = IgG3) was indicative of a mixed Th1\/Th2 response. Two strongly RSV-neutralizing sera including the 1st WHO standard contained high IgG anti-G levels. G-specific IgG increased strongly in children after wheezing attacks suggesting RSV as trigger factor. Our study shows that RSV G and G-derived peptides are useful for serological diagnosis of RSV-triggered exacerbations of respiratory diseases and underlines the importance of G for development of RSV-neutralizing vaccines.<\/jats:p>","DOI":"10.3390\/vaccines8020337","type":"journal-article","created":{"date-parts":[[2020,6,26]],"date-time":"2020-06-26T05:19:36Z","timestamp":1593148776000},"page":"337","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Features of the Human Antibody Response against the Respiratory Syncytial Virus Surface Glycoprotein G"],"prefix":"10.3390","volume":"8","author":[{"given":"Kristina","family":"Borochova","sequence":"first","affiliation":[{"name":"Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarzyna","family":"Niespodziana","sequence":"additional","affiliation":[{"name":"Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarina","family":"Stenberg Hammar","sequence":"additional","affiliation":[{"name":"Department of Women\u2019s and Children\u2019s Health, Karolinska Institutet, 171 77 Stockholm, Sweden"},{"name":"Astrid Lindgren Children\u2019s Hospital, Karolinska University Hospital, 14186 Stockholm, Sweden"},{"name":"Centre of Allergy Research, Karolinska Institutet, 171 77 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianne","family":"van Hage","sequence":"additional","affiliation":[{"name":"Division of Immunology and Allergy Unit, Department of Medicine, Solna, Karolinska Institutet and Karolinska University Hospital, 171 77 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunilla","family":"Hedlin","sequence":"additional","affiliation":[{"name":"Department of Women\u2019s and Children\u2019s Health, Karolinska Institutet, 171 77 Stockholm, Sweden"},{"name":"Astrid Lindgren Children\u2019s Hospital, Karolinska University Hospital, 14186 Stockholm, Sweden"},{"name":"Centre of Allergy Research, Karolinska Institutet, 171 77 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cilla","family":"S\u00f6derh\u00e4ll","sequence":"additional","affiliation":[{"name":"Department of Women\u2019s and Children\u2019s Health, Karolinska Institutet, 171 77 Stockholm, Sweden"},{"name":"Astrid Lindgren Children\u2019s Hospital, Karolinska University Hospital, 14186 Stockholm, Sweden"},{"name":"Centre of Allergy Research, Karolinska Institutet, 171 77 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarete","family":"Focke-Tejkl","sequence":"additional","affiliation":[{"name":"Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudolf","family":"Valenta","sequence":"additional","affiliation":[{"name":"Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria"},{"name":"NRC Institute of Immunology FMBA of Russia, 115478 Moscow, Russia"},{"name":"Laboratory for Immunopathology, Department of Clinical Immunology and Allergy, Sechenov First Moscow State Medical University, Moscow 119991, Russia"},{"name":"Karl Landsteiner University of Health Sciences, 3500 Krems, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1111\/all.13624","article-title":"Bronchiolitis needs a revisit: Distinguishing between virus entities and their treatments","volume":"74","author":"Jartti","year":"2018","journal-title":"Allergy"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S19","DOI":"10.1097\/01.inf.0000108189.87181.7c","article-title":"Antigenic and genetic variation in human respiratory syncytial virus","volume":"23","author":"Wertz","year":"2004","journal-title":"Pediatr. 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