{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:14:15Z","timestamp":1787030055843,"version":"build-2736575974"},"reference-count":67,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T00:00:00Z","timestamp":1598400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ERGANEO, Ceva Sant\u00e9 Animale, INRAE, Investissement d\u2019Avenir program, Laboratoire d\u2019Excellence Integrative Biology of Emerging Infectious Diseases","award":["ANR-10-LABX-62-IBEID"],"award-info":[{"award-number":["ANR-10-LABX-62-IBEID"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>To identify potential vaccine candidates against Ixodes ricinus and tick-borne pathogen transmission, we have previously sequenced the salivary gland transcriptomes of female ticks infected or not with Bartonella henselae. The hypothesized potential of both IrSPI (I. ricinus serine protease inhibitor) and IrLip1 (I. ricinus lipocalin 1) as protective antigens decreasing tick feeding and\/or the transmission of tick-borne pathogens was based on their presumed involvement in dampening the host immune response to tick feeding. Vaccine endpoints included tick larval and nymphal mortality, feeding, and molting in mice and sheep. Whether the antigens were administered individually or in combination, the vaccination of mice or sheep elicited a potent antigen-specific antibody response. However, and contrary to our expectations, vaccination failed to afford protection against the infestation of mice and sheep by I. ricinus nymphs and larvae, respectively. Rather, vaccination with IrSPI and IrLip1 appeared to enhance tick engorgement and molting and decrease tick mortality. To the best of our knowledge, these observations represent the first report of induction of vaccine-mediated enhancement in relation to anti-tick vaccination.<\/jats:p>","DOI":"10.3390\/vaccines8030475","type":"journal-article","created":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T09:05:37Z","timestamp":1598432737000},"page":"475","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Failed Disruption of Tick Feeding, Viability, and Molting after Immunization of Mice and Sheep with Recombinant Ixodes ricinus Salivary Proteins IrSPI and IrLip1"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3352-1745","authenticated-orcid":false,"given":"Consuelo","family":"Almaz\u00e1n","sequence":"first","affiliation":[{"name":"UMR BIPAR 0956, INRAE, National Veterinary School of Alfort, ANSES, Paris-Est University, 94700 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Fourniol","sequence":"additional","affiliation":[{"name":"UMR BIPAR 0956, INRAE, National Veterinary School of Alfort, ANSES, Paris-Est University, 94700 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Rakotobe","sequence":"additional","affiliation":[{"name":"UMR BIPAR 0956, INRAE, National Veterinary School of Alfort, ANSES, Paris-Est University, 94700 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4472-1016","authenticated-orcid":false,"given":"Ladislav","family":"\u0160imo","sequence":"additional","affiliation":[{"name":"UMR BIPAR 0956, INRAE, National Veterinary School of Alfort, ANSES, Paris-Est University, 94700 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00e9mie","family":"Born\u00e8res","sequence":"additional","affiliation":[{"name":"SEPPIC Paris La D\u00e9fense, 92250 La Garenne Colombes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"Cote","sequence":"additional","affiliation":[{"name":"UMR BIPAR 0956, INRAE, National Veterinary School of Alfort, ANSES, Paris-Est University, 94700 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5520-7346","authenticated-orcid":false,"given":"Sandy","family":"Peltier","sequence":"additional","affiliation":[{"name":"SEPPIC Paris La D\u00e9fense, 92250 La Garenne Colombes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Maye","sequence":"additional","affiliation":[{"name":"SEPPIC Paris La D\u00e9fense, 92250 La Garenne Colombes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Versill\u00e9","sequence":"additional","affiliation":[{"name":"SEPPIC Paris La D\u00e9fense, 92250 La Garenne Colombes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Richardson","sequence":"additional","affiliation":[{"name":"UMR Virologie 1161, INRAE, National Veterinary School of Alfort, ANSES, Paris-Est University, 94700 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6365-6117","authenticated-orcid":false,"given":"Sarah I.","family":"Bonnet","sequence":"additional","affiliation":[{"name":"UMR BIPAR 0956, INRAE, National Veterinary School of Alfort, ANSES, Paris-Est University, 94700 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,26]]},"reference":[{"key":"ref_1","unstructured":"WHO (2020, August 24). Fact Sheet: Vector-Borne Diseases. Available online: http:\/\/www.who.int\/mediacentre\/factsheets\/fs387\/en\/."},{"key":"ref_2","unstructured":"Stuchin, M., Machalaba, C., and Karesh, W. (2016). Vector-borne diseases: Animals and patterns. Forum on Microbial Threats; Board on Global Health; Health and Medicine Division; National Academies of Sciences, Engineering, and Medicine. Global Health Impacts of Vector-Borne Diseases: Workshop Summary, National Academies Press."},{"key":"ref_3","first-page":"6938","article-title":"Overview: Ticks as vectors of pathogens that cause disease in humans and animals","volume":"13","author":"Venzal","year":"2008","journal-title":"Front. Biosci."},{"key":"ref_4","first-page":"251","article-title":"Ixodes ricinus and Its Transmitted Pathogens in Urban and Peri-Urban Areas in Europe: New Hazards and Relevance for Public Health","volume":"2","author":"Rizzoli","year":"2014","journal-title":"Public Health"},{"key":"ref_5","first-page":"74","article-title":"An estimate of Lyme borreliosis incidence in Western Europe","volume":"39","author":"Sykes","year":"2017","journal-title":"J. Public Health"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Vayssier-Taussat, M., Moutailler, S., Michelet, L., Devillers, E., Bonnet, S., Cheval, J., Hebert, C., and Eloit, M. (2013). Next generation sequencing uncovers unexpected bacterial pathogens in ticks in western Europe. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0081439"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Bonnet, S., Michelet, L., Moutailler, S., Cheval, J., Hebert, C., Vayssier-Taussat, M., and Eloit, M. (2014). Identification of Parasitic Communities within European Ticks Using Next-Generation Sequencing. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0002753"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.nmni.2016.02.012","article-title":"Diversity of viruses in Ixodes ricinus, and characterization of a neurotropic strain of Eyach virus","volume":"11","author":"Moutailler","year":"2016","journal-title":"New Microbes New Infect."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Marselle, M., Stadler, J., Korn, H., Irvine, K., and Bonn, A. (2019). Vector-Borne Diseases. Biodiversity and Health in the Face of Climate Change, Springer Nature.","DOI":"10.1007\/978-3-030-02318-8"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Madison-Antenucci, S., Kramer, L.D., Gebhardt, L.L., and Kauffman, E. (2020). Emerging Tick-Borne Diseases. Clin. Microbiol. Rev., 33.","DOI":"10.1128\/CMR.00083-18"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1126\/science.1215735","article-title":"Public health. Monitoring EU emerging infectious disease risk due to climate change","volume":"336","author":"Lindgren","year":"2012","journal-title":"Science"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s10493-012-9615-0","article-title":"Changing distributions of ticks: Causes and consequences","volume":"59","author":"Leger","year":"2013","journal-title":"Exp. Appl. Acarol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.pt.2012.07.003","article-title":"Ticks and tick-borne diseases: A One Health perspective","volume":"28","author":"Chomel","year":"2012","journal-title":"Trends Parasitol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6508","DOI":"10.1016\/j.vaccine.2008.09.044","article-title":"Antigen delivery systems for veterinary vaccine development. Viral-vector based delivery systems","volume":"26","author":"Brun","year":"2008","journal-title":"Vaccine"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"S367","DOI":"10.1017\/S0031182003004657","article-title":"Anti-tick vaccines","volume":"129","author":"Willadsen","year":"2004","journal-title":"J. Parasitol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1586\/14760584.2015.1076339","article-title":"Tick vaccines: Current status and future directions","volume":"14","author":"Contreras","year":"2015","journal-title":"Expert Rev. Vaccines"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1016\/S1286-4579(00)01289-2","article-title":"Issues in tick vaccine development: Identification and characterization of potential candidate vaccine antigens","volume":"2","author":"Mulenga","year":"2000","journal-title":"Microbes Infect."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"137","DOI":"10.2307\/3272354","article-title":"Further observation on acquired immunity to the tick Dermacentor variabilis","volume":"25","author":"Trager","year":"1939","journal-title":"J. Parasitol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"S43","DOI":"10.1017\/S0031182000001487","article-title":"Commercialisation of a recombinant vaccine against Boophilus microplus","volume":"110","author":"Willadsen","year":"1995","journal-title":"J. Parasitol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S1050-3862(99)00018-2","article-title":"Vaccination against ticks (Boophilus spp.): The experience with the Bm86-based vaccine Gavac","volume":"15","author":"Rodriguez","year":"1999","journal-title":"Genet. Anal."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/j.1365-3024.2006.00806.x","article-title":"Exposed and concealed antigens as vaccine targets for controlling ticks and tick-borne diseases","volume":"28","author":"Nuttall","year":"2006","journal-title":"Parasite Immunol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"281","DOI":"10.3389\/fcimb.2017.00281","article-title":"The Essential Role of Tick Salivary Glands and Saliva in Tick Feeding and Pathogen Transmission","volume":"7","author":"Simo","year":"2017","journal-title":"Front. Cell. Infect."},{"key":"ref_23","unstructured":"Boulanger, N. (2018). Tick saliva and its role in pathogen transmission. Skin and Arthropod Vectors, Elsevier."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3284","DOI":"10.1016\/j.vaccine.2007.01.008","article-title":"Protective immunity against Ixodes ricinus induced by a salivary serpin","volume":"25","author":"Prevot","year":"2007","journal-title":"Vaccine"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.ijpara.2007.09.003","article-title":"The impact of gene knock-down and vaccination against salivary metalloproteases on blood feeding and egg laying by Ixodes ricinus","volume":"38","author":"Decrem","year":"2008","journal-title":"Int. J. Parasitol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Labuda, M., Trimnell, A.R., Lickova, M., Kazimirova, M., Davies, G.M., Lissina, O., Hails, R.S., and Nuttall, P.A. (2006). An antivector vaccine protects against a lethal vector-borne pathogen. PLOS Pathog., 2.","DOI":"10.1371\/journal.ppat.0020027"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"977","DOI":"10.3389\/fphys.2019.00977","article-title":"A Vaccinomics Approach for the Identification of Tick Protective Antigens for the Control of Ixodes ricinus and Dermacentor reticulatus Infestations in Companion Animals","volume":"10","author":"Contreras","year":"2019","journal-title":"Front. Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Liu, X.Y., de la Fuente, J., Cote, M., Galindo, R.C., Moutailler, S., Vayssier-Taussat, M., and Bonnet, S.I. (2014). IrSPI, a tick serine protease inhibitor involved in tick feeding and Bartonella henselae infection. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0002993"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.3201\/eid1407.071110","article-title":"Transmission of Bartonella henselae by Ixodes ricinus","volume":"14","author":"Cotte","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"199","DOI":"10.3389\/fcimb.2017.00199","article-title":"Serine Protease Inhibitors in Ticks: An Overview of Their Role in Tick Biology and Tick-Borne Pathogen Transmission","volume":"7","author":"Blisnick","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Blisnick, A.A., Simo, L., Grillon, C., Fasani, F., Brule, S., Le Bonniec, B., Prina, E., Marsot, M., Relmy, A., and Blaise-Boisseau, S. (2019). The Immunomodulatory Effect of IrSPI, a Tick Salivary Gland Serine Protease Inhibitor Involved in Ixodes ricinus Tick Feeding. Vaccines, 7.","DOI":"10.1101\/705921"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/bj3180001","article-title":"The lipocalin protein family: Structure and function","volume":"318","author":"Flower","year":"1996","journal-title":"Biochem. J."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Beaufays, J., Adam, B., Decrem, Y., Prevot, P.P., Santini, S., Brasseur, R., Brossard, M., Lins, L., Vanhamme, L., and Godfroid, E. (2008). Ixodes ricinus tick lipocalins: Identification, cloning, phylogenetic analysis and biochemical characterization. PLoS ONE, 3.","DOI":"10.1371\/journal.pone.0003941"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Beaufays, J., Adam, B., Menten-Dedoyart, C., Fievez, L., Grosjean, A., Decrem, Y., Prevot, P.P., Santini, S., Brasseur, R., and Brossard, M. (2008). Ir-LBP, an ixodes ricinus tick salivary LTB4-binding lipocalin, interferes with host neutrophil function. PLoS ONE, 3.","DOI":"10.1371\/journal.pone.0003987"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"360","DOI":"10.3389\/fcimb.2017.00360","article-title":"Vaccinomics Approach to the Identification of Candidate Protective Antigens for the Control of Tick Vector Infestations and Anaplasma phagocytophilum Infection","volume":"7","author":"Contreras","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.vetpar.2009.09.013","article-title":"The natural history of Anaplasma phagocytophilum","volume":"167","author":"Woldehiwet","year":"2010","journal-title":"Vet. Parasitol."},{"key":"ref_37","first-page":"428","article-title":"Wild animals as reservoirs of Anaplasma phagocytophilum for humans","volume":"70","author":"Dziegiel","year":"2016","journal-title":"Przegl. Epidemiol."},{"key":"ref_38","unstructured":"European Commission (2020, August 24). National Competent Authorities for the Implementation of Directive 2010\/63\/EU on the Protection of Animals Used for Scientific Purposes. Available online: https:\/\/ec.europa.eu\/environment\/chemicals\/lab_animals\/pdf\/guidance\/animal_welfare_bodies\/en.pdf."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1017\/S0031182006001545","article-title":"Transstadial and transovarial persistence of Babesia divergens DNA in Ixodes ricinus ticks fed on infected blood in a new skin-feeding technique","volume":"134","author":"Bonnet","year":"2007","journal-title":"J. Parasitol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Reis, C., Cote, M., Le Rhun, D., Lecuelle, B., Levin, M.L., Vayssier-Taussat, M., and Bonnet, S.I. (2011). Vector competence of the tick Ixodes ricinus for transmission of Bartonella birtlesii. PLoS Negl. Trop. Dis., 5.","DOI":"10.1371\/journal.pntd.0001186"},{"key":"ref_41","first-page":"135","article-title":"Experimental vaccination of sheep and cattle against tick infestation using recombinant 5\u2032-nucleotidase","volume":"32","author":"Hope","year":"2010","journal-title":"Parasites Vectors"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Almaz\u00e1n, C., Fourniol, L., Rouxel, C., Alberdi, P., Gandoin, C., Lagr\u00e9e, A.-C., Boulouis, H.-J., de la Fuente, J., and Bonnet, S.I. (2020). Experimental Ixodes ricinus-Sheep Cycle of Anaplasma phagocytophilum NV2Os Propagated in Tick Cell Cultures. Front. Vet. Sci., 7.","DOI":"10.3389\/fvets.2020.00040"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Boumart, Z., Daouam, S., Belkourati, I., Rafi, L., Tuppurainen, E., Tadlaoui, K.O., and El Harrak, M. (2016). Comparative innocuity and efficacy of live and inactivated sheeppox vaccines. BMC Vet. Res., 12.","DOI":"10.1186\/s12917-016-0754-0"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.ttbdis.2012.01.004","article-title":"Sheep experimentally infected with a human isolate of Anaplasma phagocytophilum serve as a host for infection of Ixodes scapularis ticks","volume":"3","author":"Kocan","year":"2012","journal-title":"Tick Borne Dis."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"32372","DOI":"10.1038\/srep32372","article-title":"Substrate prediction of Ixodes ricinus salivary lipocalins differentially expressed during Borrelia afzelii infection","volume":"6","author":"Valdes","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/j.ijpara.2011.05.003","article-title":"Gene expression evidence for off-target effects caused by RNA interference-mediated gene silencing of Ubiquitin-63E in the cattle tick Rhipicephalus microplus","volume":"41","author":"Kurscheid","year":"2011","journal-title":"Int. J. Parasitol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.vaccine.2016.11.081","article-title":"Bluetongue Disabled Infectious Single Animal (DISA) vaccine: Studies on the optimal route and dose in sheep","volume":"35","author":"Daus","year":"2017","journal-title":"Vaccine"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1046\/j.1365-3024.2001.00412.x","article-title":"Antigenic profile of Ixodes ricinus: Effect of developmental stage, feeding time and the response of different host species","volume":"23","author":"Lawrie","year":"2001","journal-title":"Parasite Immunol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Tirloni, L., Reck, J., Terra, R.M., Martins, J.R., Mulenga, A., Sherman, N.E., Fox, J.W., Yates, J.R., Termignoni, C., and Pinto, A.F. (2014). Proteomic analysis of cattle tick Rhipicephalus (Boophilus) microplus saliva: A comparison between partially and fully engorged females. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0094831"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Kim, T.K., Tirloni, L., Pinto, A.F., Moresco, J., Yates, J.R., da Silva Vaz, I., and Mulenga, A. (2016). Ixodes scapularis Tick Saliva Proteins Sequentially Secreted Every 24 h during Blood Feeding. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004323"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Kim, T.K., Tirloni, L., Pinto, A.F.M., Diedrich, J.K., Moresco, J.J., Yates, J.R., da Silva Vaz, I., and Mulenga, A. (2020). Time-resolved proteomic profile of Amblyomma americanum tick saliva during feeding. PLoS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0007758"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Karim, S., and Ribeiro, J.M. (2015). An Insight into the Sialome of the Lone Star Tick, Amblyomma americanum, with a Glimpse on Its Time Dependent Gene Expression. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0131292"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"517","DOI":"10.3389\/fcimb.2017.00517","article-title":"Tick-Host Range Adaptation: Changes in Protein Profiles in Unfed Adult Ixodes scapularis and Amblyomma americanum Saliva Stimulated to Feed on Different Hosts","volume":"7","author":"Tirloni","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.vaccine.2008.10.087","article-title":"Vaccine-induced enhancement of viral infections","volume":"27","author":"Huisman","year":"2009","journal-title":"Vaccine"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.1073\/pnas.0910344107","article-title":"Antibody-mediated enhancement of community-acquired methicillin-resistant Staphylococcus aureus infection","volume":"107","author":"Yoong","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.pt.2018.11.003","article-title":"Two-Faced Immunity? The Evidence for Antibody Enhancement of Malaria Transmission","volume":"35","author":"Stone","year":"2019","journal-title":"Trends Parasitol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"421","DOI":"10.2174\/1570162X113116660062","article-title":"Antibody-dependent enhancement and the risk of HIV infection","volume":"11","author":"Gorlani","year":"2013","journal-title":"Curr. HIV Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1111\/imr.12367","article-title":"Fc receptors in antibody-dependent enhancement of viral infections","volume":"268","author":"Taylor","year":"2015","journal-title":"Immunol. Rev."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"9640","DOI":"10.1128\/jvi.71.12.9640-9649.1997","article-title":"Enhancement of feline immunodeficiency virus (FIV) infection after DNA vaccination with the FIV envelope","volume":"71","author":"Richardson","year":"1997","journal-title":"J. Virol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2515","DOI":"10.1099\/0022-1317-83-10-2515","article-title":"Lymphoid activation: A confounding factor in AIDS vaccine development?","volume":"83","author":"Richardson","year":"2002","journal-title":"J. Gen. Virol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2248","DOI":"10.1016\/j.vaccine.2011.01.050","article-title":"Control of Rhipicephalus (Boophilus) microplus infestations by the combination of subolesin vaccination and tick autocidal control after subolesin gene knockdown in ticks fed on cattle","volume":"29","author":"Merino","year":"2011","journal-title":"Vaccine"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"5889","DOI":"10.1016\/j.vaccine.2013.09.037","article-title":"Vaccination with proteins involved in tick-pathogen interactions reduces vector infestations and pathogen infection","volume":"31","author":"Merino","year":"2013","journal-title":"Vaccine"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/S0264-410X(97)00208-9","article-title":"Field studies and cost-effectiveness analysis of vaccination with Gavac against the cattle tick Boophilus microplus","volume":"16","author":"Rodriguez","year":"1998","journal-title":"Vaccine"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1016\/j.vaccine.2012.12.073","article-title":"Control of multiple arthropod vector infestations with subolesin\/akirin vaccines","volume":"31","author":"Villar","year":"2013","journal-title":"Vaccine"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1016\/j.vaccine.2017.01.052","article-title":"Control of infestations by Ixodes ricinus tick larvae in rabbits vaccinated with aquaporin recombinant antigens","volume":"35","author":"Contreras","year":"2017","journal-title":"Vaccine"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"4329","DOI":"10.1016\/j.vaccine.2005.03.041","article-title":"A cross-reactive tick cement antigen is a candidate broad-spectrum tick vaccine","volume":"23","author":"Trimnell","year":"2005","journal-title":"Vaccine"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1615\/CritRevImmunol.v21.i1-3.50","article-title":"Induction and direction of immune responses by vaccine adjuvants","volume":"21","author":"Schijns","year":"2001","journal-title":"Crit. 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