{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T10:04:43Z","timestamp":1768644283399,"version":"3.49.0"},"reference-count":43,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,10,12]],"date-time":"2020-10-12T00:00:00Z","timestamp":1602460800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Background: Despite the availability of an effective vaccine since the 1970s, rubella disease and, importantly, congenital rubella syndrome (CRS) remain a public health concern. The aim of this study was to analyze the rubella seroprevalence in the children population of the province of Florence and compare the obtained results to a previous survey conducted in 2005\u20132006. Methods: A qualitative measurement of anti-rubella antibodies was performed on 165 sera using the enzyme-linked immunosorbent Assay (ELISA). The anamnestic and vaccination status was also collected. Results: Our study highlighted a very high rubella seroprevalence (85\u2013100%) in our enrolled population. In the vaccinated group (153\/165), 98.7% of them were positive to rubella antibodies. Conclusions: Our study showed the highest seroprevalence rate reached in the province of Florence for rubella in the last 15 years, thanks to the several successful vaccination campaigns promoted in the Tuscany region. Our findings in pediatric and adolescent subjects are a key factor in preventing CRS in adult life, specifically in childbearing women. Thus, the set goal will be to keep the awareness about the vaccination for this preventable disease high.<\/jats:p>","DOI":"10.3390\/vaccines8040599","type":"journal-article","created":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T21:48:38Z","timestamp":1602625718000},"page":"599","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Rubella Seroprevalence Boost in the Pediatric and Adolescent Population of Florence (Italy) as a Preventive Strategy for Congenital Rubella Syndrome (CRS)"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6718-1932","authenticated-orcid":false,"given":"Beatrice","family":"Zanella","sequence":"first","affiliation":[{"name":"Department of Health Sciences, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9695-7549","authenticated-orcid":false,"given":"Sara","family":"Boccalini","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6116-8777","authenticated-orcid":false,"given":"Benedetta","family":"Bonito","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2742-0297","authenticated-orcid":false,"given":"Marco","family":"Del Riccio","sequence":"additional","affiliation":[{"name":"Medical Specialization School of Hygiene and Preventive Medicine, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Manzi","sequence":"additional","affiliation":[{"name":"Medical Specialization School of Hygiene and Preventive Medicine, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilia","family":"Tiscione","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Bonanni","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Working Group DHS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Working Group AOUMeyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Working Group AUSLTC","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6013-8779","authenticated-orcid":false,"given":"Angela","family":"Bechini","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,12]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"R\u00f6theln (epidemic roseola-German measles-hybrid measles, etc.)","volume":"1","author":"Smith","year":"1875","journal-title":"Arch. Dermatol."},{"key":"ref_2","first-page":"35","article-title":"Congenital cataract following German measles in the mother","volume":"3","author":"Gregg","year":"1941","journal-title":"Trans. Ophthalmol. Soc. Aust."},{"key":"ref_3","first-page":"1","article-title":"Rubella during pregnancy: A follow-up study of children born after an epidemic of rubella in Sweden, 1951, with additional investigations on prophylaxis and treatment of maternal rubella","volume":"133","author":"Lundstrom","year":"1962","journal-title":"Acta Paediatr. Scand."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1001\/jama.1957.02980240033009","article-title":"Frequency of defects in infants whose mothers had rubella during pregnancy","volume":"165","author":"Greenberg","year":"1957","journal-title":"JAMA"},{"key":"ref_5","unstructured":"Manson, M., Logan, W.P.D., and Loy, R. (1960). Rubella and Other Virus Infections during Pregnancy (Report on Public Health and Mechanical Subjects, No. 101), HM Stationery Office."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/S0022-3476(65)80240-2","article-title":"Some recently recognized manifestations of the rubella syndrome","volume":"67","author":"Plotkin","year":"1965","journal-title":"J. Pediatr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1001\/archpedi.1969.02100040174003","article-title":"Experimental live attenuated rubella virus vaccine: clinical evaluation of Cendehill strain","volume":"118","author":"Prinzie","year":"1969","journal-title":"Am. J. Dis. Child."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1001\/archpedi.1969.02100040157001","article-title":"Attenuated rubella viruses: Laboratory and clinical characteristics","volume":"118","author":"Meyer","year":"1969","journal-title":"Am. J. Dis. Child."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Public Health Service Advisory Committee on Immunisation Practices (1969). Rubella virus vaccine recommendation on immunisation practice: A pre-licensing statement. Ann. Intern. Med., 70, 1239\u20131241.","DOI":"10.7326\/0003-4819-70-6-1239"},{"key":"ref_10","unstructured":"WHO (1983). The Immunological Basis for Immunization Series - Module 11: Rubella. Immun. Vaccines Biol., 11, 1\u201324."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1016\/B978-0-323-35761-6.00052-3","article-title":"Rubella vaccines","volume":"Volume 53","author":"Reef","year":"2018","journal-title":"Plotkin\u2019s Vaccines"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1136\/ard.42.1.2","article-title":"Chronic arthritis associated with the presence of intrasynovial rubella virus","volume":"42","author":"Grahame","year":"1983","journal-title":"Ann. Rheum. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1001\/archinte.1966.03870100101018","article-title":"Thrombocytopenic purpura following rubella infection in children and adults","volume":"117","author":"Morse","year":"1966","journal-title":"Arch. Intern. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1001\/jama.1965.03080210019005","article-title":"Acute encephalopathy (encephalitis) complicating rubella: Reports of cases with virologic studies, cortisol-production determinations and observations at autopsy","volume":"192","author":"Sherman","year":"1965","journal-title":"JAMA"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2297","DOI":"10.1016\/S0140-6736(14)60539-0","article-title":"Rubella","volume":"385","author":"Lambert","year":"2015","journal-title":"Lancet"},{"key":"ref_16","unstructured":"World Health Organization (2019). Eighth Meeting of the European Regional Verification Commission for Measles and Rubella Elimination (Rvc), World Health Organization."},{"key":"ref_17","unstructured":"World Health Organization (2014). Eliminating Measles and Rubella, World Health Organization."},{"key":"ref_18","unstructured":"WHO Regional Office for Europe (2014). Eliminating Measles and Rubella. Framework for the Verification Process in the WHO European Region, World Health Organization."},{"key":"ref_19","first-page":"1","article-title":"The end of measles and congenital rubella: An achievable dream?","volume":"29","author":"Adamo","year":"2017","journal-title":"Ann. Ig."},{"key":"ref_20","unstructured":"(2020, September 02). Ministero della Salute Piano nazionale per l\u2019Eliminazione del Morbillo e della Rosolia Congenita 2010\u20132015, Available online: http:\/\/www.salute.gov.it\/imgs\/C_17_pubblicazioni_1519_allegato.pdf."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"649","DOI":"10.4161\/hv.23261","article-title":"Progress in the elimination of measles and congenital rubella in Central Italy","volume":"9","author":"Bechini","year":"2013","journal-title":"Hum. Vaccines Immunother."},{"key":"ref_22","unstructured":"(2020, July 20). Istituto Superiore di Sanit\u00e0 Morbillo e Rosolia News. Available online: https:\/\/www.epicentro.iss.it\/morbillo\/bollettino\/RM_News_2019_59.pdf."},{"key":"ref_23","unstructured":"ARS Toscana (2020, July 20). La Sorveglianza Epidemiologica delle mMlattie Infettive in Toscana.Rapporto Novembre 2019. Available online: https:\/\/www.ars.toscana.it\/images\/pubblicazioni\/Rapporti\/2019\/Rapporto_malattie_infettive.pdf."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Zanella, B., Boccalini, S., Bonito, B., Del Riccio, M., Tiscione, E., Bonanni, P., Dhs, W.G., Aoumeyer, W.G., Ausltc, W.G., and Bechini, A. (2020). Increasing Measles Seroprevalence in a Sample of Pediatric and Adolescent Population of Tuscany (Italy): A Vaccination Campaign Success. Vaccines, 8.","DOI":"10.3390\/vaccines8030512"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Zanella, B., Bechini, A., Boccalini, S., Sartor, G., Tiscione, E., and Bonanni, P. (2020). Hepatitis B Seroprevalence in the Pediatric and Adolescent Population of Florence (Italy): An Update 27 Years after the Implementation of Universal Vaccination. Vaccines, 8.","DOI":"10.3390\/vaccines8020156"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1093\/eurpub\/ckq134","article-title":"Progress towards measles and rubella elimination in Tuscany, Italy: The role of population seroepidemiological profile","volume":"22","author":"Bechini","year":"2010","journal-title":"Eur. J. Public Health"},{"key":"ref_27","unstructured":"GeoDemo Istat (2020, July 20). Popolazione Residente al 1\u00b0 Gennaio 2017 nella Provincia di Firenze. Available online: http:\/\/demo.istat.it\/pop2017\/index.html."},{"key":"ref_28","unstructured":"Epicentro (2020, July 20). La sorveglianza Passi.I Dati per l\u2019Italia-Vaccinazione Antirosolia. Available online: https:\/\/www.epicentro.iss.it\/passi\/dati\/VaccinazioneAntirosolia."},{"key":"ref_29","unstructured":"Istituto Nazionale di Statistica (2019). Natalit\u00e0 e Fecondit\u00e0 della Popolazione Residente | Anno 2018 Natalit\u00e0, Istituto Nazionale di Statistica."},{"key":"ref_30","unstructured":"World Health Organization (2010). European Regional Strategic Plan 2005\u20132010. Eliminating Measles and Rubella and Preventing Congenital Rubella, World Health Organization."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4860","DOI":"10.1016\/j.vaccine.2013.11.006","article-title":"Addressing the anti-vaccination movement and the role of HCWs","volume":"32","author":"Tafuri","year":"2014","journal-title":"Vaccine"},{"key":"ref_32","unstructured":"Ministero della Salute (2020, July 23). Decreto Legge 7 giugno 2017, Available online: http:\/\/www.trovanorme.salute.gov.it\/norme\/dettaglioAtto?id=59548."},{"key":"ref_33","unstructured":"WHO (2020, September 02). Vaccine-Preventable Diseases: Monitoring system. 2019 Global Summary Italy. Available online: https:\/\/apps.who.int\/immunization_monitoring\/globalsummary\/countries?countrycriteria%5Bcountry%5D%5B%5D=ITA."},{"key":"ref_34","unstructured":"WHO (2020, September 02). Vaccine-Preventable Diseases: Monitoring System. 2019 Global Summary Senegal. Available online: https:\/\/apps.who.int\/immunization_monitoring\/globalsummary\/countries?countrycriteria%5Bcountry%5D%5B%5D=SEN#."},{"key":"ref_35","unstructured":"WHO (2020, September 02). Vaccine-Preventable Diseases: Monitoring System. 2019 Global Summary Morocco. Available online: https:\/\/apps.who.int\/immunization_monitoring\/globalsummary\/countries?countrycriteria%5Bcountry%5D%5B%5D=MAR."},{"key":"ref_36","unstructured":"WHO (2020, September 02). Vaccine-Preventable Diseases: Monitoring System. 2019 Global Summary Romania. Available online: https:\/\/apps.who.int\/immunization_monitoring\/globalsummary\/countries?countrycriteria%5Bcountry%5D%5B%5D=ROU."},{"key":"ref_37","unstructured":"WHO (2020, September 02). Vaccine-Preventable Diseases: Monitoring System. 2019 Global Summary Philippines. Available online: https:\/\/apps.who.int\/immunization_monitoring\/globalsummary\/countries?countrycriteria%5Bcountry%5D%5B%5D=PHL."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Bechini, A., Boccalini, S., Alimenti, C.M., Bonanni, P., Galli, L., and Chiappini, E. (2020). Immunization status against measles, mumps, rubella and varicella in a large population of internationally adopted children referred to Meyer children\u2019s university hospital from 2009 to 2018. Vaccines, 8.","DOI":"10.3390\/vaccines8010051"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1007\/s00431-017-2935-7","article-title":"Underreporting of congenital rubella in Italy, 2010\u20132014","volume":"176","author":"Giambi","year":"2017","journal-title":"Eur. J. Pediatr."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1017\/S0950268899004574","article-title":"The seroepidemiology of rubella in Western Europe","volume":"125","author":"Pebody","year":"2000","journal-title":"Epidemiol. Infect."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2634","DOI":"10.4161\/hv.26010","article-title":"An overview of current and potential use of information and communication technologies for immunization promotion among adolescents","volume":"9","author":"Amicizia","year":"2013","journal-title":"Hum. Vaccines Immunother."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3089","DOI":"10.1016\/j.vaccine.2017.04.033","article-title":"The impacts of email reminder\/recall on adolescent influenza vaccination","volume":"35","author":"Dombkowski","year":"2017","journal-title":"Vaccine"},{"key":"ref_43","unstructured":"Ministero della Salute (2020, September 02). Vaccinazioni dell\u2019et\u00e0 Pediatrica e dell\u2019Adolescenza\u2013Coperture Vaccinali, Available online: http:\/\/www.salute.gov.it\/portale\/documentazione\/p6_2_8_3_1.jsp?lingua=italiano&id=20."}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/8\/4\/599\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:19:55Z","timestamp":1760177995000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/8\/4\/599"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,12]]},"references-count":43,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["vaccines8040599"],"URL":"https:\/\/doi.org\/10.3390\/vaccines8040599","relation":{},"ISSN":["2076-393X"],"issn-type":[{"value":"2076-393X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,12]]}}}