{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T05:40:00Z","timestamp":1768801200616,"version":"3.49.0"},"reference-count":41,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T00:00:00Z","timestamp":1607299200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institute of Pharmaceutical Malaysia (IPHARM), Ministry of Science and Technology (MOSTI)","award":["9358000"],"award-info":[{"award-number":["9358000"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Enterovirus 71 (EV71) is the major causative agent in hand, foot, and mouth disease (HFMD), and it mainly infects children worldwide. Despite the risk, there is no effective vaccine available for this disease. Hence, a recombinant protein construct of truncated nucleocapsid protein viral protein 1 (NPt-VP1198\u2013297), which is capable of inducing neutralizing antibody against EV71, was evaluated in a mouse model. Truncated nucleocapsid protein Newcastle disease virus that was used as immunological carrier fused to VP1 of EV71 as antigen. The recombinant plasmid carrying corresponding genes was constructed by recombinant DNA technology and the corresponding protein was produced in Escherichia coli expression system. The recombinant NPt-VP1198\u2013297 protein had elicited neutralizing antibodies against EV71 with the titer of 1:16, and this result is higher than the titer that is elicited by VP1 protein alone (1:8). It was shown that NPt containing immunogenic epitope(s) of VP1 was capable of inducing a greater functional immune response when compared to full-length VP1 protein alone. It was capable to carry larger polypeptide compared to full-length NP protein. The current study also proved that NPt-VP1198\u2013297 protein can be abundantly produced in recombinant protein form by E. coli expression system. The findings from this study support the importance of neutralizing antibodies in EV71 infection and highlight the potential of the recombinant NPt-VP1198\u2013297 protein as EV71 vaccine.<\/jats:p>","DOI":"10.3390\/vaccines8040742","type":"journal-article","created":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T12:24:39Z","timestamp":1607343879000},"page":"742","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Recombinant Enterovirus 71 Viral Protein 1 Fused to a Truncated Newcastle Disease Virus NP (NPt) Carrier Protein"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3384-1397","authenticated-orcid":false,"given":"Suhaili","family":"Mustafa","sequence":"first","affiliation":[{"name":"Department of Animal Science and Fishery, Universiti Putra Malaysia, Bintulu Sarawak Campus, Bintulu 97008, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noraini","family":"Abd-Aziz","sequence":"additional","affiliation":[{"name":"Centre for Virus and Vaccine Research (CVVR), School of Science and Technology, Sunway University, Subang Jaya 47500, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wuan-Ting","family":"Saw","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Universiti Putra Malaysia, Serdang 43400, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sien-Yei","family":"Liew","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Universiti Putra Malaysia, Serdang 43400, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khatijah","family":"Yusoff","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Universiti Putra Malaysia, Serdang 43400, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norazizah","family":"Shafee","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Universiti Putra Malaysia, Serdang 43400, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/S1473-3099(13)70342-6","article-title":"Hand, Foot, and Mouth Disease in China, 2008\u20132012: An Epidemiological Study","volume":"14","author":"Xing","year":"2014","journal-title":"Lancet Infect. 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