{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T18:30:45Z","timestamp":1780943445993,"version":"3.54.1"},"reference-count":23,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,3,24]],"date-time":"2021-03-24T00:00:00Z","timestamp":1616544000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>Pre-exposure rabies prophylaxis (PrEP) is recommended for people at frequent or increased risk of professional exposure to lyssavirus (including rabies virus). PrEP provides protection against unrecognized exposure. After the primary vaccination, one\u2019s immune response against rabies may decline over time. We aimed to evaluate the immune response to rabies in individuals immunized for occupational reasons before and after a booster dose of the rabies vaccine. With this aim, we retrospectively documented factors associated with an inadequate response in individuals vaccinated for occupational purposes. Our findings analyzed data from 498 vaccinated individuals and found that 17.2% of participants had an inadequate antibody titration documented after their primary vaccination without the booster, while inadequate response after an additional booster of the vaccine was evidenced in 0.5% of tested participants. This study showed that a single booster dose of vaccine after PrEP conferred a high and long-term immune response in nearly all individuals except for rare, low responders. A systematic rabies booster after primary vaccination may result in alleviating the monitoring strategy of post-PrEP antibody titers among exposed professionals.<\/jats:p>","DOI":"10.3390\/vaccines9040309","type":"journal-article","created":{"date-parts":[[2021,3,24]],"date-time":"2021-03-24T21:36:51Z","timestamp":1616621811000},"page":"309","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Systematic Booster after Rabies Pre-Exposure Prophylaxis to Alleviate Rabies Antibody Monitoring in Individuals at Risk of Occupational Exposure"],"prefix":"10.3390","volume":"9","author":[{"given":"Perrine","family":"Parize","sequence":"first","affiliation":[{"name":"Institut Pasteur, Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Centre for Reference and Research on Rabies, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5804-0123","authenticated-orcid":false,"given":"J\u00e9r\u00e9mie","family":"Somm\u00e9","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Occupational Health Department, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Schaeffer","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Emerging Diseases Epidemiology Unit, Centre for Global Health Research and Education, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Ribadeau-Dumas","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Centre for Reference and Research on Rabies, 75015 Paris, 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Guern","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Laboratory of the Medical Center, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Laurence","family":"Mousel","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Occupational Health Department, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4401-3706","authenticated-orcid":false,"given":"Laurent","family":"Dacheux","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Centre for Reference and Research on Rabies, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul-Henri","family":"Consigny","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Centre M\u00e9dical, Centre d\u2019Infectiologie Necker-Pasteur, 75015 Paris, 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(2015). Estimating the global burden of endemic canine rabies. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003786"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"17091","DOI":"10.1038\/nrdp.2017.91","article-title":"Rabies","volume":"3","author":"Fooks","year":"2017","journal-title":"Nat. Rev. Dis. Primer"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"World Health Organization (2018). Rabies Vaccines: WHO Position Paper, April 2018\u2014Recommendations. Vaccine, 36, 5500\u20135503.","DOI":"10.1016\/j.vaccine.2018.06.061"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/S1474-4422(13)70038-3","article-title":"Human rabies: Neuropath-ogenesis, diagnosis, and management","volume":"12","author":"Hemachudha","year":"2013","journal-title":"Lancet Neurol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1932","DOI":"10.1093\/cid\/ciy420","article-title":"Rabies Antibody Response after Booster Im-munization: A Systematic Review and Meta-analysis","volume":"67","author":"Langedijk","year":"2018","journal-title":"Clin Infect. Dis."},{"key":"ref_6","unstructured":"World Health Organization (2018). WHO Expert Consultation on Rabies: Third Report, World Health Organization."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"210C","DOI":"10.2471\/BLT.16.173039","article-title":"Pre-exposure rabies prophylaxis: A systematic review","volume":"95","author":"Kessels","year":"2016","journal-title":"Bull. World Health Organ."},{"key":"ref_8","unstructured":"World Health Organization (2005). WHO Expert Consultation on Rabies, World Health Organization."},{"key":"ref_9","unstructured":"World Health Organization (2013). WHO Expert Consultation on Rabies. Second Report, World Health Organization."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e511","DOI":"10.1016\/j.ijid.2009.08.008","article-title":"Serologic response to rabies pre-exposure vaccination in persons with potential occupational exposure in Singapore","volume":"14","author":"Lim","year":"2010","journal-title":"Int. J. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5959","DOI":"10.1016\/j.vaccine.2016.09.058","article-title":"Rabies pre-exposure prophylaxis elicits long-lasting immunity in humans","volume":"34","author":"Mansfield","year":"2016","journal-title":"Vaccine"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1089\/vbz.2019.2526","article-title":"Antibody Response Following Pre-Exposure Immunization Against Rabies in High-Risk Professionals","volume":"20","author":"Dougas","year":"2019","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2244","DOI":"10.1016\/j.vaccine.2006.12.012","article-title":"Multicenter comparative study of a new ELISA, PLATELIA\u2122 RABIES II, for the detection and titration of anti-rabies glycoprotein antibodies and comparison with the rapid fluorescent focus inhibition test (RFFIT) on human samples from vaccinated and non-vaccinated people","volume":"25","author":"Feyssaguet","year":"2007","journal-title":"Vaccine"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1016\/j.vaccine.2013.12.019","article-title":"Incidence and variables associated with inadequate antibody titers after pre-exposure rabies vaccination among veterinary medical students","volume":"32","author":"Banga","year":"2014","journal-title":"Vaccine"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Medeiros, R., Jusot, V., Houillon, G., Rasuli, A., Martorelli, L., Kataoka, A.P., Ben Mechlia, M., Le Guern, A.-S., Rodrigues, L., and Assef, R. (2016). Persistence of Rabies Virus-Neutralizing Antibodies after Vaccination of Rural Population following Vampire Bat Rabies Outbreak in Brazil. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004920"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3551","DOI":"10.1016\/j.vaccine.2008.04.054","article-title":"Sexual dimorphism of humoral immunity with human vaccines","volume":"26","author":"Cook","year":"2008","journal-title":"Vaccine"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/S1473-3099(10)70049-9","article-title":"The Xs and Y of immune responses to viral vaccines","volume":"10","author":"Klein","year":"2010","journal-title":"Lancet Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/S1567-5769(01)00044-3","article-title":"Sex hormones as immunomodulators in health and disease","volume":"1","author":"Verthelyi","year":"2001","journal-title":"Int. Immunopharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1111\/j.1708-8305.2011.00540.x","article-title":"The Immunogenicity of a Modified Intradermal Pre-exposure Rabies Vaccination Schedule\u2014A Case Series of 420 Travelers","volume":"18","author":"Mills","year":"2011","journal-title":"J. Travel Med."},{"key":"ref_20","first-page":"27","article-title":"Intradermal rabies pre-exposure vaccination schedules in older travellers: Comparison of immunogenicity post-primary course and post-booster","volume":"27","author":"Ramsey","year":"2020","journal-title":"J. Travel Med."},{"key":"ref_21","unstructured":"ACIP (2020, June 19). Timing and Spacing Guidelines for Immunization. Recommendations. CDC, Available online: https:\/\/www.cdc.gov\/vaccines\/hcp\/acip-recs\/general-recs\/timing.html."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"68","DOI":"10.7774\/cevr.2015.4.1.68","article-title":"Cellular immune response following pre-exposure and postexposure rabies vaccination by intradermal and intramuscular routes","volume":"4","author":"Venkataswamy","year":"2015","journal-title":"Clin. Exp. Vaccine Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"6060","DOI":"10.1016\/j.vaccine.2019.08.043","article-title":"Rabies virus glycoprotein serology ELISA for measurement of neu-tralizing antibodies in sera of vaccinated human subjects","volume":"37","author":"Zhao","year":"2019","journal-title":"Vaccine"}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/9\/4\/309\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:40:22Z","timestamp":1760161222000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/9\/4\/309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,24]]},"references-count":23,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,4]]}},"alternative-id":["vaccines9040309"],"URL":"https:\/\/doi.org\/10.3390\/vaccines9040309","relation":{},"ISSN":["2076-393X"],"issn-type":[{"value":"2076-393X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,24]]}}}