{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:26:28Z","timestamp":1790504788424,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,7]],"date-time":"2021-06-07T00:00:00Z","timestamp":1623024000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research on Key Technology of SARS-CoV-2 and Influenza A\u3001 B Virus 328 Antigen Rapid Quantitative Combined Test Strip","award":["LGF21H200007"],"award-info":[{"award-number":["LGF21H200007"]}]},{"name":"Monitor Technology Platform of Infectious Diseases of 325 The State Major Science and Technology Special Projects during the 13th Five-year Plan of China","award":["2017ZX10103008-002"],"award-info":[{"award-number":["2017ZX10103008-002"]}]},{"name":"General Research Project of Zhejiang Shuren University in 2019","award":["2019A11001"],"award-info":[{"award-number":["2019A11001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccines"],"abstract":"<jats:p>At present, there are no vaccines available for hand, foot, and mouth disease, which is caused by Coxsackie virus A16 (CVA16) infection. In the present study, we isolated epidemic strains of CVA16 and optimized the production of the virus in Vero cells. The system comprised growing the infected cells on polymer fiber paper carriers in a serum-free medium containing 0.5% (w\/v) lactalbumin hydrolysate a mini bioreactor. Disposable Bioflo310 and AmProtein Current perfusion bioreactors were used to monitor virus infection and Vero cell culture. The total number of cells increased from 1.5 \u00d7 109 to 3.0 \u00d7 1010. In our optimized culture process, the virus titer reached 7.8 \u00d7 107 TCID50\/mL at three days after infection. The inactivated CVA16 prepared from our optimized culture procedure elicited a slightly higher neutralizing antibody titer compared with that derived from routine culture procedures. These results will promote the large-scale production of inactivated CVA16 vaccines using nonwoven polymer fiber paper cell cultures.<\/jats:p>","DOI":"10.3390\/vaccines9060613","type":"journal-article","created":{"date-parts":[[2021,6,7]],"date-time":"2021-06-07T22:23:00Z","timestamp":1623104580000},"page":"613","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Optimization of Vero Cells Grown on a Polymer Fiber Carrier in a Disposable Bioreactor for Inactivated Coxsackievirus A16 Vaccine Development"],"prefix":"10.3390","volume":"9","author":[{"given":"Keda","family":"Chen","sequence":"first","affiliation":[{"name":"Shulan International Medical College, Zhejiang Shuren University, Hangzhou 310015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5514-842X","authenticated-orcid":false,"given":"Chaonan","family":"Li","sequence":"additional","affiliation":[{"name":"Shulan International Medical College, Zhejiang Shuren University, Hangzhou 310015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Shulan International Medical College, Zhejiang Shuren University, Hangzhou 310015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenwei","family":"Shen","sequence":"additional","affiliation":[{"name":"Shulan International Medical College, Zhejiang Shuren University, Hangzhou 310015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yikai","family":"Guo","sequence":"additional","affiliation":[{"name":"Shulan International Medical College, Zhejiang Shuren University, Hangzhou 310015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Li","sequence":"additional","affiliation":[{"name":"Shulan International Medical College, Zhejiang Shuren University, Hangzhou 310015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Virus Inspection, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.7774\/cevr.2017.6.1.4","article-title":"Enterovirus 71 infection and vaccines","volume":"6","author":"Yi","year":"2017","journal-title":"Clin. Exp. Vaccine Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.envint.2016.11.021","article-title":"The exposure-response relationship between temperature and childhood hand, foot and mouth disease: A multicity study from mainland China","volume":"100","author":"Xiao","year":"2017","journal-title":"Environ. Int."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Xiao, X., Liao, Q., Kenward, M.G., Zheng, Y., Huang, J., Yin, F., Yu, H., and Li, X. (2016). Comparisons between mild and severe cases of hand, foot and mouth disease in temporal trends: A comparative time series study from mainland China. BMC Public Health, 16.","DOI":"10.1186\/s12889-016-3762-x"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"6400","DOI":"10.3390\/v7122947","article-title":"Epidemiological Research on Hand, Foot, and Mouth Disease in Mainland China","volume":"7","author":"Zhuang","year":"2015","journal-title":"Viruses"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1099\/jmm.0.079699-0","article-title":"Molecular epidemiology of enterovirus 71 strains isolated from children in Yamagata, Japan, between 1990 and 2013","volume":"63","author":"Mizuta","year":"2014","journal-title":"J. Med. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Yin, X.G., Yi, H.X., Shu, J., Wang, X.J., Wu, X.J., and Yu, L.H. (2014). Clinical and epidemiological characteristics of adult hand, foot, and mouth disease in northern Zhejiang, China, May 2008\u2013November 2013. BMC Infect. Dis., 14.","DOI":"10.1186\/1471-2334-14-251"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.antiviral.2014.02.015","article-title":"A murine model of coxsackievirus A16 infection for anti-viral evaluation","volume":"105","author":"Liu","year":"2014","journal-title":"Antivir. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1016\/j.vaccine.2013.03.007","article-title":"Active immunization with a Coxsackievirus A16 experimental inactivated vaccine induces neutralizing antibodies and protects mice against lethal infection","volume":"31","author":"Cai","year":"2013","journal-title":"Vaccine"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"106","DOI":"10.47102\/annals-acadmedsg.V38N2p106","article-title":"Epidemiology and control of hand, foot and mouth disease in Singapore, 2001\u20132007","volume":"38","author":"Ang","year":"2009","journal-title":"Ann. Acad. Med. Singap."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2406","DOI":"10.1016\/j.vaccine.2014.03.012","article-title":"A combination vaccine comprising of inactivated enterovirus 71 and coxsackievirus A16 elicits balanced protective immunity against both viruses","volume":"32","author":"Cai","year":"2014","journal-title":"Vaccine"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.jviromet.2011.01.016","article-title":"Detection, characterization and quantitation of coxsackievirus A16 using polyclonal antibodies against recombinant capsid subunit proteins","volume":"173","author":"Liu","year":"2011","journal-title":"J. Virol. Methods"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"229","DOI":"10.7883\/yoken.63.229","article-title":"Clinical and molecular characterization of hand-foot-and-mouth disease in Thailand, 2008\u20132009","volume":"63","author":"Chatproedprai","year":"2010","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.3201\/eid1311.070632","article-title":"Epidemiologic and virologic investigation of hand, foot, and mouth disease, southern Vietnam, 2005","volume":"13","author":"Tu","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3835","DOI":"10.1128\/JCM.43.8.3835-3839.2005","article-title":"Genetic characteristics of human enterovirus 71 and coxsackievirus A16 circulating from 1999 to 2004 in Shenzhen, People\u2019s Republic of China","volume":"43","author":"Li","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1128\/JCM.00718-06","article-title":"Genetic diversity of coxsackievirus A16 associated with hand, foot, and mouth disease epidemics in Japan from 1983 to 2003","volume":"45","author":"Hosoya","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.jcv.2009.02.002","article-title":"An outbreak of hand, foot, and mouth disease associated with subgenotype C4 of human enterovirus 71 in Shandong, China","volume":"44","author":"Zhang","year":"2009","journal-title":"J. Clin. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1111\/j.1574-6976.2002.tb00601.x","article-title":"An overview of the evolution of enterovirus 71 and its clinical and public health significance","volume":"26","author":"McMinn","year":"2002","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1097\/00006454-199912000-00013","article-title":"Comparison of enterovirus 71 and coxsackie-virus A16 clinical illnesses during the Taiwan enterovirus epidemic, 1998","volume":"18","author":"Chang","year":"1999","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"254","DOI":"10.7883\/yoken.JJID.2009.254","article-title":"Genetic changes of coxsackievirus A16 and enterovirus 71 isolated from hand, foot, and mouth disease patients in Toyama, Japan between 1981 and 2007","volume":"62","author":"Iwai","year":"2009","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1016\/S1473-3099(10)70194-8","article-title":"Virology, epidemiology, pathogenesis, and control of enterovirus 71","volume":"10","author":"Solomon","year":"2010","journal-title":"Lancet Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1097\/01.inf.0000115950.63906.78","article-title":"Fatal coxsackievirus A16 infection","volume":"23","author":"Wang","year":"2004","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1056\/NEJM196305092681904","article-title":"Fatal infection in an infant associated with Coxsackie virus group A, type 16","volume":"268","author":"Wright","year":"1963","journal-title":"N. Engl. J. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1743-422X-9-8","article-title":"Distribution of enteroviruses in hospitalized children with hand, foot and mouth disease and relationship between pathogens and nervous system complications","volume":"9","author":"Xu","year":"2012","journal-title":"Virol. J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3858","DOI":"10.1016\/j.vaccine.2004.05.037","article-title":"Optimization of microcarrier cell culture process for the inactivated enterovirus type 71 vaccine development","volume":"22","author":"Wu","year":"2004","journal-title":"Vaccine"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.vaccine.2006.06.083","article-title":"High immunogenic enterovirus 71 strain and its production using serum-free microcarrier Vero cell culture","volume":"25","author":"Liu","year":"2007","journal-title":"Vaccine"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1007\/s00253-012-4375-7","article-title":"Production of influenza H1N1 vaccine from MDCK cells using a novel disposable packed-bed bioreactor","volume":"97","author":"Sun","year":"2013","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_27","first-page":"46","article-title":"A Single-Use, Scalable Perfusion Bioreactor System","volume":"7","author":"Li","year":"2009","journal-title":"Bioprocess. Int."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6469","DOI":"10.1007\/s00253-018-9091-5","article-title":"Process optimization for the rapid production of adenoviral vectors for clinical trials in a disposable bioreactor system","volume":"102","author":"Chen","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1021\/bp000110+","article-title":"Application of a serum-free medium for the growth of Vero cells and the production of reovirus","volume":"16","author":"Butler","year":"2000","journal-title":"Biotechnol. Prog."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1390","DOI":"10.1016\/0264-410X(93)90167-V","article-title":"Risk factors for systemic hypersensitivity reactions after booster vaccinations with human diploid cell rabies vaccine: A nationwide prospective study","volume":"11","author":"Fishbein","year":"1993","journal-title":"Vaccine"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Hu, A.Y., Tseng, Y.F., Weng, T.C., Liao, C.C., Wu, J., Chou, A.H., Chao, H.J., Gu, A., Chen, J., and Lin, S.C. (2011). Production of inactivated influenza H5N1 vaccines from MDCK cells in serum-free medium. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0014578"},{"key":"ref_32","first-page":"493","article-title":"A simple method of estimating 50 percent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Hyg."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Chong, P., Guo, M.S., Lin, F.H., Hsiao, K.N., Weng, S.Y., Chou, A.H., Wang, J.R., Hsieh, S.Y., Su, I.J., and Liu, C.C. (2012). Immunological and biochemical characterization of coxsackie virus A16 viral particles. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0049973"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"581","DOI":"10.3201\/1504.081550","article-title":"Enterovirus 71 maternal antibodies in infants, Taiwan","volume":"15","author":"Luo","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Chou, A.H., Liu, C.C., Chang, C.P., Guo, M.S., Hsieh, S.Y., Yang, W.H., Chao, H.J., Wu, C.L., Huang, J.L., and Lee, M.S. (2012). Pilot scale production of highly efficacious and stable enterovirus 71 vaccine candidates. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0034834"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1038\/sj.gt.3300373","article-title":"Optimized conditions for the production of recombinant amphotropic retroviral vector preparations","volume":"4","author":"Kaptein","year":"1997","journal-title":"Gene Ther."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1002\/(SICI)1097-0290(19990620)63:6<654::AID-BIT3>3.0.CO;2-1","article-title":"Kinetics of retrovirus production and decay","volume":"63","author":"Davis","year":"1999","journal-title":"Biotechnol. Bioeng."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1126\/science.abc1932","article-title":"Development of an inactivated vaccine candidate for SARS-CoV-2","volume":"369","author":"Gao","year":"2020","journal-title":"Science"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/j.cell.2020.06.008","article-title":"Development of an Inactivated Vaccine Candidate, BBIBP-CorV, with Potent Protection against SARS-CoV-2","volume":"182","author":"Wang","year":"2020","journal-title":"Cell"}],"container-title":["Vaccines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-393X\/9\/6\/613\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:11:48Z","timestamp":1760163108000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-393X\/9\/6\/613"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,7]]},"references-count":39,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["vaccines9060613"],"URL":"https:\/\/doi.org\/10.3390\/vaccines9060613","relation":{},"ISSN":["2076-393X"],"issn-type":[{"value":"2076-393X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,7]]}}}