{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T11:02:40Z","timestamp":1769598160134,"version":"3.49.0"},"reference-count":59,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T00:00:00Z","timestamp":1675296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Gastrointestinal nematodes and protozoa and other parasite occurrences were evaluated in free-ranging wolf (Canis lupus italicus) and red fox (Vulpes vulpes) populations from natural and anthropized areas of Central Italy. Analyzed fecal samples were collected from 60 foxes and 40 wolves in the anthropized areas, and 41 foxes and 39 wolves in the natural areas. In foxes, hookworm infections (p &lt; 0.0001) were more frequently recorded in the anthropized environment, while coccidia (p &lt; 0.05) and Cryptosporidium spp. (p &lt; 0.0001) were more frequent in the natural area. In wolves, a higher frequency of hookworms (p &lt; 0.0001) was observed in natural areas, while coccidia were more common in the anthropized area (p &lt; 0.05). Moreover, in the natural environment, trichuroid nematodes (p &lt; 0.0001) were significantly more frequent in wolves than in foxes, while Cryptosporidium (p &lt; 0.001) and Giardia duodenalis (p &lt; 0.001) were more common in foxes. In the anthropic area, the occurrence of hookworms was found to be significantly higher in foxes (p &lt; 0.0001), while trichuroid nematodes were more common in wolves (p &lt; 0.0001). The obtained data are indicative of a different diffusion of specific parasite taxa in wolves and foxes living in the natural and\/or anthropized environments examined herein.<\/jats:p>","DOI":"10.3390\/vetsci10020108","type":"journal-article","created":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T01:53:54Z","timestamp":1675302834000},"page":"108","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Gray Wolf (Canis lupus italicus) and Red Fox (Vulpes vulpes) Parasite Survey in Anthropized and Natural Areas of Central Italy"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6459-2151","authenticated-orcid":false,"given":"Stefania","family":"Perrucci","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences, University of Pisa, Viale delle Piagge n. 2, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michela","family":"Maestrini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Pisa, Viale delle Piagge n. 2, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1571-3620","authenticated-orcid":false,"given":"Francesca","family":"Coppola","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Pisa, Viale delle Piagge n. 2, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matteo","family":"Di Marco","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Pisa, Viale delle Piagge n. 2, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessia Di","family":"Rosso","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Pisa, Viale delle Piagge n. 2, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6738-0699","authenticated-orcid":false,"given":"Maria Irene","family":"Pacini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Pisa, Viale delle Piagge n. 2, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Zintu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Pisa, Viale delle Piagge n. 2, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0808-3037","authenticated-orcid":false,"given":"Antonio","family":"Felicioli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Pisa, Viale delle Piagge n. 2, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"678","DOI":"10.3390\/ijerph6020678","article-title":"Parasite Zoonoses and Wildlife: Emerging Issues","volume":"6","author":"Thompson","year":"2009","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.vetpar.2015.04.022","article-title":"The role of wild canids and felids in spreading parasites to dogs and cats in Europe. Part I: Protozoa and tick-borne agents","volume":"213","author":"Otranto","year":"2015","journal-title":"Vet. Parasitol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.vetpar.2015.04.020","article-title":"The role of wild canids and felids in spreading parasites to dogs and cats in Europe. Part II: Helminths and arthropods","volume":"213","author":"Otranto","year":"2015","journal-title":"Vet. Parasitol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.ijppaw.2015.01.006","article-title":"The role of wildlife in the transmission of parasitic zoonoses in peri-urban and urban areas","volume":"4","author":"Mackenstedt","year":"2015","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e01892","DOI":"10.1016\/j.gecco.2021.e01892","article-title":"Men and wolves: Anthropogenic causes are an important driver of wolf mortality in human-dominated landscapes in Italy","volume":"32","author":"Musto","year":"2021","journal-title":"Glob. Ecol. Conserv."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1080\/03949370.2014.986768","article-title":"Short-term responses of wolf feeding habits to changes of wild and domestic ungulate abundance in Northern Italy","volume":"27","author":"Meriggi","year":"2014","journal-title":"Ethol. Ecol. Evol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1016\/j.ijpara.2005.07.003","article-title":"Navigating parasite webs and parasite flow: Emerging and re-emerging parasitic zoonoses of wildlife origin","volume":"35","author":"Polley","year":"2005","journal-title":"Int. J. Parasitol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"414","DOI":"10.2993\/0278-0771-40.4.414","article-title":"Dog-Human Coevolution: Cross-Cultural Analysis of Multiple Hypotheses","volume":"40","author":"Chambers","year":"2020","journal-title":"J. Ethnobiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1051\/parasite\/2021008","article-title":"Taeniid cestodes in a wolf pack living in a highly anthropic hilly agro-ecosystem","volume":"28","author":"Macchioni","year":"2021","journal-title":"Parasite"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e13799","DOI":"10.1111\/asj.13799","article-title":"Settlement of a stable wolf pack in a highly anthropic area of Pisan hills: Relationship with animal husbandry and hunting in a human\u2013wolf coexistence perspective","volume":"93","author":"Coppola","year":"2022","journal-title":"Anim. Sci. J."},{"key":"ref_11","unstructured":"(2023, January 08). Riserva Naturale di Monterufoli-Caselli. Available online: https:\/\/it.wikipedia.org\/wiki\/Riserva_naturale_di_Monterufoli-Caselli."},{"key":"ref_12","unstructured":"(2023, January 08). Parco Foreste Casentinesi. Available online: https:\/\/www.parcoforestecasentinesi.it\/."},{"key":"ref_13","first-page":"628","article-title":"Scent-marking in wolves","volume":"63","author":"Peters","year":"1975","journal-title":"Am. Sci."},{"key":"ref_14","unstructured":"Lang, A. (1989). Tracce di Animali. Impronte, Escrementi, Tracce di Pasti, Borre, Tane e Nidi, Zanichelli."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1590\/S0074-02762003000900014","article-title":"Terrestrial mammal feces: A morphometric summary and description","volume":"98","author":"Chame","year":"2003","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1038\/nprot.2017.067","article-title":"The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals","volume":"12","author":"Cringoli","year":"2017","journal-title":"Nat. Protoc."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Bowman, D.D., Hendrix, C.M., Lindsay, D.S., and Barr, S.C. (2002). Feline Clinical Parasitology, Iowa State University Press.","DOI":"10.1002\/9780470376805"},{"key":"ref_18","unstructured":"Traversa, D., and Venco, L. (2018). Parassitologia Clinica del Cane e del Gatto, Point V\u00e8t\u00e8rinaire Italie."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1186\/1756-3305-5-128","article-title":"Mixed trichuroid infestation in a dog from Italy","volume":"5","author":"Castagna","year":"2012","journal-title":"Parasites Vectors"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.vetpar.2009.04.005","article-title":"Physaloptera sibirica in foxes and badgers from the Western Alps (Italy)","volume":"163","author":"Ferroglio","year":"2009","journal-title":"Vet. Parasitol."},{"key":"ref_21","unstructured":"Taylor, M.A., Coop, R.L., and Wall, R.L. (2007). Veterinary Parasitology, Blackwell Publishing. [3rd ed.]."},{"key":"ref_22","first-page":"10","article-title":"Neospora caninum oocyst shedding in a naturally infected dog from Italy","volume":"8","author":"Perrucci","year":"2017","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_23","first-page":"e88","article-title":"Gastrointestinal helminths of wolves (Canis lupus Linnaeus, 1758) in Piedmont, north-western Italy","volume":"94","author":"Zanet","year":"2019","journal-title":"J. Helminthol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1007\/s00436-018-6181-3","article-title":"Helminth infections of wild European gray wolves (Canis lupus Linnaeus, 1758) in Lower Saxony, Germany, and comparison to captive wolves","volume":"118","author":"Bindke","year":"2019","journal-title":"Parasitol. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1080\/11250003.2016.1249966","article-title":"Helminth parasites of the red fox Vulpes vulpes (L., 1758) and the wolf Canis lupus italicus Altobello, 1921 in Emilia-Romagna, Italy","volume":"83","author":"Fiocchi","year":"2016","journal-title":"Ital. J. Zool."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1186\/s13071-018-3021-3","article-title":"Prevalence of intestinal helminths of red foxes (Vulpes vulpes) in central Europe (Poland): A significant zoonotic threat","volume":"11","author":"Karamon","year":"2018","journal-title":"Parasites Vectors"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"881","DOI":"10.7589\/0090-3558-45.3.881","article-title":"Endoparasites of Red Fox (Vulpes vulpes) in Central Italy","volume":"45","author":"Magi","year":"2009","journal-title":"J. Wildl. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Stufano, A., Foti, C., Lovreglio, P., Romita, P., De Marco, A., Lia, R.P., Otranto, D., and Iatta, R. (2022). Occupational risk of cutaneous larva migrans: A case report and a systematic literature review. PLOS Neglected Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010330"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Mech, L.D., Smith, D.W., and MacNulty, D.R. (2015). Wolves on the Hunt: The Behavior Of Wolves Hunting Wild Prey, University of Chicago Press.","DOI":"10.7208\/chicago\/9780226255286.001.0001"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1093\/cz\/zox031","article-title":"Wolf predation on wild ungulates: How slope and habitat cover influence the localization of kill sites","volume":"64","author":"Torretta","year":"2017","journal-title":"Curr. Zo\u00f6l."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1007\/s11252-020-00948-w","article-title":"Feeding behaviour of red fox and domestic cat populations in suburban areas in the south of Paris","volume":"23","author":"Bonnaud","year":"2020","journal-title":"Urban Ecosyst."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4229","DOI":"10.1007\/s00436-021-07073-3","article-title":"Predators in northern Germany are reservoirs for parasites of One Health concern","volume":"120","author":"Waindok","year":"2021","journal-title":"Parasitol. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1017\/S0022149X1400025X","article-title":"Extraintestinal nematodes of the red fox Vulpes vulpes in north-west Italy","volume":"89","author":"Magi","year":"2014","journal-title":"J. Helminthol."},{"key":"ref_34","first-page":"302","article-title":"Intestinal helminth communities of the red fox (Vulpes vulpes L.) in the Italian Alps","volume":"53","author":"Manfredi","year":"2008","journal-title":"Acta Parasitol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/1756-3305-3-62","article-title":"Canine and feline cardiopulmonary parasitic nematodes in Europe: Emerging and underestimated","volume":"3","author":"Traversa","year":"2010","journal-title":"Parasites Vectors"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4463","DOI":"10.1007\/s00436-015-4687-5","article-title":"Respiratory nematodes in cat populations of Italy","volume":"114","author":"Veronesi","year":"2015","journal-title":"Parasitol. Res."},{"key":"ref_37","first-page":"100321","article-title":"Gastrointestinal parasites in shelter cats of central Italy","volume":"18","author":"Sauda","year":"2019","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.vetpar.2016.04.016","article-title":"Red fox (Vulpes vulpes) as a potential reservoir host of cardiorespiratory parasites in Bosnia and Herzegovina","volume":"223","author":"Brianti","year":"2016","journal-title":"Vet. Parasitol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1007\/s00436-007-0520-0","article-title":"Influence of urbanization on the epidemiology of intestinal helminths of the red fox (Vulpes vulpes) in Geneva, Switzerland","volume":"101","author":"Reperant","year":"2007","journal-title":"Parasitol. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.pt.2009.01.006","article-title":"How common is human toxocariasis? Towards standardizing our knowledge","volume":"25","author":"Smith","year":"2009","journal-title":"Trends Parasitol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s40249-018-0437-0","article-title":"Toxocariasis: A silent threat with a progressive public health impact","volume":"7","author":"Chen","year":"2018","journal-title":"Infect. Dis. Poverty"},{"key":"ref_42","first-page":"465","article-title":"Spirocerca lupi isolated from gastric lesions in foxes (Vulpes vulpes) in Sicily (Italy)","volume":"13","author":"Ferrantelli","year":"2010","journal-title":"Pol. J. Vet. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1017\/S0031182018000707","article-title":"Spirocerca vulpis sp. nov. (Spiruridae: Spirocercidae): Description of a new nematode species of the red fox, Vulpes vulpes (Carnivora: Canidae)","volume":"145","author":"Rojas","year":"2018","journal-title":"Parasitology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3105","DOI":"10.1007\/s00436-020-06791-4","article-title":"Unravelling Spirocerca vulpis from red foxes from Switzerland: A 20-year-old record","volume":"119","author":"Rojas","year":"2020","journal-title":"Parasitol. Res."},{"key":"ref_45","first-page":"840","article-title":"Physaloptera stomach worms associated with chronic vomition in a dog in Western Canada","volume":"31","author":"Clark","year":"1990","journal-title":"Am. Jew. Hist."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"73","DOI":"10.2478\/s11687-009-0015-x","article-title":"Endoparasites of red fox (Vulpes vulpes) in the Slovak Republic with the emphasis on zoonotic species Echinococcus multilocularis and Trichinella spp.","volume":"46","author":"Miterpakova","year":"2009","journal-title":"Helminthologia"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.vetpar.2016.11.011","article-title":"Protozoan and helminth parasite fauna of free-living Croatian wild wolves (Canis lupus) analyzed by scat collection","volume":"233","author":"Hermosilla","year":"2017","journal-title":"Vet. Parasitol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Guadano Procesi, I., Montalbano Di Filippo, M., De Liberato, C., Lombardo, A., Brocherel, G., Perrucci, S., Di Cave, D., and Berrilli, F. (2022). Giardia duodenalis in Wildlife: Exploring Genotype Diversity in Italy and across Europe. Pathogens, 11.","DOI":"10.3390\/pathogens11010105"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1007\/s10344-019-1326-y","article-title":"Detection of selected pathogens in Apennine wolf (Canis lupus italicus) by a non-invasive GPS-based telemetry sampling of two packs from Majella National Park, Italy","volume":"65","author":"Smoglica","year":"2019","journal-title":"Eur. J. Wildl. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"101","DOI":"10.2478\/macvetrev-2019-0013","article-title":"Giardia and Cryptosporidium in Red Foxes (Vulpes Vulpes): Screening for Coproantigens in a Population of Central Italy and Mini-Review of the Literature","volume":"42","author":"Papini","year":"2019","journal-title":"Maced. Vet. Rev."},{"key":"ref_51","first-page":"265","article-title":"Distribution of Cryptosporidium and Giardia spp. in selected species of protected and game mammals from North-Eastern Poland","volume":"14","author":"Paziewska","year":"2007","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.vetpar.2017.01.016","article-title":"Occurrence and molecular genotyping of Giardia duodenalis and Cryptosporidium spp. in wild mesocarnivores in Spain","volume":"235","author":"Mateo","year":"2017","journal-title":"Vet. Parasitol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1089\/vbz.2010.0113","article-title":"A Large Survey of Croatian Wild Mammals for Giardia duodenalis Reveals a Low Prevalence and Limited Zoonotic Potential","volume":"11","author":"Beck","year":"2011","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_54","first-page":"9","article-title":"Osservazioni sulla coccidiosi delle volpi in Abruzzo","volume":"7","author":"Mantovani","year":"1965","journal-title":"Parassitologia"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3587","DOI":"10.1007\/s00436-013-3544-7","article-title":"A coprological survey of parasites of wild carnivores in Ireland","volume":"112","author":"Stuart","year":"2013","journal-title":"Parasitol. Res."},{"key":"ref_56","first-page":"112","article-title":"Red foxes, an important source of zoonotic parasites in Romania","volume":"16","author":"Onac","year":"2015","journal-title":"Sci. Parasitol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"10338","DOI":"10.1002\/ece3.7837","article-title":"Cross-continental comparison of parasite communities in a wide-ranging carnivore suggests associations with prey diversity and host density","volume":"11","author":"Stronen","year":"2021","journal-title":"Ecol. Evol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.ijppaw.2016.04.002","article-title":"Parasite species of the endangered Iberian wolf (Canis lupus signatus) and a sympatric widespread carnivore","volume":"5","author":"Figueiredo","year":"2016","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.ijppaw.2016.09.003","article-title":"Rangelia vitalii in a free-ranging maned wolf (Chrysocyon brachyurus) and co-infections","volume":"5","author":"Silveira","year":"2016","journal-title":"Int. J. Parasitol. Parasites Wildl."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/2\/108\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:21:44Z","timestamp":1760120504000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/2\/108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,2]]},"references-count":59,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["vetsci10020108"],"URL":"https:\/\/doi.org\/10.3390\/vetsci10020108","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,2]]}}}