{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T16:34:41Z","timestamp":1763829281679,"version":"build-2065373602"},"reference-count":31,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T00:00:00Z","timestamp":1676851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"JSPS KAKENHI","doi-asserted-by":"publisher","award":["20K15660","22K19242","Program on Open Innovation Platform with Enterprises, Research Institute and Academia (OPERA)"],"award-info":[{"award-number":["20K15660","22K19242","Program on Open Innovation Platform with Enterprises, Research Institute and Academia (OPERA)"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["20K15660","22K19242","Program on Open Innovation Platform with Enterprises, Research Institute and Academia (OPERA)"],"award-info":[{"award-number":["20K15660","22K19242","Program on Open Innovation Platform with Enterprises, Research Institute and Academia (OPERA)"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Amyloid-producing ameloblastoma (APAB) is characterized by abundant amyloid deposits in ameloblastoma, but the amyloid precursor protein is unknown. To explore this, we conducted histopathologic and proteomic analyses on formalin-fixed and paraffin-embedded samples from five cases of APAB (three dogs and two cats). Histologically, the samples exhibited a proliferation of the odontogenic epithelium, with moderate to severe interstitial amyloid deposits. By using Congo red and polarized light, the amyloid deposits were found to show characteristic birefringence. Amyloid deposits were dissected from tissue sections and analyzed by LC\/MS\/MS, and high levels of ameloblastin were detected in all tissues. Mass spectrometry also revealed that the N-terminal region of ameloblastin is predominantly present in amyloid deposits. Immunohistochemistry was performed using two anti-ameloblastin (N terminal, middle region) antibodies and showed that amyloid deposits were positive for ameloblastin N terminal but negative for ameloblastin middle region. These results suggest that ameloblastin is the amyloid precursor protein of APABs in dogs and cats, and the N-terminal region may be involved in the amyloidogenesis of ameloblastin.<\/jats:p>","DOI":"10.3390\/vetsci10020166","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T02:29:08Z","timestamp":1676860148000},"page":"166","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identification of Ameloblastin as an Amyloid Precursor Protein of Amyloid-Producing Ameloblastoma in Dogs and Cats"],"prefix":"10.3390","volume":"10","author":[{"given":"Niki Sedghi","family":"Masoud","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Toxicology, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susumu","family":"Iwaide","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Toxicology, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiyuki","family":"Itoh","sequence":"additional","affiliation":[{"name":"Smart-Core-Facility Promotion Organization, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miki","family":"Hisada","sequence":"additional","affiliation":[{"name":"Smart-Core-Facility Promotion Organization, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9132-9033","authenticated-orcid":false,"given":"Tomoyuki","family":"Harada","sequence":"additional","affiliation":[{"name":"FUJIFILM VET Systems Co., Ltd., Tokyo 185-0013, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5706-7842","authenticated-orcid":false,"given":"Tomoaki","family":"Murakami","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Toxicology, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1159\/000506696","article-title":"The Pathology of Amyloidosis in Classification: A Review","volume":"143","author":"Picken","year":"2020","journal-title":"Acta Haematol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1080\/13506129.2022.2147636","article-title":"Amyloid nomenclature 2022: Update, novel proteins, and recommendations by the International Society of Amyloidosis (ISA) Nomenclature Committee","volume":"29","author":"Buxbaum","year":"2022","journal-title":"Amyloid"},{"key":"ref_3","unstructured":"Kumar, V., Abbas, A.K., Fausto, N., and Mitchell, R.N. 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