{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T18:08:25Z","timestamp":1774634905082,"version":"3.50.1"},"reference-count":36,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,28]],"date-time":"2023-02-28T00:00:00Z","timestamp":1677542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hellenic Society of Veterinary Dermatology (HSVD)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Rapid diagnosis of dermatophytosis is essential for early treatment induction and prevention of spreading to other animals and humans. No single diagnostic test is identified as the \u201cgold standard\u201d. The purpose of the study was to evaluate the sensitivity of adhesive tape impression (ATI) cytology in dermatophyte identification and to compare three diagnostic tests for dermatophytosis. Thirty dogs, with alopecia (n = 19) or kerion (n = 11), and fifteen cats with alopecia were included in the study. Dermatophytosis was diagnosed with tape preparations in 82.2% (37\/45) of cases, while with hair plucks in 66.7% (30\/45) and fungal culture in 80% (36\/45). In kerions, tape preparations and fungal culture had the same sensitivity (10\/11, 90.9%) that was higher than that of hair plucks (4\/11, 36.4%). The sensitivity was higher in cats than in dogs with alopecia for all tests, 80% versus (vs.) 73.7%, 86.7% vs. 68.4%, and 93.3% vs. 68.4% for cats and dogs for hair plucks, fungal culture, and tape preparations, respectively. No significant difference was found between the three tests, except for dogs with kerion. Hair plucks were less sensitive than fungal culture in kerions (p = 0.041), while in comparison with tape preparations they were marginally not significantly different (p = 0.078). ATI cytology is a useful diagnostic test in dermatophytosis, in dogs with kerion as well as cats.<\/jats:p>","DOI":"10.3390\/vetsci10030183","type":"journal-article","created":{"date-parts":[[2023,2,28]],"date-time":"2023-02-28T03:00:38Z","timestamp":1677553238000},"page":"183","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of Adhesive Tape Impression Cytology, Hair Plucks, and Fungal Culture for the Diagnosis of Dermatophytosis in Dogs and Cats"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4140-5683","authenticated-orcid":false,"given":"Pavlina","family":"Bouza-Rapti","sequence":"first","affiliation":[{"name":"Companion Animal Clinic, Medicine Unit, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54627 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia","family":"Karafylia","sequence":"additional","affiliation":[{"name":"Companion Animal Clinic, Medicine Unit, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54627 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2618-8668","authenticated-orcid":false,"given":"Androniki","family":"Tamvakis","sequence":"additional","affiliation":[{"name":"Laboratory of Ecology and System Dynamics, Department of Marine Sciences, University of the Aegean, 81100 Mytilene, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rania","family":"Farmaki","sequence":"additional","affiliation":[{"name":"Companion Animal Clinic, Medicine Unit, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54627 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1007\/s11046-008-9102-7","article-title":"Dermatophytoses in animals","volume":"166","author":"Chermette","year":"2008","journal-title":"Mycopathologia"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.clindermatol.2009.12.012","article-title":"Superficial veterinary mycoses","volume":"28","author":"Bond","year":"2010","journal-title":"Clin. Dermatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"266-e68","DOI":"10.1111\/vde.12440","article-title":"Diagnosis and treatment of dermatophytosis in dogs and cats. Clinical Consensus Guidelines of the World Association for Veterinary Dermatology","volume":"28","author":"Moriello","year":"2017","journal-title":"Vet. Dermatol."},{"key":"ref_4","first-page":"257","article-title":"Dermatophytosis in dogs and cats- an update","volume":"47","author":"Boehm","year":"2019","journal-title":"Tierarztl. Prax. Ausg. K Kleintiere Heimtiere"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Moskaluk, A.E., and VandeWoude, S. (2022). Current Topics in Dermatophyte Classification and Clinical Diagnosis. Pathogens, 11.","DOI":"10.3390\/pathogens11090957"},{"key":"ref_6","first-page":"1217","article-title":"Feline dermatophytosis: Clinical features and diagnostic testing","volume":"61","author":"Bajwa","year":"2020","journal-title":"Can. Vet. J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1111\/j.1365-3164.2009.00749.x","article-title":"Canine nodular dermatophytosis (kerion): 23 cases","volume":"20","author":"Cornegliani","year":"2009","journal-title":"Vet. Dermatol."},{"key":"ref_8","first-page":"27","article-title":"Comparison of two sampling methods for microscopic examination of hair shafts in feline and canine dermatophytosis","volume":"24","author":"Colombo","year":"2010","journal-title":"Veterinaria"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1046\/j.1365-3164.1999.00115.x","article-title":"Dermatophytosis due to Microsporum persicolor (13 cases) or Microsporum gypseum (20 cases) in dogs","volume":"10","author":"Carlotti","year":"1999","journal-title":"Vet. Dermatol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"284-e68","DOI":"10.1111\/vde.12322","article-title":"Comparison between point-of-care dermatophyte test medium and mycology laboratory culture for diagnosis of dermatophytosis in dogs and cats","volume":"27","author":"Kaufmann","year":"2016","journal-title":"Vet. Dermatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1111\/myc.12821","article-title":"Assessment of a pan-dermatophyte nested-PCR compared with conventional methods for direct detection and identification of dermatophytosis agents in animals","volume":"61","author":"Piri","year":"2018","journal-title":"Mycoses"},{"key":"ref_12","first-page":"596","article-title":"Comparison of acetate tape impression, deep skin scraping, and microscopic examination of hair for therapeutic monitoring of dogs with juvenile generalized demodicosis: A pilot study","volume":"60","author":"Barillas","year":"2019","journal-title":"Can. Vet. J."},{"key":"ref_13","first-page":"45 (abstract)","article-title":"Adhesive tape impression cytology in dermatophytosis and pemphigus foliaceus. In Special Issue: Abstracts from the 9th World Congress of Veterinary Dermatology, October 2020\u2013April 2021, Sydney, Australia","volume":"31","author":"Vogelnest","year":"2020","journal-title":"Vet. Dermatol."},{"key":"ref_14","unstructured":"Miller, W.H., Griffin, C.E., and Campbell, K.L. (2013). Muller & Kirk\u2019s Small Animal Dermatology, Elsevier. [7th ed.]."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.2460\/javma.244.11.1304","article-title":"Malassezia spp. on the periocular skin of dogs and their association with blepharitis, ocular discharge, and the application of ophthalmic medications","volume":"244","author":"Newbold","year":"2014","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"279-e67","DOI":"10.1111\/vde.12297","article-title":"Evaluation of cytology collection techniques and prevalence of Malassezia yeast and bacteria in claw folds of normal and allergic dogs","volume":"27","author":"Lo","year":"2016","journal-title":"Vet. Dermatol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"193","DOI":"10.5326\/JAAHA-MS-6458","article-title":"Impression smear agreement with acetate tape preparation for cytologic sampling","volume":"53","author":"Layne","year":"2017","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_18","unstructured":"Albanese, F. (2017). Canine and Feline Skin Cytology: A Comprehensive and Illustrated Guide to the Interpretation of Skin Lesions via Cytological Examination, Springer. [1st ed.]."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1111\/j.1751-0813.2012.00994.x","article-title":"Comparison of acetate tape impression with squeezing versus skin scraping for the diagnosis of canine demodicosis","volume":"90","author":"Pereira","year":"2012","journal-title":"Aust. Vet. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1177\/1098612X16632279","article-title":"Acetate tape impression test for diagnosis of notoedric mange in cats","volume":"19","author":"Sampaio","year":"2017","journal-title":"J. Feline Med. Surg."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4-e2","DOI":"10.1111\/vde.12806","article-title":"Diagnosis and treatment of demodicosis in dogs and cats: Clinical consensus guidelines of the World Association for Veterinary Dermatology","volume":"31","author":"Mueller","year":"2020","journal-title":"Vet. Dermatol."},{"key":"ref_22","unstructured":"R Core Team (2022, December 01). R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria 2020. Available online: https:\/\/www.R-project.org\/."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/j.1365-3164.1991.tb00111.x","article-title":"Epidemiology and clinical features of dermatophytosis in dogs and cats at Louisiana State University: 1981\u20131990","volume":"2","author":"Lewis","year":"1991","journal-title":"Vet. Dermatol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1023\/A:1021361001794","article-title":"Dermatophytes isolated from symptomatic dogs and cats in Tuscany, Italy during a 15-year-period","volume":"156","author":"Mancianti","year":"2002","journal-title":"Mycopathologia"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.prevetmed.2010.11.003","article-title":"Isolation of dermatophytes from dogs and cats with suspected dermatophytosis in Western Turkey","volume":"98","author":"Seker","year":"2011","journal-title":"Prev. Vet. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"39","DOI":"10.3314\/mmj.19.001","article-title":"An epidemiological study of feline and canine dermatophytoses in Japan","volume":"60","author":"Yamada","year":"2019","journal-title":"Med. Mycol. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e87","DOI":"10.1136\/vr.105957","article-title":"Isolation of dermatophytes from dogs and cats in the South of England between 1991 and 2017","volume":"187","author":"Long","year":"2020","journal-title":"Vet. Rec."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1111\/j.1439-0507.2004.01055.x","article-title":"The epidemiology of canine and feline dermatophytoses in southern Italy","volume":"47","author":"Cafarchia","year":"2004","journal-title":"Mycoses"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1590\/S0103-84782011000800017","article-title":"A retrospective of dermatophytosis in dogs and cats Veterinary Hospital at the Universidade Federal de Mato Grosso, in the years 2006 to 2008","volume":"41","author":"Lima","year":"2011","journal-title":"Cienc. Rural"},{"key":"ref_30","first-page":"639","article-title":"Mixed dermatophyte infection in a cat","volume":"47","year":"2001","journal-title":"Wiad. Parazytol."},{"key":"ref_31","first-page":"243 (abstract)","article-title":"Clinical and histopathological aspects of dermatophyte kerion in the dog: A retrospective study of 20 spontaneous cases. In ESVD and ECVD 2002 Abstracts (Nice, France)","volume":"14","author":"Koutinas","year":"2003","journal-title":"Vet. Dermatol."},{"key":"ref_32","first-page":"9","article-title":"Il kerio dermatofico: Aspetti eziologici, clinici, diagnostici e terapeutici in 39 cani","volume":"21","author":"Albanese","year":"2007","journal-title":"Veterinaria"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1177\/1098612X19825519","article-title":"Descriptive epidemiology and test characteristics of cats diagnosed with Microsporum canis dermatophytosis in a Northwestern US animal shelter","volume":"21","author":"DeTar","year":"2019","journal-title":"J. Feline Med. Surg."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"282-e63","DOI":"10.1111\/vde.12212","article-title":"Dermoscopic features in 12 cats with dermatophytosis and in 12 cats with self-induced alopecia due to other causes: An observational descriptive study","volume":"26","author":"Scarampella","year":"2015","journal-title":"Vet. Dermatol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"275-e65","DOI":"10.1111\/vde.12333","article-title":"Evaluation of dermoscopy in the diagnosis of naturally occurring dermatophytosis in cats","volume":"27","author":"Dong","year":"2016","journal-title":"Vet. Dermatol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Gross, T.L., Ihrke, P.J., Walder, E.J., and Affolter, V.K. (2005). Skin Diseases of the Dog and the Cat: Clinical and Histopathologic Diagnosis, Blackwell Science Ltd.. [2nd ed.].","DOI":"10.1002\/9780470752487"}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/3\/183\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:43:50Z","timestamp":1760121830000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/3\/183"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,28]]},"references-count":36,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["vetsci10030183"],"URL":"https:\/\/doi.org\/10.3390\/vetsci10030183","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,28]]}}}