{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:14:37Z","timestamp":1789755277902,"version":"4.0.1"},"reference-count":33,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,25]],"date-time":"2023-03-25T00:00:00Z","timestamp":1679702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The histopathological diagnosis of canine splenic mass lesions is crucial for prognostication. However, thus far, no study has been conducted on the histopathology of canine splenic mass lesions in Republic of Korea. Herein, the prevalence of splenic diseases was analyzed in 137 canine splenic mass lesions via histopathological diagnosis, and the microscopic pattern associated with each disorder was described. Immunohistochemistry was performed for CD31, CD3, PAX5, Iba1, and C-kit for a more accurate diagnosis of splenic tumors. The proportion of non-neoplastic disorders, including nodular hyperplasia (48.2%, n = 66) and hematoma (24.1%, n = 33), was 72.3%. Splenic tumors, including splenic hemangiosarcoma (10.2%, n = 14), splenic lymphoma (nodular and diffuse types, 8.0%, n = 11), splenic stromal sarcoma (7.3%, n = 10), myelolipoma (1.5%, n = 2), and mast cell tumors (0.7%, n = 1), accounted for 27.7% of cases. The results of this study will aid veterinary clinicians in communication with pet owners about prognoses, recommendations for splenectomy, and subsequent histopathological diagnoses. This study will facilitate further investigations with more detailed comparisons of splenic mass lesions between small- and large-breed dogs.<\/jats:p>","DOI":"10.3390\/vetsci10040247","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T02:18:27Z","timestamp":1679883507000},"page":"247","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Analysis of the Prevalence of Canine Splenic Mass Lesions in Republic of Korea via Histopathological Diagnosis with Immunohistochemistry"],"prefix":"10.3390","volume":"10","author":[{"given":"Yeong-Ung","family":"Ko","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathology, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea"},{"name":"Neodin Biovet Laboratory, Achiul-gil, Guri-si 11956, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Kyung","family":"Bae","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea"},{"name":"Neodin Biovet Laboratory, Achiul-gil, Guri-si 11956, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung-Hyang","family":"Sur","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea"},{"name":"Neodin Biovet Laboratory, Achiul-gil, Guri-si 11956, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nong-Hoon","family":"Choe","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"231","DOI":"10.3109\/01902148109052318","article-title":"The Effect of Splenectomy on the Appearance of Specific Antibody-Forming Cells in Lungs of Dogs after Intravenous Immunization with Sheep Erythrocytes","volume":"2","author":"Kaltreider","year":"1981","journal-title":"Exp. Lung Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1111\/j.1751-0813.2009.00421.x","article-title":"Cytopathological and Histopathological Diagnosis of Canine Splenic Disorders","volume":"87","author":"Christensen","year":"2009","journal-title":"Aust. Vet. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"690","DOI":"10.2460\/javma.230.5.690","article-title":"Correlation of Ultrasonographic Appearance of Lesions and Cytologic and Histologic Diagnoses in Splenic Aspirates from Dogs and Cats: 32 Cases (2002\u20132005)","volume":"230","author":"Ballegeer","year":"2007","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1111\/j.1748-5827.1995.tb02769.x","article-title":"A Review of Pathological Diagnoses Made from 87 Canine Splenic Biopsies","volume":"36","author":"Day","year":"1995","journal-title":"J. Small Anim. Pract."},{"key":"ref_5","first-page":"222","article-title":"Advantages and Disadvantages of Cytology and Histopathology for the Diagnosis of Cancer","volume":"8","author":"Morrison","year":"1993","journal-title":"Semin. Vet. Med. Surg. Small Anim."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"827","DOI":"10.4142\/jvs.2018.19.6.827","article-title":"Presurgical Assessment of Splenic Tumors in Dogs: A Retrospective Study of 57 Cases (2012\u20132017)","volume":"19","author":"Lee","year":"2018","journal-title":"J. Vet. Sci."},{"key":"ref_7","first-page":"56","article-title":"Canine Hemangiosarcoma: Retrospective Analysis of 104 Cases","volume":"186","author":"Brown","year":"1985","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1111\/vsu.12405","article-title":"Outcome and Prognostic Factors for Canine Splenic Lymphoma Treated by Splenectomy (1995\u20132011)","volume":"44","author":"Boston","year":"2015","journal-title":"Vet. Surg."},{"key":"ref_9","first-page":"706","article-title":"Epidemiologic, Clinical, Pathologic, and Prognostic Characteristics of Splenic Hemangiosarcoma and Splenic Hematoma in Dogs: 217 Cases (1985)","volume":"193","author":"Prymak","year":"1988","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1111\/j.1939-1676.1989.tb03092.x","article-title":"Splenomegaly in Dogs: Predictors of Neoplasia and Survival After Splenectomy","volume":"3","author":"Johnson","year":"1989","journal-title":"J. Vet. Intern. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"829","DOI":"10.2460\/javma.1992.200.06.829","article-title":"Prevalence, Type, and Importance of Splenic Diseases in Dogs: 1480 Cases (1985\u20131989)","volume":"200","author":"Spangler","year":"1992","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"518","DOI":"10.5326\/0390518","article-title":"Acute Nontraumatic Hemoabdomen in the Dog: A Retrospective Analysis of 39 Cases (1987\u20132001)","volume":"39","author":"Pintar","year":"2003","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.1111\/vsu.13470","article-title":"Outcomes of 43 Small Breed Dogs Treated for Splenic Hemangiosarcoma","volume":"49","author":"Story","year":"2020","journal-title":"Vet. Surg."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1111\/vco.12574","article-title":"Refining the \u201cDouble Two-Thirds\u201d Rule: Genotype-Based Breed Grouping and Clinical Presentation Help Predict the Diagnosis of Canine Splenic Mass Lesions in 288 Dogs","volume":"18","author":"Davies","year":"2020","journal-title":"Vet. Comp. Oncol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Meuten, D.J. (2017). Tumors in Domestic Animals, Wiley\/Blackwell. [5th ed.].","DOI":"10.1002\/9781119181200"},{"key":"ref_16","first-page":"101","article-title":"Canine Splenic Pathology: A Retrospective Study of 109 Surgical Samples, with Special Emphasis on Fibrohistiocytic Nodules","volume":"7","author":"Bettini","year":"2001","journal-title":"Eur. J. Vet. Pathol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"201","DOI":"10.5326\/JAAHA-MS-6934","article-title":"Evaluation of Nodular Splenic Lesions in 370 Small-Breed Dogs (<15 Kg)","volume":"55","author":"Fernandez","year":"2019","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.2460\/javma.250.10.1148","article-title":"Splenomegaly in Small-breed Dogs: 45 Cases (2005\u20132011)","volume":"250","author":"Corbin","year":"2017","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1111\/vco.12621","article-title":"Prospective Observational Study of Dogs with Splenic Mass Rupture Suggests Potentially Lower Risk of Malignancy and More Favourable Perioperative Outcomes","volume":"18","author":"Stewart","year":"2020","journal-title":"Vet. Comp. Oncol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"O\u2019Byrne, K., and Hosgood, G. (2019). Splenic Mass Diagnosis in Dogs Undergoing Splenectomy According to Breed Size. Vet. Rec., 184.","DOI":"10.1136\/vr.104983"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e5161","DOI":"10.7717\/peerj.5161","article-title":"Major Medical Causes by Breed and Life Stage for Dogs Presented at Veterinary Clinics in the Republic of Korea: A Survey of Electronic Medical Records","volume":"2018","author":"Kim","year":"2018","journal-title":"PeerJ"},{"key":"ref_22","first-page":"842","article-title":"Outcome and Prognostic Factors for Dogs with a Histological Diagnosis of Splenic Hematoma Following Splenectomy: 35 Cases (2001\u20132013)","volume":"57","author":"Patten","year":"2016","journal-title":"Can. Vet. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1292\/jvms.17-0561","article-title":"Cellular Atypia Is Negatively Correlated with Immunohistochemical Reactivity of CD31 and VWF Expression Levels in Canine Hemangiosarcoma","volume":"80","author":"Maharani","year":"2018","journal-title":"J. Vet. Med. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.jcpa.2012.11.234","article-title":"Canine Splenic Haemangiosarcoma: Influence of Metastases, Chemotherapy and Growth Pattern on Post-Splenectomy Survival and Expression of Angiogenic Factors","volume":"149","author":"Krastel","year":"2013","journal-title":"J. Comp. Pathol."},{"key":"ref_25","first-page":"372","article-title":"Immunohistochemical Characterization of Hemangiosarcoma in Dogs","volume":"67","author":"Tabaran","year":"2010","journal-title":"Vet. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1111\/vco.12259","article-title":"Use of Routine Histopathology and Factor VIII-Related Antigen\/von Willebrand Factor Immunohistochemistry to Differentiate Primary Hemangiosarcoma of Bone from Telangiectatic Osteosarcoma in 54 Dogs","volume":"15","author":"Giuffrida","year":"2017","journal-title":"Vet. Comp. Oncol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1111\/j.1365-2559.2006.02378.x","article-title":"Ki67 Staining Pattern as a Diagnostic Tool in the Evaluation of Lymphoproliferative Disorders","volume":"48","author":"Bryant","year":"2006","journal-title":"Histopathology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1111\/jvim.12185","article-title":"Lymphoma Immunophenotype of Dogs Determined by Immunohistochemistry, Flow Cytometry, and Polymerase Chain Reaction for Antigen Receptor Rearrangements","volume":"27","author":"Thalheim","year":"2013","journal-title":"J. Vet. Intern. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1177\/0300985810379428","article-title":"Classification of Canine Malignant Lymphomas According to the World Health Organization Criteria","volume":"48","author":"Valli","year":"2011","journal-title":"Vet. Pathol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1354\/vp.43-3-241","article-title":"Canine Indolent Nodular Lymphoma","volume":"43","author":"Valli","year":"2006","journal-title":"Vet. Pathol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1177\/0300985813478210","article-title":"Canine Lymphomas: Association of Classification Type, Disease Stage, Tumor Subtype, Mitotic Rate, and Treatment With Survival","volume":"50","author":"Valli","year":"2013","journal-title":"Vet. Pathol."},{"key":"ref_32","first-page":"784","article-title":"Nonangiogenic and Nonlymphomatous Sarcomas of the Canine Spleen: 57 Cases (1975\u20131987)","volume":"195","author":"Weinstein","year":"1989","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1177\/030098589403100105","article-title":"Primary Mesenchymal (Nonangiomatous\/Nonlymphomatous) Neoplasms Occurring in the Canine Spleen: Anatomic Classification, Immunohistochemistry, and Mitotic Activity Correlated with Patient Survival","volume":"31","author":"Spangler","year":"1994","journal-title":"Vet. Pathol."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/4\/247\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:02:52Z","timestamp":1760122972000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/4\/247"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,25]]},"references-count":33,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["vetsci10040247"],"URL":"https:\/\/doi.org\/10.3390\/vetsci10040247","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,25]]}}}