{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T02:59:42Z","timestamp":1772593182039,"version":"3.50.1"},"reference-count":41,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T00:00:00Z","timestamp":1680739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Sichuan Province Science and Technology Planning Project","award":["2021YFSY0005"],"award-info":[{"award-number":["2021YFSY0005"]}]},{"name":"the Sichuan Province Science and Technology Planning Project","award":["2023NSFSC1216"],"award-info":[{"award-number":["2023NSFSC1216"]}]},{"name":"the Sichuan Province Science and Technology Planning Project","award":["2022M722300"],"award-info":[{"award-number":["2022M722300"]}]},{"name":"the Sichuan Province Science and Technology Planning Project","award":["XJ2022047"],"award-info":[{"award-number":["XJ2022047"]}]},{"name":"the Provincial Natural Science Foundation of Sichuan","award":["2021YFSY0005"],"award-info":[{"award-number":["2021YFSY0005"]}]},{"name":"the Provincial Natural Science Foundation of Sichuan","award":["2023NSFSC1216"],"award-info":[{"award-number":["2023NSFSC1216"]}]},{"name":"the Provincial Natural Science Foundation of Sichuan","award":["2022M722300"],"award-info":[{"award-number":["2022M722300"]}]},{"name":"the Provincial Natural Science Foundation of Sichuan","award":["XJ2022047"],"award-info":[{"award-number":["XJ2022047"]}]},{"name":"the China Postdoctoral Science Foundation","award":["2021YFSY0005"],"award-info":[{"award-number":["2021YFSY0005"]}]},{"name":"the China Postdoctoral Science Foundation","award":["2023NSFSC1216"],"award-info":[{"award-number":["2023NSFSC1216"]}]},{"name":"the China Postdoctoral Science Foundation","award":["2022M722300"],"award-info":[{"award-number":["2022M722300"]}]},{"name":"the China Postdoctoral Science Foundation","award":["XJ2022047"],"award-info":[{"award-number":["XJ2022047"]}]},{"name":"The Hong Kong Scholars Program 2022, China Postdoctoral Science Foundation","award":["2021YFSY0005"],"award-info":[{"award-number":["2021YFSY0005"]}]},{"name":"The Hong Kong Scholars Program 2022, China Postdoctoral Science Foundation","award":["2023NSFSC1216"],"award-info":[{"award-number":["2023NSFSC1216"]}]},{"name":"The Hong Kong Scholars Program 2022, China Postdoctoral Science Foundation","award":["2022M722300"],"award-info":[{"award-number":["2022M722300"]}]},{"name":"The Hong Kong Scholars Program 2022, China Postdoctoral Science Foundation","award":["XJ2022047"],"award-info":[{"award-number":["XJ2022047"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>NADC34-like porcine reproductive and respiratory syndrome virus first appeared in 2017 in a herd of pigs in Liaoning Province, China. The virus was subsequently found in other provinces. Given the potential for this virus to cause an epidemic, rapid, sensitive, and specific detection of NADC34-like PRRSV is required. The virus\u2019 ORF5 gene was artificially synthesized based on a Chinese reference strain, and specific primers\/probes for the ORF5 gene were designed. Then, the amplified target fragment was cloned into the pMD19-T vector, and a series of diluted recombinant plasmids were used to generate a standard curve. An optimized real-time TaqMan RT-PCR method was established. The method was highly specific for NADC34-like PRRSV, without cross-reactions with other non-targeted pig viruses. The detection limit of this assay was 101 copies\/\u03bcL. The method had an efficiency of 98.8%, a squared regression value (R2) of 0.999, and showed a linear range of 103\u2013108 copies\/\u03bcL of DNA per reaction. This method was shown to be analytically specific and sensitive with a low intra- and inter-assay coefficient of variation (&lt;1.40%). A total of 321 clinical samples were tested using the established method, and four were shown to be positive (1.24%). This study confirmed the existence of NADC34-like PRRSV and HP-PRRSV co-infection in Sichuan and provided a promising alternative tool for the rapid detection of NADC34-like PRRSV.<\/jats:p>","DOI":"10.3390\/vetsci10040279","type":"journal-article","created":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T05:21:23Z","timestamp":1680758483000},"page":"279","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Development of a Real-Time TaqMan RT-PCR Assay for the Detection of NADC34-like Porcine Reproductive and Respiratory Syndrome Virus"],"prefix":"10.3390","volume":"10","author":[{"given":"Teng","family":"Tu","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maonan","family":"Pang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1820-3685","authenticated-orcid":false,"given":"Dike","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"You","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xulong","family":"Wu","sequence":"additional","affiliation":[{"name":"Chengdu Agricultural College, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueping","family":"Yao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zexiao","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9446-8330","authenticated-orcid":false,"given":"Meishen","family":"Ren","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Law Sau Fai Institute for Advancing Translational Medicine in Bone and Joint Diseases (TMBJ), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China"},{"name":"Guangdong-Hong Kong-Macao Greater Bay Area International Research Platform for Aptamer-Based Translational Medicine and Drug Discovery (HKAP), Hong Kong SAR, China"},{"name":"Institute of Integrated Bioinformedicine and Translational Science (IBTS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiping","family":"Lu","sequence":"additional","affiliation":[{"name":"Law Sau Fai Institute for Advancing Translational Medicine in Bone and Joint Diseases (TMBJ), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China"},{"name":"Guangdong-Hong Kong-Macao Greater Bay Area International Research Platform for Aptamer-Based Translational Medicine and Drug Discovery (HKAP), Hong Kong SAR, China"},{"name":"Institute of Integrated Bioinformedicine and Translational Science (IBTS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7807-7695","authenticated-orcid":false,"given":"Ge","family":"Zhang","sequence":"additional","affiliation":[{"name":"Law Sau Fai Institute for Advancing Translational Medicine in Bone and Joint Diseases (TMBJ), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China"},{"name":"Guangdong-Hong Kong-Macao Greater Bay Area International Research Platform for Aptamer-Based Translational Medicine and Drug Discovery (HKAP), Hong Kong SAR, China"},{"name":"Institute of Integrated Bioinformedicine and Translational Science (IBTS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanyuan","family":"Yu","sequence":"additional","affiliation":[{"name":"Law Sau Fai Institute for Advancing Translational Medicine in Bone and Joint Diseases (TMBJ), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China"},{"name":"Guangdong-Hong Kong-Macao Greater Bay Area International Research Platform for Aptamer-Based Translational Medicine and Drug Discovery (HKAP), Hong Kong SAR, China"},{"name":"Institute of Integrated Bioinformedicine and Translational Science (IBTS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.3181\/0903-MR-94","article-title":"Coronavirus diversity, phylogeny and interspecies jumping","volume":"234","author":"Woo","year":"2009","journal-title":"Exp. 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