{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:14:02Z","timestamp":1789578842127,"version":"build-2803163510"},"reference-count":32,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T00:00:00Z","timestamp":1680739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>In the context of cost increases of both labor and consumables, cheaper and faster histopathology methods are needed. We implemented in our research laboratory the use of tissue microarrays (TMA) for the parallel processing and analysis of tissue samples. In this study, we used seven pre-processed, paraffinated biomimetic sectionable support matrices serving as \u201crecipient\u201d paraffin blocks to embed a total of 196 tissue cores from formalin-fixed paraffin-embedded tissue samples (serving as \u201cdonor\u201d paraffin blocks) from seven different rabbit organs. These tissue samples were obtained using four different processing protocols: two 6 h protocols with xylene as the transition solvent, and two using butanol instead (one 10 h in duration and the other 72 h long). While the samples from protocols 1 and 2 (with xylene) quite regularly generated peeling of some of the cores from the slides (most likely because of substandard paraffin infiltration), butanol processing performed flawlessly for both processing protocols. Our proposed technique of using TMAs in the research laboratory brings with it a significant reduction in time and consumable costs (up to 77 and 64%, respectively), but also new challenges for all the upstream processes.<\/jats:p>","DOI":"10.3390\/vetsci10040280","type":"journal-article","created":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T08:41:52Z","timestamp":1680770512000},"page":"280","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Quality Assurance and Cost Reduction in Histopathology Laboratories Using Tissue Microarrays"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0861-6857","authenticated-orcid":false,"given":"Daniela","family":"Gologan","sequence":"first","affiliation":[{"name":"Department of Organic Chemistry, Doctoral School, Faculty of Chemical Engineering and Biotechnologies, Politehnica University, 011061 Bucharest, Romania"},{"name":"Department of Research and Development, Themis Pathology SRL, 077190 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina Elena","family":"\u0218tefan","sequence":"additional","affiliation":[{"name":"Department of Research and Development, Themis Pathology SRL, 077190 Bucharest, Romania"},{"name":"Department of Pathology, Doctoral School, Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, 011464 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuella","family":"Militaru","sequence":"additional","affiliation":[{"name":"Department of Pathology, Doctoral School, Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, 011464 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreea Cristina","family":"Sanda","sequence":"additional","affiliation":[{"name":"Department of Research and Development, Themis Pathology SRL, 077190 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzana","family":"Arjan","sequence":"additional","affiliation":[{"name":"Department of Research and Development, Themis Pathology SRL, 077190 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sorin","family":"Mu\u0219at","sequence":"additional","affiliation":[{"name":"Department of Research and Development, Themis Pathology SRL, 077190 Bucharest, Romania"},{"name":"Department of Research and Development, LUMEA Inc., Lehi, UT 84043, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew Okerlund","family":"Leavitt","sequence":"additional","affiliation":[{"name":"Department of Research and Development, LUMEA Inc., Lehi, UT 84043, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raluca","family":"Stan","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, Doctoral School, Faculty of Chemical Engineering and Biotechnologies, Politehnica University, 011061 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1093\/ajcp\/aqz101","article-title":"Prostate Biopsy Processing: An Innovative Model for Reducing Cost, Decreasing Test Time, and Improving Diagnostic Material","volume":"152","author":"Murugan","year":"2019","journal-title":"Am. 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