{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T14:19:36Z","timestamp":1782742776747,"version":"3.54.5"},"reference-count":32,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,4,25]],"date-time":"2023-04-25T00:00:00Z","timestamp":1682380800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation (DFG)","doi-asserted-by":"publisher","award":["FU 1027\/3-1"],"award-info":[{"award-number":["FU 1027\/3-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation (DFG)","doi-asserted-by":"publisher","award":["FU 1027\/5-1"],"award-info":[{"award-number":["FU 1027\/5-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation (DFG)","doi-asserted-by":"publisher","award":["S20-851-890"],"award-info":[{"award-number":["S20-851-890"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Petplan Charitable Trust foundation","award":["FU 1027\/3-1"],"award-info":[{"award-number":["FU 1027\/3-1"]}]},{"name":"Petplan Charitable Trust foundation","award":["FU 1027\/5-1"],"award-info":[{"award-number":["FU 1027\/5-1"]}]},{"name":"Petplan Charitable Trust foundation","award":["S20-851-890"],"award-info":[{"award-number":["S20-851-890"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>An 8-year-old male Rhodesian Ridgeback was presented with fever and severe thrombocytopenia. Clinical and laboratory examination, echocardiography, blood culture, and pathohistology revealed evidence of infective endocarditis, ischemic renal infarcts, and septic encephalitis. Treatment was started immediately but the dog\u2019s condition worsened, and the dog had to be euthanized. The causative Streptococcus canis strain was detected by blood culture and MALDI-TOF MS and analyzed using whole-genome sequencing and multilocus sequence typing. Antibiotic susceptibility testing did not detect any resistance. The affected heart valve was analyzed using FISH imaging, which showed a streptococcal biofilm on the heart valve. Bacteria in biofilms are recalcitrant to antibiotic treatment. Early diagnosis could be beneficial to treatment outcome. Treatment of endocarditis could be improved by researching the optimal dosage of antibiotics in conjunction with the use of biofilm-active drugs.<\/jats:p>","DOI":"10.3390\/vetsci10050314","type":"journal-article","created":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T01:44:42Z","timestamp":1682473482000},"page":"314","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Limiting Factors in Treatment Success of Biofilm-Forming Streptococci in the Case of Canine Infective Endocarditis Caused by Streptococcus canis"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6950-5769","authenticated-orcid":false,"given":"Miriam","family":"Katsburg","sequence":"first","affiliation":[{"name":"Institute for Microbiology and Epizootics, Freie Universit\u00e4t Berlin, Robert-von-Ostertagstr. 7, 14163 Berlin, Germany"},{"name":"Veterinary Centre for Resistance Research (TZR), Freie Universit\u00e4t Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane","family":"Weingart","sequence":"additional","affiliation":[{"name":"Small Animal Clinic, Freie Universit\u00e4t Berlin, Oertzenweg 19b, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Aubry","sequence":"additional","affiliation":[{"name":"Institute for Microbiology and Epizootics, Freie Universit\u00e4t Berlin, Robert-von-Ostertagstr. 7, 14163 Berlin, Germany"},{"name":"Veterinary Centre for Resistance Research (TZR), Freie Universit\u00e4t Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3686-9993","authenticated-orcid":false,"given":"Olivia","family":"Kershaw","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Pathology, Freie Universit\u00e4t Berlin, Robert-von-Ostertagstr. 15, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Kikhney","sequence":"additional","affiliation":[{"name":"Biofilmcenter, Department for Microbiology, Infectious Diseases and Immunology, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Hindenburgdamm 30, 12203 Berlin, Germany"},{"name":"MoKi Analytics GmbH, Marienplatz 9, 12207 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1532-3334","authenticated-orcid":false,"given":"Laura","family":"Kursawe","sequence":"additional","affiliation":[{"name":"Biofilmcenter, Department for Microbiology, Infectious Diseases and Immunology, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Hindenburgdamm 30, 12203 Berlin, Germany"},{"name":"MoKi Analytics GmbH, Marienplatz 9, 12207 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antina","family":"L\u00fcbke-Becker","sequence":"additional","affiliation":[{"name":"Institute for Microbiology and Epizootics, Freie Universit\u00e4t Berlin, Robert-von-Ostertagstr. 7, 14163 Berlin, Germany"},{"name":"Veterinary Centre for Resistance Research (TZR), Freie Universit\u00e4t Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1318-5780","authenticated-orcid":false,"given":"Annette","family":"Moter","sequence":"additional","affiliation":[{"name":"Biofilmcenter, Department for Microbiology, Infectious Diseases and Immunology, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Hindenburgdamm 30, 12203 Berlin, Germany"},{"name":"MoKi Analytics GmbH, Marienplatz 9, 12207 Berlin, Germany"},{"name":"Moter Diagnostics, Marienplatz 9, 12207 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Skrodzki","sequence":"additional","affiliation":[{"name":"Small Animal Clinic, Freie Universit\u00e4t Berlin, Oertzenweg 19b, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Kohn","sequence":"additional","affiliation":[{"name":"Small Animal Clinic, Freie Universit\u00e4t Berlin, Oertzenweg 19b, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0469-3951","authenticated-orcid":false,"given":"Marcus","family":"Fulde","sequence":"additional","affiliation":[{"name":"Institute for Microbiology and Epizootics, Freie Universit\u00e4t Berlin, Robert-von-Ostertagstr. 7, 14163 Berlin, Germany"},{"name":"Veterinary Centre for Resistance Research (TZR), Freie Universit\u00e4t Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1038\/s41587-020-0439-x","article-title":"The nf-core framework for community-curated bioinformatics 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