{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T15:52:32Z","timestamp":1792684352727,"version":"4.5.0"},"reference-count":71,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T00:00:00Z","timestamp":1683763200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT (Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia)","award":["DFA\/BD\/5332\/2020"],"award-info":[{"award-number":["DFA\/BD\/5332\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Pseudomonas aeruginosa is a pathogenic bacterium that can cause serious infections in both humans and animals, including dogs. Treatment of this bacterium is challenging because some strains have developed multi-drug resistance. This study aimed to evaluate the antimicrobial resistance patterns and biofilm production of clinical isolates of P. aeruginosa obtained from dogs. The study found that resistance to various \u03b2-lactam antimicrobials was widespread, with cefovecin and ceftiofur showing resistance in 74% and 59% of the isolates tested, respectively. Among the aminoglycosides, all strains showed susceptibility to amikacin and tobramycin, while gentamicin resistance was observed in 7% of the tested isolates. Furthermore, all isolates carried the oprD gene, which is essential in governing the entry of antibiotics into bacterial cells. The study also investigated the presence of virulence genes and found that all isolates carried exoS, exoA, exoT, exoY, aprA, algD, and plcH genes. This study compared P. aeruginosa resistance patterns worldwide, emphasizing regional understanding and responsible antibiotic use to prevent multi-drug resistance from emerging. In general, the results of this study emphasize the importance of the continued monitoring of antimicrobial resistance in veterinary medicine.<\/jats:p>","DOI":"10.3390\/vetsci10050343","type":"journal-article","created":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T01:05:10Z","timestamp":1683853510000},"page":"343","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["The Impact of the Virulence of Pseudomonas aeruginosa Isolated from Dogs"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2326-6226","authenticated-orcid":false,"given":"Telma","family":"de Sousa","sequence":"first","affiliation":[{"name":"Department of Genetics and Biotechnology, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Functional Genomics and Proteomics Unit, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Associate Laboratory for Green Chemistry (LAQV), Chemistry Department, Faculty of Science and Technology, University Nova of Lisbon, 2829-516 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7940-3141","authenticated-orcid":false,"given":"Andreia","family":"Garc\u00eas","sequence":"additional","affiliation":[{"name":"CRL-CESPU, Cooperativa de Ensino Superior Polit\u00e9cnico e Universit\u00e1rio, R. Central Dada Gandra, 1317, 4585-116 Gandra, Portugal"},{"name":"CITAB, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3992-3771","authenticated-orcid":false,"given":"Augusto","family":"Silva","sequence":"additional","affiliation":[{"name":"INNO\u2014Veterinary Laboratory, R. C\u00e2ndido de Sousa 15, 4710-503 Braga, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9339-9932","authenticated-orcid":false,"given":"Ricardo","family":"Lopes","sequence":"additional","affiliation":[{"name":"INNO\u2014Veterinary Laboratory, R. 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Target. Ther."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"de Sousa, T., Hebraud, M., Dapkevicius, M., Maltez, L., Pereira, J.E., Capita, R., Alonso-Calleja, C., Igrejas, G., and Poeta, P. (2021). Genomic and Metabolic Characteristics of the Pathogenicity in Pseudomonas aeruginosa. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222312892"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6695","DOI":"10.1128\/JB.00023-07","article-title":"The autotransporter esterase EstA of Pseudomonas aeruginosa is required for rhamnolipid production, cell motility, and biofilm formation","volume":"189","author":"Wilhelm","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1002\/mbo3.301","article-title":"The LapG protein plays a role in Pseudomonas aeruginosa biofilm formation by controlling the presence of the CdrA adhesin on the cell surface","volume":"4","author":"Rybtke","year":"2015","journal-title":"Microbiologyopen"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1086\/427035","article-title":"Background for different use of antibiotics in different countries","volume":"40","author":"Llor","year":"2005","journal-title":"Clin. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.jbiotec.2014.09.003","article-title":"Antibiotic resistance in Pseudomonas aeruginosa biofilms: Towards the development of novel anti-biofilm therapies","volume":"191","author":"Taylor","year":"2014","journal-title":"J. Biotechnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"55","DOI":"10.4248\/IJOS11026","article-title":"The clinical impact of bacterial biofilms","volume":"3","author":"Hoiby","year":"2011","journal-title":"Int. J. Oral Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0005-2736(03)00059-2","article-title":"Protein secretion systems of Pseudomonas aeruginosa and P fluorescens","volume":"1611","author":"Ma","year":"2003","journal-title":"Biochim. Biophys. Acta (BBA)-Biomembr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1089\/ars.2014.5979","article-title":"Pseudomonas aeruginosa pyocyanin induces neutrophil death via mitochondrial reactive oxygen species and mitochondrial acid sphingomyelinase","volume":"22","author":"Manago","year":"2015","journal-title":"Antioxid. Redox Signal."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1128\/iai.60.2.328-336.1992","article-title":"Response of Pseudomonas aeruginosa to pyocyanin: Mechanisms of resistance, antioxidant defenses, and demonstration of a manganese-cofactored superoxide dismutase","volume":"60","author":"Hassett","year":"1992","journal-title":"Infect. Immun."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1016\/j.ijmm.2010.08.005","article-title":"Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons","volume":"300","author":"Bleves","year":"2010","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1038\/s41598-020-58335-6","article-title":"Isolation and characterization of nutrient dependent pyocyanin from Pseudomonas aeruginosa and its dye and agrochemical properties","volume":"10","author":"DeBritto","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.cvsm.2019.10.007","article-title":"Bacterial Pneumonia in Dogs and Cats: An Update","volume":"50","author":"Dear","year":"2020","journal-title":"Vet. Clin. Small Anim. Pract."},{"key":"ref_14","unstructured":"van Knapen, F., and Overgaauw, P. (2015). Zoonoses-Infections Affecting Humans and Animals: Focus on Public Health Aspects, Springer."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Haenni, M., Hocquet, D., Ponsin, C., Cholley, P., Guyeux, C., Madec, J.Y., and Bertrand, X. (2015). Population structure and antimicrobial susceptibility of Pseudomonas aeruginosa from animal infections in France. BMC Vet. Res., 11.","DOI":"10.1186\/s12917-015-0324-x"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1042\/bj0960739","article-title":"Cephalosporinase and penicillinase activities of a beta-lactamase from Pseudomonas pyocyanea","volume":"96","author":"Sabath","year":"1965","journal-title":"Biochem. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"27","DOI":"10.2323\/jgam.47.27","article-title":"Roles of MexXY- and MexAB-multidrug efflux pumps in intrinsic multidrug resistance of Pseudomonas aeruginosa PAO1","volume":"47","author":"Morita","year":"2001","journal-title":"J. Gen. Appl. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e00784-19","DOI":"10.1128\/AAC.00784-19","article-title":"Defects in Efflux (oprM), beta-Lactamase (ampC), and Lipopolysaccharide Transport (lptE) Genes Mediate Antibiotic Hypersusceptibility of Pseudomonas aeruginosa Strain Z61","volume":"63","author":"Shen","year":"2019","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"100056","DOI":"10.1016\/j.bioflm.2021.100056","article-title":"The many antibiotic resistance and tolerance strategies of Pseudomonas aeruginosa","volume":"3","author":"Sindeldecker","year":"2021","journal-title":"Biofilm"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1128\/AAC.49.2.479-487.2005","article-title":"Aminoglycoside resistance in Pseudomonas aeruginosa","volume":"49","author":"Poole","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_21","first-page":"303","article-title":"Mechanisms responsible for the emergence of carbapenem resistance in Pseudomonas aeruginosa","volume":"16","author":"Meletis","year":"2012","journal-title":"Hippokratia"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"725383","DOI":"10.3389\/fmicb.2021.725383","article-title":"Detection of Colistin Resistance in Pseudomonas aeruginosa Using the MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer","volume":"12","author":"Jeannot","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.3389\/fphar.2019.01146","article-title":"Synergistic Combination of Polymyxin B and Enrofloxacin Induced Metabolic Perturbations in Extensive Drug-Resistant Pseudomonas aeruginosa","volume":"10","author":"Lin","year":"2019","journal-title":"Front. Pharmacol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Karruli, A., Catalini, C., D\u2019Amore, C., Foglia, F., Mari, F., Harxhi, A., Galdiero, M., and Durante-Mangoni, E. (2023). Evidence-Based Treatment of Pseudomonas aeruginosa Infections: A Critical Reappraisal. Antibiotics, 12.","DOI":"10.3390\/antibiotics12020399"},{"key":"#cr-split#-ref_25.1","unstructured":"EFSA Panel on Animal Health and Welfare (AHAW), Nielsen, S.S., Bicout, D.J., Calistri, P., Canali, E., Drewe, J.A., Garin-Bastuji, B., Gonzales Rojas, J.L., Gortazar, C., and Herskin, M. (2022). Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation"},{"key":"#cr-split#-ref_25.2","unstructured":"(EU) No 2016\/429): Antimicrobial-resistant Pseudomonas aeruginosa in dogs and cats. EFSA J., 20."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1093\/jac\/dkh332","article-title":"Pet animals as reservoirs of antimicrobial-resistant bacteria","volume":"54","author":"Guardabassi","year":"2004","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.biotechadv.2018.11.013","article-title":"Antibiotic resistance in Pseudomonas aeruginosa: Mechanisms and alternative therapeutic strategies","volume":"37","author":"Pang","year":"2019","journal-title":"Biotechnol. Adv."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"O\u2019Toole, G.A. (2011). Microtiter dish biofilm formation assay. J. Vis. Exp., e2437.","DOI":"10.3791\/2437-v"},{"key":"ref_29","first-page":"117","article-title":"Heat treatment of bacteria: A simple method of DNA extraction for molecular techniques","volume":"41","author":"Dashti","year":"2009","journal-title":"Kuwait Med. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.12688\/f1000research.15316.1","article-title":"Molecular characterization of Pseudomonas aeruginosa isolates from Sudanese patients: A cross-sectional study","volume":"7","author":"Amoon","year":"2018","journal-title":"F1000Research"},{"key":"ref_31","first-page":"75","article-title":"Nucleotide sequences of the genes coding for the TEM-like \u03b2-lactamases IRT-1 and IRT-2 (formerly called TRI-1 and TRI-2)","volume":"120","author":"Belaaouaj","year":"1994","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2864","DOI":"10.1128\/JCM.39.8.2864-2872.2001","article-title":"Characterization of clinical isolates of Klebsiella pneumoniae from 19 laboratories using the National Committee for Clinical Laboratory Standards extended-spectrum \u03b2-lactamase detection methods","volume":"39","author":"Steward","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4264","DOI":"10.1128\/JCM.41.9.4264-4269.2003","article-title":"Multiple CTX-M-type extended-spectrum \u03b2-lactamases in nosocomial isolates of Enterobacteriaceae from a hospital in northern Italy","volume":"41","author":"Pagani","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2385","DOI":"10.1128\/AAC.47.8.2385-2392.2003","article-title":"Ambler class A extended-spectrum \u03b2-lactamases in Pseudomonas aeruginosa: Novel developments and clinical impact","volume":"47","author":"Weldhagen","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"359","DOI":"10.3343\/alm.2012.32.5.359","article-title":"Multiplex PCR for rapid detection of genes encoding class A carbapenemases","volume":"32","author":"Hong","year":"2012","journal-title":"Ann. Lab. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1016\/j.burns.2014.02.010","article-title":"Determination of extended spectrum beta-lactamases, metallo-beta-lactamases and AmpC-beta-lactamases among carbapenem resistant Pseudomonas aeruginosa isolated from burn patients","volume":"40","author":"Neyestanaki","year":"2014","journal-title":"Burns"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1093\/jac\/dkl481","article-title":"Multiplex PCR for rapid detection of genes encoding acquired metallo-\u03b2-lactamases","volume":"59","author":"Ellington","year":"2007","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"757","DOI":"10.3855\/jidc.2268","article-title":"blaIMP and blaVIM mediated carbapenem resistance in Pseudomonas and Acinetobacter species in India","volume":"6","author":"Amudhan","year":"2012","journal-title":"J. Infect. Dev. Ctries."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1590\/S1807-59322010000900002","article-title":"Metallo-beta-lactamases among imipenem-resistant Pseudomonas aeruginosa in a Brazilian university hospital","volume":"65","author":"Franco","year":"2010","journal-title":"Clinics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1128\/AAC.37.4.927","article-title":"Nomenclature of aminoglycoside resistance genes: A comment","volume":"37","author":"Vliegenthart","year":"1993","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_41","first-page":"8607647","article-title":"Molecular characterization of antimicrobial resistance in Escherichia coli from rabbit farms in Tai\u2019an, China","volume":"2018","author":"Zhao","year":"2018","journal-title":"BioMed Res. Int."},{"key":"ref_42","first-page":"e25952","article-title":"Investigation of OprD porin protein levels in carbapenem-resistant Pseudomonas aeruginosa isolates","volume":"8","author":"Terzi","year":"2015","journal-title":"Jundishapur J. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e15722","DOI":"10.5812\/ircmj.15722","article-title":"Virulence gene profiles of multidrug-resistant Pseudomonas aeruginosa isolated from Iranian hospital infections","volume":"16","author":"Fazeli","year":"2014","journal-title":"Iran. Red Crescent Med. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/j.resmic.2013.06.007","article-title":"Lagooning of wastewaters favors dissemination of clinically relevant Pseudomonas aeruginosa","volume":"164","author":"Petit","year":"2013","journal-title":"Res. Microbiol."},{"key":"ref_45","first-page":"274","article-title":"Genetic identification of Pseudomonas aeruginosa virulence genes among different isolates","volume":"7","author":"Khattab","year":"2015","journal-title":"J. Microb. Biochem. Technol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"5510","DOI":"10.1128\/JB.00268-11","article-title":"Pseudomonas aeruginosa tssC1 links type VI secretion and biofilm-specific antibiotic resistance","volume":"193","author":"Zhang","year":"2011","journal-title":"J. Bacteriol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1099\/jmm.0.45617-0","article-title":"Analysis of quorum sensing-deficient clinical isolates of Pseudomonas aeruginosa","volume":"53","author":"Schaber","year":"2004","journal-title":"J. Med. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.micpath.2016.07.013","article-title":"Molecular detection of virulence genes in Pseudomonas aeruginosa isolated from children with Cystic Fibrosis and burn wounds in Iran","volume":"99","author":"Faraji","year":"2016","journal-title":"Microb. Pathog."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3526","DOI":"10.1128\/JCM.41.8.3526-3531.2003","article-title":"Single-nucleotide-polymorphism mapping of the Pseudomonas aeruginosa type III secretion toxins for development of a diagnostic multiplex PCR system","volume":"41","author":"Ajayi","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1167\/iovs.03-0980","article-title":"Pseudomonas aeruginosa with lasI quorum-sensing deficiency during corneal infection","volume":"45","author":"Zhu","year":"2004","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_51","unstructured":"(2023, March 20). MSD Animal Health Portugal. Available online: https:\/\/www.msd-animal-health.pt\/."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1111\/j.1348-0421.2011.00416.x","article-title":"Characterization of Pseudomonas aeruginosa isolates from dogs and cats in Japan: Current status of antimicrobial resistance and prevailing resistance mechanisms","volume":"56","author":"Harada","year":"2012","journal-title":"Microbiol. Immunol."},{"key":"ref_53","first-page":"884","article-title":"Determining of resistance and sensitivity of Pseudomonas aeruginosa in Iran in 2010\u20132011","volume":"7","author":"Shahini","year":"2012","journal-title":"Res. Pharm. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Kocsis, B., Gulyas, D., and Szabo, D. (2021). Diversity and Distribution of Resistance Markers in Pseudomonas aeruginosa International High-Risk Clones. Microorganisms, 9.","DOI":"10.3390\/microorganisms9020359"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"978","DOI":"10.14202\/vetworld.2021.978-985","article-title":"Occurrence, antimicrobial susceptibility, and pathogenic factors of Pseudomonas aeruginosa in canine clinical samples","volume":"14","author":"Hattab","year":"2021","journal-title":"Vet. World"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2001359","DOI":"10.2807\/1560-7917.ES.2021.26.4.2001359","article-title":"Building the European antimicrobial resistance surveillance network in veterinary medicine (EARS-Vet)","volume":"26","author":"Mader","year":"2021","journal-title":"Eurosurveillance"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1292\/jvms.12-0239","article-title":"Contribution of the MexXY multidrug efflux pump and other chromosomal mechanisms on aminoglycoside resistance in Pseudomonas aeruginosa isolates from canine and feline infections","volume":"74","author":"Poonsuk","year":"2012","journal-title":"J. Vet. Med. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.3389\/fmicb.2017.01847","article-title":"Resistance of animal strains of Pseudomonas aeruginosa to carbapenems","volume":"8","author":"Haenni","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.ijmm.2011.10.001","article-title":"Structure and function of OprD protein in Pseudomonas aeruginosa: From antibiotic resistance to novel therapies","volume":"302","author":"Li","year":"2012","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1128\/JB.188.1.45-54.2006","article-title":"Role of the novel OprD family of porins in nutrient uptake in Pseudomonas aeruginosa","volume":"188","author":"Tamber","year":"2006","journal-title":"J. Bacteriol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1900970","DOI":"10.1183\/13993003.00970-2019","article-title":"Training dogs to differentiate Pseudomonas aeruginosa from other cystic fibrosis bacterial pathogens: Not to be sniffed at?","volume":"54","author":"Davies","year":"2019","journal-title":"Eur. Respir. J."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1167\/iovs.08-2358","article-title":"Pathogenic phenotype and genotype of Pseudomonas aeruginosa isolates from spontaneous canine ocular infections","volume":"50","author":"Ledbetter","year":"2009","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Pottier, M., Castagnet, S., Gravey, F., Leduc, G., S\u00e9vin, C., Petry, S., Giard, J.-C., Le Hello, S., and L\u00e9on, A. (2022). Antimicrobial Resistance and Genetic Diversity of Pseudomonas aeruginosa Strains Isolated from Equine and Other Veterinary Samples. Pathogens, 12.","DOI":"10.3390\/pathogens12010064"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"141","DOI":"10.2147\/IDR.S346313","article-title":"Epidemiology, molecular characteristics, and virulence factors of carbapenem-resistant Pseudomonas aeruginosa isolated from patients with urinary tract infections","volume":"15","author":"Park","year":"2022","journal-title":"Infect. Drug Resist."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"O\u2019Connor, K., Zhao, C.Y., and Diggle, S.P. (2021). Frequency of quorum sensing mutations in Pseudomonas aeruginosa strains isolated from different environments. bioRxiv.","DOI":"10.1101\/2021.02.22.432365"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Abidi, S.H., Sherwani, S.K., Siddiqui, T.R., Bashir, A., and Kazmi, S.U. (2013). Drug resistance profile and biofilm forming potential of Pseudomonas aeruginosa isolated from contact lenses in Karachi-Pakistan. BMC Ophthalmol., 13.","DOI":"10.1186\/1471-2415-13-57"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1038\/nprot.2010.110","article-title":"A 96-well-plate-based optical method for the quantitative and qualitative evaluation of Pseudomonas aeruginosa biofilm formation and its application to susceptibility testing","volume":"5","author":"Musken","year":"2010","journal-title":"Nat. Protoc."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Plokarz, D., Czopowicz, M., Bierowiec, K., and Rypula, K. (2022). Virulence Genes as Markers for Pseudomonas aeruginosa Biofilm Formation in Dogs and Cats. Animals, 12.","DOI":"10.3390\/ani12040422"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"446-e99","DOI":"10.1111\/vde.12040","article-title":"Evaluation of biofilm production by Pseudomonas aeruginosa from canine ears and the impact of biofilm on antimicrobial susceptibility in vitro","volume":"24","author":"Pye","year":"2013","journal-title":"Vet. Dermatol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1186\/s13567-022-01097-y","article-title":"Pseudomonas aeruginosa biofilm dispersion by the mouse antimicrobial peptide CRAMP","volume":"53","author":"Zhang","year":"2022","journal-title":"Vet. 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