{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T21:49:41Z","timestamp":1767649781985,"version":"build-2065373602"},"reference-count":68,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T00:00:00Z","timestamp":1685404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003624","name":"Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, and Forestry (IPET)","doi-asserted-by":"publisher","award":["121004-1","320005-04-4-SB080"],"award-info":[{"award-number":["121004-1","320005-04-4-SB080"]}],"id":[{"id":"10.13039\/501100003624","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003624","name":"Ministry of Agriculture, Food, and Rural Affairs (MAFRA)","doi-asserted-by":"publisher","award":["121004-1","320005-04-4-SB080"],"award-info":[{"award-number":["121004-1","320005-04-4-SB080"]}],"id":[{"id":"10.13039\/501100003624","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Rottlerin (R) is a natural extract from Mallotus philippensis with antiviral properties. Feline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus (FCoV) that is characterized by systemic granulomatous inflammation and high mortality. We investigated the antiviral effect of liposome-loaded R, i.e., rottlerin-liposome (RL), against FCoV. We demonstrated that RL inhibited FCoV replication in a dose-dependent manner, not only in the early endocytosis stage but also in the late stage of replication. RL resolved the low solubility issue of rottlerin and improved its inhibition efficacy at the cellular level. Based on these findings, we suggest that RL is worth further investigation as a potential treatment for FCoV.<\/jats:p>","DOI":"10.3390\/vetsci10060380","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T02:57:10Z","timestamp":1685501830000},"page":"380","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Rottlerin-Liposome Inhibits the Endocytosis of Feline Coronavirus Infection"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3099-6040","authenticated-orcid":false,"given":"Jong-Chul","family":"Choi","sequence":"first","affiliation":[{"name":"Qvet Co., Ltd., 606, Alumini Association Building of Konkuk University, 5 Achasan-ro 36-gil, Gwangjin-gu, Seoul 05066, Republic of Korea"},{"name":"Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung-Won","family":"Jung","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In-Yeong","family":"Choi","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeong-Lim","family":"Kang","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-Hun","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"},{"name":"KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang-Won","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"},{"name":"KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung-Yong","family":"Park","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"},{"name":"KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4158-6402","authenticated-orcid":false,"given":"Chang-Seon","family":"Song","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"},{"name":"KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In-Soo","family":"Choi","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"},{"name":"KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1074-8162","authenticated-orcid":false,"given":"Joong-Bok","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"},{"name":"KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2300-6360","authenticated-orcid":false,"given":"Changin","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Genetics, Yale School of Medicine, P.O. Box 208005, New Haven, CT 06520-8005, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.34172\/jhp.2021.03","article-title":"Mallotus philippensis (Lam.) M\u00fcll. Arg.: A Review on Its Pharmacology and Phytochemistry","volume":"10","author":"Kumar","year":"2020","journal-title":"J. Herbmed. Pharmacol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1007\/s11101-009-9152-6","article-title":"Mallotus Species from Vietnamese Mountainous Areas: Phytochemistry and Pharmacological Activities","volume":"9","author":"Dejaegher","year":"2010","journal-title":"Phytochem. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1006\/bbrc.1994.1199","article-title":"Rottlerin, a Novel Protein Kinase Inhibitor","volume":"199","author":"Gschwendt","year":"1994","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_4","first-page":"89","article-title":"A Review of Kampillaka (Mallotus Philippinensis Muell) from Ayurvedic Perspective","volume":"2","author":"Kumar","year":"2013","journal-title":"Ann. Ayurvedic Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"32637","DOI":"10.1021\/acsomega.1c04266","article-title":"Pharmacokinetic Assessment of Rottlerin from Mallotus Philippensis Using a Highly Sensitive Liquid Chromatography\u2013Tandem Mass Spectrometry-Based Bioanalytical Method","volume":"6","author":"Manhas","year":"2021","journal-title":"ACS Omega"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.virs.2022.07.012","article-title":"Rottlerin Plays an Antiviral Role at Early and Late Steps of Zika Virus Infection","volume":"37","author":"Zhou","year":"2022","journal-title":"Virol. Sin."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1038\/s41598-017-01525-6","article-title":"Rottlerin-Mediated Inhibition of Toxoplasma gondii Growth in BeWo Trophoblast-like Cells","volume":"7","author":"Ietta","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/j.bbrc.2016.08.014","article-title":"Protein Kinase C-\u03b4 Inhibitor, Rottlerin Inhibits Growth and Survival of Mycobacteria Exclusively through Shikimate Kinase","volume":"478","author":"Pandey","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1128\/AEM.02151-07","article-title":"Rottlerin Inhibits Chlamydial Intracellular Growth and Blocks Chlamydial Acquisition of Sphingolipids from Host Cells","volume":"74","author":"Shivshankar","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/1742-4690-9-37","article-title":"Protein Kinase C-Delta Regulates HIV-1 Replication at an Early Post-Entry Step in Macrophages","volume":"9","author":"Contreras","year":"2012","journal-title":"Retrovirology"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Filone, C.M., Hanna, S.L., Caino, M.C., Bambina, S., Doms, R.W., and Cherry, S. (2010). Rift Valley Fever Virus Infection of Human Cells and Insect Hosts Is Promoted by Protein Kinase C Epsilon. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0015483"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"105191","DOI":"10.1016\/j.antiviral.2021.105191","article-title":"Inhibition of Endocytosis of Porcine Reproductive and Respiratory Syndrome Virus by Rottlerin and Its Potential Prophylactic Administration in Piglets","volume":"195","author":"Kang","year":"2021","journal-title":"Antiviral. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1609","DOI":"10.3892\/ijo.2015.2848","article-title":"Activation of PKC-\u03b4 in HTLV-1-Infected T Cells","volume":"46","author":"Mori","year":"2015","journal-title":"Int. J. Oncol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.virol.2014.07.040","article-title":"PKC\u03b4 Is Required for Porcine Reproductive and Respiratory Syndrome Virus Replication","volume":"468\u2013470","author":"Zhao","year":"2014","journal-title":"Virology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.tips.2007.07.003","article-title":"Rottlerin: An Inappropriate and Ineffective Inhibitor of PKC\u03b4","volume":"28","author":"Soltoff","year":"2007","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.antiviral.2019.04.014","article-title":"Kinase Inhibitors Tyrphostin 9 and Rottlerin Block Early Steps of Rabies Virus Cycle","volume":"168","author":"Lama","year":"2019","journal-title":"Antivir. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1007\/s00125-010-1668-1","article-title":"Protein Kinase C-\u03b4 Is Involved in the Inflammatory Effect of IL-6 in Mouse Adipose Cells","volume":"53","author":"Wallerstedt","year":"2010","journal-title":"Diabetologia"},{"key":"ref_18","unstructured":"(2019). Lee; Kim; Lee; Kwon PKC\u03b4 Mediates NF-\u039aB Inflammatory Response and Downregulates SIRT1 Expression in Liver Fibrosis. Int. J. Mol. Sci., 20."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1038\/mi.2017.68","article-title":"Protein Kinase C-Delta (PKC\u03b4), a Marker of Inflammation and Tuberculosis Disease Progression in Humans, Is Important for Optimal Macrophage Killing Effector Functions and Survival in Mice","volume":"11","author":"Parihar","year":"2018","journal-title":"Mucosal Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1038\/s41401-019-0281-1","article-title":"Overview of Recent Advances in Liposomal Nanoparticle-Based Cancer Immunotherapy","volume":"40","author":"Gao","year":"2019","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"12530","DOI":"10.1002\/jcp.28120","article-title":"Nanovaccine: A Novel Approach in Immunization","volume":"234","author":"Darroudi","year":"2019","journal-title":"J. Cell Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"13043","DOI":"10.1038\/s41598-017-13316-0","article-title":"Nanoparticulate Vacuolar ATPase Blocker Exhibits Potent Host-Targeted Antiviral Activity against Feline Coronavirus","volume":"7","author":"Hu","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1016\/j.tips.2009.08.004","article-title":"Liposomes and Nanoparticles: Nanosized Vehicles for Drug Delivery in Cancer","volume":"30","author":"Malam","year":"2009","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_24","first-page":"3087","article-title":"Comparative Study of the in Vitro and in Vivo Characteristics of Cationic and Neutral Liposomes","volume":"6","author":"Zhao","year":"2011","journal-title":"Int. J. Nanomed."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"102147","DOI":"10.1016\/j.jddst.2020.102147","article-title":"Liposomal Delivery Systems for Herbal Extracts","volume":"61","author":"Sogut","year":"2021","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Olusanya, T., Haj Ahmad, R., Ibegbu, D., Smith, J., and Elkordy, A. (2018). Liposomal Drug Delivery Systems and Anticancer Drugs. Molecules, 23.","DOI":"10.3390\/molecules23040907"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/B978-0-444-63930-1.00010-7","article-title":"Chemistry, Bioactivity, and the Structure-Activity Relationship of Cephalotaxine-Type Alkaloids From Cephalotaxus sp","volume":"53","author":"Chang","year":"2017","journal-title":"Stud. Nat. Prod. Chem."},{"key":"ref_28","first-page":"1823","article-title":"Development of a Liposome-Based Formulation for Vitamin K1 Nebulization on the Skin","volume":"9","author":"Campani","year":"2014","journal-title":"Int. J. Nanomed."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1021\/jf402886s","article-title":"Characterization and Bioavailability of Tea Polyphenol Nanoliposome Prepared by Combining an Ethanol Injection Method with Dynamic High-Pressure Microfluidization","volume":"62","author":"Zou","year":"2014","journal-title":"J. Agric. Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4942","DOI":"10.1128\/JVI.03688-14","article-title":"Broad-Spectrum Inhibitors against 3C-Like Proteases of Feline Coronaviruses and Feline Caliciviruses","volume":"89","author":"Kim","year":"2015","journal-title":"J. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"14122","DOI":"10.1128\/JVI.79.22.14122-14130.2005","article-title":"Acquisition of Macrophage Tropism during the Pathogenesis of Feline Infectious Peritonitis Is Determined by Mutations in the Feline Coronavirus Spike Protein","volume":"79","author":"Rottier","year":"2005","journal-title":"J. Virol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.tvjl.2014.04.016","article-title":"An Update on Feline Infectious Peritonitis: Diagnostics and Therapeutics","volume":"201","author":"Pedersen","year":"2014","journal-title":"Vet. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.vetmic.2014.10.025","article-title":"Levels of Feline Infectious Peritonitis Virus in Blood, Effusions, and Various Tissues and the Role of Lymphopenia in Disease Outcome Following Experimental Infection","volume":"175","author":"Pedersen","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Kim, Y., Liu, H., Galasiti Kankanamalage, A.C., Weerasekara, S., Hua, D.H., Groutas, W.C., Chang, K.O., and Pedersen, N.C. (2016). Reversal of the Progression of Fatal Coronavirus Infection in Cats by a Broad-Spectrum Coronavirus Protease Inhibitor. PLoS Pathog., 12.","DOI":"10.1371\/journal.ppat.1005531"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.jfms.2010.09.014","article-title":"Clinicopathological Findings and Disease Staging of Feline Infectious Peritonitis: 51 Cases from 2003 to 2009 in Taiwan","volume":"13","author":"Tsai","year":"2011","journal-title":"J. Feline Med. Surg."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1016\/j.jfms.2008.12.002","article-title":"Effect of Polyprenyl Immunostimulant on the Survival Times of Three Cats with the Dry Form of Feline Infectious Peritonitis","volume":"11","author":"Legendre","year":"2009","journal-title":"J. Feline Med. Surg."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.jfms.2003.12.009","article-title":"Recommendations from Workshops of the Second International Feline Coronavirus\/Feline Infectious Peritonitis Symposium","volume":"6","author":"Addie","year":"2004","journal-title":"J. Feline Med. Surg."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1016\/j.jfms.2009.05.008","article-title":"Feline Infectious Peritonitis: ABCD Guidelines on Prevention and Management","volume":"11","author":"Addie","year":"2009","journal-title":"J. Feline Med. Surg."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.antiviral.2013.04.016","article-title":"Effect of Chloroquine on Feline Infectious Peritonitis Virus Infection in Vitro and in Vivo","volume":"99","author":"Takano","year":"2013","journal-title":"Antivir. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1177\/1098612X19825701","article-title":"Efficacy and Safety of the Nucleoside Analog GS-441524 for Treatment of Cats with Naturally Occurring Feline Infectious Peritonitis","volume":"21","author":"Pedersen","year":"2019","journal-title":"J. Feline Med. Surg."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/0378-1135(89)90049-7","article-title":"Inhibitory Effects of Ribavirin Alone or Combined with Human Alpha Interferon on Feline Infectious Peritonitis Virus Replication in Vitro","volume":"20","author":"Weiss","year":"1989","journal-title":"Vet. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Doki, T., Tarusawa, T., Hohdatsu, T., and Takano, T. (2020). In Vivo Antiviral Effects of U18666A Against Type I Feline Infectious Peritonitis Virus. Pathogens, 9.","DOI":"10.3390\/pathogens9010067"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/s13567-019-0625-3","article-title":"Antiviral Activity of Itraconazole against Type I Feline Coronavirus Infection","volume":"50","author":"Takano","year":"2019","journal-title":"Vet. Res."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Roy, M., Jacque, N., Novicoff, W., Li, E., Negash, R., and Evans, S.J.M. (2022). Unlicensed Molnupiravir Is an Effective Rescue Treatment Following Failure of Unlicensed GS-441524-like Therapy for Cats with Suspected Feline Infectious Peritonitis. Pathogens, 11.","DOI":"10.3390\/pathogens11101209"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Yu, J., Kimble, B., Norris, J.M., and Govendir, M. (2020). Pharmacokinetic Profile of Oral Administration of Mefloquine to Clinically Normal Cats: A Preliminary In-Vivo Study of a Potential Treatment for Feline Infectious Peritonitis (FIP). Animals, 10.","DOI":"10.3390\/ani10061000"},{"key":"ref_46","first-page":"2580","article-title":"Pathogenicity Studies of Feline Coronavirus Isolates 79-1146 and 79-1683","volume":"45","author":"Pedersen","year":"1984","journal-title":"Am. J. Vet. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1177\/1040638718756460","article-title":"Comparison of the Performance of Laboratory Tests in the Diagnosis of Feline Infectious Peritonitis","volume":"30","author":"Stranieri","year":"2018","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.virol.2018.02.021","article-title":"Porcine Reproductive and Respiratory Syndrome Virus Induces HMGB1 Secretion via Activating PKC-Delta to Trigger Inflammatory Response","volume":"518","author":"Wang","year":"2018","journal-title":"Virology"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"21509","DOI":"10.1002\/er.7153","article-title":"Development of Dye-Sensitized Solar Cell Using M. Philippensis (Kamala Tree) Fruit Extract: A Combined Experimental and Theoretical Study","volume":"45","author":"Mahapatra","year":"2021","journal-title":"Int. J. Energy Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 Years of Image Analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1099\/jgv.0.001047","article-title":"Coronavirus S Protein-Induced Fusion Is Blocked Prior to Hemifusion by Abl Kinase Inhibitors","volume":"99","author":"Sisk","year":"2018","journal-title":"J. Gen. Virol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.antiviral.2016.12.020","article-title":"Protein Kinases C as Potential Host Targets for the Inhibition of Chikungunya Virus Replication","volume":"139","author":"Abdelnabi","year":"2017","journal-title":"Antivir. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1398","DOI":"10.1038\/s41564-021-00968-y","article-title":"Rottlerin Inhibits La Crosse Virus-Induced Encephalitis in Mice and Blocks Release of Replicating Virus from the Golgi Body in Neurons","volume":"6","author":"Ojha","year":"2021","journal-title":"Nat. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e10239","DOI":"10.15252\/msb.202110239","article-title":"A Systems-level Study Reveals Host-targeted Repurposable Drugs against SARS-CoV-2 Infection","volume":"17","author":"Chen","year":"2021","journal-title":"Mol. Syst. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e01056-22","DOI":"10.1128\/spectrum.01056-22","article-title":"Protein Kinase C Inhibitors Reduce SARS-CoV-2 Replication in Cultured Cells","volume":"10","author":"Huang","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e00184-20","DOI":"10.1128\/JVI.00184-20","article-title":"Macropinocytosis and Clathrin-Dependent Endocytosis Play Pivotal Roles for the Infectious Entry of Puumala Virus","volume":"94","author":"Bauherr","year":"2020","journal-title":"J. Virol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"4434","DOI":"10.1128\/JVI.03274-14","article-title":"ATP1A1-Mediated Src Signaling Inhibits Coronavirus Entry into Host Cells","volume":"89","author":"Burkard","year":"2015","journal-title":"J. Virol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"350826","DOI":"10.1100\/2012\/350826","article-title":"Rottlerin and Cancer: Novel Evidence and Mechanisms","volume":"2012","author":"Maioli","year":"2012","journal-title":"Sci. World J."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"eaav4580","DOI":"10.1126\/sciadv.aav4580","article-title":"A Pan-Coronavirus Fusion Inhibitor Targeting the HR1 Domain of Human Coronavirus Spike","volume":"5","author":"Xia","year":"2019","journal-title":"Sci. Adv."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/0042-6822(91)90045-D","article-title":"The S2 Subunit of the Spike Glycoprotein of Bovine Coronavirus Mediates Membrane Fusion in Insect Cells","volume":"180","author":"Yoo","year":"1991","journal-title":"Virology"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2439","DOI":"10.2147\/IJN.S227805","article-title":"Novel Drug Delivery Systems for Loading of Natural Plant Extracts and Their Biomedical Applications","volume":"15","author":"Rahman","year":"2020","journal-title":"Int. J. Nanomed."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"727241","DOI":"10.1155\/2011\/727241","article-title":"Targeted Liposomal Drug Delivery to Monocytes and Macrophages","volume":"2011","author":"Kelly","year":"2011","journal-title":"J. Drug Deliv."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jctube.2017.02.001","article-title":"Histopathologic Review of Granulomatous Inflammation","volume":"7","author":"Shah","year":"2017","journal-title":"J. Clin. Tuberc. Other Mycobact. Dis."},{"key":"ref_64","first-page":"687","article-title":"Mechanisms of Granulomatous Inflammation","volume":"46","author":"Epstein","year":"1989","journal-title":"Immunol. Ser."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Delaplace, M., Huet, H., Gambino, A., and Le Poder, S. (2021). Feline Coronavirus Antivirals: A Review. Pathogens, 10.","DOI":"10.3390\/pathogens10091150"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1136\/vr.135.8.177","article-title":"Antiviral Studies of Feline Infectious Peritonitis Virus in Vitro","volume":"135","author":"Barlough","year":"1994","journal-title":"Vet. Rec."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.antiviral.2010.06.010","article-title":"Synergistic Antiviral Effect of Galanthus Nivalis Agglutinin and Nelfinavir against Feline Coronavirus","volume":"88","author":"Hsieh","year":"2010","journal-title":"Antivir. Res."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Takano, T., Satoh, K., Doki, T., Tanabe, T., and Hohdatsu, T. (2020). Antiviral Effects of Hydroxychloroquine and Type I Interferon on In Vitro Fatal Feline Coronavirus Infection. Viruses, 12.","DOI":"10.3390\/v12050576"}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/6\/380\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:45:28Z","timestamp":1760125528000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/6\/380"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,30]]},"references-count":68,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["vetsci10060380"],"URL":"https:\/\/doi.org\/10.3390\/vetsci10060380","relation":{},"ISSN":["2306-7381"],"issn-type":[{"type":"electronic","value":"2306-7381"}],"subject":[],"published":{"date-parts":[[2023,5,30]]}}}