{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T19:24:05Z","timestamp":1785266645201,"version":"3.55.0"},"reference-count":35,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T00:00:00Z","timestamp":1694131200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>African swine fever virus is considered an emerging virus that causes African swine fever, a disease characterised by high mortality and elevated transmission rates and that, as it is for most other viral diseases, cannot be treated with specific drugs. Effective and reliable detection of the virus is relevant to prevent uncontrolled contagion among boar populations and to reduce economic losses. Moreover, animal health laboratories are demanding standardisation, optimisation and quality assurance of the available diagnostic assays. In the present study, the ASFV MONODOSE dtec-qPCR kit was validated following the UNE-EN ISO\/IEC 17025:2005 guidelines. Analytical validation terms include in silico and in vitro specificity, sensitivity, efficiency and reliability (repeatability\/reproducibility). Diagnostic validation of the method was assessed through the analysis of a total of 181 porcine samples originating from six different matrix types doped with African swine fever virus DNA received from the European reference laboratory for African Swine Fever (INIA-CISA, Madrid, Spain): whole blood, blood serum, kidney, heart, liver and tonsil. Results agreed with those obtained from a reference detection method also based on real-time PCR, endorsed by WOAH, but the ASFV MONODOSE dtec-qPCR kit incorporates some technical innovations and improvements which may benefit end-users. This kit, available worldwide with full analytical and diagnostic validation, can recognise all known ASFV genotypes and brings additional benefits to the current qPCR technology.<\/jats:p>","DOI":"10.3390\/vetsci10090564","type":"journal-article","created":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T09:11:18Z","timestamp":1694423478000},"page":"564","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Internal Validation of the ASFV MONODOSE dtec-qPCR Kit for African Swine Fever Virus Detection under the UNE-EN ISO\/IEC 17025:2005 Criteria"],"prefix":"10.3390","volume":"10","author":[{"given":"Gema","family":"Bru","sequence":"first","affiliation":[{"name":"Genetic PCR Solutions\u2122, 03300 Orihuela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Mart\u00ednez-Candela","sequence":"additional","affiliation":[{"name":"Genetic PCR Solutions\u2122, 03300 Orihuela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paloma","family":"Romero","sequence":"additional","affiliation":[{"name":"Genetic PCR Solutions\u2122, 03300 Orihuela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Navarro","sequence":"additional","affiliation":[{"name":"Genetic PCR Solutions\u2122, 03300 Orihuela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1550-9590","authenticated-orcid":false,"given":"Antonio","family":"Mart\u00ednez-Murcia","sequence":"additional","affiliation":[{"name":"Genetic PCR Solutions\u2122, 03300 Orihuela, Spain"},{"name":"Department of Microbiology, University Miguel Hern\u00e1ndez, 03312 Orihuela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1099\/jgv.0.001049","article-title":"ICTV Virus Taxonomy Profile: Asfarviridae","volume":"99","author":"Alonso","year":"2018","journal-title":"J. Gen. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Borca, M.V., Ramirez-Medina, E., Silva, E., Vuono, E., Rai, A., Pruitt, S., Espinoza, N., Velazquez-Salinas, L., Gay, C.G., and Gladue, D.P. (2021). ASFV-G-\u2206I177L as an Effective Oral Nasal Vaccine against the Eurasia Strain of Africa Swine Fever. Viruses, 13.","DOI":"10.3390\/v13050765"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.virusres.2019.03.022","article-title":"Development of vaccines against African swine fever virus","volume":"265","author":"Revilla","year":"2019","journal-title":"Virus Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1112850","DOI":"10.3389\/fvets.2023.1112850","article-title":"A multi gene-approach genotyping method identifies 24 genetic clusters within the genotype II-European African swine fever viruses circulating from 2007 to 2022","volume":"10","author":"Gallardo","year":"2023","journal-title":"Front. Vet. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1111\/tbed.12700","article-title":"Genetic characterization of African swine fever virus isolates from soft ticks at the wildlife\/domestic interface in Mozambique and identification of a novel genotype","volume":"65","author":"Quembo","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1111\/tbed.12511","article-title":"Identification of a New Genotype of African Swine Fever Virus in Domestic Pigs from Ethiopia","volume":"64","author":"Achenbach","year":"2017","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.jcpa.2014.09.003","article-title":"An Update on the Epidemiology and Pathology of African Swine Fever","volume":"152","author":"Mur","year":"2015","journal-title":"J. Comp. Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"215","DOI":"10.3389\/fvets.2020.00215","article-title":"African Swine Fever Virus: An Emerging DNA Arbovirus","volume":"7","author":"Gaudreault","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-Cord\u00f3n, P.J., Nunez, A., Neimanis, A., Wikstr\u00f6m-Lassa, E., Montoya, M., Crooke, H., and Gavier-Wid\u00e9n, D. (2019). African Swine Fever: Disease Dynamics in Wild Boar Experimentally Infected with ASFV Isolates Belonging to Genotype I and II. Viruses, 11.","DOI":"10.3390\/v11090852"},{"key":"ref_10","unstructured":"WOAH\u2014World Organisation for Animal Health (2023, January 31). African Swine Fever (ASF)\u2014Situation Report 26 n.d.. Available online: https:\/\/www.woah.org\/en\/document\/african-swine-fever-asf-situation-report-26\/."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"197676","DOI":"10.1016\/j.virusres.2019.197676","article-title":"African swine fever (ASF) diagnosis, an essential tool in the epidemiological investigation","volume":"271","author":"Gallardo","year":"2019","journal-title":"Virus Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1007\/s12275-019-8457-4","article-title":"Genetic characterization of African swine fever virus in Cameroon, 2010\u20132018","volume":"57","author":"Wade","year":"2019","journal-title":"J. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s13028-016-0264-x","article-title":"Preventive measures aimed at minimizing the risk of African swine fever virus spread in pig farming systems","volume":"58","author":"Bellini","year":"2016","journal-title":"Acta Vet. Scand."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1556\/avet.53.2005.1.11","article-title":"The molecular diagnosis of porcine viral diseases: A review","volume":"53","year":"2005","journal-title":"Acta Vet. Hung."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.clinmicnews.2007.05.002","article-title":"Real-time PCR in clinical microbiology: Verification, validation, and contamination control","volume":"29","author":"Sloan","year":"2007","journal-title":"Clin. Microbiol. Newsl."},{"key":"ref_16","unstructured":"WOAH\u2014World Organisation for Animal Health (2023, February 01). Terrestrial Manual Online Access n.d. Available online: https:\/\/www.woah.org\/en\/what-we-do\/standards\/codes-and-manuals\/terrestrial-manual-online-access\/."},{"key":"ref_17","unstructured":"WOAH\u2014World Organisation for Animal Health (2023, July 01). Available online: https:\/\/www.woah.org\/fileadmin\/Home\/fr\/Health_standards\/tahm\/3.08.01_ASF.pdf."},{"key":"ref_18","unstructured":"Beltr\u00e1n-Alcrudo, D., Arias, M., Gallardo, C., Kramer, S.A., and Penrith, M.-L. (2017). African Swine Fever: Detection and Diagnosis:  A Manual for Veterinarians, Food and Agriculture Organization of the United Nations."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.jviromet.2011.09.007","article-title":"Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus","volume":"178","author":"Tignon","year":"2011","journal-title":"J. Virol. Methods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4431","DOI":"10.1128\/JCM.41.9.4431-4434.2003","article-title":"Highly Sensitive PCR Assay for Routine Diagnosis of African Swine Fever Virus in Clinical Samples","volume":"41","author":"Romero","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"159","DOI":"10.4236\/ajmb.2014.43017","article-title":"Polymerase Chain Reaction Detection of ASFV Infection in Nigerian Indigenous Pig","volume":"4","author":"Oluwole","year":"2014","journal-title":"Am. J. Mol. Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0166-0934(02)00189-1","article-title":"Development of a TaqMan\u00ae PCR assay with internal amplification control for the detection of African swine fever virus","volume":"107","author":"King","year":"2003","journal-title":"J. Virol. Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1128\/JCM.43.1.112-119.2005","article-title":"Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay","volume":"43","author":"Zsak","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Haines, F.J., Hofmann, M.A., King, D.P., Drew, T.W., and Crooke, H.R. (2013). Development and validation of a multiplex, real-time RT PCR assay for the simultaneous detection of classical and African swine fever viruses. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0071019"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1177\/1040638715574768","article-title":"Detection of African swine fever, classical swine fever, and foot-and-mouth disease viruses in swine oral fluids by multiplex reverse transcription real-time polymerase chain reaction","volume":"27","author":"Grau","year":"2015","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1111\/j.1865-1682.2012.01317.x","article-title":"Molecular diagnosis of African Swine Fever by a new real-time PCR using universal probe library","volume":"60","author":"Gallardo","year":"2013","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_27","unstructured":"(2023, July 01). UNE-EN ISO\/IEC 17025. 2005. Conformity Assessment. General Requirements for the Competence of Testing and Calibration Laboratories. Available online: https:\/\/www.iso.org\/standard\/39883.html."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1111\/myc.12834","article-title":"Internal validation of GPSTM MONODOSE CanAur dtec-qPCR kit following the UNE\/EN ISO\/IEC 17025, 2005 for detection of the emerging yeast Candida auris","volume":"61","author":"Navarro","year":"2018","journal-title":"Mycoses"},{"key":"ref_29","unstructured":"(2022, November 30). National Center for Biotechnology Information n.d., Available online: https:\/\/www.ncbi.nlm.nih.gov\/."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11, Molecular Evolutionary Genetics Analysis Version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/jam.14781","article-title":"Comparative in silico design and validation of GPSTM COVID-19 dtec-RT-qPCR test","volume":"130","author":"Bru","year":"2021","journal-title":"J. Appl. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-1135(98)00187-4","article-title":"African swine fever virus infection of the bushpig (Potamochoerus porcus) and its significance in the epidemiology of the disease","volume":"62","author":"Anderson","year":"1998","journal-title":"Vet. Microbiol."},{"key":"ref_33","unstructured":"(2002). Council Directive 2002\/60\/EC of 27 June 2002 laying down specific provisions for the control of African swine fever and amending Directive 92\/119\/EEC as regards Teschen disease and African swine fever (Text with EEA relevance). Off. J. Eur. Community, 192, 27\u201346."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.rvsc.2020.06.009","article-title":"African swine fever: A review of cleaning and disinfection procedures in commercial pig holdings","volume":"132","author":"Borella","year":"2020","journal-title":"Res. Vet. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2555","DOI":"10.1128\/JCM.00857-15","article-title":"Assessment of African Swine Fever Diagnostic Techniques as a Response to the Epidemic Outbreaks in Eastern European Union Countries: How to Improve Surveillance and Control Programs","volume":"53","author":"Gallardo","year":"2015","journal-title":"J. Clin. Microbiol."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/9\/564\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:47:41Z","timestamp":1760129261000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/9\/564"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,8]]},"references-count":35,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["vetsci10090564"],"URL":"https:\/\/doi.org\/10.3390\/vetsci10090564","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,8]]}}}