{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T08:05:22Z","timestamp":1767773122888,"version":"build-2065373602"},"reference-count":81,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T00:00:00Z","timestamp":1694476800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ARIMNET2, FP7-ERANET-2013","award":["618127"],"award-info":[{"award-number":["618127"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The supplementation of cryopreservation media with antioxidants improves the post-thaw quality and fertilizing ability of spermatozoa. To maximize the fertility of frozen\u2013thawed buck spermatozoa, further research is required to overcome obstacles that have yielded controversial results and standardize protocols. In the present work, the effect of adding fumaric acid (a well-described antioxidant) to a soy lecithin semen extender on certain quality parameters of spermatozoa following freezing and thawing was examined for the first time. Five sexually mature Skopelos bucks were used, and ejaculates were collected with an artificial vagina. The semen samples (98 samples, five replicates) were diluted (400 \u00d7 106 spermatozoa\/mL) with OviXcell\u00ae, supplemented with fumaric acid (0 mM, 2.15 mM, 10 mM or 30 mM), equilibrated (5 \u00b0C; 3 h), packed (0.5 mL straws), frozen and stored (\u2212196 \u00b0C) until further processing. After thawing, the spermatozoa total and progressive motility (CASA), viability (eosin\u2013nigrosin), membrane functional integrity (HOST), acrosome integrity (SpermBlue\u00ae) and mitochondrial function (Rhodamine-123\/SYBR-14\/PI) were evaluated. Statistical analysis was performed with one-way ANOVA, followed by Duncan\u2019s test; significance was set at 0.05. The addition of 2.15 mM fumaric acid improved (p &lt; 0.05) spermatozoa viability, membrane functional integrity, acrosome integrity and mitochondrial function compared to all other concentrations. The addition of 30 mM fumaric acid decreased (p &lt; 0.05) spermatozoa viability and mitochondrial function compared to all other concentrations. These results indicate a beneficial effect of a 2.15 mM fumaric acid addition to a soy lecithin extender on post-thaw buck spermatozoa quality. Further research is required to evaluate the in vivo fertility of frozen\u2013thawed buck spermatozoa treated with fumaric acid, as well as to elucidate the mechanism of action of fumaric acid in spermatozoa.<\/jats:p>","DOI":"10.3390\/vetsci10090569","type":"journal-article","created":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T21:41:12Z","timestamp":1694554872000},"page":"569","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Post-Thaw Parameters of Buck Semen Quality after Soy Lecithin Extender Supplementation with Fumaric Acid"],"prefix":"10.3390","volume":"10","author":[{"given":"Aikaterini","family":"Saratsi","sequence":"first","affiliation":[{"name":"Veterinary Research Institute, Hellenic Agricultural Organization\u2014DIMITRA, ELGO Campus, 57001 Thermi-Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Foteini","family":"Samartzi","sequence":"additional","affiliation":[{"name":"Veterinary Research Institute, Hellenic Agricultural Organization\u2014DIMITRA, ELGO Campus, 57001 Thermi-Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ioannis","family":"Panagiotidis","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction & Artificial Insemination, Directorate of Veterinary Center of Thessaloniki, Ministry of Rural Development and Food, 9 Verias Str., 57008 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4752-6654","authenticated-orcid":false,"given":"Athina","family":"Basioura","sequence":"additional","affiliation":[{"name":"Department of Agriculture, University of Western Macedonia, Terma Kontopoulou, 53100 Florina, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2319-1738","authenticated-orcid":false,"given":"Dimitrios","family":"Tsiokos","sequence":"additional","affiliation":[{"name":"Research Institute of Animal Science, Hellenic Agricultural Organization\u2014DIMITRA, 58100 Paralimni Giannitsa, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Ligda","sequence":"additional","affiliation":[{"name":"Veterinary Research Institute, Hellenic Agricultural Organization\u2014DIMITRA, ELGO Campus, 57001 Thermi-Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constantinos A.","family":"Rekkas","sequence":"additional","affiliation":[{"name":"Veterinary Research Institute, Hellenic Agricultural Organization\u2014DIMITRA, ELGO Campus, 57001 Thermi-Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5958\/0973-9718.2016.00005.2","article-title":"Cryopreservation of Goat Semen: Status and Prospects","volume":"22","author":"Gangwar","year":"2016","journal-title":"Indian J. 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